ライフサイエンスコーパス: FAST

1 FAST and CT contribute to triage, guide surgical managem

2 FAST and CT scans undertaken by attending radiologists c

3 FAST enables complete visualization of the brain at a re

4 FAST examinations were performed in 1540 patients (1227

5 FAST examinations were performed on patients with precor

6 FAST mice were more sensitive to the stimulant effects o

7 FAST mice were more stimulated than SLOW mice by all dru

8 FAST renders unbiased quantitative group comparisons of

9 FAST was inadequate in 3.7%, 28.9% demonstrated cardiac

10 FAST(-/-) mice develop airway inflammation that is disti

11 FAST-1 has been shown to associate with Smad2 and Smad4,

12 FAST-1 recognition of the ARE is essential both for ARF

13 FAST-2 also interacts directly with Smad2, a cytoplasmic

14 actor fork head activin signal transducer 1 (FAST-1) was identified as a mediator of activin signalin

15 teractive Thresholding Algorithm [SITA] 24-2 FAST), in a single sitting, using standardized clinical

16 Five trauma surgeons performed 546 FAST examinations during the study period.

17 NIRF reporter of MMP activity (MMPSense-645-FAST), both probes produced statistically significant in

18 A FAST examination including parasternal/subxiphoid cardia

19 ons with the translational silencer TIA-1: a FAST mutant lacking its TIA-1-binding domain does not in

20 Conversely, expression of a FAST-1-repressor fusion (FAST-En(R)) in prospective ecto

21 ectopic expression of mutationally activated FAST-1, ectopic expression of dominant inhibitory FAST-1

22 n of constitutively transcriptionally active FAST-1 fusion protein (FAST-VP16(A)) in prospective ecto

23 All FAST interpretations were validated by at least one of f

24 Analysis of the sequences of FAST-1 and FAST-2 reveals substantial protein sequence divergence c

25 urated hydrocarbon chains (e.g. DiIC(12) and FAST DiI) enter the endocytic recycling compartment effi

26 rdial Infarction (FAST-MI) 2005 (n=3670) and FAST-MI 2010 (n=4169).

27 iewed the complete AP (consisting of MIP and FAST images and optionally their nonsubtracted source im

28 dihexadecyloxacarbocyanine perchlorate) and FAST DiO (3, 3'-dilinoleyloxacarbocyanine perchlorate) b

29 etramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarb

30 method outperforms prior tools (QuSHAPE and FAST) significantly in terms of accuracy compared with g

31 The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth

32 ty of Smad4 to form a complex with Smad2 and FAST on the Mix.2 promoter.

33 potential survivors after traumatic arrest, FAST represents an effective method of separating those

34 Because FAST is released from mitochondria in cells undergoing F

35 Because FAST(-/-) neutrophils exhibit normal chemotaxis and surv

36 have mapped the site of interaction between FAST-1 and Smad2/Smad4 to a novel carboxy-terminal domai

37 Both FAST and TIA1 are also found in the nucleus, where TIA1

38 Both FAST and TIA1 target a U-rich intronic sequence (IAS1) a

39 Both FAST-1 and Smads can bind directly to the ARE; we have i

40 We find that ARF binds to ARE through both FAST-1 and Smad binding sites.

41 lated Akt phosphorylation were unaffected by FAST versus BFAST in both lean and obese cohorts (all P

42 We find that this gene, which we call FAST-2, is able to mediate transcriptional activation by

43 In conclusion, FAST allows for high-throughput data processing to match

44 In obese individuals, no differential (FAST versus BFAST) expression was observed in genes invo

45 nhancer in the mouse nodal gene, and directs FAST site-dependent expression in the primitive streak d

46 nterference-mediated knockdown of endogenous FAST results in apoptosis, whereas overexpressed recombi

47 the maternally encoded transcription factor FAST-1 in the establishment of the mesodermal transcript

48 and TGF-beta through Smads and the WH factor FAST-2.

49 , and a novel forkhead transcription factor, FAST-1, and binds to an enhancer (activin-responsive ele

50 temporally restricted transcription factor, FAST-1.

51 tion by a winged-helix transcription factor, FAST-2, on an activin-responsive element (ARE) in the Xe

52 before and after 6 weeks of morning fasting (FAST; 0 kcal until 12.00 h) or daily breakfast consumpti

53 4 for a cutoff of > or =6 points vs 0.51 for FAST stage 7c).

54 records to determine diagnostic accuracy for FAST and CT and their influence on casualty management.

