ライフサイエンスコーパス: ER-beta

1 ER-beta can play an important role in CRC development an

2 ER-beta mRNA was significantly up-regulated in the tamox

3 ER-beta only modestly affected FR-alpha promoter activit

4 ER-beta variants were also detected.

5 ER-beta/PV double-labeled cells are also observed within

6 ER-beta/PV double-labeled cells represent 23.3% +/- 1.6%

7 of these studies have demonstrated that (1) ER beta mRNA is translated into immunoreactive protein t

8 ve protein throughout the rat brain, and (2) ER beta resides in the cell nucleus.

9 these studies indicate that ligand-activated ER-beta is a potential therapeutic target to combat obes

10 Almost all ER-beta-immunoreactive cells within the subiculum, a maj

11 .8% (intact) and 94.5% +/- 1.4% (ovx) of all ER-beta-immunoreactive cells coexpress parvalbumin, and

12 ive brain area reported to express ER alpha, ER beta, and PR.

13 a and PR, and PC-3 cells exhibited ER-alpha, ER-beta, and pS2 mRNA.

14 the region-specific expression of ER-alpha, ER-beta, or both may be important in determining the phy

15 ition of the four genes coding for ER-alpha, ER-beta, OT, and the OT receptor.

16 fter the raloxifene treatment, PC3 (ER-alpha/ER-beta+) and DU145 (ER-beta+ only) cells were selected

17 vasopressin-immunoreactive neurons were also ER-beta positive in the PVN (66.14% +/- 2.47%) and SON (

18 An ER-beta-selective ligand increased markers of tricarboxy

19 (i) ER-beta1 is the obligatory partner of an ER-beta dimer, whereas the other isoforms function as va

20 versed by cotreatment of DU145 cells with an ER-beta antisense oligonucleotide, hence lending additio

21 gues can activate ER alpha(Glu353-->Ala) and ER beta(Glu305-->Ala) with very large selectivites, demo

22 , whereas CAV3 is necessary for ER alpha and ER beta activation of mGluR2/3.

23 ER alpha and ER beta agonists PPT and DPN inhibited and 4-OHT increas

24 Both ER alpha and ER beta contribute to E2-mediated EPC activation and tis

25 elped to delineate the roles of ER alpha and ER beta in modulating transcription of genes containing

26 discriminate between different ER alpha and ER beta ligand complexes suggests that the biological ef

27 , in weanling rats sex affected ER alpha and ER beta neuronal densities in brainstem regions associat

28 factor requirements, domains of ER alpha and ER beta sufficient for forskolin/IBMX activation, and th

29 n, we compared the abilities of ER alpha and ER beta to activate transcription and induce distortion

30 The different abilities of ER alpha and ER beta to induce change in DNA structure could foster o

31 istinct mechanisms to stimulate ER alpha and ER beta transcriptional activity.

32 ivators stimulated cAMP-induced ER alpha and ER beta transcriptional activity.

33 , those tumors that coexpressed ER alpha and ER beta were node positive (P = 0.02; Fisher's exact tes

34 sion of the estrogen receptors (ER alpha and ER beta) on the globoid cells, activated astrocytes and

35 ng the biological roles of both ER alpha and ER beta, and they might form the basis for the developme

36 estrogen receptor (ER) subtype, ER alpha and ER beta, in the injured brain.

37 ains (E domains) of human/mouse ER alpha and ER beta, progesterone receptors A and B, and the androge

38 antitate areas and densities of ER alpha and ER beta-positive neurons within medullary regions associ

39 nscriptional activities of both ER alpha and ER beta.

40 on through the two ER subtypes, ER alpha and ER beta.

41 at ADA3 directly interacts with ER alpha and ER beta.

42 with estrogen receptor alpha (ER alpha) and ER beta bound to a variety of ligands.

43 xpression of ADA3 enhances the ER alpha- and ER beta-mediated sequence-specific transactivation.

44 we used ER alpha-knockout (ER alpha KO) and ER beta-knockout (ER beta KO) mice in an animal model of

45 inhibitory activities and high ER-alpha and ER-beta binding affinities of several of the resulting a

46 R and Western blot analysis for ER-alpha and ER-beta demonstrated that all three cell lines express E

47 henotype in mice that lack both ER-alpha and ER-beta genes (alphabetaERKO).

