ライフサイエンスコーパス: F plasmid

1 conjugative transfer of the Escherichia coli F plasmid.

2 structure of PsiB from the Escherichia coli F plasmid.

3 milar to the tra (conjugation) region of the F plasmid.

4 gation system related to that encoded by the F plasmid.

5 ested that this mechanism is confined to the F plasmid.

6 s the exclusion of T7 in cells harboring the F plasmid.

7 to part of the transfer (tra) operon of the F plasmid.

8 mids closely related to the Escherichia coli F plasmid.

9 netic elements like bacteriophage Mu and the F plasmid.

10 ell surface of Escherichia coli carrying the F plasmid.

11 opB protein involved in the equipartition of F plasmid.

12 nd the finO, traD, and repA sequences of the F-plasmid.

13 gly, just as seen for strains with lac on an F' plasmid.

14 equires that the lac operon be located on an F' plasmid.

15 am mutation in strain SM195 is carried on an F' plasmid.

16 y E. coli strains, 21 bacteriophage genomes, F plasmid and eight transposons.

17 ing features similar to those of TraA of the F plasmid and have shown that VirB2 is required for the

18 in, which is encoded by the Escherichia coli F plasmid and is involved in the partition of the single

19 the pCU1 relaxase is similar to that of the F plasmid and plasmid R388 relaxases.

20 ion group of six Tra proteins encoded by the F plasmid and required by F(+) cells to elaborate F pili

21 inimal origin region of the Escherichia coli F plasmid and the minimal origin of replication of the A

22 e pUTI89 plasmid has characteristics of both F plasmids and other known virulence plasmids.

23 In F-plasmid and related systems, the homologous protein ac

24 o be important for the stable inheritance of F-plasmids and the prophage form of bacteriophage P1.

25 the function of keeping a segregated pair of F plasmids apart while the cell septum is being formed.

26 , and Cuzin pioneered the development of the F plasmid as a model system to study replication control

27 The well-characterized F-plasmid-based CcdAB TA system is important for F-plasm

28 and the natural CcdB protein encoded by the F plasmid both inhibit bacterial growth by attacking DNA

29 nexpected parallels to the regulation of the F-plasmid CcdB activity by CcdA and further supports a c

30 Homologs of the F plasmid conjugation genes are physically located on th

31 patial patterns of gene transfer mediated by F plasmid conjugation in a colony of Escherichia coli gr

32 During F plasmid conjugation, however, the SOS response is supp

33 tructural and functional organization of the F plasmid conjugative coupling protein TraD by coimmunop

34 honates that are nanomolar inhibitors of the F plasmid conjugative relaxase in vitro.

35 Early in F plasmid conjugative transfer, the F relaxase, TraI, cl

36 An F-plasmid containing a ccd locus can, therefore, functio

37 From the measured number of F plasmid copies per cell it appears that each migrating

38 plasmid has a dual role in the partition of F plasmid copies to daughter cells prior to division.

39 to prevent the redundant entry of additional F plasmids during active growth of the host cells.

40 overexpression of SopB, an Escherichia coli F plasmid-encoded partition protein, led to silencing of

41 TraI (DNA helicase I) is an Escherichia coli F plasmid-encoded protein required for bacterial conjuga

42 , the SOS response is suppressed by PsiB, an F-plasmid-encoded protein that binds and sequesters free

43 , facilitating proper folding of a subset of F-plasmid-encoded proteins in the periplasm.

44 The F-plasmid-encoded TraI protein, also known as DNA helica

45 The F plasmid genes examined were finO, traD, traY, and repA

46 The relatively high rate of recombination in F-plasmid genes suggests that conjugational gene transfe

47 The SopB protein of the F plasmid has a dual role in the partition of F plasmid

48 for quantifying the transfer kinetics of the F plasmid in a population by enumerating the relative ab

49 s studies have demonstrated that a DeltatrbB F plasmid in Escherichia coli lacking DsbC(E.coli), its

50 rus (PRV) genome was constructed as a stable F plasmid in Escherichia coli.

