ライフサイエンスコーパス: EST

1 EST data generated from 14 apple genotypes were download

2 EST libraries were used to generate unigene clusters and

3 EST sequence assembly and analysis provide unique benefi

4 EST sequences were generated from a normalized cDNA pool

5 imals and plants, has generated over 500,000 ESTs and 1.2 million genome survey sequences from more t

6 We isolated over 8,000 ESTs from the glandular trichomes of L. x intermedia flo

7 SNP mining from 183,118 ESTs sequenced from 17 cDNA libraries yielded approximat

8 e PCR was used to validate modulation of 127 ESTs that were differentially expressed in developing an

9 (ESTs) were upregulated in juveniles, and 28 ESTs were upregulated in adults.

10 +) cells, including the de novo-generated 3' ESTs and the existing sequences of full-length cDNAs, ES

11 Of the 335 ESTs identified, 211 share significant homology with kno

12 espite being optimized for SNP mining in 454 EST data, it is flexible enough to automate the analysis

13 the mapping of 64,000 Fagus grandifolia 454 ESTs and unigenes to the poplar genome.

14 A total of 27,551 ESTs was obtained from five cDNA libraries constructed f

15 Sequence analysis of the 1,679 ESTs resulted in the identification of 835 distinct tran

16 ithography arrays, composed of probes of 832 EST-unigenes from a subtracted, fruit development, cDNA

17 ce nsLtp genes and wheat (Triticum aestivum) EST sequences indexed in the UniGene database.

18 All ESTs are in the Gateway vector to facilitate functional

19 Additionally all ESTs from the representative contigs when checked agains

20 rithm to Mus and Rattus, and 34 - 54% of all ESTs could be assigned to a biological process gene onto

21 Indeed, > 20% of all ESTs represent genes encoding putative cellulases, inclu

22 Approximately 82% of all ESTs were identified using the BLASTX algorithm to Mus a

23 Approximately 40% of all ESTs were specific to only one location in the Mus genom

24 cripts predominately encoded PMF (45% of all ESTs) and PRF (15%), with less than 0.5% SPF.

25 arkers (538 Bin, 258 AFLPs, 175 SSRs, and an EST).

26 AaSDR-1 was isolated as an EST in a library of the corpora allata-corpora cardiaca

27 work in its ability to accurately assign an EST to a gene, statistically measure differential expres

28 dataset for sequence mining, we generated an EST database by 454 sequencing of the caribfly, Anastrep

29 hor the translation start codon, searched an EST database with the 3' end of the genomic DNA sequence

30 ng the most highly enriched (31% align to an EST contig), while the HMPR clones exhibited exceptional

31 For each splicing version of an exon, an ESTs count is given, specifying the frequency of the eve

32 ergo hybridization approach and all anchored ESTs were functionally annotated via blast analysis.

33 ence information, phylogenetic analysis, and EST data with published reports on tobacco TF function.

34 analysing novel transcriptome assemblies and EST sequences in the absence of a reference genome.

35 nd economical than traditional PCR-based and EST-based methods and provides a scalable strategy for g

36 transcriptomic data such as RACE, CAGE, and EST into its model to further increase isoform discovery

37 of EST by sepsis is NF-kappaB dependent and EST is a NF-kappaB-target gene.

38 2 and 3, through searches of genomic DNA and EST databases.

39 The DNA dosage and EST expression of CNV12, which overlap with an obesity r

40 he reciprocal regulation of inflammation and EST may represent a yet-to-be-explored mechanism of endo

41 4a and vsx1) and pectoral fin bud (klf2b and EST AI722369) as candidate targets for Sox9.

42 lsDB contains databases for DArT markers and EST sequences, and links to a draft genome sequence for

43 onal data such as transcript (microarray and EST evidence) and protein expression data.

44 arative genomics, gene predictions, mRNA and EST alignments and physiological tissue expression.

45 mic sequences, annotated coding regions, and EST data.

46 e types of DNA repeats, structured RNAs, and EST-supported loci with convergent or paralogous transcr

47 gene models using evidence from RNA-Seq and EST alignments.

48 c data from both whole genome sequencing and EST projects provide opportunities for identifying many

49 compared with the current transcriptome and EST databases.

50 A survey of genomes and ESTs revealed AAH-like sequences in gymnosperms, mosses,

51 -AG 3' junctions absent from gene models and ESTs (11% increase to the current annotation).

