ライフサイエンスコーパス: FRET

1 FRET analysis in vitro is consistent with formation of a

2 FRET and BRET approaches are well established for detect

3 FRET is an indispensable experimental tool for studying

4 FRET theory dramatically underestimated the observed ene

5 FRET was most pronounced for 21 nm-sized UCNPs, yielding

6 FRET-based kinetic measurements were also consistent wit

7 FRET-based stopped-flow measurements revealed that Atg18

8 FRET-estimated radii of gyration and hydrodynamic radii

9 or single-molecule FRET spectroscopy with 2D FRET efficiency-lifetime analysis to probe the oligomeri

10 A FRET-based assay that monitored Aha1 binding to Hsp90 en

11 A FRET-type mechanism was identified in architectures with

12 Using electrophysiology and a FRET-based cAMP assay, two compounds are identified as p

13 e fluorescence emission intensity of CQDs, a FRET-based sensing platform for OPs determination was es

14 Here, we develop a FRET-based methodology to assess the thermodynamics of h

15 We developed a FRET-based assay for detection of CRISPR-Cas9 complex bi

16 in vascular smooth muscle cells expressing a FRET-biosensor comprising the cGMP-binding sites of PKGI

17 inked PLC-beta does not bind Gbetagamma in a FRET-based Gbetagamma-PLC-beta binding assay.

18 ic interactions were monitored by means of a FRET-based sensor of conformation at the allosteric site

19 ecific allosteric inhibitor of Akt through a FRET-based high-throughput screening, and characterizati

20 For the first time, a FRET assay in PCa cells shows Trp-quenching due to Trp-N

21 Using a FRET-based DORA Rac1 biosensor, we show that local Rac1

22 In this work, utilizing a FRET-based method, we have developed a nanobiosensor for

23 restriction enzyme Ecl18kI, interacts with a FRET pair-labeled DNA fragment to form two different DNA

24 pronounced for 21 nm-sized UCNPs, yielding a FRET efficiency of 60%.

25 sorption of qAnitro and thus enable accurate FRET-measurements over more than one turn of B-DNA.

26 ase FRET repertoire with an adenine analogue FRET-pair.

27 e shown previously for our cytosine analogue FRET-pair, FRET between qAN1 and qAnitro positioned at d

28 lights the synergy in molecular dynamics and FRET-based approaches to dissect the structural basis of

29 g simultaneous measurements of extension and FRET during opening and closing of a DNA hairpin under t

30 cal inhibition, genetic loss of function and FRET studies, we show that ENb-TRAIL blocks EGFR signall

31 have applied super-resolution microscopy and FRET to determine the nanoscale spatial organization of

32 uorescence recovery after photobleaching and FRET corroborate the formation of multienzyme metabolic

33 ltiscale MD simulation-based predictions and FRET sensor-based experiments, we investigated the confo

34 Here, using proteomic and FRET analyses, we demonstrate that the ER protein membra

35 rometry, ion mobility mass spectrometry, and FRET.

36 This and FRET confirm that flexibility is interrogated early duri

37 d in an experiment (i.e. the "total apparent FRET efficiency") on the interoligomeric FRET due to ran

38 tly interpreting the measured total apparent FRET efficiency.

39 ts tagged with fluorescent proteins that are FRET pairs exhibit robust energy transfer at the plasma

40 our base analogues can now measure base-base FRET between 3 of the 10 possible base combinations and,

41 Here, we expand the base-base FRET repertoire with an adenine analogue FRET-pair.

42 unoprecipitation (coIP) and anisotropy-based FRET (AFRET) assays confirmed this interaction.

43 antum dots-Gold nanoparticle (QDs-GNP) based FRET probes involving turn on/off principles have gained

44 acceptor, which is useful in lifetime-based FRET experiments.

45 m-triggered release, reported by a Syx-based FRET probe, is abolished upon charge neutralization of 5

46 We find that the relationship between FRET and two-photon intensity quantitatively agrees with

47 Both FRET and PET measurements show that internal friction do

48 the dyes is outside the range accessible by FRET.

49 ament than NM-IIA and -IIC1 as determined by FRET analysis both at cell and bleb cortices.

50 ting complex association and dissociation by FRET microscopy.

51 nce intensity of the organic dyes excited by FRET was comparable to that of the upconversion emission

52 rotein tethered by an ER/K linker flanked by FRET probes.