51 gative pili such as the conjugative pilus of F plasmid in Escherichia coli.

52 mixture experiments we observe an excess of F plasmid in the early saturation phase that equilibrate

53 s reversion of a lac frameshift allele on an F' plasmid in Escherichia coli.

54 The implications of F-plasmid insertion into the viral genome with regard to

55 However, the F-plasmid insertion present in the viral gG locus was fo

56 The insertion of the F-plasmid into the HHV8 genome interrupts the ORF56 gene

57 ative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it

58 We have studied the interaction of the F plasmid killer protein CcdB with its intracellular tar

59 lowly dissociates from the 3'-end of cleaved F plasmid, likely a characteristic essential for plasmid

60 asmid-based CcdAB TA system is important for F-plasmid maintenance.

61 ally the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of mol

62 d the processing of the propilin TraA of the F plasmid now extends to E. coli.

63 epA, which map at dispersed positions on the F plasmid of E. coli.

64 s of F-pili (conjugative pili encoded by the F plasmid of Escherichia coli).

65 on plasmid QpH1 of Coxiella burnetii and the F plasmid of Escherichia coli, respectively, are shown t

66 ases, virtually identical) to those found in F plasmids of E. coli natural isolates.

67 One well-studied model is the F plasmid partition system, SopABC.

68 Here, we reconstituted the F-plasmid partition system using a DNA-carpeted flow cel

69 Here we demonstrate that the F-plasmid-partitioning protein SopA polymerizes into fil

70 A derivative of the F plasmid, pOX38-tra715, expresses the entire F tra oper

71 ion allow phage T7 to avoid exclusion by the F plasmid, presumably by protecting the cell from premat

72 The F plasmid PsiB protein inhibits all activities of the Re

73 Here we report a mutagenesis of the F plasmid relaxase gene traI using in-frame, 31-codon in

74 The F plasmid relaxase TraI (1,756 amino acids) is also a hi

75 protein on the formation and activity of the F plasmid relaxosome has been examined.

76 The conjugative transfer of bacterial F plasmids relies on TraM, a plasmid-encoded protein tha

77 equence of RepA is not homologous to that of F plasmid RepE, we found by using fold-recognition progr

78 the HHV8 complete genome onto a prokaryotic F-plasmid replicon which allows the propagation of the r

79 Segregation of the prototypical F plasmid requires the centromere-binding protein SopB,

80 dance of the virulence plasmid and all three F-plasmid sequences in subspecies I serovar Choleraesuis

81 on of the active partitioning systems of the F plasmid (sopABC) or RK2 (O(B1) incC korB) resulted in

82 binding of SopB to specific sites within the F plasmid sopC locus involves mainly the C-terminal regi

83 In the F plasmid system, two auxiliary proteins have roles in e

84 and a 5' to 3' DNA helicase that unwinds the F plasmid to provide the single-stranded DNA that is tra

85 the ability of TraR, encoded on the episomal F' plasmid, to upregulate the sigma(E) extracytoplasmic

86 The product of the Escherichia coli F plasmid traI gene is required for DNA transfer via bac

87 The F plasmid TraI protein (DNA helicase I) plays an essenti

88 Crystal structures of the F plasmid TraI relaxase domain, with and without bound s

89 TraI36, a domain of F plasmid TraI that contains relaxase activity, binds a

90 For F plasmid, TraI, a relaxase or nickase, binds a single p

91 F-pilin precursor, propilin, involves three F plasmid transfer products: TraA, the propilin precurso

92 onstant inoculation densities, we extract an F plasmid transfer rate of 5 x 10(-10) (cells/mL . min)(

93 antisense sRNA and the traJ mRNA to control F plasmid transfer.

94 erefore, two TraI molecules are required for F plasmid transfer.

95 We have examined the role of the F-plasmid TraV outer membrane lipoprotein in the assembl

96 ole of two internal cysteine residues of the F-plasmid TraV outer membrane lipoprotein.

97 We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo.

98 F plasmids use surface exclusion to prevent the redundan

99 The F' plasmid, which carries lac, contributes by stimulatin