ライフサイエンスコーパス: EF-2

1 rom ADP-ribosylation of elongation factor 2 (EF-2).

2 croM), and no Ca(2+) binding was detected at EF-2.

3 enzymes such as elongation factor 1alpha and EF-2.

4 ep, via phosphorylation/dephosphorylation of EF-2.

5 tase-2A, the principal phosphatase acting on EF-2.

6 ti-EF-3 interaction by EF-1 alpha but not by EF-2.

7 nition of the ADP-ribose acceptor substrate, EF-2.

8 h appears to be presented for recognition by EF-2.

9 The apparent competition between EF-2 and EF-3 may represent binding of these two protein

10 lcium ions to the second and third EF-hands (EF-2 and EF-3) of recoverin leads to the extrusion of th

11 the calcium ion binds to EF-4 in addition to EF-2 and EF-3.

12 dependent both on the ratio of ribosomes to EF-2 and on the nature of the ribosomes.

13 ell-shaped dose-response curve, whereas when EF-2 and ribosomes were in equimolar concentrations sord

14 to abolish high-affinity calcium binding to EF-2 and thereby trap the myristoylated protein with cal

15 otes phosphorylation of elongation factor-2 (EF-2) and prostacyclin production, but not phosphorylati

16 nd that DTM has no ability to ADP-ribosylate EF-2 at 18 or 30 degrees C.

17 ed to be a regulatory region for the chicken EF-2 basal promoter activity.

18 for EF-2, it is demonstrated that DB blocks EF-2 binding to pre-translocative ribosome.EF-1alpha com

19 or both wild-type and D150N, suggesting that EF-2 binds constitutively to Mg(2+).

20 GTPase-activation center inhibit binding of EF-2 but not of EF-3 to yeast ribosomes.

21 EF-2 competes with EF-3 for the ribosomal binding sites

22 binding to each of the functional EF-hands (EF-2: D150N; EF-3: E186Q; and EF-4: E234Q).

23 cal/mol), EF-4 (DeltaH = +5.2 kcal/mol), and EF-2 (DeltaH = +1 kcal/mol).

24 and it was shown previously that DB prevents EF-2-dependent translocation in partial reaction models

25 In parallel with the effects on EF-2 dephosphorylation, addition of high glucose to 832/

26 The slower rephosphorylation of EF-2 during the transition from high to low glucose may

27 for protein synthesis (elongation factor-2 [EF-2], eukaryotic initiation factor-4AII, and transcript

28 When the amount of EF-2 exceeded that of ribosomes sordarin inhibited the G

29 on the ribosome-dependent GTPase activity of EF-2 from Candida albicans in the absence of any other c

30 ich specifically impair elongation factor 2 (EF-2) function.

31 eate functional domains that control chicken EF-2 gene transcription, the 5'-flanking region of the c

32 d elongation factor (EF-G in prokaryotes and EF-2 in eukaryotes).

33 F-G) in prokaryotes and elongation factor 2 (EF-2) in eukaryotes].

34 NAD to elongation factor-2 (EF-2), rendering EF-2 inactive.

35 toxin-mediated ADP-ribosylation to assay for EF-2, it is demonstrated that DB blocks EF-2 binding to

36 m high to low glucose may involve effects on EF-2 kinase activity.

37 Because EF-2 kinase is chaperoned by Hsp90, we investigated the

38 and in vivo and suggest that destruction of EF-2 kinase may be an important cytotoxic mechanism of t

39 viability of glioma cells, the expression of EF-2 kinase protein, and the interaction between Hsp90 a

40 ein phosphatase-1 nor calmodulin kinase III (EF-2 kinase) activity was affected under these condition

41 MP kinase activity in low glucose stimulates EF-2 kinase.

42 otein, and the interaction between Hsp90 and EF-2 kinase.

43 glioma cells was abrogated by overexpressing EF-2 kinase.

44 Elongation factor-2 (EF-2) kinase (calmodulin kinase III) is a unique protein

45 lines for 24-48 h of GA or 17-AAG disrupted EF-2-kinase/Hsp90 interactions as measured by coimmunopr

46 h of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49, Tyr81, V

47 We propose that Mg(2+) binding at EF-2 may structurally bridge DREAM to DNA targets and th

48 Neomycin relieves the inhibitory effect of EF-2 on EF-3 function.

49 lucose resulted in a progressive increase in EF-2 phosphorylation that was maximal by 1-2 h.

50 Histamine-stimulated EF-2 phosphorylation was not inhibited and prostacyclin

51 high glucose led to a marked stimulation of EF-2 phosphorylation, consistent with the possibility th

52 Elongation factor 2 (EF-2) plays a key role in the essential process of prote

53 8-bromo-cAMP increased chicken EF-2 promoter activity (-700/+102) in Rat 1 HIR fibrobla

54 ption, the 5'-flanking region of the chicken EF-2 promoter was analyzed.

55 provide a novel regulatory mechanism for the EF-2 promoter.

56 e basal level promoter activity as the whole EF-2 promoter.

57 This report demonstrates that chicken EF-2 protein levels are dependent on transcription in 8-

58 EF-2 regulates the outcome of protein synthesis in mamma

59 -ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive.

60 lucose promoted a rapid dephosphorylation of EF-2 that was complete in 10 min and maintained over the

61 ds NAD and catalyzes the ADP-ribosylation of EF-2, the crystal structure of DT in complex with NAD ha

62 ingly, glucose-mediated dephosphorylation of EF-2 was completely blocked by the mitochondrial respira