52 , evolutionary conservation of DNA, mRNA and ESTs of the target species, and retroposed genes.

53 analytical tools that identify and annotate ESTs originating from specific mRNA populations.

54 f cDNA libraries and generated and annotated ESTs from two species of corals, Acropora palmata and Mo

55 However for large datasets such as ESTs, manual extraction of the information from the batc

56 sets supported with evidence tracks such as ESTs/cDNAs, small RNA sequences and GC composition domai

57 Annotation System), which performs automated EST cleaning, clustering, assembly and annotation on a d

58 P gene, MusaSAP1, was identified from banana EST database and was subsequently characterized by overe

59 of post-ERCP pancreatitis was found between EST and EPBD groups in any one group of patients with th

60 difference in post-ERCP pancreatitis between EST and EPBD.

61 Both EST data and experimental data suggest that this variant

62 age, physical and integrated maps, a catfish EST contig viewer with SNP information overlay, and GBro

63 ping of polyA signals, and supported by cDNA/EST and ditags data.

64 We compiled a large cDNA/EST dataset for vertebrates (75 genes for 129 taxa) to a

65 the existing sequences of full-length cDNAs, ESTs, and serial analysis of gene expression (SAGE) tags

66 e tags (ESTs) in GenBank dbEST and community EST assemblies were either over- or undertrimmed in term

67 By comparing EST abundance, many genes showing apparent differential

68 TOBFAC also contains EST data, a list of published tobacco TFs and a list of

69 lies hybridizing to randomly selected cotton ESTs.

70 evaluate nematode genomic sequences (curated EST contigs) against the annotated Kyoto Encyclopedia of

71 n biosynthesis, we previously performed deep EST profiling of fenugreek (Trigonella foenum-graecum L.

72 essed and non-stressed banana tissue derived EST data sets and later overexpression in transgenic ban

73 y of both Sanger- and pyrosequencing-derived ESTs resulted in 34,495 unique sequences with 1,110 sequ

74 lating fruiting body development and develop EST-SSR markers assessing the genetic value of breeding

75 We have developed "EST Express", a suite of analytical tools that identify

76 otomy/endoscopic papillary balloon dilation (EST/EPBD) with negative ERC finding.

77 existing, largely unannotated dinoflagellate EST datasets (DinoEST).

78 transcript annotations and 666,153 dipteran EST sequences we have identified 44 putative conserved p

79 s, QTL and alignments to complementary DNAs, ESTs and protein homologs.

80 ich were encoded by the up- or downregulated ESTs without homologues (NH) suggested that seven of the

81 that exert deleterious impacts on downstream EST-based applications.

82 itional SNPs were identified from the entire EST collection available for the species.

83 encoding cytochrome P450s (CYP), esterases (EST), or glutathione transferases (GST) and at 12 previo

84 ione-S-transferase (GST), general esterases (ESTs) and phenol oxidase (PO) decreased in the insect du

85 based on bioinformatics analysis of existing EST libraries, indicating a potential role of BxPrx in b

86 d primers for these differentially expressed ESTs in order to validate the results using Q-PCR.

87 of the chorion locus, we performed extensive EST analysis, constructed a bacterial artificial chromos

88 1-EU977132 (FLI cDNA) and FK944382-FL482108 (EST).

89 The patterns of missing data typical for EST-derived alignments greatly compromise the accuracy o

90 ning clones from ORESTES (Open Reading frame ESTs) libraries, suppression subtractive hybridization (

91 studies, we have constructed a user-friendly EST annotation pipeline with comparison tools on an inte

92 alogs could be identified with accuracy from EST data alone when compared to the actual gene sequence

93 EGG annotations for data sets resulting from EST sequencing projects both rapidly and efficiently.

94 16,952 in silico-verified poly(A) sites from EST sequencing traces based on Chlamydomonas Genome Asse

95 method for inferring phylogenetic trees from EST-based incomplete MSA data.

96 ork involved screening 492 loci derived from ESTs on a large panel of wild, primitive (i.e., landrace

97 Gene family identification from ESTs can be a valuable resource for analysis of genome e

98 re response of more than 400 annotated genes/ESTs among these populations, reflected by a shared, but

99 However, annotations in the C. gigas EST library suggested the presence of putative Jumonji g

100 om the A and B genomes, while cv Recital has ESTs from the A and D genomes.

