ライフサイエンスコーパス: ESEEM

1 ESEEM also demonstrates ligation of two water molecules

2 ESEEM analysis revealed that two histidines are bound eq

3 ESEEM measurements support a differential ability of Mn(

4 ESEEM of the 2H-labeled substrate radical states in wild

5 ESEEM spectra demonstrate that the histidine imidazole c

6 ESEEM spectra have been used to determine the orientatio

7 ESEEM spectra of altered MoFe proteins, which were produ

8 ESEEM spectra of the previously characterized C24A and C

9 14N ESEEM from a hyperfine-coupled protein nitrogen in wild

10 However, an 15N ESEEM peak was observed in parallel chloride-depleted PS

11 ientation-selective Ka- and Q-band ENDOR, 1D ESEEM, and HYSCORE spectra of (14)N and (15)N-labeled mi

12 The 2D ESEEM (HYSCORE) experiments with the Q(H) site SQ in the

13 isotope effect for Y(Z) oxidation and by 2H ESEEM measurement of hydrogen-bond exchange.

14 retains four water ligands as measured by 2H ESEEM spectroscopy.

15 sition (adjacent to the sulfonate group), 2H ESEEM spectra show features that arise from dipole-dipol

16 With 2H ESEEM modulation comparable to that of Y(D)*, Y(Z)* woul

17 2H2O ESEEM spectra showed that the [2Fe-2S] cluster is access

18 terated at both C1 and C2 show an additional ESEEM feature at the deuterium Larmor frequency.

19 ligates the Mn cluster, we have conducted an ESEEM study of D1-H332E PSII particles.

20 (14)N ENDOR and ESEEM data are most consistent with one of these dicoppe

21 Analysis of pulsed ENDOR and ESEEM experiments investigating the proximity of deutero

22 In X-band ENDOR and ESEEM spectra, a weakly coupled nitrogen is visible, mos

23 ENDOR and ESEEM spectroscopy of Cu(II)-PcoC and the (15)N- and Met

24 MO have been investigated by EPR, ENDOR, and ESEEM spectroscopies in combination with metal content a

25 ess this issue, we have conducted an EPR and ESEEM (electron spin-echo envelope modulation) study of

26 Thus, the EPR and ESEEM data indicate the presence of a Mn2+ binding site

27 The EPR and ESEEM measurements evince a protein-mediated force on th

28 The interpretation of the EPR and ESEEM spectra was facilitated by a theoretical analysis

29 d forms of the enzyme, and Mn(II) CW-EPR and ESEEM spectra were recorded.

30 s been definitively characterized by EPR and ESEEM spectroscopy.

31 a combination of X-band EPR, S-band EPR, and ESEEM, along with a library of modified peptides designe

32 Ka band ESEEM spectroscopy was used to determine the hyperfine (

33 Companion S-band EPR, X-band ESEEM, and HYSCORE experiments performed on a library of

34 sed the possibility of a correlation between ESEEM spectroscopic properties and the nitrogenase pheno

35 oxin was further characterized by EPR and by ESEEM spectroscopy and was found to differ only marginal

36 ies, designated H, has been characterized by ESEEM spectroscopy using a combination of (14,15)N isoto

37 e too large or anisotropic to be detected by ESEEM analyses conducted at 9.2 GHz.

38 equatorial water on copper, as determined by ESEEM analysis.

39 2+ in this ternary complex was determined by ESEEM studies in D2O to be two fewer than on the average

40 nitrogens, one near the "exact cancellation" ESEEM condition and the other more weakly coupled.

41 In the present study, we combine ESEEM spectroscopy with site-directed spin labeling (SDS

42 rs of the extracted membrane lipids, the D2O-ESEEM intensities of fully charged n-SASL decrease progr

43 alpha-359-arginine is the source of the deep ESEEM N1 modulation; (ii) one or both of the amide nitro

44 model system, we demonstrate that deuterium ESEEM can detect water permeation along the lipid-expose

45 The two-dimensional ESEEM (HYSCORE) data have directly identified N(epsilon)

46 In the two-pulse frequency domain ESEEM spectrum of the 9.2 GHz S(2) state multiline EPR s

47 Simulation of the time- and frequency-domain ESEEM requires two types of coupled (2)H.

48 coupling constants with results from earlier ESEEM and electron nuclear double resonance (ENDOR) stud

49 Stimulated echo ESEEM data were divided to eliminate interference from 1

50 se variants and investigated by Q-band ENDOR/ESEEM are identical to states, denoted H and I, formed o

