ライフサイエンスコーパス: FCS

1 FCS allows assay miniaturization without compromising se

2 FCS analysis detailed the diffusion characteristics and

3 FCS analysis of a green fluorescent protein (GFP)-tagged

4 FCS analysis of CA200645-occupied A3ARs revealed 2 speci

5 FCS and PCH analysis of fluorescence-tagged 5-HT(2C) rec

6 FCS brightness analysis showed that heterodimerization o

7 FCS measurements from solution mixtures of dye-labeled p

8 FCS measurements in small-membrane microdomains (approxi

9 FCS measurements indicate that pH-dependent formation of

10 FCS measurements revealed hindered diffusion of lipids i

11 FCS measurements show that PIP(2) diffuses rapidly (D ~

12 FCS measures the spatial and temporal correlation of ind

13 FCS provided plasma membrane diffusion coefficients on t

14 FCS was used to record fluctuations in fluorescence inte

15 FCS with PCH revealed molecular brightness values for na

16 etal calf serum (FCS) were incubated in 0.1% FCS with 1, 10, or 40 mM lactate or PBS for 5 and 30 min

17 concentrations with proper serum levels (10% FCS) and growth factor combinations (EGF, NGF) yielded t

18 med human LECs were cultured in standard 10% FCS-DMEM containing various concentrations of sugar.

19 ntation induced by Spider media and YPD, 10% FCS, and biofilm formation and macrophage killing were u

20 with denaturant concentration obtained by 2f-FCS and DLS.

21 us fluorescence correlation spectroscopy (2f-FCS) to characterize the denaturant dependence of the un

22 Additionally, FCS was used to characterize the fraction of fluorescent

23 le cameras with frame rates of 1000 Hz allow FCS measurements of systems with diffusion coefficients

24 Analyzing FCS data with suitable kinetic model we obtain transient

25 CFM and FCS complement each other ideally and can be realized us

26 ical and computational framework for CFM and FCS experiments using 4Pi microscopy, which features an

27 So far, the spatial resolution of CFM and FCS was restricted by the resolution of the light micros

28 t groups after exposure to dexamethasone and FCS, and these were associated with biological pathways

29 ASMCs were exposed to dexamethasone and FCS.

30 Manually obtained measurements of FCP and FCS were also compared.

31 alyze the SMT data and to guide the FRAP and FCS analysis, we show how all three approaches yield sim

32 easure the diffusion coefficient by FRAP and FCS in the exact same images.

33 When this effect is minimized, FRAP and FCS measurements nearly agree, although cross-validation

34 n concentrations typically used for FRAP and FCS, nor is it likely due to spatial heterogeneity of th

35 s also shown how the combination of SICS and FCS can be used to determine the fraction of fluorescent

36 g droplets for noise reduction by averaging, FCS can monitor accurately the droplets flow even if the

37 parameters on the capability of camera-based FCS to determine membrane heterogeneity via the FCS diff

38 ficient experimental design for camera-based FCS to extract information on mobility, concentration, a

39 d concentration measurements in camera-based FCS.

40 During the working-memory experiment, both FCS-rCBF coupling and rCBF/FCS ratio were modulated by t

41 al Gaussian geometry ubiquitously assumed by FCS theory, or when properties of multiple fluorescent s

42 peptides reach the cytosol, as determined by FCS, correlates directly with their efficacy in cell-bas

43 ses without denaturing agent as evidenced by FCS and gel-based multimer analysis.

44 binding to IL8 and IL6 promoters induced by FCS+TGF-beta.

45 at the equilibrium distributions measured by FCS and PCH deviated from those obtained from the meltin

46 RAP is 15 times longer than that obtained by FCS.

47 The reaction being probed by FCS and PCH is suggested to be a rapid equilibrium betwe

48 By analyzing live-cell FCS measurements, we show that our new model can satisfa

49 Combining FCS, DLS, and TEM results, we find three distinct period

50 By combining FCS and video-microscopy (with PTV and PIV processing ap

51 Overall, combining FCS, PIV, and PTV analysis of redox-MHD is a powerful co

52 Finally, we apply spinning disk confocal FCS to microspheres diffusing in Type I collagen, which

53 sing particles or molecules; we demonstrated FCS for sizing synaptic vesicles confined in aqueous dro

54 or manually derived FCP and manually derived FCS thickness was 0.94 (P < 0.001), with a smaller mean

55 esting multiple competing models to describe FCS data based on temporal autocorrelation functions.