101 omputed by diverse gene finders, BLAST hits, EST matches, and splice site predictions; learned parame

102 f this study is to determine whether and how EST plays a role in human adipogenesis.

103 However, ESTs associated with non-plastid enzymes of oil biosynth

104 nstrate that an integrated analysis of human ESTs provides a robust platform to identify the immune t

105 over 100 000 isoforms have been detected in EST libraries, and at least 75% of human genes have at l

106 spect to neighborhood factors as outlined in EST.

107 y upregulated genes were also represented in EST libraries derived from diverse diatoms grown under v

108 auses unclean spurious sequences retained in ESTs that exert deleterious impacts on downstream EST-ba

109 of the transcriptome of Zea mays, including ESTs representing 484,032 cDNA clones from 53 libraries

110 Using GMAP, 156,963 individual ESTs were mapped to the genome successfully, with their

111 hen developed 26 polymorphic and informative EST-SSR markers to assess the genetic diversity in 82 st

112 unogene database, representing an integrated EST road map of gene expression in human immune cells, w

113 eline with comparison tools on an integrated EST service website, Bio301.

114 splay is continually updated with the latest ESTs and BAC sequences.

115 data as well as a collection of full length ESTs from several organs, developmental stages and stres

116 The catalog contains 93% of a Lingulodinium EST dataset deposited in GenBank and 94% of the enzymes

117 tabase, while 9.2% matched to the bin mapped ESTs.

118 Mechanistically, EST ablation attenuates sepsis-induced inflammatory resp

119 Mechanistically, EST promoted adipogenesis by deactivating estrogens.

120 pyrosequencing, we generated over 7 million ESTs from four stages of developing seeds of Ricinus com

121 s from NCBI (RefSeq), predicted gene models, ESTs and whole-genome tiling array data representing sev

122 y high when the contigs contain four or more EST sequences with the minor allele sequence being repre

123 particular, contigs containing four or more ESTs should be used and the minor allele sequence should

124 ection of rooted gene trees (for STAR and MP-EST methods) or unrooted gene trees (for NJst).

125 sis and several 'summary methods': BUCKy, MP-EST, minimize deep coalescence, matrix representation wi

126 species using three species tree methods-MP-EST, STAR and NJst.

127 tistical binning improves the accuracy of MP-EST, a popular coalescent-based method, and we use it to

128 and has outstanding accuracy-improving on MP-EST and the population tree from BUCKy, two statisticall

129 on-exon junctions observed in the human mRNA/EST transcripts, representing a significant increase in

130 library comparison functions using multiple EST libraries based on GO annotations for mining meaning

131 single Brachypodium BACs matched to multiple ESTs that were mapped to the same deletion bins, suggest

132 Using the new EST evidence, automated and manual curation has resulted

133 both Chinese Spring and Ae. tauschii, but no ESTs match this sequence and limited genomic sequences i

134 cer Institute, Clinical Trials (PDQ), number EST-9486.

135 This study identified numerous ESTs with putative involvement in the watermelon fruit d

136 3' end of the genomic DNA sequence to obtain ESTs from the farthest 3' end of the gene, and isolated

137 The proadipogenic activity of EST in humans was opposite to the antiadipogenic effect

138 Analyses of EST data show that alternative splicing is much more wid

139 Careful analyses of EST databases indicated that a homologous transcript als

140 g comparative in silico sequence analysis of EST clones, suggesting that global aberrant alternative

141 We demonstrate here by using analysis of EST databases, RT-PCR, in situ hybridization, and Northe

142 Analysis of EST levels from these oilseeds revealed both conserved a

143 ot8r, a platform for the rapid annotation of EST datasets with GO-terms, EC-numbers and KEGG-pathways

144 questions relevant to quality assessment of EST-derived SNPs.

145 A collection of EST sequences from a cDNA library made from young E. coc

146 The proadipogenic effect of EST can be recapitulated by using an estrogen receptor (

147 sitive correlation between the expression of EST and body mass index (BMI), as well as a negative cor

148 de models of sepsis induce the expression of EST and compromise the activity of oestrogen, an anti-in

149 e subjects, we showed that the expression of EST was low in preadipocytes but increased upon differen

150 The induction of EST by sepsis is NF-kappaB dependent and EST is a NF-kap

151 Inhibition of EST, at least in females, may represent a novel approach

152 Overexpression and knockdown of EST in ASCs promoted and inhibited differentiation, resp

153 In contrast, transgenic overexpression of EST promotes oestrogen deactivation and sensitizes mice

154 limited applicability of basic predictors of EST.

