ライフサイエンスコーパス: ETD

1 ETD fragmentation produced diagnostic fragment ions indi

2 ETD improved phosphopeptide identification, while PQD pr

3 ETD is a powerful MS/MS technique and does not compromis

4 ETD tandem mass spectrometry (MS/MS) provides extensive

5 ETD, however, has shown limited applicability to doubly

6 ETD-MS/MS of these labeled peptides also produces a peak

7 z (*) -type product ions resulting from 226 ETD product ion spectra can be assigned to a single, cor

8 formation of unique w-ions employing MS(3) (ETD-HCD) for rapid Ile/Leu distinction.

9 he ETD model was trained with more than 7000 ETD spectra, with and without supplemental activation.

10 es, compared to either 2,801 (CAD) or 5,874 (ETD) phosphopeptides.

11 rithm to score potential assignments against ETD-MS/MS data, we applied the method to glycopeptides g

12 AI-ETD achieves these gains through improved quality of fra

13 AI-ETD also outperformed HCD, generating more matching frag

14 AI-ETD increased the number of c- and z-type product ions f

15 AI-ETD provided the greatest sequence coverage for all five

16 AI-ETD, which leverages infrared photon bombardment concurr

17 introduce a new activation scheme called AI-ETD+ that combines AI-ETD in the high pressure cell of t

18 ation scheme called AI-ETD+ that combines AI-ETD in the high pressure cell of the QLT with a short in

19 vated ion electron transfer dissociation (AI-ETD) for top down protein characterization, showing that

20 vated ion electron transfer dissociation (AI-ETD) on a quadrupole-Orbitrap-linear ion trap hybrid MS

21 vated ion electron transfer dissociation (AI-ETD), a recently introduced method for enhanced ETD frag

22 (ETD) reactions, i.e., activated ion ETD (AI-ETD), significantly increases dissociation efficiency re

23 When combined with HCD, AI-ETD improved the protein sequence coverage by more than

24 can significantly improve localization in AI-ETD spectra.

25 extensive generation of fragment ions in AI-ETD+ substantially increases peptide sequence coverage w

26 to generating more unique fragment ions, AI-ETD provided greater protein sequence coverage compared

27 In all, the effectiveness of AI-ETD across the entirety of the m/z spectrum demonstrates

28 ns due to the infrared photoactivation of AI-ETD and show that modifying phosphoRS (a phosphosite loc

29 Here we present the performance of AI-ETD for identifying and localizing sites of phosphorylat

30 Finally, we demonstrate the utility of AI-ETD in localizing phosphosites in alpha-casein, an appro

31 e we describe the first implementation of AI-ETD on a quadrupole-Orbitrap-quadrupole linear ion trap

32 We then characterize the performance of AI-ETD using standard peptides in addition to a complex mix

33 First, we show that AI-ETD can be implemented in a straightforward manner by fa

34 Using 90 min analyses we show that AI-ETD can identify 24,503 localized phosphopeptide spectra

35 des using LC-MS/MS, showing not only that AI-ETD can nearly double the identifications achieved with

36 wn protein characterization, showing that AI-ETD definitively extends the m/z range over which ETD ca

37 this work highlights the analytical value AI-ETD can bring to both bottom-up and top-down phosphoprot

38 our analysis revealed that nearly 60% of all ETD-identified peptides carried two positive charges, wh

39 s can be effectively combined in alternating ETD and CID modes for a more comprehensive analysis.

40 a RP column and are gradient-eluted into an ETD-enabled mass spectrometer.

41 and that can subsequently be converted to an ETD reagent via gas-phase dissociation.

42 dicate that the search engines for analyzing ETD derived MS/MS spectra are still in their early days

43 mpare the method (ETcaD) to ion trap CAD and ETD.

44 fication of a peptide by consecutive CID and ETD fragmentation in an alternating mode.

45 verage over CID; and (iii) combining CID and ETD fragmentation increased the sequence coverage for an

46 eptides for a detailed comparison of CID and ETD fragmentation pattern.

47 we have systematically compared the CID and ETD fragmentation patterns for the large majority of the

48 nked peptide ions were identified by CID and ETD fragmentation, and the disulfide-dissociated (or par

49 characteristics were analyzed using CID and ETD tandem mass spectrometry.

50 d or electron transfer dissociation (CID and ETD).