55 ted by the same domain which is required for FAST-2 to interact with Smad2.

56 se element, a high-affinity binding site for FAST-1.

57 njection of a blocking antibody specific for FAST-1 prevents induction of mesodermal response genes b

58 njection of a blocking antibody specific for FAST-1.

59 the winged-helix transcription factor FoxH1 (FAST), a critical component of a positive feedback loop

60 nd TATA box as well as potential Smad, FoxH1/FAST, T-box, COUP-TF, C/EBP, GATA, HNF3 binding sites an

61 of 17 individual QIs could be assessed from FAST-MI 2010.

62 term storage the modest changes in furosine, FAST index and browning in ginger cake formulated with d

63 Furthermore, FAST is highly scalable to non-human primate brains and

64 ly, expression of a FAST-1-repressor fusion (FAST-En(R)) in prospective ectoderm blocks induction of

65 Mice selectively bred for high (FAST) or low (SLOW) locomotor stimulant response to etha

66 ly characterized to associate with the human FAST-1 protein.

67 Consistent with this hypothesis, FAST promotes the expression of cotransfected reporter p

68 embryonic tissue explant assays, identifying FAST as an essential mediator of Xnr autoregulation and/

69 In FAST-MI 2010, 12 individual and 2 composite QIs could be

70 ity to allopregnanolone was also measured in FAST and SLOW mice, which were selectively bred for diff

71 Four QIs were not recorded in FAST-MI 2010 and 4 in 2005, either because of treatment

72 eutrophil recruitment is markedly reduced in FAST(-/-) mice compared with wild type controls.

73 n or Non-ST-Elevation Myocardial Infarction (FAST-MI) 2005 (n=3670) and FAST-MI 2010 (n=4169).

74 n or Non-ST Elevation Myocardial Infarction (FAST-MI) 2005 according to use and type of reperfusion t

75 1, ectopic expression of dominant inhibitory FAST-1, and injection of a blocking antibody specific fo

76 For detection of intra-abdominal injury, FAST sensitivity (Sn) was 0.56, specificity (Sp) 0.98, p

77 In conclusion, our results introduce FAST as a proinflammatory factor that modulates the func

78 he principal DNA-binding component of ARF is FAST-1, a transcription factor with a novel winged-helix

79 s are available at www.cs.cornell.edu/~keich/FAST.

80 describe a high-throughput NMR methodology (FAST-NMR) to annotate the biological function of novel p

81 eonine phosphoprotein (FAST)-deficient mice (FAST(-/-)) to study the in vivo role of FAST in immune s

82 speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which ac

83 l injury; 26 (17%) of whom also had negative FAST studies.

84 Our data reveal that nuclear FAST can regulate the splicing of FGFR2 transcripts.

85 In a gel shift assay, the association of FAST-2 with Smad4 was mutually exclusive from the associ

86 The combination of FAST enrichment and ADM imaging has the performance requ

87 ognition of the ARE, 2) the contributions of FAST-1 and Smad binding to ARF binding in vitro and to A

88 /Smad4 to a novel carboxy-terminal domain of FAST-1, and find that overexpression of this domain spec

89 The antiapoptotic effects of FAST are regulated by interactions with the translationa

90 Because the antiapoptotic effects of FAST require ongoing protein synthesis, we hypothesized

91 TIA-1 inhibits the antiapoptotic effects of FAST.

92 ng is separable from the survival effects of FAST.

93 dermal genes by activin, while expression of FAST-En(R) in intact embryos prevents general/dorsal mes

94 Furthermore, the interaction of FAST-2 with BF-1 is mediated by the same domain which is

95 ice (FAST(-/-)) to study the in vivo role of FAST in immune system function.

96 the ARE; we have investigated 1) the role of FAST-1 and Smad DNA binding sites in ARF recognition of

97 Analysis of the sequences of FAST-1 and FAST-2 reveals substantial protein sequence d

98 surgeons who are newly trained in the use of FAST can achieve an overall accuracy rate of at least 90

99 ED following blunt torso trauma, the use of FAST compared with standard care only did not improve cl

100 e findings do not support the routine use of FAST in this setting.

101 The authors also report on the utility of FAST in special patient populations, such as pediatric a

102 bdominal visceral injury limits the value of FAST as a screening diagnostic modality for patients who

103 abeled IgG and bovine serum albumin (BSA) on FAST, Unisart, and Oncyte-Avid slides and compared the s

104 x and that this association was dependent on FAST-2.