155 sequencing is greatly outpacing the rate of EST- and cDNA-sequencing projects.

156 of large number of SNPs, but reliability of EST-derived SNPs are relatively low because of sequencin

157 Our results revealed an essential role of EST in energy metabolism and the pathogenesis of type 2

158 8r is integrated with the PartiGene suite of EST analysis tools.

159 range: 0.04-2,597 g) to test the utility of EST and MTE in predicting excretion rates of nitrogen (E

160 Thus, we demonstrate the utility of EST profiling as a basis for further deconstruction of p

161 und to be most significant for validation of EST-derived SNPs: the contig size (number of sequences i

162 Analysis of ESTs and cDNAs shows that the genes are expressed in at

163 In this study we report that analysis of ESTs from the digestive system of Limnoria quadripunctat

164 This collection of ESTs will serve as the foundation for the future identif

165 of the Triticeae tribe since examination of ESTs from three barley cultivars also confirms a single

166 Here, we report the identification of ESTs whose expression in antisense orientation limited h

167 The larger number of ESTs in these 16 datasets provided reliable estimates of

168 A large resource of ESTs is to become available in catfish allowing identifi

169 ment measures, along with large resources of ESTs, should provide effective means for SNP identificat

170 In all four oilseeds, ESTs for Rubisco were present, suggesting its possible r

171 Sequence analysis on EST database of the model legume Medicago truncatula ena

172 We also find that, using only EST and cross-species evidence, the Gramene pipeline can

173 ene family members in Arabidopsis using only EST data and we describe how this strategy can be used t

174 Using only ESTs we were able to correctly predict at least one spli

175 cly available data, where microarray data or EST data are used to detect male-biased genes in D. mela

176 ity of estrogen sulfotransferase (SULT1E1 or EST), an enzyme important for the metabolic deactivation

177 ften with accompanying sequences of cDNAs or ESTs.

178 models (assembled expressed sequence tags or ESTs and mapped to a genome assembly) for P. dactylifera

179 Our EST-Genome-Browser can display annotated gene structures

180 nces between query cDNAs and the genome, our EST-Genome-Browser allows biologists to discover potenti

181 ic' variation (no more than two variants per EST-SSR genotype) and recently published chromosomal evi

182 examined the distribution of the Peromyscus ESTs across the rat genome finding markers on all rat ch

183 A previous EST study identified a MADS box transcription factor cod

184 pecific homopolymer sequences in error-prone EST/cDNA data or RNA-Seq data at a speed appropriate for

185 tested for the presence of dsx within public EST and genome sequencing projects representative of all

186 ated 14,588 (Ap) and 3,854 (Mf) high quality ESTs from five life history/symbiosis stages (spawned eg

187 It contained 7,862 high-quality ESTs (comprising 4,468 unigenes) obtained from mesocarp

188 Over 11.5 million high-quality ESTs were generated with 454 sequencing technology, and

189 We examined 309,278 raw EST sequencing trace files of C. reinhardtii and found t

190 s of CBDS but negative ERC, who had received EST/EPBD treatments was enrolled.

191 ded from NCBI and mapped against a reference EST assembly to identify Single Nucleotide Polymorphisms

192 Bio301 includes regular EST preprocessing, BLAST similarity search, gene ontolog

193 pressed-sequence tag simple-sequence repeat (EST-SSR)) primers to survey genetic variation in populat

194 one from testis, characterize the resulting ESTs, and describe their utility for mapping the Peromys

195 Published Sanger-ESTs (544 225) from Alamo, Kanlow, and 15 other cultivar

196 terns (NSP) between family members to screen EST-generated contigs.

197 It houses the complete genome sequence, ESTs and the entire body of literature relevant to Dicty

198 ctyBase houses the complete genome sequence, ESTs, and the entire body of literature relevant to Dict

199 and assemble 148 Mbp of expressed sequences (EST).

200 e transcriptome and the existence of several EST tags.

201 were monitored for expression via in silico EST analysis.