51 by collision induced dissociation (CID) and ETD with linear quadrupole ion trap (LTQ)-Fourier transf

52 al CID (collision-induced dissociation)- and ETD (electron transfer dissociation)-MS/MS experiments.

53 of labile N-phosphorylated peptides, ECD and ETD are emerging as a complementary method.

54 results have for previously proposed ECD and ETD mechanisms are discussed.

55 n sequence coverage compared to both HCD and ETD.

56 cribed approach for online gas-phase HDX and ETD paves the way for making mass spectrometry technique

57 paration, column packing, sample loading and ETD analysis can be implemented in 5-15 h.

58 mass spectrometric analysis (HCD-MS(n)) and ETD (electron transfer dissociation)-HCD MS(3) analysis

59 Using the FLI tag and ETD, we identified Glu73 as the site of photoincorporati

60 Combining UVPD and ETD data afforded even deeper sequencing and greater ove

61 than either the Ascore (CAD) or the Ascore (ETD).

62 ful for characterizing glycopeptides because ETD generates information about both peptide sequence an

63 However, because ETD only yields comprehensive sequence coverage when the

64 teins can be proteolytically digested before ETD analysis, although digestion is not necessary for al

65 tion of fluoranthene are often used for both ETD and PTR reactions; the radical anion of fluoranthene

66 complementary information derived from both ETD and CID dissociation methods, peptide sequence and p

67 egrating the product ion information of both ETD and CAD data are evident by increased confidence in

68 on method was facilitated by performing both ETD and UVPD within the higher energy collisional dissoc

69 Protein analysis by ETD is thought to enhance the range of amino acid sequen

70 sphorylated peptides can be characterized by ETD.

71 induced dissociation (CID), fragmentation by ETD occurs randomly along the peptide backbone.

72 in ND(3) gas and subsequently fragmented by ETD.

73 the peptide backbone cannot be identified by ETD/HCD.

74 , the present work proposes (i) to reduce by ETD one of the two disulfide bridges of model peptides,

75 nating collision-induced dissociation (CID), ETD, and higher-energy collisional dissociation (HCD) sc

76 pes of spectra (including CID, HCD, ETD, CID/ETD and HCD/ETD spectra of trypsin, LysC or AspN digeste

77 spectra or even for spectral pairs (e.g. CID/ETD spectral pairs).

78 The novel HCD-pd-CID/ETD workflow combines the best possible decision tree de

79 This combined ETD and CID approach is particularly useful for characte

80 y high and the m/z ratio is low, we combined ETD with a targeted chemical derivatization strategy to

81 Combining ETD and CID, from this single study, we were able to ide

82 w ET efficiency, as compared to conventional ETD instrumentation, are the main drawbacks of this appr

83 f 10 mg or more vs placebo (dosage-dependent ETD range, -0.9 to -5.7 kg; P < .001).

84 ge-dependent estimated treatment difference [ETD] range for oral semaglutide vs placebo, -0.4% to -1.

85 rgine group; estimated treatment difference [ETD], -0.59% [95% CI, -0.74% to -0.45%]), meeting criter

86 electron transfer followed by dissociation (ETD) versus electron transfer without dissociation (ET,

87 C-MS/MS with electron-transfer dissociation (ETD) alone is used for the structural characterization o

88 Electron-transfer dissociation (ETD) analysis indicates the presence of K48 in these bra

89 mbination of electron-transfer dissociation (ETD) and collision induced dissociation (CID) fragmentat

90 onsisting of electron transfer dissociation (ETD) and collision induced dissociation (CID), in combin

91 les for both electron-transfer dissociation (ETD) and collision-induced dissociation (CID) experiment

92 g sequential electron transfer dissociation (ETD) and collision-induced dissociation (CID) steps, in

93 rediction of electron-transfer dissociation (ETD) and electron-capture dissociation (ECD) spectra of

94 ely obtained electron transfer dissociation (ETD) and higher-energy collisional dissociation (HCD) ta

95 or effecting electron-transfer dissociation (ETD) are described that involve either the storage of an

96 ry (MS) with electron transfer dissociation (ETD) capabilities.

97 od involving electron transfer dissociation (ETD) combined with ultraviolet photodissociation (UVPD)

98 Electron-transfer dissociation (ETD) constitutes a valuable tool to cleave the disulfide

99 Electron-transfer dissociation (ETD) delivers the unique attributes of electron capture

100 The use of electron transfer dissociation (ETD) facilitates this analysis because disulfide bonds a

101 ype protein, electron transfer dissociation (ETD) fragmentation has been used to pinpoint the residue

102 n (HCD), and electron transfer dissociation (ETD) fragmentation modes.