105 Cardiac motion on FAST was 100% sensitive and 73.7% specific for the ident

106 raphic examination of the trauma patient, or FAST, is replacing central venous pressure measurements

107 the cytosolic tail of reptilian reovirus p14 FAST protein functions as a novel tribasic Golgi export

108 on factor SARA and the nuclear Smad2 partner FAST-1 for binding to a hydrophobic corridor on the MH2

109 s-activated serine/threonine phosphoprotein (FAST) is a survival protein that is tethered to the oute

110 s-activated serine/threonine phosphoprotein (FAST) is tethered to the outer mitochondrial membrane, w

111 s-activated serine threonine phosphoprotein (FAST)-deficient mice (FAST(-/-)) to study the in vivo ro

112 NA binding partner, the winged helix protein FAST-1.

113 nscriptionally active FAST-1 fusion protein (FAST-VP16(A)) in prospective ectoderm can directly induc

114 The qualitative FAST results and FAST-derived MIC (MICFAST) correspond c

115 In contrast, r-FAST and r-SLOW mice differed in sensitivity to only a f

116 lar in sensitivity to ethanol stimulation (r-FAST and r-SLOW) and provided a unique model for testing

117 More compellingly, recombinant FAST increases the expression of endogenous cIAP-1 and X

118 apoptosis, whereas overexpressed recombinant FAST inhibits Fas- and UV-induced apoptosis, indicating

119 have also demonstrated the ability of SHAPE/FAST to detect the binding of a small molecule inhibitor

120 t Smad4 stabilizes a ligand-stimulated Smad2-FAST-1 complex as an active DNA-binding factor.

121 n, Smad4 promotes binding of the Smad2/Smad4/FAST-1 complex to DNA; through its carboxy-terminal doma

122 ortex and the Functional Assessment Staging (FAST) measure of the clinical severity of AD at the time

123 of 7c on the Functional Assessment Staging (FAST) scale.

124 In cells subjected to environmental stress, FAST moves to stress granules, where it interacts with T

125 rived images (first postcontrast subtracted [FAST] and maximum-intensity projection [MIP] images), wa

126 We call this technique FAST CARS (femtosecond adaptive spectroscopic techniques

127 ) and fiber-optic array scanning technology (FAST) to identify and image cells expressing HIV Gag.

128 uses fiber-optic array scanning technology (FAST), which applies laser-printing techniques to the ra

129 flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-

130 eport that Smad4 is present in ARF, and that FAST-1, Smad4 and Smad2 co-immunoprecipitate in a ligand

131 complementary approaches to demonstrate that FAST-1 is a central regulator of mesoderm induction by e

132 d inhibit axis formation, demonstrating that FAST-1 is a necessary component of the first steps in th

133 The finding that FAST is concentrated at nuclear speckles also supports t

134 oing protein synthesis, we hypothesized that FAST might function by preventing TIA-1-mediated silenci

135 s- and UV-induced apoptosis, indicating that FAST is a survival protein.

136 as- or UV-induced apoptosis, we propose that FAST serves as a sensor of mitochondrial stress that mod

137 prisingly, knockdown experiments reveal that FAST and TIA1 act independently of one another to promot

138 screens and biochemical analysis reveal that FAST binds to several alternative and constitutive splic

139 Mutational analysis reveals that FAST-mediated alternative splicing is separable from the

140 We show that FAST, like TIA1, promotes the inclusion of exon IIIb of

141 itutive splicing regulators, suggesting that FAST might participate in this process.

142 This suggests that FAST-2 represents a new WH gene related to FAST-1, which

143 The FAST cassette is similar in an ascidian nodal-related ge

144 The FAST is a rapid test that sequentially surveys the peric

145 The FAST package includes C++ implementations of various alg

146 The FAST-1/Smad2/Smad4 complex binds and activates a 50 bp a

147 Although the FAST exam is not recommended as the sole screening tool

148 In a yeast two-hybrid assay, the FAST-1 carboxy terminus interacts with Smad2 but not Sma

149 patients experiencing HAE attacks during the FAST-2 open-label extension (OLE) phase.

150 of the liquid droplet was comparable for the FAST and Unisart slides but different, i.e., slower, for

151 To address this, we present here the FAST package, an open-source collection of programs and

152 To determine if the FAST examination during initial evaluation of injured ch

153 Median hospital charges were $46415 in the FAST group and $47759 in the standard care-only group (d

154 minal CT scans was 241 of 460 (52.4%) in the FAST group and 254 of 465 (54.6%) in the standard care-o

155 mean ED length of stay was 6.03 hours in the FAST group and 6.07 hours in the standard care-only grou

156 bdominal injury occurred in a patient in the FAST group and none in the control group (difference, 0.

