ライフサイエンスコーパス: EM

1 EM fungal communities were assessed with high-throughput

2 EM fungi on the root systems of both hosts were compared

3 EM has a poor prognosis during the acute phase, despite

4 EM map results (at 15-25 A resolution) indicate that bin

5 EM replica immunogold labeling, however, demonstrated on

6 EM was used to reconstruct the assembly of the full-leng

7 EM-SDA is more accurate and sparse than competing method

8 EM-SDA is tested via simulations and case studies.

9 Analysis of 3D-EM reconstructions and of thin sections revealed that ge

10 We have extended 4D EM to include liquid cells without the time resolution b

11 ce, four-dimensional electron microscopy (4D EM) has enabled observation of transient structures and

12 minex in acute and convalescent sera from 91 EM patients, in serum and synovial fluid from 141 LA pat

13 s comparable or greater sensitivity in acute EM, and similar specificity compared with conventional 2

14 Warming selectively altered EM fungal community composition at both the phylum and g

15 and corrects for sequencing bias through an EM algorithm.

16 n-maximization sparse discriminant analysis (EM-SDA), produces a sparse LDA model for datasets with a

17 inversely correlated with EM Ascomycete and EM short-contact exploration type abundance, which may r

18 warming strongly favored EM Ascomycetes and EM fungi with short-contact hyphal exploration types.

19 es with gHgL using X-ray crystallography and EM to identify their epitope locations.

20 studies, involving X-ray crystallography and EM, we show that PA41 recognizes a single, highly conser

21 Electrophysiology and EM studies uncovered that the number of multigranular co

22 EA, GQOL, and EM were restored to baseline levels during follow-up, wh

23 als (Elaris Endometriosis I and II [EM-I and EM-II]) to evaluate the effects of two doses of elagolix

24 anding the link between host performance and EM fungal community structure will to clarify how climat

25 eavy-metal staining, embedding in resin, and EM imaging.

26 mbination of X-ray crystallography, SAXS and EM, together with biochemical evidences, here we show th

27 ngs and composition of their root-associated EM fungi.

28 One such example is the need for automated EM image segmentation for neurite reconstruction.

29 ves the understanding of the linkage between EMs and MCSs.

30 d is useful for numerous applications beyond EM, including live-cell proteomic mapping.

31 By EM, such mutants induced prominent vesicle clustering, l

32 double-tetrameric population, detectable by EM.

33 he extended state of CaMKIIalpha resolved by EM is the predominant form of the holoenzyme, even under

34 to strengthen memories that are degraded by EMs.

35 a limit of detection of 3 x 10(-8) capital EM, Cyrillic.

36 Taking human annotation of cAT EM data as ground truth, we show that our algorithm dete

37 (IP) and linear programming (LP) to compute EMs in a sequential manner.

38 eved significant time reduction in computing EMs by orders of magnitude.

39 work are easily incorporated into concurrent EM efforts and broaden the application opportunities and

40 In contrast, EM remains the gold standard for reliable identification

41 In contrast, EM structures of two other E. coli MCE proteins show tha

42 Conversely, EM and TEMRA cells share a very similar epigenetic lands

43 e provide a resource comprised of correlated EM, RNAseq, and ATACseq data, showing that the growth of

44 d parameters and demonstrate how time-course EM can further dissect stoichiometric states of complexe

45 Cryo-EM analysis reveals the entry path of the primer strand

46 Cryo-EM reconstruction was used to demonstrate the structure

47 Cryo-EM reconstructions of yeast PICs suggest remodeling of t

48 Cryo-EM reveals that the dynamic ends of alpha1B/betaI+betaIV

49 In 2013, cryo-EM and x-ray structures of soluble, cleaved SOSIP Env tr

50 nsity map, analysis and annotation of a cryo-EM density map still primarily rely on fitting atomic or

51 milarly, fit of stem atoms and fit to a cryo-EM density map.

52 cedure to derive an atomic model from a cryo-EM map with annotated metadata.