53 virus isolation in eggs while the results by FRET-PCR and virus isolation overall matched.

54 aps around all four monomers, as revealed by FRET assays.

55 Using a sensitive live-cell FRET assay, we demonstrated selective interaction betwee

56 We present in vivo single-cell FRET measurements in the Escherichia coli chemotaxis sys

57 Here we combined single-cell FRET measurements with analysis based on the fluctuation

58 Biochemical and cellular FRET studies confirm that the extended state of CaMKIIal

59 hnical approach, single-molecule patch-clamp FRET anisotropy imaging and demonstrate by probing the d

60 inding isotherms while including collisional FRET corrections.

61 uorescence lifetime and two- and three-color FRET efficiencies with corrections for submillisecond ac

62 Specifically, combining FRET-based measurements of membrane coverage with multip

63 Combined CT-FRET configurations with QDs are thus promising for appl

64 Currently, FRET/BRET assays rely on co-expression of GPCR and G pro

65 that the donor and acceptor of our cytosine FRET-pair, tC(O) and tCnitro, can be conveniently combin

66 The designed FRET pair, including the donor, CdSe/ZnS quantum dots (Q

67 g fluorescent RNA base analogue for detailed FRET-based structural measurements, as a bright internal

68 imaging (Q-MSI), a technique that determines FRET efficiency and subcellular donor and acceptor conce

69 by measuring the signal amplification during FRET.

70 First, using dynamic FRET analysis in PC12 cells, we show that CDO occurs fol

71 eraction geometry is such that the efficient FRET is expected for one of these conformations-"antipar

72 mechanism of chain looping remains elusive, FRET experiments in formamide and dimethyl sulfoxide sug

73 g sites were biochemically validated by EMSA-FRET analysis and validated in vivo by ChIP-seq data fro

74 1a dihydropyridine receptor subunit enhanced FRET to the II-III loop, thus indicating that beta1a bin

75 eases FRET signal comparable to co-expressed FRET/BRET sensors.

76 Here, using VA-TIRFM and FLIM-FRET, we revealed that AtHIR1 is present in membrane mic

77 sured by fluorescence lifetime imaging (FLIM-FRET) and identified interaction between aquaporin-1 and

78 biosensor using the phasor approach to FLIM-FRET.

79 line HEK 293, live-cell imaging, and Forster/FRET.

80 nt (IQF) peptide substrates originating from FRET (Forster Resonance Energy Transfer) are powerful to

81 er jejuni CmeB pump combined with functional FRET assays to propose a transport mechanism where each

82 Furthermore, FRET and intensity measurements change as expected with

83 and kinetics of PLC-beta3 binding to Galphaq FRET and fluorescence correlation spectroscopy, two phys

84 es in the myocytes using a fourth generation FRET cAMP sensor.

85 y as discrete transitions to a state of high FRET efficiency.

86 ells, and CFP-MDA5:YFP-LGP2 cells had higher FRET efficiencies in the presence of poly(I:C), indicati

87 uorescence correlation spectroscopy and homo-FRET analysis was used to characterize assembly mutants

88 tering subunit stoichiometry or the net homo-FRET between Venus-tagged catalytic domains.

89 ns and chickens from LBM showed that pan-IAV FRET-PCR had a higher detection limit than virus isolati

90 on, and by observing simultaneous changes in FRET and torque during a transition between right-handed

91 discover unanticipated continuous changes in FRET with applied torque, and also show how FluoRBT can

92 s increase in vesicle number and decrease in FRET intensity, indicative of a Src-mediated conformatio

93 Furthermore, a drop in FRET efficiency between YFP and mCherry because of cleav

94 arge set of GPCRs to be used for instance in FRET-based binding assays.

95 litates significantly enhanced resolution in FRET structure determinations, demonstrated here in a st

96 terial lipopolysaccharide leads to increased FRET of fluorescently labeled syntaxin 4 with VAMP3 spec

97 mulation of beta2-AR and alpha2-AR increases FRET signal comparable to co-expressed FRET/BRET sensors

98 HNO induced FRET changes similar to those elicited by an increase of

99 Our intermolecular FRET measurements in living cells are consistent with be

100 ent FRET efficiency") on the interoligomeric FRET due to random proximity within the bilayer and the

101 This interoligomeric FRET (also known as stochastic, bystander, or proximity

102 Intramolecular FRET measurements indicate that, relative to wild-type c

103 netic theory-based model for intraoligomeric FRET, derived in 2007.

