ライフサイエンスコーパス: FAIRE

1 FAIRE (formaldehyde-assisted isolation of regulatory ele

2 FAIRE has low technical variability, which allows its us

3 FAIRE has utility as a positive selection for genomic re

4 FAIRE induction was universally decreased by Brg-1 deple

5 FAIRE performed in human cells strongly enriches DNA coi

6 The combination of DNaseI and FAIRE is more effective than either assay alone in ident

7 erse human cell types, we used DNase-seq and FAIRE-seq (Formaldehyde Assisted Isolation of Regulatory

8 detected by techniques such as DNase-seq and FAIRE-seq.

9 Regulatory elements enriched by FAIRE have high concordance with those identified by nuc

10 latory elements to extract protein-free DNA (FAIRE) and the MNase-mediated purification of mononucleo

11 de-assisted isolation of regulatory element (FAIRE)-sequencing.

12 e-Assisted Isolation of Regulatory Elements (FAIRE) procedure and use it to determine the rate of cro

13 e-assisted isolation of regulatory elements (FAIRE) quantitative PCR and chromatin immunoprecipitatio

14 e-assisted isolation of regulatory elements (FAIRE) revealed chromatin conformation changes that were

15 e-assisted isolation of regulatory elements (FAIRE) to map open chromatin during the transition from

16 e-assisted isolation of regulatory elements (FAIRE), a chemical method to enrich for nucleosome-deple

17 e-assisted isolation of regulatory elements (FAIRE).

18 e FAIRE data were compared with whole-embryo FAIRE data.

19 ong midline FAIRE peak and weak whole-embryo FAIRE peak overlap with known midline enhancers and prov

20 ith, but is distinct from, that observed for FAIRE and DNase I hypersensitive sites.

21 Supporting this notion, global FAIRE sequencing (seq) data indicated an enrichment of s

22 We looked at dynamic changes in FAIRE signals during GR activation specifically at regio

23 GR binding regions demonstrate increases in FAIRE signal in response to ligand.

24 rs7903146 showed allelic imbalance in islet FAIRE signals and that the variant alters enhancer activ

25 Midline enhancer fragments with a midline FAIRE peak tend to be near midline-expressed genes, wher

26 whereas midline enhancers without a midline FAIRE peak were often distant from midline-expressed gen

27 regions of the genome with a strong midline FAIRE peak and weak whole-embryo FAIRE peak overlap with

28 midline cells was performed and the midline FAIRE data were compared with whole-embryo FAIRE data.

29 Comparison of FAIRE-seq data from islets to that from five non-islet c

30 Evidence for cell-type-specific patterns of FAIRE enrichment is also presented.

31 map "open chromatin." Over 870,000 DNaseI or FAIRE sites, which correspond tightly to nucleosome-depl

32 Our FAIRE results were compared with DNA-methylation analysi

33 In addition, our FAIRE-seq analysis allowed us to identify regulatory ele

34 To perform FAIRE, chromatin is crosslinked with formaldehyde in viv

35 NCOR1 and H3K9me3 ChIP-Seq, FAIRE-seq and DNA CpG methylation interactions were rela

36 ulatory regions in DNA (DNase-seq, ChIP-seq, FAIRE-seq, ATAC-seq).

37 hort-read sequencing technologies (ChIP-seq, FAIRE-seq, MNase-Seq, ...) and offers innovative approac

38 ble chromatin landscape including DNase-seq, FAIRE-seq and ATAC-seq.

39 nomic assays, including ChIP-seq, DNase-seq, FAIRE-seq and others.

40 data from a variety of ChIP-seq, DNase-seq, FAIRE-seq, and ATAC-seq experiments, we show that our we

41 s) combined with next-generation sequencing (FAIRE-seq) to identify specific changes in chromatin acc

42 ents followed by next-generation sequencing (FAIRE-seq) to map regions of open chromatin in three pri

43 nts coupled with high-throughput sequencing (FAIRE-seq).

44 ority GR-responsive regions shared a similar FAIRE signal in the basal chromatin state, suggesting a

45 In this study, FAIRE-seq on FACS-purified midline cells was performed a

46 Using 548 ENCODE ChIP-seq and six targeted FAIRE-seq samples, we show that BaalChIP effectively cor

47 me-depleted DNA from human chromatin, termed FAIRE (Formaldehyde-Assisted Isolation of Regulatory Ele

48 ion of cis-regulatory elements and show that FAIRE-seq can guide the identification of regulatory var

49 We modified the FAIRE procedure to allow us to examine chromatin accessi

50 at drive midline expression in vivo with the FAIRE data.