48 to investigate the presence of ER-alpha and ER-beta in normal and malignant colon tissue.

49 tudies revealed the presence of ER-alpha and ER-beta mRNA throughout the rostral-caudal extent of the

50 ation about the distribution of ER-alpha and ER-beta mRNAs in the rat CNS.

51 l increased the density of both ER-alpha and ER-beta protein clusters along dendrites.

52 ice had decreased expression of ER-alpha and ER-beta subtypes and ER transcriptional activity was als

53 pression of estrogen receptor (ER)-alpha and ER-beta subtypes in GS-prone and GS-resistant glomeruli

54 and BPH-1 cells expressed both ER-alpha and ER-beta transcripts and no PR nor pS2 mRNA in PrEC and o

55 Expression levels of ER-alpha and ER-beta transcripts were related to those of two estroge

56 ncreases in estrogen receptors (ER-alpha and ER-beta) and a decrease in progesterone receptor levels,

57 X is functionally distinct from ER-alpha and ER-beta, and that, like ER-alpha, it is re-expressed in

58 in the ligand-binding domain of ER-alpha and ER-beta, we were not surprised to find that SP500263 bin

59 ural estrogen genistein at both ER-alpha and ER-beta, whereas AIB1 had no effect on either the potenc

60 GGTCAnnnTGACC, which bind both ER-alpha and ER-beta.

61 xpress both estrogen receptor (ER)-alpha and ER-beta.

62 Estrogen receptor-alpha (ER-alpha) and ER-beta exhibit fine differences in their distributions

63 both estrogen receptor-alpha (ER-alpha) and ER-beta in ovarian, breast and endometrial cancer cell l

64 ssociated with reduced risk of ER-alpha- and ER-beta-expressing NSCLC.

65 ther with the inability of the ER-alpha- and ER-beta-selective ligands to elicit ERK phosphorylation,

66 17-diol (ICI 182,780)] and the ER-alpha- and ER-beta-specific agonists [1,3,5-tris(4-hydroxyphenyl)-4

67 abolish receptor binding, and ER-alpha- and ER-beta-specific antibodies interact with complexes form

68 n functions signaled through hER-alpha66 and ER-beta; it also transduces membrane-initiated estrogen-

69 nd the number of cortical GABA, ER-beta, and ER-beta/GABA double-labeled neurons was examined.

70 utions of ER-alpha immunoreactivity (ir) and ER-beta ir were nonoverlapping in the PVN and SON.

71 Expression of FOS-IR in MNCs with attenuated ER-beta-IR, and the absence of FOS-IR in parvocellular n

72 Because ER-beta is thought to function as an inhibitor of prosta

73 ta expression was selective for MNCs because ER-beta-IR remained unaltered in PVN parvocellular neuro

74 strogen receptors alpha (ER alpha) and beta (ER beta) in EPC biology are largely unknown.

75 lar receptors, alpha (ER alpha) and/or beta (ER beta), and the progestin receptor (PR).

76 The discovery of estrogen receptor beta (ER beta) and subsequent localization of its mRNA in the

77 or alpha (ER alpha), estrogen receptor beta (ER beta), progesterone receptor (PR), and androgen recep

78 etermine the role of estrogen receptor beta (ER-beta) and its ligands on adipose biology.

79 Estrogen receptor beta (ER-beta) and its selective ligand reprogrammed preadipoc

80 Estrogen receptor beta (ER-beta) regulates diverse physiological functions in th

81 alpha (ER-alpha) and estrogen receptor beta (ER-beta), and antagonize the activity of beta-estradiol

82 nd binding domain of estrogen receptor beta (ER-beta).

83 cently characterized estrogen receptor-beta (ER-beta) has not been examined in vivo.

84 at the activation of estrogen receptor-beta (ER-beta) plays an important cardioprotective role agains

85 s proliferation via oestrogen receptor-beta (ER-beta), the catecholoestradiols mediate P-UAEC prolife

86 its specificity for estrogen receptor-beta (ER-beta), was used to immunolocalize the receptor in his

87 lpha (ER-alpha), and estrogen receptor-beta (ER-beta), we found only sparse colocalization between ER

88 ctivation of estrogen receptor subtype beta (ER-beta).

89 Comparisons were made between ER-beta, estrogen receptor-alpha (ER-alpha), and androge

90 dzein and genistein (compounds known to bind ER-beta) were performed to serve as positive controls.

