ライフサイエンスコーパス: F. nucleatum

1 F. nucleatum activated p38 and c-Jun NH(2)-terminal kina

2 F. nucleatum activates IL-1beta processing through the N

3 F. nucleatum also promoted invasion of KB cells by other

4 F. nucleatum and C. rectus were also associated with EOP

5 F. nucleatum and S. cristatus coaggregate strongly via a

6 F. nucleatum binding to clinical adenocarcinomas correla

7 F. nucleatum biofilm coculture with OD-E model causes la

8 F. nucleatum cell wall and FAD-I induced hBD2 via TLR2.

9 F. nucleatum isolated from other parts of the body may o

10 F. nucleatum ssp. nucleatum and ssp. polymorphum signifi

11 F. nucleatum strains isolated from amniotic fluids and p

12 F. nucleatum treatment induced apoptosis of PBMCs and PM

13 F. nucleatum was first detected in the blood vessels in

14 F. nucleatum was systematically detected in samples with

15 tabase further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.

16 either Actinobacillus actinomycetemcomitans, F. nucleatum, or P. gingivalis.

17 In addition, F. nucleatum seems to be important for the development o

18 he IL-8 secreted from epithelial cells after F. nucleatum stimulation could be down-regulated by subs

19 All F. nucleatum strains stimulated significant increase in

20 trophils were significantly increased in all F. nucleatum groups compared with control (P <0.001).

21 Although F. nucleatum vincentii also reduced superoxide generatio

22 Although F. nucleatum vincentii also reduced superoxide generatio

23 mcomitans exhibited mutualism, and, although F. nucleatum was unable to grow with either of the other

24 It is highly conserved among F. nucleatum, Fusobacterium periodonticum, and Fusobacte

25 tial role for this enzyme in establishing an F. nucleatum intracellular niche.

26 ort the isolation and characterization of an F. nucleatum (ATCC 25586)-associated defensin inducer (F

27 we present the first characterization of an F. nucleatum Type Vd phospholipase class A1 autotranspor

28 colonization of A. actinomycetemcomitans and F. nucleatum/periodonticum was not statistically associa

29 P. gingivalis, A. actinomycetemcomitans, and F. nucleatum was found to be higher in healthy individua

30 physical interaction between C. albicans and F. nucleatum and for the first time revealed the identit

31 that the interaction between C. albicans and F. nucleatum leads to a mutual attenuation of virulence,

32 physical interaction between C. albicans and F. nucleatum was mediated by the carbohydrate components

33 the regulation of hBD-2 mRNA; TNF-alpha and F. nucleatum cell wall induced hBD-2 mRNA rapidly (2 to

34 etected in the matching AF, with E. coli and F. nucleatum as the most prevalent.

35 capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1.

36 ccharides isolated from Escherichia coli and F. nucleatum were poor stimulants of hBD-2, although the

37 In both E. coli and F. nucleatum, FadA exists in two forms, the intact pre-F

38 stainings were negative in both controls and F. nucleatum cocultures.

39 A. actinomycetemcomitans, E. corrodens, and F. nucleatum was determined using an immunoassay.

40 sequential challenge with P. gingivalis and F. nucleatum and vice versa were approximately identical

41 periodontitis induced by a P. gingivalis and F. nucleatum mixed infection, and also on the local host

42 Infection of mice with P. gingivalis and F. nucleatum strains elicited lesions of various sizes a

43 hages that were exposed to P. gingivalis and F. nucleatum, without impairing their viability.

44 with/without priming with P. gingivalis and F. nucleatum.

45 and F. nucleatum but not with S. oralis and F. nucleatum, indicating that P. gingivalis and S. orali

46 The aggregation of PBMCs and F. nucleatum T18 was inhibited by either L-arginine, L-l

47 Also, it grew with Veillonella sp. and F. nucleatum but not with S. oralis and F. nucleatum, in

48 ygen requirements (E. coli, A. viscosus, and F. nucleatum), as well as a model mammalian cell line (m

49 We suggest that A. aphrophilus, F. nucleatum, and S. intermedius are key pathogens for t

50 These miRNAs can enter bacteria, such as F. nucleatum and E. coli, specifically regulate bacteria

51 At F. nucleatum/P. gingivalis ratios of > or = 1:1, spreadi

52 Coaggregation-mediated interactions between F. nucleatum and other species facilitated the survival

53 The CoAg reaction between F. nucleatum and the Candida species involved a heat-lab

54 The CoAg reactions between F. nucleatum and the Candida species may be important in

55 Moreover, anti-FAD-I antibodies blocked F. nucleatum induction of hBD-2 by more than 80%.

56 l cavity, and the CoAg of C. dubliniensis by F. nucleatum when grown at 37 degrees C provides a rapid

57 iological pathways significantly impacted by F. nucleatum and S. gordonii included the mitogen-activa

58 However, hBD-2 induction by F. nucleatum was not blocked by pretreatment with two NF

59 NK partially blocked hBD-2 mRNA induction by F. nucleatum, and the combination of two inhibitors comp

60 hat T cell activities were also inhibited by F. nucleatum via Fap2.