104 y within the bilayer and the intraoligomeric FRET resulting from protein-protein interactions.

105 Here, we used transition metal ion FRET and patch clamp fluorometry with a fluorescent, non

106 Combining transition metal ion FRET, patch-clamp fluorometry, and incorporation of a fl

107 erin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility with

108 ich promoted a shift of bound CaM to a lower FRET orientation (without altering the amount of CaM bou

109 hondria in CFP-LGP2:YFP-LGP2 cells had lower FRET signal in the presence of poly(I:C), suggesting tha

110 Combining MC-FRET with solid-state wide-line and high resolution magi

111 rgy transfer and Monte Carlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liq

112 There are a number of methods to measure FRET.

113 proaches, our method correlates the measured FRET efficiencies to relative concentration of interacti

114 ra to the standard deviation of the measured FRET efficiency.

115 ical performance was determined by measuring FRET efficiency and photostability of tandem fusion prot

116 er-fluorescence lifetime imaging microscopy (FRET-FLIM)-based technique allowing visualization of rea

117 Single molecule FRET analysis in vitro, combined with MD simulations, sh

118 Novel single molecule FRET binding and unwinding assays show an interaction of

119 ts, we then predict incisive single molecule FRET experiments as a means of model validation.

120 Using a novel single molecule FRET system, we found that Spt4/5 affected the kinetics

121 Here, we use three-color single molecule FRET to show how combinations of ribosomal proteins uS4,

122 Here we employed single molecule FRET, single molecule pull-down and biochemical analysis

123 racteristics with respect to single-molecule FRET (smFRET) data.

124 We here report microsecond single-molecule FRET (smFRET) measurements on Cy3/Cy5-labeled primer-tem

125 Here, using single-molecule FRET (smFRET), we show that prior to ligation, differenc

126 resolution, integrated with single-molecule FRET analysis and in vitro biochemical assays.

127 of X-ray crystallography and single-molecule FRET analysis to reveal the interactions of distinct cla

128 , we developed a three-color single-molecule FRET assay to study the interaction of Hsp90 with a fluo

129 Here, single-molecule FRET experiments on freely diffusing TFAM/LSP complexes

130 ranslocation mechanism using single-molecule FRET has led to the hypothesis that substrate movements

131 d evanescent scattering, and single-molecule FRET imaging, providing real-time multiparameter measure

132 These solution-based single-molecule FRET measurements of a multimeric ion channel in a lipid

133 tent with these predictions, single-molecule FRET measurements of folding of model RNAs revealed cons

134 However, by using single-molecule FRET spectroscopy that includes measurements of fluoresc

135 ombines two- and three-color single-molecule FRET spectroscopy with 2D FRET efficiency-lifetime analy

136 we conducted confocal-based single-molecule FRET studies to investigate this phenomenon in greater d

137 We here use single-molecule FRET techniques to build on previous thermodynamic studi

138 the authors use three-color single-molecule FRET to show how the dynamics of the rRNA dictate the or

139 Using single-molecule FRET, we now show that the analogous Escherichia coli (E

140 chain libraries suitable for single-molecule FRET-based conformational phenotyping.

141 l new pairs were applied in a multimolecular FRET based sensor for detecting activation of a heterotr

142 In this study we use multiple FRET-based reporters for the detection of cAMP and PKA a

143 t and accurate detection, the nanostructured FRET sensors were assembled onto a patterned ZnO nanorod

144 ental data, such as several NMR observables, FRET, SAXS and cryo-electron microscopy data, and enable

145 Simultaneous administration of FRET donor and acceptor anti-PrP(C) antibodies to living

146 t in two dimensions, a substantial amount of FRET is generated by energy transfer between fluorophore

147 Simultaneous analysis of FRET and acceptor intensity trajectories then allows us

148 immune cells and prove the applicability of FRET-FLIM for visualizing SNARE complexes in live cells

149 In this article, applications of FRET microscopy to protein interactions and modification

150 cent moieties, we generated a novel class of FRET-based reporter to monitor conformational difference

151 es can also be modulated by a combination of FRET and charge transfer (CT), and characterize the conc

152 Here, we utilize a combination of FRET and molecular dynamics (MD) simulations to probe th

153 Through a combination of FRET and single molecule studies, we find that the incre

154 bleed-through (SBT) and the confirmation of FRET measurements from the known standards.