91 nteracting with ER alpha, ERAP140 also binds ER beta, TR beta, PPAR gamma, and RAR alpha.

92 P-dependent activation of gene expression by ER beta but not ER alpha, indicating that the former sub

93 The inhibition of anoikis by ER-beta-catenin can be abolished by a mitogen-activated

94 and anticancer effects that are mediated by ER-beta.

95 780 blocks activation by ER-alpha but not by ER-beta.

96 ditional support to a central role played by ER-beta in mediating growth-inhibitory action of antiest

97 We hypothesize that estrogen protects by ER-beta activation, which leads to S-nitrosylation (SNO)

98 This phenotype was partially reversed by ER-beta-selective ligand.

99 ER-beta-specific antibodies to characterize ER-beta protein expression in breast cancer cell lines a

100 ptor (ER) alpha and the more recently cloned ER-beta.

101 ent of both ER-alpha and the recently cloned ER-beta.

102 entage of oxytocin (OXY) neurons coexpressed ER-beta in the PVN (84.39% +/- 2.99%), there was very li

103 n erythroleukemia (HEL) cells also contained ER beta and AR transcripts.

104 ling and discovered that axons from cortical ER-beta-expressing inhibitory neurons terminate on BDNF-

105 Given the recent finding that cortical ER-beta is almost exclusively localized to parvalbumin-i

106 he RNA expression studies, we have developed ER-beta-specific antibodies to characterize ER-beta prot

107 Regardless of these differences, ER beta-mediated regulation of GST-Pi and GCSh point tow

108 reatment, PC3 (ER-alpha/ER-beta+) and DU145 (ER-beta+ only) cells were selected to further characteri

109 (ER) until the recent cloning of a novel ER (ER-beta).

110 cloning of a second estrogen receptor (ER), ER beta, has prompted a reevaluation of the role of ERs

111 Based on our recent finding of anti-estrogen/ER-beta-mediated growth inhibition of prostate cancer ce

112 cord, and pineal gland contained exclusively ER-beta mRNA.

113 s been demonstrated that LNCaP cells express ER-beta but not ER-alpha and that tamoxifene induces apo

114 monstrated that all three cell lines express ER-beta, whereas only PC3 and PC3M cells were positive f

115 oximately 7.5% of oxytocin (OT) MNCs express ER-beta.

116 pin-releasing hormone neurons also expressed ER-beta ir in the PVN (12.57% +/- 1.99%), but there was

117 prostate cancer cell lines, LNCaP expressed ER-beta mRNA along with transcripts of PR and pS2, DU145

118 howed variation in the size of the expressed ER-beta protein.

119 , with 22% of samples exclusively expressing ER beta; this was not observed in any of the breast tumo

120 urons with the highest percentage expressing ER-beta was found to be prolactin (PRL) immunoreactive i

121 tients with 5-FU-resistant tumors expressing ER-beta protein.

122 tase], "estrogen metabolism/ER-beta factor" (ER-beta, peroxisome proliferator-activated receptor-gamm

123 l human CD34(+) stem cells contained RNA for ER beta and AR, which increased with cell differentiatio

124 and GCSh point towards an important role for ER beta in cellular protection against oxidative stress.

125 contained cells that were immunostained for ER-beta.

126 sections were double- or triple-labeled for ER-beta, GABAergic, and BDNF immunomarkers.

127 ber of perirhinal neurons double-labeled for ER-beta/GABA was reduced by 28% (P<0.01 compared to vehi

128 Numerous MNCs were positive for ER-beta in control animals, but they were virtually devo

129 e results lend further support to a role for ER-beta as a poor prognostic factor in breast cancer.

130 These data highlight a new role for ER-beta in adipose biology and its potential to be a saf

131 elease, this suggests an inhibitory role for ER-beta in MNCs.

132 with beta-LGNDs demonstrated selectivity for ER-beta over ER-alpha.

133 anslationally modified form of the long-form ER-beta, which has a predicted size of 59 kd based on po

134 Furthermore, ER-beta and BDNF do not colocalize in any brain region.

135 replacement and the number of cortical GABA, ER-beta, and ER-beta/GABA double-labeled neurons was exa

136 In general, ER beta-positive neurons in the brainstem regions examin

137 Silencing of hippocampal ER-beta attenuated E2-mediated ischemic protection sugge

138 ory of both ERs and discuss the implications ER beta has to patients with breast cancer.