51 We report CW-EPR, ESEEM, and structural NMR results, as well as DFT calcul

52 The results presented here establish ESEEM as a highly informative technique for SDSL studies

53 N nuclear quadrupole parameters derived from ESEEM simulations and those computed by using density fu

54 The best (2)H ESEEM time- and frequency-domain simulations are achieve

55 One peptide nitrogen has ESEEM peaks near 0.7, 2.0, 2.85, and 5.0 MHz with isotro

56 -band EPR and (14/15)N, (1,2)H ENDOR/HYSCORE/ESEEM measurements that characterize the N-atom(s) and p

57 l nitrogen modulation observed near 5 MHz in ESEEM spectra of the S(2) state multiline EPR signal of

58 N1 interaction with FeMo-cofactor; and (iv) ESEEM can be used to detect slight reorientations of FeM

59 n the present work, Q-band CW EPR and (95)Mo ESEEM spectroscopy reveal such samples also contain a co

60 from electron spin echo envelope modulation (ESEEM) allowed to model the N-terminal protein section,

61 and electron spin echo envelope modulation (ESEEM) analysis of Mn(2+)-substituted cytochrome c oxida

62 ulse electron spin-echo envelope modulation (ESEEM) and 4-pulse 2-dimensional hyperfine sublevel corr

63 ulse electron spin echo envelope modulation (ESEEM) and four-pulse two-dimensional hyperfine sublevel

64 h as electron spin echo envelope modulation (ESEEM) and hyperfine sublevel correlation (HYSCORE) spec

65 sing electron spin echo envelope modulation (ESEEM) and hyperfine sublevel correlation spectroscopy (

66 14N electron spin-echo envelope modulation (ESEEM) arising from a nitrogen nucleus (N1) coupled to t

67 and electron spin echo envelope modulation (ESEEM) experiments have been performed.

68 the electron spin echo envelope modulation (ESEEM) from (2)H-hyperfine interaction with D2O is deter

69 ated electron spin echo envelope modulation (ESEEM) have been studied in either thylakoid membranes,

70 n of electron spin-echo envelope modulation (ESEEM) spectra from multi-crystalline Cu(2+)-insulin wit

71 band electron spin-echo envelope modulation (ESEEM) spectra of lpH SO were also analyzed.

72 Electron spin-echo envelope modulation (ESEEM) spectra of these Cu-RC proteins have been obtaine

73 Electron spin echo envelope modulation (ESEEM) spectral results indicate multiple weakly hyperfi

74 and electron spin-echo envelope modulation (ESEEM) spectroscopic methods.

75 and electron spin-echo envelope modulation (ESEEM) spectroscopic properties of ethanolamine ammonia-

76 and electron spin-echo envelope modulation (ESEEM) spectroscopies, can help us understand how metal

77 ulse electron spin-echo envelope modulation (ESEEM) spectroscopy in the disordered solid state.

78 ulse electron spin-echo envelope modulation (ESEEM) spectroscopy in the disordered solid state.

79 ver, electron spin-echo envelope modulation (ESEEM) spectroscopy indicates that all nine lectins are

80 Electron spin-echo envelope modulation (ESEEM) spectroscopy is a well-established technique for

81 Electron spin echo envelope modulation (ESEEM) spectroscopy, a pulsed electron spin resonance te

82 ctable by electron spin envelope modulation (ESEEM) spectroscopy, is observed in WT enzyme but not in

83 band electron spin-echo envelope modulation (ESEEM) spectroscopy.

84 sing electron spin echo envelope modulation (ESEEM) spectroscopy.

85 ulse electron spin-echo envelope modulation (ESEEM) spectroscopy.

86 d by electron spin-echo envelope modulation (ESEEM) spectroscopy.

87 ency electron spin echo envelope modulation (ESEEM) studies of this species prepared with (33)S-label

88 and electron spin-echo envelope modulation (ESEEM) studies on the Cu(II) derivative provided evidenc

89 y an electron spin echo envelope modulation (ESEEM) study conducted with [(15)N]histidine-labeled pho

90 ulse electron spin echo envelope modulation (ESEEM) technique was used to detect weakly coupled (2)H-

91 Electron spin-echo envelope modulation (ESEEM) was used to search for spin transitions of 15N nu

92 PR), electron spin-echo envelope modulation (ESEEM), and UV-vis spectroscopies have greatly expanded

93 ave, electron spin-echo envelope modulation (ESEEM), pulsed electron-nuclear double resonance (ENDOR)

94 ar double resonance/ESE envelope modulation (ESEEM)] reveal differential details of the in vivo Mn(2+

95 uces dominating contribution to X-Band (14)N ESEEM spectra.