53 e disassembly, we recently determined a cryo-EM reconstruction of yeast Vo The structure indicated th

54 ition to the crystal structures, a 15-A cryo-EM reconstruction reveals interdomain flexibility of the

55 We used a 3.5-A cryo-EM reconstruction with imposed D7 symmetry to further an

56 Here, we present a cryo-EM structure of a hamster SUR1/rat Kir6.2 channel bound

57 Here, we report a 6.9 A cryo-EM structure of F-actin complexed with the L253P ABD.

58 of direct electron counting to obtain a cryo-EM structure of human Ad5 at 3.2-A resolution.

59 We present a cryo-EM structure of mouse Piezo1 in a closed conformation at

60 gy, Heuer et al. (2017) present a 3.9-A cryo-EM structure of the 40S:ABCE1 post-splitting complex.

61 The 3.3-A cryo-EM structure of the 860-A-diameter isometric mutant bact

62 arameters influence the resolution of a cryo-EM structure.

63 Here we present a cryo-EM, 6.8-A resolution structure of an "immature" Chikungu

64 In addition, cryo-EM can be used to observe electron-beam induced dissipat

65 This improved resolution will allow cryo-EM to make groundbreaking contributions in essential asp

66 ysis, flexibility and hotspot analysis, cryo-EM flexible fitting, and transition pathway modeling) ba

67 tent with previous crystallographic and cryo-EM studies, the obtained force-extension curves on array

68 n fluorescent technology, genetics, and cryo-EM.

69 Comparisons between cryo-EM structures of Env trimer complexed with BG1 (6.2 A re

70 channel, NOMPC, was recently solved by cryo-EM, revealing a bundle of helices that may act as coiled

71 in its ground state by determining ClpC cryo-EM structures with and without MecA.

72 Electron cryomicroscopy (cryo-EM) has been used to determine the atomic coordinates (m

73 ent process by electron cryomicroscopy (cryo-EM) to reveal the structure of the substrate-bound inter

74 determined by electron cryomicroscopy (cryo-EM).

75 Using cutting-edge cryo-EM technology with electron counting, we improved the st

76 pEM to several challenging experimental cryo-EM datasets demonstrated its ability to avoid the select

77 aithful replication of the experimental cryo-EM map computed using the coordinates and associated met

78 Ia, VIII, and IX from conventional/film cryo-EM and X-ray crystallography studies have caused confusi

79 an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate.

80 and to visualize the detergent belt for Cryo-EM studies.

81 etion of macromolecular structures from cryo-EM density maps at 3-5-A resolution.

82 tic recognition of particle images from cryo-EM micrographs.

83 resents a major practical bottleneck in cryo-EM structural determination.

84 5-15 d for an individual experienced in cryo-EM.

85 segments can be accurately modeled into cryo-EM density maps of different resolution by FragFit.

86 ilitate modeling of macromolecules into cryo-EM density maps, fast and easy to use methods for modeli

87 ble protein parts or hinge-regions into cryo-EM density maps.

88 We determined electron cryo-microscopy (cryo-EM) and crystal structures of unbound and H1-bound nucle

89 ngle-particle cryo-electron microscopy (cryo-EM) and image classification to samples in the presence

90 ographic film cryo-electron microscopy (cryo-EM) and X-ray crystallography studies, but discrepancies

91 onstrate that cryo-electron microscopy (cryo-EM) can be used to image nanoscale lipid and polymer-sta

92 ngle-particle electron cryo-microscopy (cryo-EM) data processing allowing for the rapid determination

93 Cryo-electron microscopy (cryo-EM) had played a central role in the study of ribosome s

94 ngle-particle cryo-electron microscopy (cryo-EM) has become a mainstream tool for the structural dete

95 , progress in cryo-electron microscopy (cryo-EM) has made possible the visualization, at increasingly

96 Although electron cryo-microscopy (cryo-EM) has recently achieved resolutions of better than 3 A

97 e analysis of electron cryo-microscopy (cryo-EM) is a key technology for elucidation of macromolecula

98 ngle-particle electron cryo-microscopy (cryo-EM) is an emerging tool for resolving structures of conf

99 ructures into cryo-electron microscopy (cryo-EM) maps is a major challenge, as the moderate resolutio

100 gh resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of natu

101 on (3.9-4.2A) cryo-electron microscopy (cryo-EM) reconstructions of MTs stabilized by the taxane-site

102 In addition, cryoelectron microscopy (cryo-EM) reconstructions of virion capsids did not detect any

103 Combining cryo-electron microscopy (cryo-EM) structure analysis and biochemical approaches, we de

104 ort the 4.2-A cryo-electron microscopy (cryo-EM) structure and in vitro dynamics parameters of alpha1

105 e present the cryo-electron microscopy (cryo-EM) structure of a full-length TRPML3 channel from the c

106 we present the cryoelectron microscopy (cryo-EM) structure of a KCNQ1/calmodulin (CaM) complex.

