ライフサイエンスコーパス: Ehrlichial

1 equence analysis of a 919-bp sequence of the ehrlichial 16S rRNA gene amplified by PCR from the blood

2 Granulocytic ehrlichial 16S rRNA gene DNAs from six dogs were amplifi

3 the majority of polyreactive IgM recognized ehrlichial Ag(s), including an immunodominant outer memb

4 An ehrlichial agent (Venezuelan dog Ehrlichia [VDE]) was is

5 olation and antigenic characterization of an ehrlichial agent from a dog in South America, as well as

6 The ehrlichial agent was isolated from the blood of experime

7 ood rats serve as reservoirs of granulocytic ehrlichial agents in certain areas of northern Californi

8 United States and tested against a panel of ehrlichial and rickettsial pathogens.

9 Ehrlichial antibodies were measured by IFA using Ehrlich

10 ell responses, (iii) enhanced secondary anti-ehrlichial antibody response, (iv) accelerated bacterial

11 ehrlichial burden, and cytokine responses to ehrlichial antigen in primary splenic cultures during th

12 is presently infected or has been exposed to ehrlichial antigens in the past.

13 hree dogs infected with VDE by using several ehrlichial antigens revealed that the antigenic profile

14 ibed because of similarity of titers to both ehrlichial antigens.

15 ny of these three cytokines, indicating that ehrlichial binding is required for IL-1beta mRNA express

16 These results indicate that ehrlichial binding to host cells is sufficient to inhibi

17 the infection, yet had significantly higher ehrlichial burden both in the blood and tissues than C57

18 sayed for hepatic histopathological lesions, ehrlichial burden, and cytokine responses to ehrlichial

19 e by regulating liver injury and controlling ehrlichial burden.

20 Ehrlichial cell division was initiated soon after, resul

21 protein, OMP-19, protects mice against fatal ehrlichial challenge infection, and we identified a CD4

22 d and SLP mice were protected against lethal ehrlichial challenge infection.

23 oduce only IgM, were protected against fatal ehrlichial challenge infection.

24 lls, contributed to protection against fatal ehrlichial challenge.

25 ition was HGE agent dose dependent, required ehrlichial contact with the host cells, and was reversib

26 during a febrile condition characteristic of ehrlichial disease.

27 Attempts to amplify and detect ehrlichial DNA from the limited tissues available (n = 4

28 PCR amplifications of ehrlichial DNA targeting the 16S rRNA gene, the variable

29 Ehrlichial DNA was identified in 17 (68%) of the 25 sero

30 ing a number of host cell processes, but the ehrlichial effector proteins involved are unknown.

31 that these reagents did not directly inhibit ehrlichial energy metabolism.

32 his is in contrast to the MSP2 homologues in ehrlichial genogroup I pathogens, Ehrlichia chaffeensis,

33 mplexity requires efficient use of the small ehrlichial genome.

34 Consistent with other ehrlichial glycoproteins, the gp19 protein exhibited a l

35 morula membrane is the interface between the ehrlichial growing environment and the host cytoplasm, M

36 anscription-PCR analysis, the mRNA levels of ehrlichial HSP70 and HSP60 were examined after temperatu

37 The ehrlichial HSP70 gene encoded a 637-amino-acid protein,

38 of E. coli dnaK was observed upstream of the ehrlichial HSP70 gene.

39 s promote immunopathology and defective anti-ehrlichial immunity, possibly via decreasing the protect

40 Similar to other reported ehrlichial immunoreactive glycoproteins, carbohydrate wa

41 These results suggest that ehrlichial inclusions are early endosomes which selectiv

42 After 6 h postinfection, 100% of the ehrlichial inclusions became TfR positive and the intens

43 nt Dishevelled induced lysosomal fusion with ehrlichial inclusions corresponding to p62 degradation a

44 Ehrlichial inclusions did not fuse with lysosomes, since

45 t colocalization of predominately SUMO1 with ehrlichial inclusions was observed.

46 Some ehrlichial inclusions were labeled positive with antibod

47 ed that up to 3 h postinfection, most of the ehrlichial inclusions were negative for TfRs.

48 The ehrlichial inclusions were slightly acidic, since they w

49 roteins and PLC-gamma2 were colocalized with ehrlichial inclusions, as determined by double-immunoflu

50 ocalization of tyrosine phosphoproteins with ehrlichial inclusions.

51 n TRP120-interacting host protein, PCGF5, to ehrlichial inclusions.

52 Ehrlichial infection also inhibited spleen IgG responses

53 nstrating that DCs play an important role in ehrlichial infection and immunity.

54 is inoculum size can influence the course of ehrlichial infection and should be regarded as important

55 fection (peritoneum) following lethal murine ehrlichial infection compared to innocuous ehrlichial in

56 The study indicated that persistent ehrlichial infection contributes to heterologous protect

57 In conclusion, protection against fatal ehrlichial infection correlates with strong expansion of

58 To test whether ehrlichial infection disrupts Janus kinase (Jak) and sig

59 Ehrlichial infection in dogs was defined as concurrence

60 Lethal ehrlichial infection in wild-type mice induced a decline

61 In this study, signaling events required for ehrlichial infection of human monocytic cell line THP-1

62 rabbits there, but the identity of the other ehrlichial infection remains undescribed.

63 p a tick vector transmission animal model of ehrlichial infection using a human pathogen, Ehrlichia m

64 ted in the reduction of TRP120-Ub, decreased ehrlichial infection, and reduced recruitment of a known

65 small molecules also significantly decreased ehrlichial infection, indicating that the Ub pathway is

66 In dogs that developed an ehrlichial infection, thrombocytopenia occurred by 28 da

67 ted with the intermediate doses developed an ehrlichial infection.