96 The N2 ESEEM pattern is more obvious when unmasked by substitut

97 wo independent approaches: (a) comparison of ESEEM from cob(II)alamin with ESEEM from cob(II)inamide-

98 Theoretical simulations of ESEEM spectra guided by structure modeling suggest that

99 The "out-of-phase" (OOP) ESEEM attributed to the [P700+ A1-] radical pair has bee

100 electron spin echo envelope modulation (OOP-ESEEM).

101 This study shows that OOP-ESEEM is well-suited for probing the detailed structural

102 extracted by time-domain fitting of the OOP-ESEEM.

103 We further demonstrate that (31)P ESEEM is able to identify channel residues that interact

104 Present ESEEM data reveals that two kinds of 14N modulations are

105 A previous ESEEM study on altered MoFe proteins generated by substi

106 tion, a compilation of results from previous ESEEM studies of copper proteins reveals that the asymme

107 hrough the use of one-dimensional four-pulse ESEEM and subsequent analysis of the sum combination pea

108 Three-pulse ESEEM experiments were performed by using microwave puls

109 Three-pulse ESEEM of the natural abundance Sr sample exhibits no det

110 We performed three-pulse ESEEM on this modified multiline signal of the Mn cluste

111 By comparing the ratioed three-pulse ESEEM spectra of a control, untreated PSII sample in 50%

112 Using three-pulse ESEEM spectroscopy, we have successfully detected the tw

113 The three-pulse ESEEM spectrum of the wild-type CF(1)-ATPase with VO(2+)

114 Simulation of three-pulse ESEEM with a numerical matrix diagonalization procedure

115 Simulation of the (2)H/(1)H quotient ESEEM (obtained at two microwave frequencies, 8.9 and 10

116 Recent ESEEM studies of p21 in solution, however, place threoni

117 We now report ESEEM studies on altered MoFe proteins with substitution

118 spin-echo envelope modulation spectroscopy (ESEEM) to characterize the protein-cofactor interactions

119 spin echo envelope modulation spectroscopy (ESEEM).

120 or the electron spin, analysis of the (87)Sr ESEEM modulation depth via an analytic expression sugges

121 The ESEEM spectra of Species 1 prepared in (17)O-enriched wa

122 The ESEEM spectra of the mutants do not show evidence of an

123 The ESEEM spectra of wild-type ferredoxin displayed weak 14N

124 llumination treatment induces a shift in the ESEEM frequency.

125 Changes in the ESEEM spectra for different samples correlate directly t

126 s of (14)N features are distinguished in the ESEEM spectra.

127 +/- 0.2 MHz) of the phosphorus signal in the ESEEM spectrum established the formation of an inner sph

128 nuclear quadrupole induced splitting in the ESEEM spectrum of (87)Sr enriched PSII samples is relate

129 etism of Y(.)(z), we are able to observe the ESEEM spectrum of deuterated acetate interacting with on

130 A good fit of the ESEEM data indicates two (2)H dipolar hyperfine coupling

131 procedure for high-precision analysis of the ESEEM data of the MoFe proteins shows that the deep wild

132 Numerical simulations of the ESEEM data suggest that the coupling is primarily isotro

133 Correlations of the ESEEM properties and catalytic activities of the altered

134 ii) explore the mechanistic relevance of the ESEEM signatures to nitrogenase activity; and (iii) stud

135 Furthermore, a comparison of the ESEEM spectra obtained in H(2)O and D(2)O demonstrates t

136 2qQ approximately 3.3 MHz) indicate that the ESEEM effect is most likely due to 14N from the polypept

137 We conclude that the ESEEM spectrum of QA- is the result of interactions of t

138 These ESEEM spectra resemble those observed for Cu(2+) RCs fro

139 MoFe proteins shows that the deep wild-type ESEEM modulation (denoted N1) has a hyperfine-coupling c

140 cetate-treated PSII and untreated PSII using ESEEM.

141 e hydration level of Mn2+ in this site using ESEEM (electron spin-echo envelope modulation) spectrosc

142 peptide nitrogen is that of D2-Ala260, with ESEEM peaks near 0.6 and 1.5 MHz and an isotropic hyperf

143 comparison of ESEEM from cob(II)alamin with ESEEM from cob(II)inamide-ligand model compounds and (b)