139 In contrast sex differences in ER beta were found in fewer nuclei, but in those higher

140 or propyl pyrazole triol in wild-type and in ER-beta-/- mice.

141 The osmotically induced decrease in ER-beta expression was selective for MNCs because ER-bet

142 ochemistry demonstrated that the decrease in ER-beta mRNA was translated into depletion of receptor p

143 transcription-PCR revealed no difference in ER-beta mRNA levels between normal and malignant colon t

144 cardioprotective effect of DPN was found in ER-beta-knockout mice, indicating that the DPN-induced c

145 ivo findings that methylation is involved in ER-beta silencing.

146 fects of ER-beta ligand were not observed in ER-beta-knockout mice.

147 ia d-d-arginine VP and liquid diet increased ER-beta mRNA expression in MNCs (p < 0.05).

148 The AD-like effect of estradiol involved ER beta and G-protein coupled receptor 30, whereas its b

149 knockout (ER alpha KO) and ER beta-knockout (ER beta KO) mice in an animal model of stroke.

150 We ovariectomized ER alpha KO, ER beta KO, and the respective wild-type mice and implan

151 adiol differ dramatically among ER alpha KO, ER beta KO, and wild-type mice.

152 sistent with the 530-amino acid, full-length ER-beta sequence.

153 lines (LNCaP and DU145), that express little ER-beta mRNA, with a demethylating agent increased level

154 VN (84.39% +/- 2.99%), there was very little ER-beta/OXY colocalization in the SON.

155 ting the first 18 amino acids of the longest ER-beta open reading frame reported to date, and polyclo

156 ndometrium (4.9) mainly as a result of lower ER-beta expression in the former.

157 profiling; however, the estrogen metabolism/ER-beta factor seemed to be distinctive.

158 lin D1, and aromatase], "estrogen metabolism/ER-beta factor" (ER-beta, peroxisome proliferator-activa

159 , and in contrast to the estrogen metabolism/ER-beta factor, higher current body mass index among pre

160 d developmentally regulated, and neocortical ER-beta, which is intranuclear and expressed throughout

161 The present study used a new ER beta-specific polyclonal antiserum (Z8P) and immunocy

162 the PVN (12.57% +/- 1.99%), but there was no ER-beta colocalization with TRH.

163 he developing brain, is neither ER-alpha nor ER-beta but a novel, plasma membrane-associated, putativ

164 estrogen receptor-alpha (ER alpha), but not ER beta, inhibited estrogen-stimulated telomerase functi

165 will require antagonism of ER alpha but not ER beta.

166 pressed estrogen receptor (ER)alpha, but not ER-beta protein levels, and abrogated downstream estroge

167 A and protein expression of ER-alpha but not ER-beta were suppressed by resveratrol in Ishikawa cells

168 or an estrogen receptor (ER)-alpha (but not ER-beta) agonist into the dorsal hippocampus rapidly imp

169 s were intact in male mice lacking the novel ER-beta gene.

170 ution of the classical (ER-alpha) and novel (ER-beta) forms of ER mRNA-expressing neurons in the cent

171 ted with cytoplasmic ER-alpha and/or nuclear ER-beta expression-defined NSCLC in postmenopausal women

172 njury is totally preserved in the absence of ER beta.

173 try (ICC) to investigate the distribution of ER beta in the rat CNS.

174 In normal breast tissue, expression of ER beta predominated, with 22% of samples exclusively ex

175 g through the AP1 element, overexpression of ER beta in tumors expressing both ER subtypes may explai

176 ern immunoblotting confirmed the presence of ER beta and AR in platelets.

177 vity by hPMC2 is enhanced in the presence of ER beta.

178 osphorylation of the corresponding region of ER beta, and this correlates with the lack of forskolin/

179 A critical step in understanding the role of ER beta is demonstrating that the mRNA is translated int

180 Targeting of ER beta or G-protein coupled receptor 30 might reveal a

181 avourable disease outcome, the usefulness of ER beta as a clinical prognostic marker remains to be de

182 used to determine the mechanism of action of ER-beta and its ligands.

183 re protects hearts largely via activation of ER-beta and nitric oxide/SNO signaling.

