ライフサイエンスコーパス: Edman degradation

1 e location of the cut sites through standard Edman degradation.

2 The attachment site was confirmed by Edman degradation.

3 phosphopeptides were subjected to sequential Edman degradation.

4 sis; of these, only 10 could be sequenced by Edman degradation.

5 mation whereby the N terminus was blocked to Edman degradation.

6 minus of the 38-kDa candidate was blocked to Edman degradation.

7 ated by reverse phase HPLC and identified by Edman degradation.

8 jected to N-terminal sequencing by automated Edman degradation.

9 , and labeled amino acids were identified by Edman degradation.

10 ads and analyzed using mass spectrometry and Edman degradation.

11 ons sequentially identified via conventional Edman degradation.

12 matography, mass spectrometric analysis, and Edman degradation.

13 , and labeled amino acids were identified by Edman degradation.

14 ate; the N-terminus of ZP2 was identified by Edman degradation.

15 annel domain and in the ACh binding sites by Edman degradation.

16 entified that were purified and sequenced by Edman degradation.

17 mammals and hence blocked from sequencing by Edman degradation.

18 ositive beads can be sequenced directly with Edman degradation.

19 same as the published sequence determined by Edman degradation.

20 ng from cleavage is blocked and resistant to Edman degradation.

21 c phosphopeptides, MALDI-TOF/MS analysis and Edman degradation.

22 te and its identity determined by sequential Edman degradation.

23 orthophthalaldehyde (OPA) prior to automated Edman degradation.

24 subjected to phosphoamino acid analysis and Edman degradation.

25 rminal pyroglutamyl residue that had blocked Edman degradation.

26 N-terminal Edman degradation amino acid sequence analysis showed th

27 n digestion, lectin affinity chromatography, Edman degradation amino acid sequence analysis, carbohyd

28 vitro, of which three could be identified by Edman degradation amino-acid sequencing.

29 Edman degradation analyses and co-migration of synthetic

30 Mass spectrometry and Edman degradation analyses show that pp alpha-GlcNAc-T2

31 is on an isolated glycated peptide utilizing Edman degradation analysis and MALDI-TOF/TOF mass spectr

32 Furthermore, Edman degradation analysis demonstrated that preferred s

33 Edman degradation analysis of a beta-arrestin 1 C-termin

34 Edman degradation analysis of aggregated proteins from t

35 Edman degradation analysis of collagen fiber degradation

36 no acid sequence was determined by automated Edman degradation analysis of proteolytic fragments of b

37 ains the original amino acid as confirmed by Edman degradation analysis, suggesting that the mRNA but

38 both Asp and isoAsp, which were assigned by Edman degradation and by isoAsp detection using protein

39 NH2-terminal sequences were determined by Edman degradation and compared with the genomic sequence

40 amino acid sequence has been established by Edman degradation and confirmed by PCR analysis.

41 -terminal residues of each mature protein by Edman degradation and confirmed the internal deletion in

42 ion of the protein, based on blank cycles in Edman degradation and corresponding serine or threonine

43 dioactive lysine residues were identified by Edman degradation and electrospray mass spectrometry fol

44 Edman degradation and liquid chromatography of tryptic p

45 igestion of reduced derivatives, followed by Edman degradation and mass analyses.

46 Edman degradation and mass spectrometric analyses of try

47 olated and individually sequenced by partial Edman degradation and mass spectrometry (PED-MS) to reve

48 ation on the purified fraction, by automatic Edman degradation and mass spectrometry analysis, identi

49 Edman degradation and mass spectrometry of V8 protease g

50 Sequencing of the toxin by Edman degradation and mass spectrometry revealed a 63 am

51 Analysis of COOH-terminal peptides with Edman degradation and mass spectrometry revealed an amid

52 Sequencing of peptide fragments from MtmB by Edman degradation and mass spectrometry revealed no chan

53 P and DPP, we performed a detailed analysis (Edman degradation and mass spectrometry) on selected try

54 Purified peptides were sequenced by Edman degradation and mass spectrometry, and the sequenc

55 ified and peptides were sequenced by partial Edman degradation and mass spectrometry.