107 gh-resolution cryo-electron microscopy (cryo-EM) structure of the core tetrameric HIV-1 STC and a hig

108 we report the cryo-electron microscopy (cryo-EM) structure of the Csy complex bound to two different

109 s analyzed by cryo-electron microscopy (cryo-EM) to date.

110 ted cryo-fLM, cryo-electron microscopy (cryo-EM), and cryo-ET (i.e., cryo-CLEM) of virus-infected or

111 We report cryo-electron microscopy (cryo-EM), biophysical, biochemical, and cell biological studi

112 ngle-particle cryo-electron microscopy (cryo-EM), molecules suspended in a thin aqueous layer are rap

113 ding studies, cryo-electron microscopy (cryo-EM), mutational analyses, peptide binding analysis, link

114 ngle-particle cryo-electron microscopy (cryo-EM), reveal structural details that help explain the wid

115 rom ssNMR and cryo-electron microscopy (cryo-EM), we establish an atomic resolution model of the RSV

116 esolutions by cryo-electron microscopy (cryo-EM).

117 mined by cryogenic electron microscopy (cryo-EM).

118 el of Hsp21 based on homology modeling, cryo-EM, cross-linking mass spectrometry, NMR, and small-angl

119 By integrating NMR, cryo-EM, and molecular dynamics simulations, we show that in

120 single-particle recognition from noisy cryo-EM micrographs, enabling automated particle picking, sel

121 gnificantly improving the efficiency of cryo-EM data processing.

122 The procedure optimizes contrast of cryo-EM densities by amplitude scaling against the radially a

123 ignificantly enhanced the resolution of cryo-EM density maps and broadened the applicability and the

124 neral procedure for local sharpening of cryo-EM density maps based on prior knowledge of an atomic re

125 This study demonstrates the utility of cryo-EM in revealing structure dynamics within a single prote

126 has greatly improved the resolution of cryo-EM structures to the point where atomic resolution is no

127 specimen orientation in single particle cryo-EM and present open-source software for rapidly assessin

128 rms, were determined by single-particle cryo-EM at 3.9 A and 5.6 A, respectively.

129 re, we describe a 3.6 A single-particle cryo-EM reconstruction of the core CBF3 complex, incorporatin

130 esent the CVA6 procapsid and A-particle cryo-EM structures and identify an immune-dominant neutralizi

131 SpoIIIAG, determined by single-particle cryo-EM.

132 Here, we present cryo-EM structures of Drp1 helices on nanotubes with distinct

133 We present cryo-EM structures of E. coli RNAP core bound to the small ri

134 Here we present cryo-EM structures of the unique minus-end directed myosin VI

135 ure unambiguously confirms our previous cryo-EM models of proteins IIIa, VIII, and IX and explains th

136 automated particle extraction from raw cryo-EM micrographs in the absence of a template.

137 A recent cryo-EM structure of a native full-length trimer without any

138 Recent cryo-EM structures of the Mcm2-7 (MCM) double hexamer, its pr

139 A recent cryo-EM study of holo V-ATPase revealed three major conformat

140 Until recently cryo-EM structures were limited to approximately 10 A in the

141 Here, we report cryo-EM structures of an AMPAR in complex with the auxiliary

142 We provide a high-resolution cryo-EM structure of a virus-ICAM-1 complex, which revealed c

143 We report a 4.3 A resolution cryo-EM structure of the active Vps4 hexamer with its cofacto

144 lding upon our earlier 4.3 A resolution cryo-EM structure, we now report a 3.2 A structure of Vps4 bo

145 We report two 3.2 A resolution cryo-EM structures - determined from a single sample - of the

146 Here we present near-atomic resolution cryo-EM structures for flagellar filaments from both Gram-pos

147 authors present near-atomic resolution cryo-EM structures of nine flagellar filaments, and begin to

148 By testing the procedure using six cryo-EM structures of TRPV1, beta-galactosidase, gamma-secret

149 ture agrees well with a recently solved cryo-EM structure of a CFTR IWF state.