157 valuable patients with cSSSI enrolled in the FAST II trial from 11 sites in the United States (56 iso

158 art review will discuss the evolution of the FAST examination to its current state in 2017 and evalua

159 Deletion mutants of the FAST-1 carboxy terminus that still participate in ligand

160 We have also examined the structure of the FAST-2 gene and find that it overlaps with a kinesin mot

161 s was nonhomogenous and doughnut-like on the FAST and Unisart nitrocellulose slides, whereas a better

162 ive icatibant-treated attacks throughout the FAST-2 OLE phase.

163 L1/ETO, contains a region of homology to the FAST proteins, which cooperate with Smads to regulate tr

164 Today the FAST examination has evolved into a more comprehensive s

165 hin 4 h of ICH onset; a phase III trial (the FAST trial) is now in progress.

166 The clinical conditions in which the FAST is most accurate in the assessment of injured patie

167 ned to a standard trauma evaluation with the FAST examination by the treating ED physician or a stand

168 nd that Smad3 was able to associate with the FAST-2.ARE complex and that this association was depende

169 was also able to directly associate with the FAST-2.ARE complex through binding with FAST-2.

170 Intron 1 contains three FAST binding sites on which FAST/Smad transcriptional co

171 alone was able to stimulate the ARE through FAST-2, but inhibited the ARE transactivation mediated b

172 Thus, FAST provides new opportunities for global approaches th

173 However, unlike TIA1, FAST does not bind to the IAS1 sequence.

174 Prior to FAST, invasive procedures such as diagnostic peritoneal

175 t FAST-2 represents a new WH gene related to FAST-1, which functions to mediate TGF-beta signals in m

176 gram entitled Fast Analysis of SHAPE traces (FAST), which significantly reduces processing time.

177 e p15 fusion-associated small transmembrane (FAST) protein is a nonstructural viral protein that indu

178 virus fusion-associated small transmembrane (FAST) proteins comprise a unique family of viral membran

179 cused assessment with sonography for trauma (FAST) examination in children is unknown.

180 cused Assessment with Sonography for Trauma (FAST) has modest sensitivity for hemoperitoneum and IAI

181 used Assessment Using Sonography for Trauma (FAST) to discriminate between survivors and nonsurvivors

182 of focused abdominal sonography for trauma (FAST).

183 ocused assessment with sonography in trauma (FAST) has been extensively utilized and studied in blunt

184 The For Angioedema Subcutaneous Treatment (FAST)-2, a phase III, double-blind, randomized, multicen

185 of an international phase 2 clinical trial (FAST II) evaluating telavancin for the treatment of comp

186 ly gathered data for all patients undergoing FAST for blunt trauma during a 30-month period.

187 inclusion criteria, of whom 85.0% underwent FAST and 86.1% abdominal CT; 159 (34.0%) had abdominal i

188 ts arrived in traumatic arrest and underwent FAST.

189 Trauma surgeons are routinely using FAST to evaluate patients with blunt trauma for hemoperi

190 is then delivered to late endosomes, whereas FAST DiI, with two cis double bonds in each tail, and Di

191 ARE in a DNase I protection assay, in which FAST-2 binds the ARE around a motif (TGTGTATT) previousl

192 1 contains three FAST binding sites on which FAST/Smad transcriptional complexes can assemble; these

193 With FAST cytometry, laser-printing optics are used to excite

194 We demonstrate that BF-1 associates with FAST-2.

195 ory activity by competitive association with FAST-2.

196 the FAST-2.ARE complex through binding with FAST-2.

197 o the nucleus where they form a complex with FAST-1 that requires these three components to activate

198 al responses to TGF-beta by interacting with FAST-2 or with other DNA binding proteins which function

199 Interference with FAST function abolishes intron 1 activity, and transcrip

200 the mouse which shares many properties with FAST-1.

201 Y-FAST distinguishes itself from other tagging systems bec

202 Y-FAST is as bright as common fluorescent proteins, exhibi

203 Y-FAST was engineered from the 14-kDa photoactive yellow p

204 ce-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large as t

205 the Zometa-Femara Adjuvant Synergy Trial (Z-FAST) indicate that upfront zoledronic acid therapy prev