155 Development of FRET-quenched substrates for exo-glycosidases, however,

156 The concurrent effects of FRET and CT were predictable from a rate analysis that w

157 Our results suggest that the effects of FRET and photoredox quenching should be taken into consi

158 ign, synthesis, and biological evaluation of FRET Iron Probe 1 (FIP-1), a reactivity-based probe that

159 f CT quenching, approximately independent of FRET.

160 ET, that enables quantitative measurement of FRET efficiency.

161 -photon microscopy with FLIM measurements of FRET.

162 Fluorescence microscopy of FRET-based biosensors allow nanoscale interactions to be

163 Furthermore, occurrence of FRET from the donor (fluorene) to acceptor (BT units), v

164 io reflected changes in the relative rate of FRET but was approximately independent of CT.

165 fferent ratios, tuning the relative rates of FRET and CT, which were competitive quenching pathways.

166 of Au NPs and the corresponding recovery of FRET-quenched fluorescence emission.

167 Here we improved the sensitivity of FRET-based kinase sensors for monitoring kinase activity

168 ) and live-cell imaging including the use of FRET-based Rho GTPase biosensors.

169 can be analysed by techniques such as NMR or FRET, provided that the information relative to the indi

170 Our FRET, biochemical and EPR analysis suggests that this fu

171 AF heterodimer, we have combined single-pair FRET and NMR experiments.

172 We used a single-pair FRET technique to examine the effect of a GXXXG motif on

173 viously for our cytosine analogue FRET-pair, FRET between qAN1 and qAnitro positioned at different ba

174 photoluminescence intensity of the patterned FRET sensor increases linearly with increasing concentra

175 onstrate the quick response of the patterned FRET sensor to 2microL of tear samples.

176 nd Cerulean-Venus FRET standards as positive FRET controls.

177 pt the CRAC domains of both proteins prevent FRET between SIDT1 and SIDT2 and the cholesterol analogu

178 known as stochastic, bystander, or proximity FRET) is not accounted for in either model.

179 odels without consideration of the proximity FRET leads to incorrect conclusions about the oligomeric

180 Typically, the QD-FRET constructs have made use of labeled targets or have

181 e of membrane proteins in a static quenching FRET experiment: the model of Veatch and Stryer, derived

182 find that bulk, two-color, static quenching FRET experiments are best suited for the study of monome

183 meaningful parameters from static quenching FRET measurements in biological membranes.

184 1-phosphate (i.e., transient alpha1B-AR-Rab5 FRET signal followed by a sustained alpha1B-AR-Rab9 inte

185 wed the highest dynamic range in ratiometric FRET imaging experiments with the G-protein sensor.

186 w how FluoRBT can facilitate high-resolution FRET measurements of molecular states, by using a mechan

187 rectly by performing transient time-resolved FRET on a ventricular cardiac myosin biosensor.

188 Here the authors use time-resolved FRET to measure binding kinetics, and show that side eff

189 Application of the pan-IAV RT FRET-PCR to oral-pharyngeal and cloacal swab specimens c

190 The detection limit of one-step pan-IAV RT FRET-PCR was 10 copies of the matrix gene per reaction,

191 Furthermore, similar FRET efficiencies for FT-FD and AcMFT-FD heterodimer in

192 of beta2 subunits; and both yielded similar FRET profiles when probed for subunit adjacency, suggest

193 optimized for high dynamic range and stable FRET signals.

194 ewer approaches using subcellularly targeted FRET reporter sensors have helped define more compartmen

195 The FRET changes observed in Escherichia coli are more compl

196 The FRET signal could be diminished by the further addition

197 troduce dyes in proximity to the QDs for the FRET process.

198 formalism to describe the dependence of the FRET efficiency measured in an experiment (i.e. the "tot

199 leavage by NE results in dissociation of the FRET fluorescent protein pair and alteration of the fluo

200 tribute to the detailed understanding of the FRET-based biosensor and guide the rational design of ne

201 At larger surface-to-volume ratios, the FRET efficiency decreased by an increasing competition o

202 with two different activation reporters, the FRET-based calcium biosensor Twitch1 and fluorescent NFA

203 Surprisingly, the FRET efficiency was lower for the bound IkappaBalpha mol

204 se, the quenched emission of QDs through the FRET mechanism is restored by displacing the dextran fro

