ライフサイエンスコーパス: EcoRI

1 r stability and cleavage by an endonuclease (EcoRI).

2 three restriction enzymes (BsoBI, XhoI, and EcoRI).

3 ferent from that of the related endonuclease EcoRI.

4 igated and compared with a previous study on EcoRI.

5 ch greater dependence on osmotic stress than EcoRI.

6 sequence discrimination of substrate DNA by EcoRI.

7 .39 for HindIII, .04 for BamHI, and .02 for EcoRI.

8 ation of the circular SV40 genomic DNAs with EcoRI.

9 ontaining scaffold to M13mp7L2 linearized by EcoRI.

10 M.XmnI is loosely related to M.EcoRI.

11 eters for the cleavage reaction catalyzed by EcoRI.

12 adducts appeared to inactivate or sequester EcoRI.

13 -open were cleaved poorly, or not at all, by EcoRI.

14 M.EcoRI, a bacterial sequence-specific S-adenosyl-L-methio

15 is part of, or immediately adjacent to, the EcoRI active site and which is conserved in the distantl

16 nd PCR amplification was carried out with an EcoRI adaptor-specific primer labelled with fluorescent

17 In contrast, similar analysis of M.EcoRI, an adenine N6 DNA methyltransferase, shows an ave

18 n directing sequence-specific DNA binding by EcoRI and are also crucial in assisting site discriminat

19 y shift assays applied to the DNA binding of EcoRI and BamHI restriction endonucleases.

20 EcoRI and BamHI restriction fragment patterns indicated

21 Molecular Dynamics simulations on DNA-EcoRI and DNA-EcoRV complexes suggest that the DNA withi

22 otected supercoiled plasmid from cleavage by EcoRI and DraI enzymes at their respective restriction s

23 BamHI, EcoRI and EcoRV endonucleases all have different context

24 ctive ecoRVR gene between the cloning sites (EcoRI and HindII or NotI) permits the positive selection

25 We also report EcoRI and HindIII polymorphisms that may prove to be use

26 project, two whole-genome restriction maps (EcoRI and HindIII) of R. sphaeroides strain 2.4.1 were c

27 n with the restriction enzymes BamHI, BglII, EcoRI and KpnI increased the efficiency of linearized pl

28 R products were then digested with PvuII and EcoRI and ligated into a vector which had this same regi

29 terize the cognate and star site activity of EcoRI and MfeI and demonstrate genome-wide decreases in

30 ective primers were examined, and one, using EcoRI and MseI with additional selective TC bases on the

31 enomic DNAs were digested with endonucleases EcoRI and MseI, site-specific adaptors were ligated, and

32 n of about 4-fold resulted from catalysis by EcoRI and other proteins.

33 tivity by type II restriction enzymes BamHI, EcoRI and SalI, and inhibition was confirmed through the

34 th studies of more orthodox enzymes, such as EcoRI and some other type II restriction enzymes.

35 AFLP marker genotyping, using the EcoRI and TaqI restriction enzymes, provided an effectiv

36 esidues identified, 11 are conserved between EcoRI and the isoschizomer RsrI (which shares 50% identi

37 classes of DNA sites: specific, miscognate (EcoRI*) and non-specific.

38 that EcoRV bends DNA sharply, whereas BamHI, EcoRI, and DNaseI do not.

39 vage by HindIII was enhanced, whereas BamHI, EcoRI, and DNaseI were largely unaffected.

40 sing different restriction enzymes (HindIII, EcoRI, and MboI, respectively), were evaluated with a se

41 s digested with the restriction endonuclease EcoRI, and restriction fragment length polymorphisms wer

42 haromyces was digested with the endonuclease EcoRI, and the resultant fragments were separated by ele

43 currence of Class I SSRs in end-sequences of EcoRI- and HindIII-digested BAC clones was one SSR per 4

44 cket is achieved by biotinylating the mutant EcoRI at the mutation site.