150 hway modeling) based on an active-state cryo-EM map.

151 These findings demonstrate that cryo-EM allows atomic characterization of amyloid filaments f

152 w, we summarize important insights that cryo-EM, in combination with chemical and genetic approaches,

153 on the orientation of capsomers in the cryo-EM reconstruction, we propose that the capsids of CroV a

154 Analysis of the cryo-EM spliceosome B(act) complex shows that the resistance

155 ity maps are the ultimate result of the cryo-EM structure determination process.

156 Here, Wang et al. report the cryo-EM structure of mature JEV at near-atomic resolution and

157 We present the Cryo-EM structure of Saccharomyces cerevsisae Tra1 to 3.7 A r

158 Here, we present the cryo-EM structure of the entire SAGA complex where the major

159 f the mature subunit, we determined the cryo-EM structure of the fully assembled 30S subunit in compl

160 fold, which is largely invisible in the cryo-EM structure of the HKU1 S trimer.

161 Here, we report the cryo-EM structure of the Mcm2-7 DH on dsDNA and show that the

162 Here, we present the cryo-EM structure of this bifunctional complex at a resolutio

163 activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodified or pho

164 Here we present two cryo-EM snapshots of the Thermobifida fusca type I-E Cascade:

165 Here, we used cryo-EM to elucidate structural basis of channel assembly and

166 We used cryo-EM to study a heterogeneous population of SBPV virions a

167 Using cryo-EM, we show that the membrane in AFV1 is a 2 nm-thick m

168 combining hybrid mass spectrometry with cryo-EM, computational and biochemical data, we investigate t

169 xocytotic defects caused by Syn-1A deletion, EM and total internal reflection fluorescence microscopy

170 r memory (EM), and terminally differentiated EM (TEMRA) cells.

171 ction deletion constraint becomes a distinct EM.

172 The most dominant EM fungal taxon detected in the homogeneous treatment wa

173 termetallic compound/solder interface during EM testing.

174 ymbiosis between plants and ectomycorrhizal (EM) fungi.

175 o quantify the responses of ectomycorrhizal (EM) fungal communities associated with poorly performing

176 872 women underwent randomization in Elaris EM-I and 817 in Elaris EM-II; of these women, 653 (74.9%

177 In Elaris EM-I, the percentage of women who had a clinical respons

178 In Elaris EM-I, the percentage of women who had a clinical respons

179 d with 19.6% in the placebo group; in Elaris EM-II, the corresponding percentages were 43.4% and 72.4

180 oup (P<0.001 for all comparisons); in Elaris EM-II, the corresponding percentages were 49.8% and 57.8

181 ndomization in Elaris EM-I and 817 in Elaris EM-II; of these women, 653 (74.9%) and 632 (77.4%), resp

182 equires the detection of an electromagnetic (EM) counterpart.

183 nhanced due to strong local electromagnetic (EM) fields.

184 is an important property of electromagnetic (EM) wave and different polarization manipulations are re

185 ra-wideband manipulation of electromagnetic (EM) waves.

186 ectrostatic effect on the electromechanical (EM) response in piezoresponse force microscopy as a mode

187 Cu and SN100C solders were electromigration (EM) tested, and the 3D laminography imaging technique wa

188 r cells, is responsible for electromotility (EM) and a corresponding nonlinear capacitance (NLC).

189 cells and fibroblasts from the endometrium (EM), endocervix (CX) and ectocervix (ECX) significantly

190 trongly resonant with the antenna's enhanced EM fields.

191 Notably, warming strongly favored EM Ascomycetes and EM fungi with short-contact hyphal ex

192 m wavelength range, show that the near-field EM radiation can be extracted to the far-field by establ

193 e-saturation fusion method often applied for EM-sharpening can result in serious image artifacts, esp

194 tually, for obtaining the electrostatic-free EM response.

195 roadly applicable genetic tag that generates EM contrast on a specific protein or subcellular compart

196 of which disables all previously identified EMs.