61 BD-2 induction, and that hBD-2 regulation by F. nucleatum is via p38 and JNK, while phorbol ester ind

62 ls of CCL20 at 6 h, following stimulation by F. nucleatum cell wall (FnCW).

63 helial cell, as well as protein synthesis by F. nucleatum.

64 her bacteria linked to colorectal carcinoma, F. nucleatum does not exacerbate colitis, enteritis, or

65 Interestingly, in flow cells F. nucleatum and A. actinomycetemcomitans exhibited mutu

66 In this study, a fadA-complementing clone, F. nucleatum 12230 USF81, was constructed.

67 By contrast, F. nucleatum strains contain genomic expansions of Type

68 In contrast, F. nucleatum was able to coaggregate not only with both

69 igher levels of P. intermedia, E. corrodens, F. nucleatum, and IL-1beta than non-users.

70 is designed to determine impact of different F. nucleatum strains on neutrophil function.

71 culture in the presence/absence of different F. nucleatum strains.

72 The invasive ability may enable F. nucleatum to colonize and infect the pregnant uterus.

73 In the oral environment, F. nucleatum adheres to a large diversity of species, fa

74 Given its prevalence in EONS, F. nucleatum should be placed on the same importance sca

75 In addition to FadA, F. nucleatum ATCC 25586 and ATCC 49256 also encode two p

76 MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum,

77 dentical and were lower than those following F. nucleatum challenge alone and higher than control lev

78 eficient mice (C57BL/6 TLR2(-/-)), following F. nucleatum infection.

79 11% for Porphyromonas gingivalis to 40% for F. nucleatum.

80 may be an important virulence mechanism for F. nucleatum to infect the placenta.

81 endothelial receptor for FadA, required for F. nucleatum binding to the cells.

82 resent the first complementation studies for F. nucleatum.

83 lecular analyses demonstrated that cell-free F. nucleatum membranes are sufficient to induce cell dea

84 ious studies identified a novel adhesin from F. nucleatum, FadA, as being involved in the attachment

85 those of native FAD-I (nFAD-I) isolated from F. nucleatum ATCC 25586.

86 select subtype(s) of a given species, e.g., F. nucleatum subspecies animalis and polymorphum and S.

87 Although a trend for higher F. nucleatum and P. gingivalis concentrations in aCCP-po

88 r caspase-3 was expressed in controls and in F. nucleatum laboratory strain ATCC cocultures throughou

89 rginine-inhibitable adhesin-encoding gene in F. nucleatum that is involved in interspecies coadherenc

90 The role of TNF-alpha as an intermediary in F. nucleatum signaling was ruled out by addition of anti

91 routing into the connective tissue matrix in F. nucleatum OD-E cocultures.

92 of the outer membrane family of proteins in F. nucleatum.

93 Thus, FadA plays an important role in F. nucleatum colonization in vivo.

94 o studies to determine its potential role in F. nucleatum pathogenesis.

95 ence of a restriction-modification system in F. nucleatum.

96 the cells attached to the tooth, whereas in F. nucleatum biofilm-treated cultures, the Ki-67-express

97 t this hypothesis, we intravenously injected F. nucleatum into pregnant CF-1 mice.

98 Additionally, intravenously injected F. nucleatum localizes to mouse tumor tissues in a Fap2-

99 ent of S. cristatus to adherent and invading F. nucleatum.

100 tum was augmented compared with that of live F. nucleatum.

101 Mechanistically, F. nucleatum targeted TLR4 and MYD88 innate immune signa

102 emcomitans, A. viscosus, B. melaninogenicus, F. nucleatum, P. gingivalis, P. intermedia, S. mutans, S

103 The fadA deletion mutant F. nucleatum 12230 US1 was defective in host cell attach

104 A double-crossover fadA deletion mutant, F. nucleatum 12230-US1, was constructed by utilizing a n

105 Effects of Fusobacterium nucleatum (F. nucleatum) biofilm on epithelial cell proliferation,

106 n (Anaerobe Helsinki Negative [AHN] 9508) of F. nucleatum was placed on the top of the model.