68 he two highest dose of E. canis developed an ehrlichial infection.

69 locks Wnt secretion, significantly decreased ehrlichial infection.

70 e ehrlichial infection compared to innocuous ehrlichial infection.

71 antibodies can protect SCID mice from fatal ehrlichial infection.

72 homologous and heterologous immunity during ehrlichial infection.

73 an animal model for the study of persistent ehrlichial infection.

74 leotide in THP-1 cells significantly blocked ehrlichial infection.

75 m immunopathological mechanisms initiated by ehrlichial infection.

76 standing of the mechanisms of persistence of ehrlichial infections in humans, domestic animals, and r

77 se of infection than previously reported for ehrlichial infections induced by intravenous inoculation

78 We conclude that ehrlichial infections may be as common as spotted fever

79 cent) were obtained from patients with fatal ehrlichial infections.

80 les from patients with other rickettsial and ehrlichial infections.

81 10(5) bacterial genomes/mouse, respectively) ehrlichial inocula.

82 hosphorylation is specific and essential for ehrlichial internalization, replication, and spreading i

83 dicating that the Ub pathway is critical for ehrlichial intracellular replication and survival.

84 rlichia spp. suggests that genes involved in ehrlichial iron acquisition have been subject to reducti

85 ong otherwise indistinguishable granulocytic ehrlichial isolates.

86 ng, and the distribution and quantity of the ehrlichial load by immunohistochemistry, polymerase chai

87 time, hematological changes, PCR detection, ehrlichial load, seroconversion time, and titer range we

88 s, resulted in significant reductions in the ehrlichial load.

89 ion of rickettsemia, and initial and maximal ehrlichial loads between the two routes of infection.

90 The highest ehrlichial loads were present between days 4 and 14 afte

91 logical grading in these organs and relative ehrlichial loads.

92 A gene encoding a 23.5-kDa ehrlichial morula membrane protein designated MmpA was c

93 LC3 colocalized with ehrlichial morulae on days 1, 2, and 3 postinfection, an

94 Ehrlichial myocarditis is an unusual finding in nonimmun

95 arge repertoire of the p28 genes in a single ehrlichial organism and antigenic diversity of the P28 a

96 Strain HF565, an ehrlichial organism closely related to E. chaffeensis is

97 Identical 16S rRNA gene sequences among ehrlichial organisms from dogs, ticks, and a human in th

98 ernalization and continuous proliferation of ehrlichial organisms or the production of ehrlichial pro

99 rne pathogen, one of several closely related ehrlichial organisms that cause disease in animals and h

100 We have shown that immunization with an ehrlichial outer membrane protein, OMP-19, protects mice

101 sis of these interactions and their roles in ehrlichial pathobiology are not well defined.

102 ctor alpha, Stat1, and CD48) associated with ehrlichial pathobiology were strongly upregulated during

103 n 2 (MSP2) and MSP3 of the persistent bovine ehrlichial pathogen Anaplasma marginale are immunodomina

104 Protective immunity against the ehrlichial pathogen Anaplasma marginale has been hypothe

105 Native major surface protein 1 (MSP1) of the ehrlichial pathogen Anaplasma marginale induces protecti

106 Anaplasma marginale is an ehrlichial pathogen of cattle that establishes lifelong

107 Anaplasma marginale, an intraerythrocytic ehrlichial pathogen of cattle, establishes persistent in

108 Anaplasma marginale is an ehrlichial pathogen of cattle, in the order Rickettsiale

109 Isolation of the ehrlichial pathogen was achieved by inoculating patient

110 cture of Anaplasma marginale, a genogroup II ehrlichial pathogen, in both an acute outbreak and in pe

111 o valid small animal model that uses a human ehrlichial pathogen.

112 Although specific associations between ehrlichial pathogenicity and genotype were not identifie

113 port the use of IL-12 coadsorbed with OMP of ehrlichial pathogens in alum to amplify both antibody an

114 Protective immunity against ehrlichial pathogens is believed to require induction of

115 Tick-borne ehrlichial pathogens of animals and humans require a mam

116 ocytes participate in protective immunity to ehrlichial pathogens through production of gamma interfe

117 of TRP activation of Wnt pathways to induce ehrlichial phagocytosis.

118 ocytes, which have a life span too short for ehrlichial proliferation.

119 ntified a 1884-bp open reading frame with an ehrlichial promoter.

120 re required for antiapoptotic signaling, but ehrlichial protein synthesis and/or proliferation is not

121 of ehrlichial organisms or the production of ehrlichial proteins are required for the up-regulation o

122 ar and antigenic characterization of a human ehrlichial species in South America.

123 Recent data for several ehrlichial species show differential expression of the O

124 nant outer membrane proteins (OMPs) in other ehrlichial species.

125 e effector proteins involved in facilitating ehrlichial survival in mononuclear phagocytes.

126 onents of PI3K-GSK3-mTOR signaling decreased ehrlichial survival.

127 pathway, and activation was induced by three ehrlichial tandem repeat protein (TRP) effectors, with T

128 Consistent with other ehrlichial TRPs, the TRPs identified in ehrlichial whole

129 Ehrlichial vacuoles did not colocalize with the lysosoma

130 ng early infection, autophagosomes fuse with ehrlichial vacuoles to form an amphisome indicated by th

131 ther ehrlichial TRPs, the TRPs identified in ehrlichial whole-cell lysates and the recombinant protei