197 ase 3 trials (Elaris Endometriosis I and II [EM-I and EM-II]) to evaluate the effects of two doses of

198 mer and selectively enhances its contrast in EM.

199 ficant extent by a corresponding increase in EM in multivalent complexes.

200 re correlated with their volumes measured in EM (r = 0.93).

201 pating in the 3D segmentation of neurites in EM images (SNEMI3D) challenge.

202 the electron dense nanoparticle observed in EM.

203 The transcriptional pattern in EM biopsies consisted of 254 differentially regulated ge

204 Given the variation in EM host responses to warming, both within and between ec

205 provides mixed information of both the local EM field strength and the local density of states.

206 and a variational expectation maximization (EM) algorithm to estimate non-reference allele frequency

207 n coupled with the Expectation-Maximization (EM) algorithm, reads can be assigned far more accurately

208 and their related expectation-maximization (EM) algorithms.

209 Experimental medicine (EM) offers an approach that can help investigators speci

210 pression profiles in naive, effector memory (EM), and terminally differentiated EM (TEMRA) cells.

211 ed bulk flow through 3D electron microscope (EM) reconstructions of interstitial space.

212 scent labeling, and 3D electron microscopic (EM) reconstruction of rat CA3 pyramidal cell axon termin

213 Quantitative electron microscopy (EM) analysis revealed age-dependent increases of autopha

214 orrelative 2PLSM and 3D electron microscopy (EM) analysis, performed in mouse barrel cortex, showed t

215 nal circuits, including electron microscopy (EM) and light microscopy (LM).

216 using serial block-face electron microscopy (EM) and RNA sequencing.

217 lso be used for (cryo-) electron microscopy (EM) and small-angle X-ray and neutron-scattering studies

218 On virions, electron microscopy (EM) and tomography reveal monomeric spikes similar to on

219 Progress in 3D electron microscopy (EM) imaging has greatly facilitated neuroscience researc

220 Electron microscopy (EM) is the premiere technique for high-resolution imagin

221 decade, high resolution electron microscopy (EM) of serial sections has become the de-facto standard

222 nce microscopy (FM) and electron microscopy (EM) offer complementary advantages and disadvantages for

223 Single-particle electron microscopy (EM) revealed nuclear PER complexes purified from mouse l

224 lysis by negative-stain electron microscopy (EM) reveals an ensemble of dormant AKAP79-PP2B configura

225 in vitro structures and electron microscopy (EM) studies, the hierarchical model is that 11-nanometer

226 we used single particle electron microscopy (EM) to determine a pseudoatomic model of the CaMKIIalpha

227 -ray scattering (SAXS), electron microscopy (EM), and biophysical analysis confirmed agreement of the

228 y traditionally employs electron microscopy (EM), but this platform has significant limitations inclu

229 vesicles and immunogold electron microscopy (EM), we characterize the existence of LVPs that cofracti

230 rom crystallography and electron microscopy (EM), we show that GINS is a compact tetramer in solution

231 l images obtained using electron microscopy (EM).

232 responses in patients with erythema migrans (EM) or Lyme arthritis (LA) to elucidate their role early

233 early Lyme disease is the erythema migrans (EM) skin lesion that develops at the tick bite site typi

234 sensitive in patients with erythema migrans (EM), the most common manifestation of the illness.

235 Ensemble modeling (EM) was developed to circumnavigate this challenge and e

236 were used to determine effective molarities (EM) for the formation of intramolecular phenol-amide H-b

237 MDR, patients make horizontal eye movements (EMs) while simultaneously recalling a traumatic memory,

238 Eosinophilic myocarditis (EM) is an acute life-threatening inflammatory disease of

239 hila's motion-sensing T4 cells using a novel EM technique.

240 anuscripts in MEDLINE and EMBASE on cases of EM published until June 2017.

241 st pine seedlings, reducing the diversity of EM fungi at the treatment level, while substantially imp

242 24 dB between two orthogonal orientations of EM wave polarization for incidence angles in the range o

243 ical presentation, treatment, and outcome of EM.

244 tional alterations in skin biopsy samples of EM lesions from untreated adult patients with Lyme disea

245 izing ultra-wideband diffusion scattering of EM wave, which is important for stealth and other microw

246 The values of EM increase by an order of magnitude with increasing con

247 There were no effects of EMs on memory emotionality or psychophysiological measur

248 ould abolish the memory degrading effects of EMs.