184 The activation of ER-beta by DPN treatment leads to increased protein SNO

185 The activation of ER-beta-catenin signaling rescues RK3E cells from anoiki

186 oprotection occurs through the activation of ER-beta.

187 for studying ER-beta signaling and design of ER-beta-based therapies.

188 l animals, but they were virtually devoid of ER-beta-immunoreactivity (IR) in hyper-osmotic animals.

189 on is associated with a marked diminution of ER-beta protein expression, possibly through a posttrans

190 tions requiring the ligand binding domain of ER-beta and through abrogation of the ability of PGC-1 t

191 We evaluated the pharmacological effect of ER-beta-selective ligands (beta-LGNDs) in animal models

192 The antiobesity effects of ER-beta ligand were not observed in ER-beta-knockout mic

193 Expression of ER-beta in metastases may have been influenced by the lo

194 Thus, the expression of ER-beta mRNA correlated inversely with changes in plasma

195 se relationship exists between the extent of ER-beta CGI methylation and receptor expression in norma

196 The importance of ER-beta in tamoxifen resistance was validated using tamo

197 CGIs, led to transcriptional inactivation of ER-beta.

198 hat antiestrogens antagonize E2 induction of ER-beta mRNA.

199 ecause the prostate contains a high level of ER-beta, the present study investigated the effect of ra

200 Because the prostate contains high levels of ER-beta, the present study investigated the effect of ra

201 arcinogenesis was characterized by a loss of ER-beta expression at the protein and transcript levels

202 promoter CGI, which correlated with loss of ER-beta expression, was detected in microdissected sampl

203 nant colon tissue showed a selective loss of ER-beta protein expression when compared to normal colon

204 post-ischemic hippocampal injury by means of ER-beta activation.

205 s of PR and pS2, DU145 expressed messages of ER-beta and PR, and PC-3 cells exhibited ER-alpha, ER-be

206 Thus, osmotic modulation of ER-beta expression in MNCs may augment or attenuate an i

207 - 3.1% (intact) and 26.0% +/- 5.2 % (ovx) of ER-beta-immunoreactive cells coexpress PV.

208 try to further characterize the phenotype of ER-beta-bearing cells by double labeling for the GABAerg

209 However, the potential of ER-beta-selective ligands to offset obesity is not clear

210 state cancer cells in vitro, the presence of ER-beta in metastatic cells may have important implicati

211 We find that a large proportion of ER-beta-immunoreactive cells within the cortex, amygdala

212 ranslated exon 0N and the promoter region of ER-beta.

213 for neuronal activation in the regulation of ER-beta expression in MNCs.

214 first evidence that epigenetic regulation of ER-beta is a reversible and tumor stage-specific process

215 We examined the osmotic regulation of ER-beta mRNA expression in MNCs using quantitative in si

216 of estrogen receptor (ER)-alpha and those of ER-beta were expressed in our normal PrEC primary cultur

217 Interestingly, only transcripts of ER-beta, but not those of ER-alpha, were found in our pr

218 tection assay a mRNA coding for a variant of ER-beta that is coexpressed with wild-type ER-beta in th

219 activity on ER alpha while being inactive on ER beta.

220 t binds to both receptors, but enhances only ER beta interaction with SRC1 and SRC3 while exhibiting

221 contrast, in DU145 cells, which express only ER-beta, antiestrogens, but not estrogens, are growth in

222 2-mediated responses were due to ER alpha or ER beta signaling, ER alpha-knockout (alphaERKO) or ERbe

223 for one of the two ER subtypes, ER alpha or ER beta.

224 ense riboprobes complimentary to ER-alpha or ER-beta mRNA, stringently washed, and opposed to emulsio

225 In cells containing either ER-alpha or ER-beta, the 3'-element behaves as a traditional enhance

226 es were associated with nuclear ER-alpha- or ER-beta-negative NSCLC.

227 sk of NSCLC characterized as ER-alpha and/or ER-beta positive.

228 expression plasmid pCI-ER alpha, but not pCI-ER beta, aromatase activity was elevated by 17beta-estra

229 cates the involvement of another ER, perhaps ER beta.

230 Additionally, periodic ER-beta agonist treatments every 48 hr improved post-isc

231 gene for a likely gene duplication product, ER-beta, did not have these effects.