45 on Tn5 mutagenesis and subcloned as a 2.0-kb EcoRI-AvaI fragment.

46 There is also a weaker correspondence with EcoRI, BamHI, and Cfr10I.

47 Restriction endonucleases such as EcoRI bind and cleave DNA with great specificity and rep

48 e show that at the cognate sequence (GAATTC) EcoRI binding releases about 70 fewer water molecules th

49 of a splay in the G(4)-C(9) base pair of the EcoRI binding site and a potential pocket of flexibility

50 The high affinity for the EcoRI binding site exhibited by this mutant endonuclease

51 erred by different flanking sequences on the EcoRI binding site.

52 creased end-to-end distance resulting from M.EcoRI binding, mediated by a mechanism novel for DNA met

53 cation concentration dependence of K(A) for EcoRI binding.

54 how a prototypical restriction endonuclease, EcoRI, binds to the DNA target sequence--GAATTC--in the

55 t with levels of restriction site avoidance, EcoRI, but not EcoRV, cleaves self-DNA at a measurable r

56 on of an exogenous restriction endonuclease, EcoRI, but not to UV irradiation.

57 te that the site-specific restriction enzyme EcoRI can be conjugated to 20-nm fluorescent nanoparticl

58 absence of Cu(2+), the Mg(2+)-dependence of EcoRI catalysis shows positive cooperativity, which woul

59 In contrast, EcoRI caused prolonged cell-cycle arrest of recombinatio

60 n this mutant (SK1) was carried on a 10.6-kb EcoRI chromosomal DNA fragment.

61 (1344 bp) genes were identified on an 8.2 kb EcoRI chromosomal fragment.

62 Sequence analysis of a 6.7-kilobase pair EcoRI-ClaI genomic clone revealed a single open reading

63 restriction endonuclease analysis (REA) with EcoRI clustered the U.S. and European isolates into two

64 EcoRI complexation with nonspecific DNA releases substan

65 of DNA adducts and the crystal structure of EcoRI complexed to substrate suggest a model to explain

66 the absence of cofactor magnesium ions, the EcoRI conjugates bind to specific sequences on double-st

67 rence in hydration changes by both BamHI and EcoRI could be attributed to these dissimilar secondary

68 When HindIII-X and EcoRI-D primer sets were used, CMV DNA from PBLs was a m

69 also subjected to Southern blot analysis of EcoRI-digested genomic DNA using the same full-length HT

70 otides were used to probe a Southern blot of EcoRI-digested HI689 genomic DNA.

71 of P1 cleavage sites in pBR322, achieved by EcoRI digestion after the original P1 attack, showed an

72 EcoRI digestion of the purified lambda DNA released a 5.

73 (8.2 kb and 10 kb respectively) generated by EcoRI digestion of total plasmid DNA.

74 with 5-azadeoxycytosine restored the normal EcoRI digestion pattern of wt1 in these cells indicating

75 ggesting that direct transfer contributes to EcoRI dissociation.

76 viously characterized specificity-enhanced M.EcoRI DNA adenine methyltransferase mutant suggest a clo

77 ctional gene was localized to a 2.15-kb SacI-EcoRI DNA fragment containing an open reading frame of 5

78 A cloned 6.0-kb BclI-EcoRI DNA fragment expresses a 120-kDa B. henselae prote

79 nas sp. strain ADP cosmid library as a 25-kb EcoRI DNA fragment in Escherichia coli.

80 The target adenine for EcoRI DNA methyltransferase (GAATTC) was replaced with 2

81 ingle site within the sequence recognized by EcoRI DNA methyltransferase (GAATTC).

82 We describe the characterization of an EcoRI DNA methyltransferase mutant in which histidine 23

83 ), k(off), and k(on)) was determined for the EcoRI DNA methyltransferase under noncatalytic condition

84 EcoRI DNA methyltransferase was previously shown to bend

85 rgetics and kinetics of base flipping by the EcoRI DNA methyltransferase were investigated by two met

86 Our investigation focuses on the EcoRI DNA methyltransferase which transfers a methyl gro

87 ding and base flipping were examined for the EcoRI DNA methyltransferase.