107 The ability of F. nucleatum 12230, US1, and USF81 to colonize the mouse

108 The ability of F. nucleatum to induce apoptosis was abolished by either

109 The ability of F. nucleatum to inhibit mononuclear cell proliferation m

110 The effects of the presence or absence of F. nucleatum on anaerobe survival in both the biofilm an

111 In an equivalent culture in the absence of F. nucleatum, the numbers of black-pigmented anaerobes (

112 nsible for arginine-inhibitable adherence of F. nucleatum and provides definitive molecular evidence

113 attachment loss >2 mm, whereas the amount of F. nucleatum DNA did.

114 epidemiological evidence for association of F. nucleatum and P. aeruginosa with OSCC.

115 eractions were involved in the attachment of F. nucleatum to KB cells.

116 ractions are also involved in the binding of F. nucleatum to host cells.

117 The metabolic capabilities of F. nucleatum revealed by its genome are therefore consis

118 were aggregated in the presence of cells of F. nucleatum but not control bacteria.

119 e attachment and invasion characteristics of F. nucleatum were also tested using KB cells, an oral ep

120 present study, we showed that coinfection of F. nucleatum and T. forsythia is more potent than infect

121 were isolated from the membrane fraction of F. nucleatum ATCC 23726 and identified via mass spectros

122 Co-incubation of F. nucleatum and Escherichia coli enhanced penetration o

123 Inhibition of F. nucleatum host cell attachment and invasion with gala

124 A spontaneous mutant, lam, of F. nucleatum, isolated as defective in autoagglutination

125 A DNA library of F. nucleatum FDC 364 was constructed by partial digestio

126 Using a library of F. nucleatum mutants, we found that the Fap2 protein of

127 Possible mechanisms of F. nucleatum's role in the pathogenesis of periodontal d

128 t was significantly lower than the number of F. nucleatum-positive subjects around teeth (P < 0.05).

129 The number of F. nucleatum-positive subjects around the implant was si

130 ERT-2 epithelial cells with equal numbers of F. nucleatum and S. cristatus bacteria led to significan

131 albicans SN152 mutant library and a panel of F. nucleatum 23726 outer membrane protein mutants, we id

132 ortant for understanding the pathogenesis of F. nucleatum.

133 Although the phagocytosis pattern of F. nucleatum in the mixed infection remained similar to

134 Neutrophil phagocytosis of F. nucleatum ssp. polymorphum was significantly greater

135 Neutrophil phagocytosis of F. nucleatum ssp. polymorphum was significantly greater

136 Neutrophil phagocytosis of F. nucleatum strains and neutrophil apoptosis were analy

137 cells may reflect the invasive phenotype of F. nucleatum and contribute to the greater pathogenic po

138 ibute to the greater pathogenic potential of F. nucleatum than of S. gordonii.

139 In the presence of F. nucleatum, anaerobes persisted in high numbers (>10(7

140 m mutants, we found that the Fap2 protein of F. nucleatum directly interacted with TIGIT, leading to

141 in which tumors exploit the Fap2 protein of F. nucleatum to inhibit immune cell activity via TIGIT.

142 tokines and CCL20 suggests the broad role of F. nucleatum and human antimicrobial peptides in primary

143 uld improve our understanding of the role of F. nucleatum in periodontal infections.

144 indicate that the immunosuppressive role of F. nucleatum is largely due to the ability of this organ

145 lated bone resorption and that the strain of F. nucleatum used appeared to be the strongest inducer o

146 e findings suggest that different strains of F. nucleatum impact neutrophil function in different way

147 All strains of F. nucleatum significantly increased phagocytic capacity

148 with human and cynomolgus monkey strains of F. nucleatum.

149 l induction of hBD-2 by different strains of F. nucleatum; ATCC 25586 and ATCC 23726 induce significa

150 rphum was significantly greater than that of F. nucleatum ssp. polymorphum significantly blocked fMLP

151 rphum was significantly greater than that of F. nucleatum ssp. vincentii and ssp. nucleatum (P <0.001

152 species involved a heat-labile component on F. nucleatum and a mannan-containing heat-stable recepto

153 ached P. gingivalis but had no influences on F. nucleatum bacterial adherence.

154 valis simultaneously (at different sites) or F. nucleatum administered within 4 h prior to or 1 h fol

155 Overall, F. nucleatum and S. gordonii perturbed the gingival epit

156 he presence of any orange-complex pathogens (F. nucleatum, P. intermedia, and C. rectus), total cance

157 A may be a therapeutic target for preventing F. nucleatum colonization of the host.

158 ains, indicating that FAD-I is the principal F. nucleatum agent for hBD-2 induction in HOECs.