56 ctions introduced by the DNA binding domain, FCS generates independent estimates for the diffusion co

57 d test their predictions against our earlier FCS measurements of the vesicle dynamics.

58 Here, we apply this procedure to evaluate FCS data from fluorescent proteins assayed in vitro and

59 en aggregation and toxicity are exacerbated, FCS-based burst analysis and purified single molecule FC

60 ons support the analysis of the experimental FCS data.

61 Hence, extending FCS for periodically passing objects converts it into a

62 mented resonant line-scan STED with filtered FCS, which has the additional benefit of autocalibrating

63 nd fractions, or even fail altogether to fit FCS binding data.

64 ive internal reflection geometry adapted for FCS.

65 he necessity of a photobleach correction for FCS measurements of GFP-tagged molecules that are bound

66 a new and potentially interesting field for FCS studies could be the study of nonequilibrium steady

67 of competing, non-nested physical models for FCS data, appropriately penalizing model complexity acco

68 d that the mode symmetry selection rules for FCS are the same as for Raman scattering and that both F

69 ed these nanoparticles using ITC, DLS, FRET, FCS, TIRF, and TEM.

70 FRET-FCS and sm-FRET measurements are comparable and show tha

71 FRET-FCS could readily detect a FRET-active oligonucleotide p

72 When applied to the analysis of Abeta, FRET-FCS detected oligomers consisting of less than 10 Abeta

73 distances is relatively narrow for both FRET-FCS and sm-FRET, suggesting that the two packaged DNA en

74 This ability of FRET-FCS could be an indispensable tool for studying biologic

75 Fluorescence Correlation Spectroscopy (FRET-FCS) has a unique ability to detect small subpopulations

76 From FCS autocorrelation functions, we obtain the number of f

77 ical mechanistic insight is often drawn from FCS experiments by fitting the resulting time-intensity

78 ion of model parameter values estimated from FCS data.

79 roach to directly measure binding rates from FCS data.

80 Further FCS measurements involving urea and NaCl show that the u

81 On the other hand, FCS studies show an unexpected increase the mobility of

82 ver, based on the principles described here, FCS can be straightforwardly applied for a variety of sy

83 ing state, functional brain hubs with higher FCS were identified, primarily in the default-mode, insu

84 However, FCS data analysis and interpretation using fluorescent p

85 addition to its practical utility, however, FCS provides a window on mesoscopic systems in which flu

86 With the advent of camera-based imaging FCS, which measures the diffusion coefficient in each pi

87 biased model-based interpretation of imaging FCS data, with broad applicability to resolving the hete

88 ional analysis and interpretation of imaging FCS datasets.

89 As proof of concept, imaging-FCS was used to measure surface diffusion rates, interfa

90 combination of these two techniques, imaging-FCS, for measurement of fast interfacial transport at a

91 .5 years, all sensation thresholds improved (FCS: P = 0.002; DDV: P = 0.002; MTV: P = 0.002), but cha

92 ds, specified within the multiple imputation FCS method, for selected predictors for each operation u

93 In FCS experiments, we found that the catch bond character

94 y assess the latent information contained in FCS datasets.

95 The mean error in FCS retinal thickness measurement increased as the densi

96 By using simple modifications in FCS setup, we describe how one can extract the reaction

97 te that photobleaching of bound molecules in FCS is mainly responsible.

98 e highly correlated noise that is present in FCS data sets and additionally penalizes model complexit

99 JQ1/SGCBD01 and I-BET762 inhibited FCS+TGF-beta-induced ASM cell proliferation and IL-6 and

100 -fluorescence correlation spectroscopy (ITIR-FCS) showed that monomeric hIAPP induced the formation o

101 eveloped Bayesian analysis procedure to ITIR-FCS data to resolve hIAPP-induced microdomain spatial or

102 igher than possible with diffraction-limited FCS.