88 itatively rank the stability of bonds in the EcoRI-DNA complex.

89 We find that the dissociation rate of EcoRI-DNA-specific complexes at 80 mM NaCl depends on th

90 ith recent crystal and NMR structures of the EcoRI dodecamer, where an overall bend of seven degrees

91 ributable, at least in part, to diffusion of EcoRI(E111Q) away from its recognition site.

92 We show that a protein roadblock (EcoRI(E111Q), a hydrolytically defective form of EcoRI e

93 ead or head-to-tail orientations, as well as EcoRI(E111Q), lac repressor and even nucleosomes.

94 and SgrAI) and six one-site enzymes (BamHI, EcoRI, EcoRV, HaeIII, HindIII, and DNaseI).

95 okI, BglI, BglII, PvuII, SfiI, BssSI, BsoBI, EcoRI, EcoRV, MspI, and HinP1I were subjected to oxidizi

96 inding constants of the restriction nuclease EcoRI enable us to determine the diffusion rate of nonsp

97 Two mutants of the EcoRI endonuclease (R200K and E144C) predominantly nick

98 The contact between EcoRI endonuclease and the "primary clamp" phosphate of

99 the dissociation rate of specifically bound EcoRI endonuclease and the ratio of non-specific and spe

100 The N-termini of EcoRI endonuclease are essential for tight binding and c

101 rameters governing cleavage of pBR322 DNA by EcoRI endonuclease are highly sensitive to ionic environ

102 Analysis of EcoRI endonuclease expression in vivo revealed that, in

103 EcoRI endonuclease has two tryptophans at positions 104

104 2+) coordinates to histidine residues in the EcoRI endonuclease homodimer bound to its specific DNA r

105 Galactose-induced expression of EcoRI endonuclease in rad50, mre11, or xrs2 mutants, whi

106 The EcoRI endonuclease is an important recombinant DNA tool

107 The N-terminal region of EcoRI endonuclease is essential for cleavage yet is invi

108 We have isolated temperature-sensitive (TS) EcoRI endonuclease mutants (R56Q, G78D, P90S, V97I, R105

109 Expression of the EcoRI endonuclease mutants in the absence of the EcoRI m

110 A direct competition experiment with the EcoRI endonuclease shows the methyltransferase to be sli

111 the atomic force microscope (AFM), a mutant EcoRI endonuclease site-specifically bound to DNA.

112 , we have exploited "promiscuous" mutants of EcoRI endonuclease to study the detailed mechanism by wh

113 A single-step purification of EcoRI endonuclease using a sequence-specific DNA column

114 We have studied the interaction of EcoRI endonuclease with oligonucleotides containing GAAT

115 I(E111Q), a hydrolytically defective form of EcoRI endonuclease) placed on the helix between the two

116 usion proteins with staphylococcal nuclease, EcoRI endonuclease, beta-globin, and luciferase.

117 late Tel1 activation after expression of the EcoRI endonuclease, which generates "clean" DNA ends.

118 uence, and no change in energy transfer with EcoRI endonuclease, which leaves this sequence unbent.

119 ion X-ray crystal structure of the wild-type EcoRI endonuclease-DNA complex revealed that: (1) the TS

120 ncreased recombination and suppressed HO and EcoRI endonuclease-induced killing of rad50 strains.

121 Promiscuous mutant EcoRI endonucleases produce lethal to sublethal effects

122 s method uses cellular DNA digested with the EcoRI enzyme and the restriction fragment length polymor

123 gene and a universal primer pair followed by EcoRI enzyme digestion.