205 Using the FRET approach and in vitro phosphodiesterase (PDE) activ

206 The properties of these FRET-based constructs will also allow further studies of

207 Furthermore, the sensitivity of this FRET strategy amplified using AuNPs/graphene oxide nanoc

208 Three FRET-substrates (Abz-GFY-pNA, Abz-SFY-pNA and Abz-GFI-pN

209 The probe detects the bilirubin through FRET mechanism.

210 fluorescent dye that quenched QD PL through FRET or a ruthenium(II) phenanthroline complex that quen

211 d genetic approaches combined with real-time FRET imaging and high resolution microscopy, we demonstr

212 in conformation, making it complementary to FRET based techniques, which are insensitive at very sho

213 ved (TR) small-angle X-ray scattering and TR-FRET to correlate changes in the DNA conformations with

214 e now report that the same conformational TR-FRET based immunoassay detects polyglutamine- and temper

215 d fluorescence resonance energy transfer (TR-FRET) and double electron-electron resonance (DEER), com

216 d fluorescence resonance energy transfer (TR-FRET) assay was 9.6 ng/mL, and the limit of detection (L

217 d Fluorescence Resonance Energy Transfer (TR-FRET) assay, we demonstrate that Munc13-4 binds to Rab11

218 d fluorescence resonance energy transfer (TR-FRET) technology, to identify reversible inhibitors.

219 d in-cell Forster resonance energy transfer (FRET) and glutathione S-transferase pulldown analyses id

220 cribed to Forster resonance energy transfer (FRET) and, to a lesser extent, nanosurface energy transf

221 ntitative Forster resonance energy transfer (FRET) approach to show that Ecadherin forms constitutive

222 ement and forster resonance energy transfer (FRET) approach.

223 d on fluorescence resonance energy transfer (FRET) are powerful tools for quantifying and visualizing

224 time-resolved fluorescence energy transfer (FRET) assay reporting membrane expression and real-time

225 cytometry-Forster resonance energy transfer (FRET) assay.

226 and fluorescence resonance energy transfer (FRET) assays, we now show the PBM directs interaction of

227 tive Fluorescence Resonance Energy Transfer (FRET) based assay.

228 t of fluorescence resonance energy transfer (FRET) between phycoerythrin-biotin (PhycoE-Biotin) and C

229 (or fluorescence) resonance energy transfer (FRET) between the C-dots and EtBr was studied, in which

230 ng a fluorescence resonance energy transfer (FRET) biosensor, we show that a phosphomimetic mutation

231 rmance of Forster Resonance Energy Transfer (FRET) biosensors depends on brightness and photostabilit

232 red (NIR) Forster resonance energy transfer (FRET) both in vitro and in vivo.

233 d on Fluorescence Resonance Energy Transfer (FRET) for the MPR by employing computational simulation

234 on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluor

235 -molecule Forster resonance energy transfer (FRET) is a powerful tool to study interactions and confo

236 Forster resonance energy transfer (FRET) is a versatile method for analyzing protein-protei

237 , we used Forster resonance energy transfer (FRET) measured by fluorescence lifetime imaging (FLIM-FR

238 cule fluorescence resonance energy transfer (FRET) methods to investigate a set of twister RNAs with

239 ethod and Forster resonance energy transfer (FRET) microscopy imaging to visualize nanoparticle self-

240 Forster resonance energy transfer (FRET) microscopy is a powerful technique capable of inve

241 y encoded Forster resonance energy transfer (FRET) nanosensors.

242 t a simple Forster resonant energy transfer (FRET) network model accurately predicts the observed pho

243 as either Forster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor.

244 equential Forster resonance energy transfer (FRET) pathways between QDs and fluorescent dyes, lumines

245 ions, two Forster resonance energy transfer (FRET) populations were observed that corresponded to a f

246 ular fluorescence resonance energy transfer (FRET) probe, which previously allowed us to resolve a de

247 cence and Forster resonance energy transfer (FRET) processes by fixing the fluorophores in a rotaxane

248 sing fluorescence resonance energy transfer (FRET) quenching mechanism.

249 d using a Forster Resonance Energy Transfer (FRET) relationship between the photochrome and a co-enca

250 g of fluorescence resonance energy transfer (FRET) signals between CDs and AuNPs as nanoquenchers, th

251 Forster resonance energy transfer (FRET) studies performed at the single molecule level hav

252 -molecule Forster resonance energy transfer (FRET) to measure the global reconfiguration dynamics of

253 with fluorescence resonance energy transfer (FRET) to resolve single-molecule association dynamics at

254 ree-color Forster resonance energy transfer (FRET) tracking methods.