103 les passively moving in a homogeneous media, FCS analysis yields analytical functions that can be fit

104 tion experiment on the proposed microfluidic-FCS platform is accomplished using only picograms of pro

105 ly implemented on a fluorescence microscope, FCS samples femtoliter volumes and so is especially usef

106 burst analysis and purified single molecule FCS detected a populational shift toward an increase in

107 ed DNA plasmid, we show that single-molecule FCS can distinguish between bursts from species that dif

108 Single-molecule FCS is a useful procedure for measuring dissociation equ

109 We also introduce "single-molecule FCS", which obtains diffusion time estimates for each bu

110 n fluorescence correlation spectroscopy (MPE-FCS).

111 We apply this new FCS method to evaluate the efficacy of a potential antic

112 culture supernatant fluid in the absence of FCS.

113 s of objects, the collection and analysis of FCS data have to be reconceptualized.

114 Current analysis of FCS data is largely based on the assumption that the lab

115 Global analysis of FCS data provides association (k(+)) and dissociation (k

116 Here, we extended the applicability of FCS for the detection and analysis of periodically passi

117 foundation for the automated application of FCS to the analysis of biological and other complex samp

118 Finally, task-induced changes of FCS and rCBF in the lateral-parietal lobe positively cor

119 However, the inherent drawback of FCS is that species with similar molecular weight could

120 gnal is retained and the functional forms of FCS fitting equations remain valid.

121 , we report the successful implementation of FCS, FCCS, and PCH in live yeast cells using fluorescent

122 Interpretation of FCS data relies critically on objective multiple hypothe

123 The unbiased interpretation of FCS data relies on the evaluation of multiple competing

124 complexity to prevent over interpretation of FCS data.

125 In order to better understand the limits of FCS, this study systematically explores the relationship

126 In addition, although measurement of FCS remains preferable for assessment of central retinal

127 oscopy (SPIM-FCS), a multiplexed modality of FCS, which generates maps of molecular dynamics, concent

128 In addition to eliminating overfitting of FCS data, the procedure dictates when the interpretation

129 imulations, which predict the performance of FCS under a variety of experimental circumstances.

130 These results demonstrate the potential of FCS as a sensitive probe of dynamic and functionally imp

131 To utilize the power of FCS for systems with nonstochastic displacements of obje

132 ly regulated after growth in the presence of FCS.

133 system was downregulated by the presence of FCS.

134 ue to typically low signal-to-noise ratio of FCS data and correlated noise in autocorrelated data set

135 Moreover, because of the high sensitivity of FCS, this method allows examination of the effect of AMP

136 Here, we extend the use of FCS to low concentrations of coding RNAs in single livin

137 sing rate parameter values determined in our FCS experiments, could reconstruct both the observed mem

138 We find that our FCS estimates are quantitatively consistent with our flu

139 h fluorescence correlation spectroscopy (PET-FCS).

140 S and compare PIE-RICS with single-point PIE-FCS measurements.

141 ethod to simulate single- and multiple-point FCS, photon-counting histogram analysis, raster image co

142 Measurement using conventional single-point FCS at every individual pixel results in continuous long

143 otational diffusion as assessed by polarized FCS and previous multi-frequency (1)H NMR relaxometry ex

144 We present FCS and STED experiments showing that dense membrane bou

145 As a remedy, we introduce "purified FCS", which uses single molecule burst analysis to selec

146 We demonstrate the use of purified FCS in experiments with DNA sliding clamps.

147 experiment, both FCS-rCBF coupling and rCBF/FCS ratio were modulated by task load in the ECN and/or

148 er rCBF per unit connectivity strength (rCBF/FCS ratio); whereas, this index was lower in posterior v

149 In particular, using real FCS data, we describe how the effects of cell crowding a

150 cal processes, as well as spatially resolved FCS from image correlation spectroscopy, providing an im

151 ffected by the presence of fetal calf serum (FCS) in the growth medium.

152 uman TM cells grown in 10% fetal calf serum (FCS) were incubated in 0.1% FCS with 1, 10, or 40 mM lac

153 essential medium, MEM) and fetal calf serum (FCS) were probed by XANES and EXAFS.