249 hallenging due to combinatorial explosion of EMs in complex networks.

250 Identification of the full set of EMs in genome-scale networks remains challenging due to

251 Mammalian-derived cells are cultured on EM substrates, using optimized conditions that ensure th

252 nus levels, but had no significant effect on EM fungal operational taxonomic unit (OTU) diversity.

253 Although this protocol focuses on EM in cultured mammalian cells, APEX2 is applicable to m

254 Furthermore, single-particle EM analysis revealed, for the first time, the overall ar

255 rovide proof of concept that single-particle EM can be used to generate a "visual" kinetic profile th

256 Here, we show how single-particle EM can enrich our understanding of protein-protein inter

257 Single-particle EM of two purified cytoplasmic PER complexes revealed ap

258 e, we conducted a systematic single-particle EM study on multiple permutations of the reconstituted C

259 Vo into lipid nanodiscs for single-particle EM.

260 ditions using negative stain single-particle EM.

261 LLR, a novel expectation-maximization-path (EM-path) algorithm has been developed to greatly reduce

262 tigue (FA; QLQ-C30), and emotional problems (EM; QLQ-OES24).

263 itted to hospital with histologically proven EM.

264 etest to posttest and follow-up after recall+EMs relative to the control conditions.

265 ver, memories become less vivid after recall+EMs.

266 owever, that only a small subset of relevant EMs needs to be known, for which optimization-based sequ

267 e structure was fitted into an existing SAGA EM reconstruction and reveals limited contact surfaces t

268 ural connectivity at ever increasing scales (EM connectomics).

269 ion of paired patch-clamp recordings, serial EM, and large-scale multi-electrode array recordings to

270 In the simulations, EM-SDA is compared with nearest shrunken centroids (NSCs

271 culum was homogenously distributed, a single EM fungal taxon dominated the roots of most pine seedlin

272 PXRD, solid-state spectroscopy, EM analysis, and quantum-chemical calculations suggest a

273 crylamide gel shift assay and negative-stain EM, we found that the prefusion conformational state of

274 tional steps required relative to a standard EM sample preparation are cell transfection and a 2- to

275 Compared with healthy subjects, EM patients had significantly higher levels of innate, T

276 The EM approach promises progress in answering the key quest

277 fore, directly after, and 24 hours after the EM task.

278 guidelines for quantitatively analyzing the EM response as well as, eventually, for obtaining the el

279 r, volume, and growth rate of voids, and the EM parameter of DZ*.

280 sting largely on correlational evidence, the EM approach emphasizes experimental tests of targets or

281 Furthermore, the EM-SDA results are mostly consistent between the missing

282 tion procedure, since each round of e.g. the EM algorithm, can execute much more quickly.

283 only offers a computational advantage in the EM analysis of genome-scale networks, but also improves

284 que provides an effective way of mapping the EM field or the local density of states with nanometre s

285 Nuclear Spectroscopic Telescope Array of the EM counterpart of the binary neutron star merger GW17081

286 Analyses of the EM structures provided information relevant to vaccine d

287 frequencies of gametes and outperformed the EM algorithm in estimating recombination fractions betwe

288 f two-point recombination fractions than the EM algorithm.

289 These EM studies also demonstrate that the complex exhibits co

290 Our complementary improvements to this EM framework are easily incorporated into concurrent EM

291 d evaluate this method both by comparison to EM gold standard annotated data and by examining its cap

292 the thioflavin T binding assay, transmission EM, and molecular dynamics simulations, here we identifi

293 Unfortunately, EM, in its base form, requires long solve times to compl

294 We used EM and crosslinking mass spectrometry to dissect five co

295 We developed a variational EM algorithm for a hierarchical Bayesian model to identi

296 e, we show that our model with a variational EM inference algorithm has higher specificity than many

297 We demonstrate that our variational EM algorithm has comparable sensitivity and specificity

298 nfocal and super-resolution FM images, where EM cross-validation is not practical.

299 he disorders most frequently associated with EM were hypersensitivity and eosinophilic granulomatosis

300 also significantly inversely correlated with EM Ascomycete and EM short-contact exploration type abun