103 d species in electron-transfer dissociation (ETD) fragmentation.

104 Electron transfer dissociation (ETD) has improved the mass spectrometric analysis of pro

105 Electron transfer dissociation (ETD) has proven to be a promising new ion activation met

106 Electron-transfer dissociation (ETD) has recently been introduced as a fragmentation met

107 more common electron transfer dissociation (ETD) have been introduced and made widely available.

108 ion (ECD) or electron-transfer dissociation (ETD) have been successfully used for comprehensive phosp

109 outperformed electron transfer dissociation (ETD) in terms of sequence coverage, allowing the SETA re

110 n (HCD), and electron transfer dissociation (ETD) in terms of yielding the most comprehensive diagnos

111 on (ECD) and electron transfer dissociation (ETD) involve radical-driven fragmentation of charge-redu

112 orming rapid electron-transfer dissociation (ETD) ion/ion reactions on a hybrid linear ion trap-orbit

113 Electron transfer dissociation (ETD) is a recently introduced mass spectrometric techniq

114 Electron transfer dissociation (ETD) is a recently introduced mass spectrometric techniq

115 Electron transfer dissociation (ETD) is commonly used in fragmenting N-linked glycopepti

116 Electron transfer dissociation (ETD) is increasingly becoming popular for high-throughpu

117 Electron transfer dissociation (ETD) is the method of choice in analyzing these glycopep

118 pairs using electron transfer dissociation (ETD) markedly reduced adduct loss.

119 bitrap Velos electron-transfer dissociation (ETD) mass spectrometer (MS) was established to simultane

120 confirmed by electron transfer dissociation (ETD) mass spectrometry.

121 aphy (LC) to electron-transfer dissociation (ETD) mass spectrometry.

122 entional ECD/electron transfer dissociation (ETD) methods because it can be implemented using a stand

123 n (HCD), and electron transfer dissociation (ETD) MS/MS approach obtained 80% protein sequence covera

124 strated that electron transfer dissociation (ETD) of 4-plex iTRAQ labeled peptides cleaves at the N-C

125 on (ECD) and electron transfer dissociation (ETD) of doubly protonated electron affinity (EA)-tuned p

126 on (CAD) and electron transfer dissociation (ETD) on a single instrument.

127 tal-assisted electron transfer dissociation (ETD) or electron capture dissociation (ECD) provide vary

128 on (CID) and electron transfer dissociation (ETD) processes.

129 ions, CID of electron-transfer dissociation (ETD) products and CID of a metal-peptide complex formed

130 ation during electron transfer dissociation (ETD) reactions, i.e., activated ion ETD (AI-ETD), signif

131 ntations via electron-transfer dissociation (ETD) reactions.

132 nd 2981 from electron transfer dissociation (ETD) shows their great utility and complementarity for t

133 hods such as electron transfer dissociation (ETD) tandem mass spectrometry (MS/MS) as well as in char

134 Moreover, electron transfer dissociation (ETD) tandem mass spectrometry (MS/MS) on a large myoglob

135 ed online by electron transfer dissociation (ETD) tandem mass spectrometry (MS/MS).

136 int HR-HRPF, electron-transfer dissociation (ETD) tandem MS (MS/MS) acquisition, measurement of effec

137 ly developed electron-transfer dissociation (ETD) technique.

138 ive top-down electron transfer dissociation (ETD) techniques are employed to study AS interaction wit

139 on (PQD) and electron transfer dissociation (ETD) techniques for confident and quantitative identific

140 loped method electron transfer dissociation (ETD) to characterize the human ES cell phosphoproteome.

141 ond, we used electron transfer dissociation (ETD) to partially dissociate disulfide bonds followed by

142 Electron transfer dissociation (ETD) was employed to fragment the heavily modified long

143 Electron-transfer dissociation (ETD) was then used to pinpoint the 12 occupied O-glycosy

144 C-MS/MS with electron transfer dissociation (ETD) was used to identify proteins from specific locatio

145 Electron transfer dissociation (ETD) was used to sequence bis-arylhydrazone (BAH)-cross-

146 rmation from electron-transfer dissociation (ETD) were observed, suggesting the utility of this metho

147 on (CID) and electron transfer dissociation (ETD) with hybrid quadrupole time-of-flight instruments a

148 achieved by electron-transfer dissociation (ETD) with increased supplemental activation, without los

149 mentation of electron transfer dissociation (ETD) with online CZE separations for top-down proteomics

150 or augmented electron transfer dissociation (ETD) yields.