154 We thus performed simultaneous FCS and FRAP measurements on supported lipid bilayers an

155 n of ice and a freeze-concentrated solution (FCS) are poorly understood in spite of their importance

156 irst used a fully conditional specification (FCS) multiple imputation method to establish complete da

157 oscopy (AFM) based force clamp spectroscopy (FCS) and dynamic force spectroscopy (DFS).

158 4) using femtosecond coherence spectroscopy (FCS) in combination with polarized resonance Raman spect

159 rrelation or cross-correlation spectroscopy (FCS or FCCS), a single molecule technique, has the abili

160 ) and fluorescence correlation spectroscopy (FCS) also reveal divalent metal ion (Me(2+))-induced clu

161 mbine fluorescence correlation spectroscopy (FCS) and a microfluidic shear cell to monitor real-time

162 used fluorescence correlation spectroscopy (FCS) and alanine-scanning mutagenesis to characterize th

163 based fluorescence correlation spectroscopy (FCS) and can be isolated on a solid substrate for single

164 used fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRA

165 Fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRA

166 Fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) are techniques

167 RAP), fluorescence correlation spectroscopy (FCS) and single-molecule tracking (SMT).

168 Here fluorescence correlation spectroscopy (FCS) and transmission electron microscopy (TEM) are comp

169 used Fluorescence Correlation Spectroscopy (FCS) and two photon excitation to determine the translat

170 using fluorescence correlation spectroscopy (FCS) and two-photon microscopy to measure flow speeds an

171 ) and fluorescence correlation spectroscopy (FCS) are the two most direct methods to measure the diff

172 e and fluorescence correlation spectroscopy (FCS) assays showed that approximately 20% of the substra

173 from fluorescence correlation spectroscopy (FCS) autocorrelation data.

174 r the fluorescence correlation spectroscopy (FCS) autocorrelation function for the two models and tes

175 iple, fluorescence correlation spectroscopy (FCS) based methods can provide such data, but live-cell

176 , how fluorescence correlation spectroscopy (FCS) can be used to study binding kinetics of ligands wi

177 Fluorescence correlation spectroscopy (FCS) can resolve the intrinsic fast-blinking kinetics (F

178 that fluorescence correlation spectroscopy (FCS) combined with real-time tracking of the center of m

179 R and fluorescence correlation spectroscopy (FCS) experiments combined with small-angle X-ray scatter

180 using fluorescence correlation spectroscopy (FCS) experiments, which reveal a systematic reduction of

181 with fluorescence correlation spectroscopy (FCS) for simultaneous analysis of labeled and unlabeled

182 years fluorescence correlation spectroscopy (FCS) has become a routine method for determining diffusi

183 Fluorescence correlation spectroscopy (FCS) has permitted the characterization of high concentr

184 using fluorescence correlation spectroscopy (FCS) in a microfluidic system.

185 ed by fluorescence correlation spectroscopy (FCS) in different alcohol solutions.

186 using fluorescence correlation spectroscopy (FCS) in vitro.

187 Fluorescence correlation spectroscopy (FCS) is a noninvasive technique that probes the diffusio

188 Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility

189 Fluorescence correlation spectroscopy (FCS) is a powerful approach to characterizing the bindin

190 Fluorescence correlation spectroscopy (FCS) is a powerful technique to investigate molecular dy

191 Fluorescence correlation spectroscopy (FCS) is a powerful tool to infer the physical process of

192 Fluorescence correlation spectroscopy (FCS) is a sensitive technique commonly applied for study

193 ) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcrip

194 Fluorescence correlation spectroscopy (FCS) methods are powerful tools for unveiling the dynami

195 ques (fluorescence correlation spectroscopy (FCS) or raster-scan image correlation spectroscopy) or p

196 aging fluorescence correlation spectroscopy (FCS) performed using array detectors has been successful

197 ) and fluorescence correlation spectroscopy (FCS) permit measurement of molecular mobility and associ

198 e via fluorescence correlation spectroscopy (FCS) provide a sensitive means of probing the underlying

199 nted, fluorescence correlation spectroscopy (FCS) reveals numerous static and dynamic properties of m

200 ition fluorescence correlation spectroscopy (FCS) showed that cytosolic Bax diffuses much slower than

201 hed a fluorescence correlation spectroscopy (FCS) system to measure the percentage of doubly labeled

202 ion of Fluorescent Correlation Spectroscopy (FCS) techniques.