124 1A gene is included entirely within a 6.4-kb EcoRI fragment and comprises two coding exons separated

125 here the cloning and mapping of this 21.5 kb EcoRI fragment and it was shown to complement each of th

126 Sequencing of this EcoRI fragment confirmed that HGPRT and XPRT were organi

127 A 6.8-kb EcoRI fragment containing all but the 5' end of cfpA was

128 Southern hybridizations to identify a 4.6-kb EcoRI fragment containing the complete xynA gene.

129 The EcoRI fragment contains 13 kb of DNA that is specific to

130 7% sensitivity with primer pairs directed to EcoRI fragment D, 32% sensitivity with primer pairs dire

131 A 3.1-kb HindIII-EcoRI fragment found in both cosmids was shown to fully

132 6.5' promoter, have been subcloned on a 20kb EcoRI fragment from Kohara phage 19D1.

133 the 4.29 kb SspI fragment and an overlapping EcoRI fragment from one end of the inverted repeat, whil

134 e resulting phagemids revealed that a 0.5-kb EcoRI fragment hybridized with the F11 probe.

135 l 11p15.5 breakpoint which disrupts a 7.8 kb EcoRI fragment in all three of the delta t(X;11) chromos

136 Cloning of the 0.5-kb EcoRI fragment into the E. coli-streptococcal insertion

137 These results indicate that the 0.5-kb EcoRI fragment is part of an adhesin-relevant locus that

138 The 10 kb EcoRI fragment localized to lp28-1 and was subsequently

139 The 1.0 kb EcoRI fragment of IFG elements codes for the 3' half of

140 d from a 5.8-kb BamHI fragment and an 8.0-kb EcoRI fragment of strain J45 genomic DNA.

141 A 4074-bp EcoRI fragment of Streptococcus salivarius ssp. thermoph

142 e TnphoA insertions were mapped to a 21.5 kb EcoRI fragment of the O139 chromosome.

143 Southern blot hybridization of a 827-bp 3' EcoRI fragment of the TAL-H cDNA to human-mouse somatic

144 sfection of plasmids containing the EK or JK EcoRI fragment or a 3-kb plasmid with the UL34.5 gene of

145 fragment revealed that the presence of this EcoRI fragment resulted from an inability of this enzyme

146 y(dG-dC) polynucleotides and to a 400-bp DNA EcoRI fragment resulted in a shift in the fragment size

147 Further subcloning of a 148 bp BamHI/EcoRI fragment resulted in the strongest in vitro DNA re

148 This sequence contains a 55-kb EcoRI fragment that is also present in all but four deri

149 nd 5590 possessed insertions within a 5.0 kb EcoRI fragment that is not contiguous with the exoenzyme

150 Six mini-Tn 10 insertions in the 3.7 kb EcoRI fragment were recombined into the L. pneumophila c

151 ural gene resides within a 7.4-kilobase SalI-EcoRI fragment with four exons corresponding to amino ac

152 cted in vitro by deleting an internal 1.4-kb EcoRI fragment, did not show blue-staining sectors.

153 d with HGPRT within a 4.3-kilobase pair (kb) EcoRI fragment, implying that the two genes arose as a r

154 c endogenous MCF virus env-containing 4.6-kb EcoRI fragment.

155 tural gene from a lambda library as a 5.1-kb EcoRI fragment.

156 mutants with a plasmid containing the 3.7 kb EcoRI fragment.

157 We placed the exons on genomic EcoRI fragments and identified their flanking intronic s

158 EcoRI fragments containing Tn5 flanking sequences from t

159 ide probe.A Lambda ZAP II library containing EcoRI fragments of L. kirschneri DNA was screened, and a

160 A Lambda-Zap II library containing EcoRI fragments of Leptospira kirschneri DNA was screene

161 A Lambda-Zap II library containing EcoRI fragments of Leptospira kirschneri DNA was screene

162 The Type II restriction enzymes (e.g. EcoRI) gave rise to recombinant DNA technology that has

163 A 3.7 kb EcoRI genomic fragment containing the 700 bp DD-PCR prod

164 EcoRI genomic fragments containing ERG3 from the Dar-1 a

165 This was accomplished using EcoRI (Gln-111), a mutant of the restriction enzyme that

166 have been mapped in the DNA sequence of the EcoRI-H and -Dhet fragments of B95-8 Epstein-Barr virus.