56 KPPHQGPRPPRPRPKP) was determined by means of Edman degradation and mass spectrometry.

57 , and the peptides were sequenced by partial Edman degradation and matrix-assisted laser desorption i

58 s determined to be LLNEVMCYPLFDGGNIGLR using Edman degradation and matrix-assisted laser desorption/i

59 of the labeled fragment, followed by manual Edman degradation and radiochemical sequencing.

60 (3-BP) blocks the amino terminus of 4-OT to Edman degradation and results in the disappearance of th

61 ified proteins were determined by sequential Edman degradation and tandem mass spectrometry (MS/MS).

62 ENAL, where X represents unknown residue) by Edman degradation, and a full-length cDNA of the enzyme

63 plex was characterized by mass spectrometry, Edman degradation, and amino acid composition analyses.

64 ing a combination of phosphopeptide mapping, Edman degradation, and electrospray mass spectrometry, s

65 er band was purified, partially sequenced by Edman degradation, and found to match rat IgG heavy chai

66 ucts by high pressure liquid chromatography, Edman degradation, and mass spectrometry suggests that m

67 ells using a combination of peptide mapping, Edman degradation, and mass spectrometry.

68 ion analysis and exoglycosidase digestions), Edman degradation, and monosaccharide composition analys

69 conjunction with phosphoamino acid analysis, Edman degradation, and phosphopeptide mapping, demonstra

70 The final candidate was also blocked to Edman degradation; as before, a duplex probe was PCR amp

71 ions, with analysis by mass spectrometry and Edman degradation, both the heavy and light chains were

72 horesis (PAGE), Western immunoblot analysis, Edman degradation, circular dichroism spectroscopy, and

73 nal sequence of the MalH protein obtained by Edman degradation corresponded to the first 32 amino aci

74 Edman degradation demonstrated a phosphotyrosine in a YX

75 Characterization by mass spectrometry and Edman degradation demonstrated that both the N and C ter

76 sequence EEDRD and so forth as determined by Edman degradation, demonstrating signal peptidase proces

77 was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrom

78 become phosphorylated; sequence analysis by Edman degradation established that threonine 753 became

79 Edman degradation failed to reveal N-terminal sequences,

80 Edman degradation gave a single amino-terminal sequence

81 nt both by ion trap mass spectrometry and by Edman degradation identified Asn346 and Asn347 of alphaO

82 N-terminal sequencing by Edman degradation identified residue 16 as carboxymethyl

83 Partial proteolytic mapping and Edman degradation identified serine 257 as a major site

84 ides by automated microsequencing and manual Edman degradation identified the sites in Mnk1 as Thr(22

85 SDS-PAGE analysis followed by Edman degradation identified three cysteine-containing f

86 SDS/PAGE, mass spectrometry, and Edman degradation identified translation products corres

87 pt cysteine 211 was identified by sequential Edman degradation, implying that this was the amino acid

88 OOH-terminal fragment yielded no sequence by Edman degradation, indicating that parts of Ser-180 went

89 usly the frameshift through a combination of Edman degradation, MALDI-ToF mass fingerprint analysis o

90 ing peptides inside the bead using a partial Edman degradation/mass spectrometry method.

91 encoding peptides inside the bead by partial Edman degradation/mass spectrometry.

92 primary sequence was determined by combining Edman degradation/N-terminal sequencing and electrospray

93 Edman degradation of 32P-labeled protein identified seri

94 combination of tandem mass spectrometry and Edman degradation of a subfragment.

95 aphy and obtained its amino acid sequence by Edman degradation of a tryptic digest.

96 Solid-phase Edman degradation of I was used for positive identificat

97 sidues in each M4 segment were identified by Edman degradation of isolated tryptic fragments and gene

98 the agonist binding sites were identified by Edman degradation of isolated, labeled subunit fragments

99 Sequential cycles of Edman degradation of labeled receptor fragments identifi

100 Edman degradation of MTPv1 isolated from transfected cel

101 structure of CP2 was determined by automated Edman degradation of native CP2 and its proteolytic frag

102 ation along with data derived from automatic Edman degradation of peptide fragments, the SmCI sequenc

103 d Lys-92 and Lys-109 as acetylation sites by Edman degradation of peptides from [14C]acetate-labeled

104 Mapping, isolation, and Edman degradation of the ATP-protectable peptide from [3

105 Edman degradation of the F1 subunit yielded the sequence

106 Sequencing by Edman degradation of the intact polypeptides and mass sp

107 Phosphoamino acid analysis and manual Edman degradation of the isolated phosphopeptides enable

108 Edman degradation of the larger amelogenin ran for 42 cy

109 e determined for the N terminus by automated Edman degradation of the purified enzyme.