255 Forster resonance energy transfer (FRET) using fluorescent base analogues is a powerful mea

256 ns, using Forster resonance energy transfer (FRET), confocal microscopy, and intracellular calcium qu

257 nation of Forster resonance energy transfer (FRET), nonreducing SDS-PAGE, and strategic mutation of t

258 vivo fluorescence resonance energy transfer (FRET), small-angle x-ray scattering (SAXS), x-ray crysta

259 sing fluorescence resonance energy transfer (FRET), we demonstrate increased interaction of the hVDR-

260 d on fluorescence resonance energy transfer (FRET), which can detect the real-time PDGFR activity in

261 cule fluorescence resonance energy transfer (FRET), which enabled us to resolve heterogeneous populat

262 th a fluorescence resonance energy transfer (FRET)-based alpha-catenin conformation sensor demonstrat

263 s of fluorescence resonance energy transfer (FRET)-based beta-arrestin2 biosensors.

264 oyed fluorescence resonance energy transfer (FRET)-based biosensors in conjunction with collagen-coup

265 eted fluorescence resonance energy transfer (FRET)-based biosensors toward subcellular FAs to report

266 targeted Forster resonance energy transfer (FRET)-based calcium indicator (4mtD3cpv, MitoCam) was ex

267 ressing a Forster resonance energy transfer (FRET)-based cAMP biosensor, we confirmed that atropine i

268 a set of Forster resonance energy transfer (FRET)-based crowding-sensitive probes and investigate th

269 te a fluorescence resonance energy transfer (FRET)-based ratiometric biosensor array.

270 nt a fluorescence resonance energy transfer (FRET)-based sensor, CUTie, which detects compartmentaliz

271 hile fluorescence resonance energy transfer (FRET)-based sensors for these kinases had previously bee

272 tion fluorescence resonance energy transfer (FRET)-PCR.

273 Tunable Forster resonance energy transfer (FRET)-quenched substrates are useful for monitoring the

274 via fluorescence resonance energy transfer (FRET).

275 s in fluorescence resonance energy transfer (FRET).

276 hint for Forster resonance energy transfer (FRET).

277 based on Forster resonance energy transfer (FRET).

278 ed on fluorescence resonance energy transfer(FRET) between carbon dots(CDs) and AuNPs as nanoquencher

279 To this end, we excite two FRET donors mTFP1 and LSSmOrange with a 440 nm wavelengt

280 e variants on TRIO function, we utilized two FRET-based biosensors: a Rac1 biosensor to study mutatio

281 We now use FRET measurements of pathway activity, analysis of prote

282 We used FRET-FLIM to delineate the trafficking steps underlying

283 Using FRET between cerulean fluorescent protein-labeled Gbetag

284 In addition, using FRET-based FLIM approach, we demonstrate that D-serine r

285 Here, comprehensive analysis using FRET-based imaging reveals that activity-driven and Prot

286 s in the same cellular localization by using FRET biosensors.

287 tric kinase biosensing in living cells using FRET-FLIM.

288 PLSM system is evaluated and optimized using FRET standards expressed in living cells, which enables

289 o demonstrate the versatility of sFISH using FRET detection and mRNA isoform profiling as examples.

290 s as a zero FRET control, and Cerulean-Venus FRET standards as positive FRET controls.

291 Using a quantitative in vitro FRET assay, we show that gammaTuRC assembly is criticall

292 Ex-vivo FRET imaging of mouse cartilage explants showed that int

293 s technique exploits the principle that when FRET occurs, energy from a donor fluorophore is transfer

294 Escherichia coli are more complicated, where FRET-increases and scaling behavior are observed solely

295 g increased SNARE complex formation, whereas FRET with other tested SNAREs was unaltered.

296 Here, we evaluated whether FRET-based biosensors provide sufficient contrast and sp

297 Despite this, when assayed with FRET tau biosensor cells, extracellular vesicles derived

298 In combination with FRET it allowed us to reveal and characterize the dynami

299 measurements of global DNA deformations with FRET measurements of local conformational changes, FluoR

300 Fluorescein and TAMRA dye mixtures as a zero FRET control, and Cerulean-Venus FRET standards as posit