151 on (CID) and electron-transfer dissociation (ETD)).

152 s our use of electron transfer dissociation (ETD), a mass spectrometric fragmentation technique that

153 Electron transfer dissociation (ETD), a technique that provides efficient fragmentation

154 ation (HCD), electron transfer dissociation (ETD), and electron capture dissociation (ECD).

155 ation (HCD), electron-transfer dissociation (ETD), and electron-transfer combined with higher-energy

156 e CID (HCD), electron-transfer dissociation (ETD), and the combinations ETciD and EThcD.

157 e CID (HCD), electron-transfer dissociation (ETD), ETciD, and EThcD.

158 tra, such as Electron Transfer Dissociation (ETD), Higher-energy Collisional Dissociation (HCD) spect

159 ion (CAD) or electron transfer dissociation (ETD), respectively.

160 line with an electron transfer dissociation (ETD)-enabled hybrid Orbitrap Fourier transform mass spec

161 ted a hybrid electron-transfer dissociation (ETD)-HCD acquisition protocol and developed a novel data

162 followed by electron transfer dissociation (ETD)-MS(2) upon detection of glycan-specific oxonium is

163 D-MS/MS) and electron transfer dissociation (ETD)-MS/MS of intercross-linked peptides (two peptides c

164 first use of electron transfer dissociation (ETD)-produced diagnostic fragment ions to probe the comp

165 on (CID) and electron transfer dissociation (ETD).

166 enabled with electron transfer dissociation (ETD).

167 on (ESI) for electron-transfer dissociation (ETD).

168 (CID-MS(2)), electron-transfer dissociation (ETD-MS(2)), and CID of an isolated product ion derived f

169 d by precursor molecular weight and top-down ETD and HCD analysis of the reduced protein.

170 Native top-down ETD on the protein-ligand complexes shows how the differ

171 nd comparable sequence coverage for top-down ETD with orbitrap mass analyzers.

172 ns remain localized on basic residues during ETD but easily mobilize along the backbone during collis

173 electron capture/transfer dissociation (ECD, ETD) (c/z fragments), suggesting substantial contributio

174 ctron capture and transfer dissociation (ECD/ETD or ExD) mass spectrometry (MS) employed for peptide

175 ociation/electron transfer dissociation (ECD/ETD) product ions based on their number of hydrogen plus

176 electron capture/transfer dissociation (ECD/ETD).

177 ndergo dissociation analogous to that in ECD/ETD.

178 in the companion article, the number of ECD/ETD product ion amino acid compositions as a function of

179 discrimination between N- and C-terminal ECD/ETD peptides.

180 instruments not originally equipped with ECD/ETD capabilities.

181 e residues, which were overcome by employing ETD fragmentation.

182 ), a recently introduced method for enhanced ETD fragmentation, provides useful performance with CZE

183 In fact, ETD can be performed with optimal efficiency in nearly t

184 radical anion of fluoranthene (m/z 202) for ETD and the closed-shell anion resulting from H atom att

185 discrete ion storage and reaction steps for ETD experiments and no discrete ion storage step and fre

186 ce of UniNovo is superior to other tools for ETD spectra and superior or comparable with others for C

187 m/z) reagent anions, it is desirable to form ETD reagents via means other than those that require rea

188 CID of an isolated product ion derived from ETD (MS(3)) has been used to characterize disulfide-link

189 dual ESI sources that were used to generate ETD reagent ions, this source separates the emitters in

190 degludec/liraglutide vs 1.8 kg for glargine; ETD, -3.20 kg [95% CI, -3.77 to -2.64],P < .001) and few

191 rits of our method in analyzing glycopeptide ETD data.

192 is necessary for interpreting O-glycopeptide ETD spectra in order to expedite the analysis workflow.

193 tested types of spectra (including CID, HCD, ETD, CID/ETD and HCD/ETD spectra of trypsin, LysC or Asp

194 ra (including CID, HCD, ETD, CID/ETD and HCD/ETD spectra of trypsin, LysC or AspN digested peptides).

195 esponsible for the observed differences (HDX-ETD).