159 The purified F. nucleatum immunosuppressive protein (FIP) is composed

160 ore, the yeast form of C. albicans repressed F. nucleatum-induced MCP-1 and TNFalpha production in ma

161 Hemagglutination of some F. nucleatum strains is also galactose sensitive, sugges

162 Strikingly, F. nucleatum achieves virulence in the absence of large,

163 In this study, F. nucleatum was shown to activate both TLR2 and TLR4 in

164 e latter in the transwell assays, suggesting F. nucleatum may serve as an 'enabler' for other microor

165 ther of the other species in the peg system, F. nucleatum stimulated the growth of Veillonella sp. an

166 Measuring and targeting F. nucleatum and its associated pathway will yield valua

167 Thus, targeting F. nucleatum Fap2 or host epithelial Gal-GalNAc may redu

168 Recently, we demonstrated that F. nucleatum ATCC 23726 coaggregates with C. albicans SN

169 erleukin-6 (IL-6) and IL-8 demonstrated that F. nucleatum induced production of these cytokines, wher

170 tic and functional studies demonstrated that F. nucleatum promoted colorectal cancer resistance to ch

171 udies provided an initial demonstration that F. nucleatum adhered to and invaded HGEC and that this w

172 provides definitive molecular evidence that F. nucleatum adhesins play a vital role in inter-species

173 his study represents the first evidence that F. nucleatum may be transmitted hematogenously to the pl

174 We found that F. nucleatum ATCC 23726 inhibits growth and hyphal morph

175 These studies indicate that F. nucleatum aggregates PBMCs, and that this interaction

176 Data indicate that F. nucleatum impaired endothelial cell proliferation and

177 These studies indicate that F. nucleatum may facilitate the colonization of epitheli

178 The data also indicated that F. nucleatum-induced cell apoptosis requires activation

179 tic analysis of sequence data indicates that F. nucleatum, F. necrophorum, and F. varium are the spec

180 We have shown previously that F. nucleatum adheres to and invades host epithelial and

181 However, inhibitor studies show that F. nucleatum stimulation of hBD-2 mRNA requires both new

182 These data show that F. nucleatum subspecies and T. forsythia synergistically

183 Previously, it was shown that F. nucleatum induced preterm and term stillbirth in mice

184 Previous studies have shown that F. nucleatum species synergize with T. forsythia during

185 The F. nucleatum strain ATCC 25586 genome was assembled from

186 pHS17 was stably maintained in the F. nucleatum transformants, and differences in the trans

187 ine macrophage cell line, we showed that the F. nucleatum-induced inhibition of Candida hyphal morpho

188 erminal sequences of these proteins with the F. nucleatum genome revealed that the genes encoding the

189 Our data support that this F. nucleatum-mediated inhibition is mediated by human, b

190 Thus, F. nucleatum orchestrates a molecular network of the Tol

191 0018), to P. gingivalis (P = 0.0013), and to F. nucleatum (P = 0.0200) than women who delivered at te

192 In addition, polyclonal antibodies to F. nucleatum, which inhibited fusobacterial attachment t

193 Tumors from Apc(Min/+) mice exposed to F. nucleatum exhibit a proinflammatory expression signat

194 lecular level, the genetically transformable F. nucleatum strain ATCC 23726 was screened for adherenc

195 t the strong coaggregation between wild-type F. nucleatum 23726 and C. albicans SN152 in an in vitro

196 mors is inhibited in the presence of various F. nucleatum strains.

197 When F. nucleatum T18 was incubated with PHA-stimulated PBMCs

198 r the 6-month period in both groups, whereas F. nucleatum was significantly reduced in all visits in

199 This may explain why F. nucleatum is often found in mixed infections.

200 d C. albicans strains demonstrated CoAg with F. nucleatum.

201 ns grown at 37 degrees C to coaggregate with F. nucleatum.

202 ly C. dubliniensis strains coaggregated with F. nucleatum ATCC 49256 and no C. albicans strains showe

203 truction either alone or in combination with F. nucleatum.

204 were strong TLR4 agonists when combined with F. nucleatum LPS.

205 into neutrophil-like cells and cultured with F. nucleatum strains of subspecies (ssp.) nucleatum ATCC

206 incentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN).

207 Infection with F. nucleatum and P. gingivalis simultaneously (at differ

208 Primary infection with F. nucleatum plus P. gingivalis at various ratios (i.e.,

209 an gingival epithelial cells stimulated with F. nucleatum cell wall extract, indicating possible invo

210 by immunofluorescence in HGE stimulated with F. nucleatum cell wall, consistent with induction of the

211 helium, in contrast to cultures treated with F. nucleatum clinical strain AHN, in which caspase-3 was

212 the polymicrobial consortium with or without F. nucleatum exhibited significantly increased alveolar