202 activity of each human GLTP gene, in silico EST evaluations, RT-PCR amplifications of GLTP transcrip

203 al hairpin-forming precursors within soybean EST and shotgun genomic sequences.

204 cilitate the annotation of non-model species EST datasets with well-defined ontologies that enable me

205 make it ideally suited for non-model species EST-sequencing projects.

206 Oryza sativa, we show that the cross-species ESTs from within monocot or dicot class are a valuable s

207 into other evidences-GenBank mRNAs, spliced ESTs, CAGE promoter tags and mRNA-seq reads.

208 Ecological stoichiometry (EST) and metabolic theory of ecology (MTE) are theoretic

209 The role of one such EST, that of ectonucleoside triphosphate diphosphohydrol

210 eat cultivars for which there are sufficient ESTs show different patterns of expression, i.e., with c

211 Furthermore, the Sugarcane EST Database was extensively surveyed to identify lignin

212 ion, catalyzed by estrogen sulfotransferase (EST), is investigated here in detail.

213 Estrogen sulfotransferase (EST), the enzyme responsible for the sulfonation and ina

214 Estrogen sulfotransferase (EST/SULT1E1) is known to catalyze the sulfoconjugation a

215 ial role for the oestrogen sulfotransferase (EST or SULT1E1), a conjugating enzyme that sulfonates an

216 Surprisingly, EST ablation sensitizes mice to sepsis-induced death.

217 erexpression in both expressed sequence tag (EST) and serial analysis of gene expression (SAGE) datab

218 Expressed sequence tag (EST) data predicts multiple splice variants of both huma

219 e screened a Dionaea expressed sequence tag (EST) database and identified DmKT1 and DmHAK5 as candida

220 le Ixodes genome and expressed sequence tag (EST) database.

221 erized as part of an expressed sequence tag (EST) discovery project.

222 large collection of expressed sequence tag (EST) DNAs corresponding to approximately 20,000 human ge

223 DNA-AFLP to identify Expressed Sequence Tag (EST) fragments that are differentially expressed in resp

224 erated and sequenced expressed sequence tag (EST) libraries from thirteen diverse switchgrass cultiva

225 The expressed sequence tag (EST) methodology is an attractive option for the generat

226 btained ~2.6 million expressed sequence tag (EST) reads from Populus deltoides leaf transcriptome and

227 Our expressed sequence tag (EST) screen reveals that at least seven different AFP va

228 d and 9,816 cleansed expressed sequence tag (EST) sequences were obtained from random sequencing of 1

229 alysis based on deep expressed sequence tag (EST) sequencing allows quantitative comparisons of gene

230 cripts compared with expressed sequence tag (EST), which is instrumental in gene discovery and gene s

231 (Fagaceae) using 32 expressed sequence tag (EST)-derived microsatellite markers.

232 ated using RNA-seq, expressed sequence tags (EST), and reverse transcription PCR (RT-PCR).

233 egans, over 500,000 expressed sequence tags (ESTs) and nearly 600,000 genome survey sequences (GSSs)

234 A. flavus expressed sequence tags (ESTs) and whole-genome sequencing have been completed.

235 stitute to generate expressed sequence tags (ESTs) as an inexpensive and efficient solution for gene

236 ies of wheat using expression sequence tags (ESTs) as the reference in lieu of a complete genome sequ

237 ed 856 high-quality expressed sequence tags (ESTs) derived from unamplified primary cDNA libraries co

238 th information from expressed sequence tags (ESTs) for transcriptomic analysis.

239 ate a collection of expressed sequence tags (ESTs) from C. zeae-maydis and evaluate their expression

240 ement, we generated Expressed Sequence Tags (ESTs) from the transcriptome of Pythium ultimum DAOM BR1

241 mation derived from expressed sequence tags (ESTs) has been constructed for functional description of

242 domonas reinhardtii expressed sequence tags (ESTs) in GenBank dbEST and community EST assemblies were

243 r identification of expressed sequence tags (ESTs) in human cells indicated that cleavage activity of

244 ome-wide screen for expressed sequence tags (ESTs) that when transcribed in the antisense direction i

245 method of assigning Expressed Sequence Tags (ESTs) to genes and secondly, by using a novel likelihood

246 mately 40,000 human expressed sequence tags (ESTs) to randomly inactivate chromosomal genes in a bovi

247 Expressed sequence tags (ESTs) were generated from two tetraploid switchgrass gen

248 Some 221 expressed sequence tags (ESTs) were upregulated in juveniles, and 28 ESTs were up

249 nces, unigenes from expressed sequence tags (ESTs), markers, trait loci, genetic maps, genes, taxonom

250 a library of human expressed sequence tags (ESTs), we randomly inactivated host chromosomal genes in

251 For example, expressed sequence tags (ESTs), which are partial sequences of expressed genes, a

252 ll-length cDNAs, or expressed sequence tags (ESTs)-in the species of interest.