167 8 to 20 bands, when hybridized to EcoRI- or EcoRI-HaeIII-digested DNA of independent C. tropicalis i

168 Interestingly, M.EcoRI has an intercalation motif observed in the FPG DNA

169 ivity with engineered high-fidelity variants EcoRI-HF and MfeI-HF, as well as quantify the influence

170 rent from those of ESRVs upon digestion with EcoRI, HindIII, NdeI, KpnI, and ScaI.

171 anogen bromide-activated Sepharose 4B) binds EcoRI in the absence of Mg2+ and elutes when Mg2+ is app

172 positive cooperativity, which would enhance EcoRI inactivation of foreign DNA by irreparable double-

173 exon 3 of HPRT: The enzymes BamHI, BglII and EcoRI increased the illegitimate integration efficiency

174 ly, rad52 mutants were not more sensitive to EcoRI-induced cell killing than wild-type strains.

175 Continuous EcoRI-induced scission of chromosomal DNA blocked the gr

176 ficient for EcoRI of 3 x 10(4) bp(2) s(-)(1) EcoRI is able to diffuse approximately 150 bp, on averag

177 expectedly, Mg(2+)-catalyzed DNA cleavage by EcoRI is profoundly inhibited by Cu(2+) binding at these

178 This distortion of DNA conformation by M.EcoRI is shown to be important for sequence-specific bin

179 lthough the method described is specific for EcoRI, it can be readily modified for the purification o

180 gated: seven enzymes with a single cut site (EcoRI, KpnI, NdeI, NotI, NruI, SmaI, XbaI), two enzymes

181 -bp G217B yps 21:E-9 probe or 512-bp HindIII-EcoRI-labelled Downs yps21:E-9).

182 nts were generated by partial digestion with EcoRI (library segments 1--4: 24-fold) and MboI (segment

183 firomycins 6 and 13 efficiently cross-linked EcoRI-linearized pBR322 DNA upon addition of Et3P.

184 EcoRI mapping of a dense set of overlapping clones valid

185 NotI and EcoRI mapping of the overlapping cosmids, hybridization

186 erichia coli DNA despite the presence of the EcoRI methylase.

187 I endonuclease mutants in the absence of the EcoRI methyltransferase induces the SOS DNA repair respo

188 Some clustering of the EcoRI/MseI AFLP markers was observed, possibly in centro

189 are AFLP products generated from either the EcoRI/MseI or PstI/MseI enzyme combinations.

190 We utilized both EcoRI/MseI- and EcoRI/PstI-digested genomic DNA to gener

191 h a bending-impaired, enhanced-specificity M.EcoRI mutant show minimal differences with the cognate D

192 es that is not digested with enzymes such as EcoRI, NlaIII, and SphI.

193 solution and solid-state NMR studies of the EcoRI nuclease target sequence, and solid-state NMR stud

194 We calculate a diffusion coefficient for EcoRI of 3 x 10(4) bp(2) s(-)(1) EcoRI is able to diffus

195 t and control DNA samples were digested with EcoRI or PstI and Southern-hybridized with the DMP1, DMP

196 MLST was more discriminatory than EcoRI or PvuII ribotyping and provided subtype data with

197 containing 8 to 20 bands, when hybridized to EcoRI- or EcoRI-HaeIII-digested DNA of independent C. tr

198 Binding preference for EcoRI* over non-specific DNA is also improved.

199 Based on EcoRI plasmid profiles (PP), two types, PP-11 and PP-13,

200 d with restriction enzymes ApaI plus TaqI or EcoRI plus MseI.