232 In normal prostate, ER-beta immunostaining was predominately localized in th

233 Recently, ER-beta (ERbeta) mRNA and protein were shown to be expre

234 cently discovered estrogen-binding receptor, ER beta.

235 abundant cortical nuclear estrogen receptor, ER-beta, is present in GABAergic neurons, prompting us t

236 FOS-IR in parvocellular neurons that retain ER-beta-IR suggest a role for neuronal activation in the

237 Shorter ER-beta isoforms were detected in the ER-alpha-negative

238 viously unrecognized directions for studying ER-beta signaling and design of ER-beta-based therapies.

239 e after trauma-hemorrhage and female 129 Sve ER-beta-/- transgenic mice and ovariectomized wild-type

240 s containing the consensus ERE sequence than ER beta.

241 more potent activator of transcription than ER beta in bone, uterine, and mammary cells.

242 induced higher levels of transcription than ER beta in the presence of 17 beta-estradiol.

243 d the major groove of the DNA helix but that ER beta failed to induce a directed DNA bend.

244 C/ in situ hybridization study revealed that ER beta mRNA and immunoreactivity were colocalized in ne

245 Immunofluorescence microscopy showed that ER beta protein was present in glycoprotein (GP) IIb(+)

246 ell lines, in which it was demonstrated that ER-beta mRNA was significantly up-regulated in the resis

247 Our data indicate that ER-beta is expressed at higher levels in Caco-2 cells an

248 The findings indicated that ER-beta-bearing inhibitory neurons project onto other GA

249 By Western blot analysis, we show that ER-beta protein is expressed in all cancer cell lines te

250 Evidence is also provided to show that ER-beta-catenin down-regulates cadherin protein levels.

251 We have recently shown that ER-beta mRNA is regulated by estradiol (E2) and that ant

252 after osmotic manipulation, suggesting that ER-beta expression was not driven by ligand availability

253 mediated ischemic protection suggesting that ER-beta plays a key role in mediating the beneficial eff

254 d the major groove of the DNA helix, but the ER beta DBD and hinge region failed to bend ERE-containi

255 The ER-beta agonist DPN did not mimic the effect of estradio

256 The ER-beta isoform is functionally similar to ER-alpha but

257 The ER-beta variant protein is predicted to lack part of the

258 ligand 16alpha-iodo-17beta-estradiol and the ER-beta selective ligand genistein failed to elicit ERK

259 Sequence analysis of the ER-beta variant PCR product revealed the absence of 139

260 reated ovariectomized C57BL/6J mice with the ER-beta selective agonist 2,2-bis(4-hydroxyphenyl)-propr

261 odies were made against a peptide within the ER-beta B domain.

262 This ER-beta deletion corresponds precisely to the entire exo

263 act on the OT system at two levels: through ER-beta, they regulate the production of OT in the hypot

264 Thus, ER beta may be a useful prognostic factor in patients wi

265 Thus, ER-beta behaves like a noncanonical type-I receptor, and

266 Thus, ER-beta exhibits extensive colocalization with a subclas

267 terestingly hPMC2 interacts more strongly to ER beta when compared with ER alpha.

268 enous gene, this element is nonresponsive to ER-beta but confers estrogen-dependent inhibition of tra

269 tence of additional relationships related to ER-beta and enzymes involved in hormone metabolism.

270 lly prepared aglycons bound significantly to ER-beta, except for 27-deoxyactein aglycon, which showed

271 P was shown to recognize in vitro translated ER beta, but not ER alpha, as well as a 60-kDa protein f

272 To test the specificity of DPN, we treated ER-beta-knockout mice with DPN.

273 f ER-beta that is coexpressed with wild-type ER-beta in the ER-alpha-negative, estrogen-independent b

274 nd E2 with lower affinity than the wild-type ER-beta protein.

275 estrogen action was signaled exclusively via ER-beta in normal human PrECs.

276 s hormone, no studies have addressed whether ER beta can be similarly regulated.

277 R alpha, either alone or in combination with ER beta.

278 ressed more abundantly on EPCs compared with ER beta.

279 discernable activity with ER-alpha, but with ER-beta, E(2) was displaced with an IC(50) of 125 microM

280 or ER-alpha mRNA was very weak compared with ER-beta mRNA.

281 ol and testosterone were not correlated with ER-beta mRNA expression after osmotic manipulation, sugg

282 es and subjected to binding experiments with ER-beta.