253 50,000 cap-trapped expressed sequence tags (ESTs).

254 otal of 1.5 million expressed sequence tags (ESTs).

255 Average lengths of placenta and testis ESTs were 711 bp and 826 bp, respectively.

256 We conclude that EST is a proadipogenic factor which may serve as a drugg

257 The EST data establish a foundation for future studies in ev

258 The EST levels for several genes potentially involved in acc

259 sesquiterpene synthases identified from the EST collection were expressed in Escherichia coli.

260 constructed full-length transcripts from the EST sequences.

261 e genes retrieved significant matches in the EST database, while 9.2% matched to the bin mapped ESTs.

262 evealed multiple transcripts not seen in the EST databases.

263 ultiple units (multimer) were present in the EST library.

264 Through analysis of the EST profiling data, we identified genes known to be invo

265 The ESTs assembled into a set of primarily stage-specific cl

266 The ESTs were assembled into 3,619 unigenes (1,101 contigs a

267 The ESTs, grouped into 4088 clusters and 531 singlets, repre

268 In 200 of the ESTs, we also noted editing within a specific 13 nucleot

269 Using the Ecological Systems Theory (EST) as a model, we sought to examine the strength of th

270 These EST-SSRs exhibited high transferability in closely relat

271 These ESTs allow for specific PCR amplification and broad cove

272 The expression profile of these ESTs in L. x intermedia and its parents L. angustifolia

273 For two of these ESTs we tested fourteen primer pairs each and using stan

274 Using this EST evidence, a combination of automated and manual rean

275 ovata Forsk) seed mucilaginous layer through EST profiling.

276 elopment and validation of a high-throughput EST-derived SNP assay for cowpea, its application in con

277 o of four GGPPS-like candidates from tobacco EST databases encode bona fide GGPPS that can interact w

278 Of the total ESTs associated with a function, an unusually large numb

279 ated and sequenced 1,679 random transcripts (ESTs) from cDNA libraries from the midguts of female tic

280 entify candidate genes in the basil trichome EST database.

281 Based on the P values (P<0.05), Fifty-two ESTs were found as differentially expressed genes and we

282 Independent of the species and tissue type, ESTs for core fatty acid synthesis enzymes maintained a

283 SNP every 187 bp from a total of 6888 unique EST contigs.

284 s, and demonstrate the utility of the unique EST-derived shRNA library for functional genetics studie

285 ntified three hundred and thirty-five unique ESTs that are differentially regulated by at least two-f

286 To tackle these issues, we used EST data from more than 200 species and provided the mos

287 BLAST analysis using EST sequences harboring SNPs with the highest associatio

288 -generation linkage map constructed by using EST-derived microsatellites and SNPs, laying a framework

289 catfish genome (N=29) was constructed using EST-based microsatellite and single nucleotide polymorph

290 perm expressed genes were investigated using ESTs produced from each sperm type; differential express

291 sequenced and analyzed over 60,000 verified EST reads from a variety of cellular growth and developm

292 AP13 and VS16 ESTs were assembled into 77 854 and 30 524 unique transc

293 genes were BLASTN compared against the wheat EST database and deletion bin mapped wheat ESTs.

294 Wheat ESTs that mapped to the pericentromeric region of the gr

295 t EST database and deletion bin mapped wheat ESTs.

296 Genomewide comparison of wheat ESTs that mapped to centromeric regions against rice gen

297 ium genome will be useful for ordering wheat ESTs within the deletion bins and developing specific ma

298 the Cen8 kinetochore region, and three wheat ESTs, BJ301191, BJ305475, and BJ280500, with similarity

299 t tissues-bone marrow, brain and kidney-with EST data to improve the annotation of the rat genome.

300 nt measures should be used when working with EST-derived SNPs.