201 The presence of the EcoRI polymorphic site results in a 3.7-kb band, and its

202 FAFLP with an unlabelled nonselective EcoRI primer (Eco+0) and a labelled selective MseI prime

203 The M.EcoRI protein sequence is poorly accommodated into well

204 terns produced by three restriction enzymes, EcoRI, PstI, and HindIII.

205 We utilized both EcoRI/MseI- and EcoRI/PstI-digested genomic DNA to generate AFLP bands a

206 ups, conserved in the active sites of EcoRV, EcoRI, PvuII, and BamHI endonucleases, suggests that lig

207 Of the 12 resultant EcoRI-PvuII combination types, only 4 contained multiple

208 both association and catalytic phases of the EcoRI reaction, acting to change the specificity of the

209 nced by using a detector probe containing an EcoRI recognition sequence at its 5'-end that is not hom

210 The presence of M(1)dG in the EcoRI recognition sequence impaired the ability of the e

211 as homologous templates, for insertion of an EcoRI recognition site at the RIF1 locus and introductio

212 double (pGEM-luc and pSV-beta-galactosidase) EcoRI recognition sites were imaged, and the bound enzym

213 tions of the local, internal dynamics in the EcoRI restriction binding site, -GAATTC- induced by cyti

214 created a fluorescent marker using a mutant EcoRI restriction endonuclease (K249C) that enables prol

215 We employed the EcoRI restriction endonuclease as a model for the intera

216 dated by confirming that DNA cleavage by the EcoRI restriction endonuclease causes inversion of confi

217 Purification of EcoRI restriction endonuclease to apparent homogeneity w

218 kb) bound to a slide surface was digested by EcoRI restriction endonuclease, and the resulting restri

219 Using EcoRI restriction endonuclease, we have shown that the b

220 uence is known to suppress hydrolysis by the EcoRI restriction enzyme.

221 generated from phage lambda by intracellular EcoRI restriction following infection.

222 A 1 kb EcoRI restriction fragment cloned from a band visible in

223 ndIII and tissue plasminogen activator (TPA) EcoRI restriction fragment length polymorphisms-based ge

224 From one cosmid clone, a 7.5 kb EcoRI restriction fragment, which reacted strongly with

225 strain NC92 have been isolated on an 11.0-kb EcoRI restriction fragment.

226 sis revealed 5.5- and 2.4-kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile a

227 The rice-pathogenic strain contained 57 EcoRI restriction fragments that hybridize to the MGR586

228 on-specific probes to interrogate a detailed EcoRI restriction map of the region, ZNF genes were foun

229 n on a complete NotI and SalI, and a partial EcoRI restriction map.

230 with unpredicted HinfI RFLP, resulting in an EcoRI restriction site.

231 tural model for a DNA decamer containing the EcoRI restriction site.

232 genomic DNA in cells carrying the wild-type EcoRI restriction-modification system: (a) binding to Ec

233 ferent UGPase-cDNAs with BamHI, HindIII, and EcoRI revealed that at least two mRNA populations were p

234 The EcoRI ribotype diversity among bovine isolates (Simpson'

235 Some EcoRI ribotypes contained multiple serotypes; a subset o

236 x of discrimination [D] = 0.995) than either EcoRI ribotyping (D = 0.950) or AscI or ApaI single-enzy

237 EcoRI ribotyping differentiated 17 ribotypes, and DNA se

238 Our data show that (i) EcoRI ribotyping, combined with hylB and sodA sequencing

239 re characterized by serotyping and automated EcoRI ribotyping.

240 e contig, we determined the locations of the EcoRI, SacII, EagI, and NotI restriction sites in the cl

241 ciencies severalfold, while Asp718, HindIII, EcoRI, SalI, SmaI, HpaI, MscI, and SnaBI do not.