110 sequence of halocin S8 was obtained first by Edman degradation of the purified protein and verified f

111 Edman degradation of the purified protein determined the

112 attachment to the protein was determined by Edman degradation of the resulting peptide-DNA complex t

113 Amino acid analysis and Edman degradation of tryptic peptides proved that the co

114 lectrospray ionization-mass spectrometry and Edman-degradation of peptides derived from HNE-modified

115 nce analysis; in these cases, sequencing (by Edman degradation or by mass spectrometry) confirmed tha

116 were subjected to multiple cycles of partial Edman degradation (PED) by the treatment with a 15-30:1

117 rated by reversed-phase HPLC for analysis by Edman degradation peptide sequencing.

118 eling with [(32)P]H(3)PO(4), modified manual Edman degradation, phosphoamino acid analysis, endoprote

119 ns is important for amino acid sequencing by Edman degradation, protein identification by shotgun and

120 on of [(35)S]methionine residues released by Edman degradation reaction.

121 Edman degradation remains the primary method for determi

122 Sequencing by Edman degradation revealed a 21-residue peptide (GCRFCCN

123 Edman degradation revealed that each mutant protein was

124 Amino acid sequence analysis by Edman degradation revealed that it has 47 residues, with

125 e sequence analysis by mass spectrometry and Edman degradation revealed that RH70 is the previously r

126 Edman degradation revealed that Trp-6 and Trp-9 were cov

127 phosphoserine was defined by the N-terminal Edman degradation sequence analysis as being the fourth

128 amino acid composition analysis, N-terminal Edman degradation sequence analysis, and tandem mass spe

129 eins and confirmed MMP-dependent cleavage by Edman degradation sequence analysis.

130 s could not be identified by analysis of the Edman degradation sequencer product because the palmitoy

131 confirmed by mass spectrometry analysis and Edman degradation sequencing of proteolytic products gen

132 Edman degradation sequencing of radiolabeled cyanogen br

133 ture for these peptides was determined using Edman degradation sequencing, and their cystine pairing

134 h transmembrane domain), as proven by direct Edman degradation sequencing.

135 ing, deglycosylation, micropurification, and Edman degradation sequencing.

136 f a tryptic peptide by mass spectrometry and Edman degradation showed a cleavage after Val129.

137 teine 8, combined with mass spectrometry and Edman degradation, showed that disulfide bonds link cyst

138 rgan with [(14)C]halothane and determined by Edman degradation some of the photolabeled amino acids i

139 cing proteins, such as mass spectrometry and Edman degradation, suffer from short reads and lack sens

140 Edman degradation suggested that the radiolabeled phosph

141 Specialized Edman degradation techniques have completed the structur

142 sequence of the precursor, we determined by Edman degradation the N-terminal amino acid sequences of

143 hRs with [(3)H]epibatidine and identified by Edman degradation the photolabeled amino acids.

144 Following Edman degradation, three 17-mer oligodeoxyribonucleotide

145 ecreted prorenin was determined by automated Edman degradation to be Leu22 while the N-terminus of th

146 tide fragments, which were then sequenced by Edman degradation to determine the glycosylation sites.

147 was established, by mass spectroscopy and by Edman degradation, to be between a tryptophan at positio

148 An N-terminal block to Edman degradation was observed when any of five differen

149 Automated Edman degradation was used to obtain N-terminal and inte

150 Using a combination of mass spectrometry and Edman degradation, we mapped the cleavage sites and char

151 acid sequences of these proteins obtained by Edman degradation were compared with sequences from the

152 he wild-type or mutant peptides by automated Edman degradation were unsuccessful.

153 sequences from de novo mass spectrometry and Edman degradation with an expressed sequence tag library