203 used fluorescence correlation spectroscopy (FCS) to accurately and precisely determine the relative

204 ue of fluorescence correlation spectroscopy (FCS) to investigate the diffusional characteristics of f

205 ) and fluorescence correlation spectroscopy (FCS) to monitor the movement of an individual donor-labe

206 e use fluorescence correlation spectroscopy (FCS) to probe the mechanism of peptide modulation of alp

207 e use fluorescence correlation spectroscopy (FCS) to quantitatively and accurately measure flow speed

208 e use fluorescence correlation spectroscopy (FCS) to show that while protein A, a multi-domain and pr

209 with fluorescence correlation spectroscopy (FCS) to study the transition between acid-denatured stat

210 d for fluorescence correlation spectroscopy (FCS) under pulsed stimulated emission depletion (STED).

211 on of fluorescence correlation spectroscopy (FCS) using a spinning disk confocal microscope.

212 ; and fluorescence correlation spectroscopy (FCS) was further used to investigate the diffusion of BS

213 Fluorescence correlation spectroscopy (FCS) was used to characterize the diffusion of fluoresce

214 Fluorescence correlation spectroscopy (FCS) was used to determine the affinity of parental (hom

215 Fluorescence correlation spectroscopy (FCS) was used to investigate the hydrodynamic and photop

216 y and fluorescence correlation spectroscopy (FCS) we found out that, upon contact with the membrane,

217 using fluorescence correlation spectroscopy (FCS) with photon counting histogram (PCH) analysis, a se

218 using fluorescence correlation spectroscopy (FCS) with photon counting histogram (PCH).

219 used fluorescence correlation spectroscopy (FCS), a method that offers single-molecule resolution.

220 ICS), fluorescence correlation spectroscopy (FCS), and atomic force microscopy (AFM).

221 FRET, fluorescence correlation spectroscopy (FCS), and biolayer interferometry to develop a systemati

222 Fluorescence correlation spectroscopy (FCS), dynamic light scattering (DLS), and transmission e

223 Fluorescence correlation spectroscopy (FCS), fluorescence cross-correlation spectroscopy (FCCS)

224 Using fluorescence correlation spectroscopy (FCS), intermolecular fluorescence resonance energy trans

225 ) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an o

226 Using fluorescence correlation spectroscopy (FCS), RR1 was shown to detect the presence of as low as

227 , and fluorescence correlation spectroscopy (FCS), to monitor the diverse biophysical states of expan

228 t and fluorescence correlation spectroscopy (FCS), we demonstrated high-affinity labeling of the acti

229 M and fluorescence correlation spectroscopy (FCS), we show that fibril disassembly at pH 6.4 results

230 ed by fluorescence-correlation spectroscopy (FCS), where intensity fluctuations in a small spot are r

231 using fluorescence correlation spectroscopy (FCS), which enables us to monitor the salt-induced compa

232 ed on fluorescence correlation spectroscopy (FCS), which measures antibody-virion binding with all re

233 ed by fluorescence correlation spectroscopy (FCS).

234 ce by fluorescence correlation spectroscopy (FCS).

235 ecule fluorescence correlation spectroscopy (FCS).

236 ed by fluorescence correlation spectroscopy (FCS).

237 using fluorescence correlation spectroscopy (FCS).

238 ue in fluorescence correlation spectroscopy (FCS).

239 using fluorescence correlation spectroscopy (FCS).

240 olved fluorescence correlation spectroscopy (FCS).

241 -fluorescence correlation spectroscopy (SPIM-FCS), a multiplexed modality of FCS, which generates map

242 The FCS diffusion law was applied to SPIM-FCS data to study the subresolution membrane organizatio

243 sSTED-FCS reveals transient molecular interaction hotspots for

244 ation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal heterogene

245 Standard FCS equations are derived from a simple Gaussian approxi

246 has shown great potential for enhancing STED-FCS, but has also created a demand for software which is

247 correlation of point data derived from STED-FCS experiments.

248 with super-resolution STED microscopy (STED-FCS).