196 rk highlight the analytical potential of HDX-ETD combined with functional assays to guide protein eng

197 Gas-phase HDX-ETD experiments on ubiquitin ions ionized from both dena

198 identified 50% more peptides than ETD; (ii) ETD resulted in approximately 20% increase in amino acid

199 Mate was used to reinfuse samples to improve ETD and HCD spectra of glycopeptides.

200 (ET) product species ([M + 2H]+*) to improve ETD efficiency for doubly protonated peptides (ETcaD).

201 erred over peptide backbone fragmentation in ETD.

202 died the fragmentation of O-glycopeptides in ETD and found useful rules that facilitate their identif

203 ince the glycan side chain remains intact in ETD, and the glycosylation site can be localized on the

204 The relative high abundance ions observed in ETD provided strong evidence for the linked peptide info

205 work with any search engine that interprets ETD data of N-linked glycopeptides.

206 ciation (ETD) reactions, i.e., activated ion ETD (AI-ETD), significantly increases dissociation effic

207 this method for improving transmission mode ETD performance for relatively low charge states of pept

208 r dissociation-selected reaction monitoring (ETD-SRM) was developed to investigate isoAsp sites in MU

209 ort that negative ion CAD, EDD, and negative ETD (NETD) result in sulfonate retention mainly at highe

210 On average, 58% of the observed ETD product ion abundance was accounted for by fragment

211 re, we present a study on the application of ETD for analysis of phospholysine (pLys) peptides.

212 d highlight the different characteristics of ETD spectra of doubly charged precursors in comparison t

213 A detailed comparison of ETD and collision-induced dissociation (CID) modes showe

214 data, due to the complexity and diversity of ETD mass spectra compared to CID counterparts.

215 e to construct and enables implementation of ETD on any instrument without modification to footprint.

216 r results suggest that the implementation of ETD on sensitive, high-resolution, and high-mass accurac

217 been studied for decades, the intricacies of ETD-based fragmentation have not yet been firmly establi

218 Because of the nature of ETD fragmentation MS experiments were performed without

219 Upon optimization of ETD and product ion transfer parameters, results show th

220 n to the preferred fragmentation pathways of ETD, the TMT-127 and -129 reagents were recently modifie

221 he parameters governing the reaction rate of ETD are examined experimentally.

222 h that takes advantage of the specificity of ETD and the scalability of tandem MS, and the predictive

223 Here, we evaluated the use of ETD for a global phosphoproteome analysis.

224 expanded experimental options for the use of ETD.

225 Here we demonstrate the utility of ETD in high-throughput top-down proteomics using soluble

226 oughput glycopeptide identification based on ETD data, due to the complexity and diversity of ETD mas

227 ch was greater than by using CAD (38,293) or ETD (39,507) alone.

228 Compared with the Ascore using either CAD or ETD, the Cscore identified up to 88% more phosphorylatio

229 Typical ECD or ETD backbone fragmentations are completely inhibited in

230 ethods (electron capture or transfer, ECD or ETD) have distinctive chemical compositions from other c

231 upts the normal sequence of events in ECD or ETD, leading to backbone fragmentation by forming a stab

232 e tags generated either by low-energy HCD or ETD activation along with the intact protein mass inform

233 her fragmentation spectra compared to HCD or ETD alone, increasing protein sequence coverage, and the

234 -type ions observed when compared to UVPD or ETD alone, as well as generating a more balanced distrib

235 oving peptide identifications over all other ETD methods, making it a valuable new tool for hybrid fr

236 eptide fragmentation and identification over ETD and other supplemental activation methods.

237 verage of 89%-a significant improvement over ETD (63%) and ion trap CAD (77%).

238 type product ions for all charge states over ETD alone, boosting product ion yield by nearly 4-fold f

239 Overall, ETD is an excellent method for localization of phosphory

240 n to the original HCD-product dependent (pd)-ETD function.

241 te a drastic reduction in the time taken per ETD event.

242 ained ETD and ECD models are able to predict ETD and ECD spectra with reasonable accuracy in ion inte

243 In such cases, good quality ETD data may indicate the discrepancy, and will also dis

244 mmodate electron-transfer ion/ion reactions (ETD) for peptide and protein characterization.

245 e other hand, homologous murine rotaviruses (ETD or EHP) or the heterologous simian rotavirus (rhesus

246 and PTM localization by combining sequential ETD and HCD fragmentation in a single fragmentation even

247 mass spectrometers, ExD, more specifically, ETD MS has received particular interest in life science

248 e than triples identifications from standard ETD experiments and outperforms ETcaD and EThcD as well.