242 Southern blot hybridization of EcoRI/SalI-digested DNA with Cp3-13 provides a fingerpri

243 ted diffusion processes, that occur prior to EcoRI sequence recognition and subsequent to DNA cleavag

244 arental pBR322, which contains only a single EcoRI sequence, ruling out slow release of the enzyme fr

245 RFLP analysis of cellular DNA with EcoRI showed that all three strains of S. boulardii had

246 5 map unit) was cloned and inserted into the EcoRI site (1.0 map unit) in the late region of simian v

247 and 8862, respectively) and to knock out an EcoRI site (A to G at nt 8880).

248 ct-1 contained both genes joined at a unique EcoRI site and expressed both activities.

249 from an inability of this enzyme to cut at a EcoRI site in intron 1 of wt1.

250 overlapping primers that recognized a unique EcoRI site in the SV40 genome.

251 average structure and B-factors; within the EcoRI site itself, the rms deviation between the average

252 istance gene derived from pMCIpol A into the EcoRI site located in exon 2.

253 extends for 3012 nucleotides from the single EcoRI site to beyond the PstI site in the 3' long termin

254 hat occur once the enzyme has arrived at the EcoRI site, are essentially insensitive to ionic strengt

255 not express wt1 were also methylated at this EcoRI site.

256 Semi-quantitative estimates of rates of EcoRI* site cleavage in vivo, predicted using the bindin

257 When wild-type protein binds to an EcoRI* site, it forms structurally adapted complexes wit

258 the consequences of inflicting DNA nicks at EcoRI sites in vivo.

259 a pBR322 variant bearing two closely spaced EcoRI sites is governed by the same turnover number as h

260 iate looping of a segment of Ins that brings EcoRI sites located at -623 and +761 bp (relative to the

261 st artificial chromosomes (YACs) at specific EcoRI sites located within or adjacent to sequence-tagge

262 nce in uncloned DNA between the two terminal EcoRI sites of a YAC insert was approximately 1 Mbp larg

263 Thirteen pairs of EcoRI sites were targeted for double RARE cleavage in un

264 nts detected polymorphic HindIII, BamHI, and EcoRI sites.

265 from tighter binding and faster cleavage at EcoRI* sites (one incorrect base pair).

266 AAATTC EcoRI* sites are cleaved by A138T up to 170-fold faster

267 triction-modification system: (a) binding to EcoRI* sites is more probable than for wild-type enzyme

268 nity and elevated cleavage rate constants at EcoRI* sites makes double-strand cleavage of these sites

269 reater than those of the wild-type enzyme at EcoRI* sites.

270 mmary, these mutations provide insights into EcoRI structure and function, and complement previous ge

271 ication that these residues are critical for EcoRI structure and function.

272 The nucleotide sequence of a 7.0-kb EcoRV-EcoRI subclone was determined and found to contain open

273 For proteins such as EcoRI that locate their specific recognition site effici

274 The binding of EcoRI to cognate DNA was dominated by a dehydration of t

275 The binding of the restriction endonuclease EcoRI to DNA is exceptionally specific.

276 GAATTC, decreases the binding free energy of EcoRI to values nearly indistinguishable from nonspecifi

277 We measured the kinetics of DNA bending by M.EcoRI using DNA labeled at both 5'-ends and observed cha

278 e substrates (GAATTT, GGATTC) of wild type M.EcoRI using fluorescence resonance energy transfer and 2

279 cell cycling and lost viability rapidly when EcoRI was expressed.

280 r amplification (Ramp), S17C BamHI and K249C EcoRI, were conjugated to oligonucleotides, and immobili

281 enetically modified form of the endonuclease EcoRI which lacks cleavage activity but retains binding

282 etween complexes of the restriction nuclease EcoRI with nonspecific DNA and with the enzyme's recogni

283 Southern analysis revealed a 3.5 kb EcoRI wt1 fragment readily detectable in majority of mes

284 the cognate GAATTC site than does wild-type EcoRI yet displays relaxed specificity deriving from tig