249 hojnacki et al. employ super-resolution STED-FCS microscopy to study dynamics of Env molecules on HIV

250 ence correlation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal

251 fluorescence correlation spectroscopy (STED-FCS) to access and compare the diffusion characteristics

252 fluorescence correlation spectroscopy (STED-FCS), a technique which allows the study of membrane dyn

253 ip between functional connectivity strength (FCS) and rCBF during resting and an N-back working-memor

254 In the present study, FCS and PCH were applied to determine the diffusion coef

255 differences for the foveal central subfield (FCS) and total macular volume were computed.

256 r comparison of the foveal central subfield (FCS) volume, total volume, and mean FCP thickness showed

257 blished fluorescence fluctuation techniques (FCS, FCCS, and PCH) required fractions between 7 and 11%

258 We show by simulations and experiments that FCS can sensitively quantify the flow-rates, variability

259 The discrepancy arises from the fact that FCS and FRAP report on different effective (concentratio

260 These data show that FCS analysis of ligand-occupied receptors provides a uni

261 The FCS data in combination with the simulations indicate th

262 The FCS diffusion law was applied to SPIM-FCS data to study

263 The FCS showed a striking spatial correlation with rCBF, and

264 The FCS technique has been significantly advanced by its com

265 ing sites to show that both the FRAP and the FCS estimates may be correct and compatible with the obs

266 s the simplest hypothesis that describes the FCS data based on sampling and signal limitations, natur

267 The results of the FCS analyses and the calculated pristine in-gel diffusio

268 PCH analysis of the FCS data were best described by a one-component dimer mo

269 of the nucleus, improper calibration of the FCS focal volume, or the intentional FRAP photobleach.

270 egration time has an important effect on the FCS autocorrelation function and demonstrate its effect

271 We find that the FCS method allows us to reliably monitor in real-time [m

272 to determine membrane heterogeneity via the FCS diffusion laws, showing that there is a lower length

273 copy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP

274 This FCS-based technique establishes a framework for minimall

275 ng a TAT of 60 minutes or less or an on-time FCS was awarded 1 point.

276 as "wheels out" to "wheels in," and on-time FCS, which was defined as "wheels in" within 6 minutes o

277 had higher mean 18-h PR (p < 0.0001) and TIP-FCS (p = 0.002).

278 Future studies of PR and TIP-FCS for elective stenting may facilitate personalized an

279 the relation of platelet aggregation and TIP-FCS to the occurrence of periprocedural infarction.

280 alirudin on platelet reactivity (PR) and TIP-FCS, and their relation to periprocedural infarction in

281 ion; p < 0.001 for all) and reduced mean TIP-FCS (p < 0.05).

282 The PR, TIP-FCS, and myonecrosis markers were serially determined.

283 n-induced platelet-fibrin clot strength (TIP-FCS) measured by thrombelastography in percutaneous coro

284 agonists and the tensile strength of the TIP-FCS, 2 measurements strongly associated with periprocedu

285 TIR-FCS is also an extremely promising method for studying d

286 steps necessary to construct and test a TIR-FCS system using either through-prism or through-objecti

287 n-fluorescence correlation spectroscopy (TIR-FCS) is an emerging technique that is used to measure ev

288 Our procedure is generally applicable to FCS and image correlation spectroscopy and therefore pro

289 We describe a novel numerical approach to FCS that circumvents conventional analytical models, ena

290 e have been a number of recent extensions to FCS based on laser scanning microscopy.

291 orter than the time required for traditional FCS analysis at each pixel.

292 Jxc1 encodes a nuclear protein that has two FCS-type zinc finger domains (PS51024) and bears nuclear

293 se quantification of DNA hybridization using FCS analysis, in which the FBKs play a major role rather

294 ational diffusion of bioconjugated NRs using FCS might prove to be useful for observing binding and c

295 Spot variation FCS was in accordance with a model of fast microscopic d

296 P may represent an adequate alternative when FCS is unavailable.

297 ection procedure to overcome this issue with FCS data analysis.

298 CBD01 and I-BET762 prior to stimulation with FCS and TGF-beta.

299 ime, which are found to match very well with FCS results.

300 In this work, FCS is used to monitor the dynamics of fluorescence emis