249 at (i) CID identified 50% more peptides than ETD; (ii) ETD resulted in approximately 20% increase in

250 From these experiments, we conclude that ETD identifies a larger number of unique phosphopeptides

251 Although our data indicate that ETD is superior to CID for phosphorylation analysis, the

252 A central finding is that ETD is approximately 2-fold more likely to cleave in sol

253 Here we show that ETD can be combined with fast gas-phase HDX in ND(3) gas

254 induced dissociation (CID) modes showed that ETD identified 60% more phosphopeptides than CID, with a

255 The ETD model was trained with more than 7000 ETD spectra, w

256 target glycopeptides, are scored against the ETD data, from which FDRs can be calculated accurately b

257 In the former approach, the ETD products are captured and stored in the linear ion t

258 In the latter approach, the ETD products pass through the linear ion trap and must b

259 ins, phosphate transfer reactions during the ETD process can be observed leading to ambiguous fragmen

260 ms the cleavage of the disulfides during the ETD process.

261 lation sites were clearly localized from the ETD fragmentation data.

262 hown to be obtained either directly from the ETD fragmentation of the precursors (disulfide-linked pe

263 ally disulfide-dissociated peptides from the ETD fragmentation was necessary for linkage assignment.

264 From the ETD/HCD mass spectra, 5162 and 6720 reliable SNLs and im

265 ectly from the charge-reduced species in the ETD fragmentation of the precursors.

266 In this paper we investigate the ETD fragmentation patterns of peptides labeled with 8-pl

267 Herein, we demonstrate the stability of the ETD anion population and the ability to identify several

268 he identities and relative abundances of the ETD products.

269 nfrared photon bombardment concurrent to the ETD reaction to mitigate nondissociative electron transf

270 tes input glycopeptide compositions with the ETD spectra, and assigns a score for each candidate.

271 The glycan remains intact through ETD, while the peptide backbone is cleaved, providing th

272 ion events as well as longer reaction times, ETD spectra require significantly more time to acquire t

273 n and alkylation of disulfide bonds prior to ETD analysis is evaluated by comparison of three derivat

274 ate for the CID product ions to give rise to ETD.

275 Similarly to ETD, proton transfer is found to compete with electron t

276 are preferentially cleaved when subjected to ETD.

277 The trained ETD and ECD models are able to predict ETD and ECD spect

278 ssessing an electron available for transfer (ETD).

279 cleaved over the glycosidic cleavages under ETD fragmentation.

280 Basically, pLys is stable under ETD conditions allowing an unambiguous assignment of the

281 and sequence coverage for all peptides upon ETD, including formation of diagnostic ions that allow c

282 product ions in multiple charge states upon ETD is minimized for the APTA-modified peptides.

283 We demonstrate that upon ETD, peptides labeled with 8-plex iTRAQ tags fragment to

284 , top-down proteomics can be performed using ETD in a linear ion trap mass spectrometer on a chromato

285 d demonstrate MS(2)-based quantitation using ETD.

286 ed peptide radical cations was studied using ETD.

287 Compared to those formed directly via ETD, the fragment ions were found to comprise increased

288 ertinent to spectra of peptides obtained via ETD reactions, have been used in the training.

289 the dynamic range of tandem MS analyses when ETD-based methods are compared to CID-based methods.

290 rison to the analogous MS(3) format in which ETD and UVPD were undertaken in separate segments of the

291 n-induced dissociation (CID) steps, in which ETD fragmentation preferentially induces cleavage of dis

292 efinitively extends the m/z range over which ETD can be effective for fragmentation of intact protein

293 With ETD, labile glycans were retained without any signs of c

294 rly double the identifications achieved with ETD alone but also that it outperforms the other availab

295 pping capabilities of the LTQ, combined with ETD, are demonstrated to provide single-residue resoluti

296 emonstrate that CZE is fully compatible with ETD as well as higher energy collisional dissociation (H

297 The online LC-MS with ETD methodology represents a powerful approach to aid in

298 (XICs) of cysteine-containing peptides with ETD analysis to produce an efficient assignment approach

299 ciation data into the database searches with ETD data may prove decisive in most cases.

300 ss tag (TMT) labeling and an LTQ Orbitrap XL ETD (electron transfer dissociation) hybrid mass spectro