ライフサイエンスコーパス: Fluo-3

1 a-2, fura-2) or diminished (magnesium green, fluo-3).

2 inding sites and a diffusible indicator dye (fluo-3).

3 of the single wavelength calcium indicator (Fluo-3).

4 croscopy with the fluorescent Ca2+ indicator fluo 3.

5 lmodulin along with the Ca(2+)-sensitive dye fluo 3.

6 with mock intracellular solutions containing fluo 3.

7 frequency (CaSpF) by confocal microscopy and fluo 3.

8 s loaded with the fluorescent Ca2+ indicator fluo-3.

9 ed with patch pipettes containing 0.1 microM fluo-3.

10 es: mag-fura-2, magnesium green, fura-2, and fluo-3.

11 icator Oregon Green 488 Bapta 5N in place of fluo-3.

12 icroscopy and the fluorescent Ca2+ indicator fluo-3.

13 oaded with the fluorescent calcium indicator fluo-3.

14 hair cells dialyzed with the Ca2+ indicator fluo-3.

15 ae micro-injected with the calcium indicator fluo-3.

16 microscopy and the fluorescent calcium probe fluo-3.

17 aging with the fluorescent calcium indicator fluo-3.

18 fluorometry using the calcium-sensitive dye, Fluo-3.

19 aded with the fluorescent calcium indicator, fluo-3.

20 the SR, and cytosolic Ca2+ was imaged using fluo-3.

21 ase from the SR was detected using Fura-2 or Fluo-3.

22 based on the use of a micropipet filled with fluo-3.

23 s loaded with the fluorescent Ca2+ indicator fluo-3.

24 g confocal microscopy and the Ca2+ indicator fluo-3.

25 ed by confocal measurements of [Ca2+]i using fluo-3.

26 Ca(2+) transients were measured with fluo-3.

27 free calcium by using the calcium indicator fluo-3.

28 oaded with the fluorescent Ca(2+) indicator, fluo-3.

29 ileum were loaded with the Ca(2+) indicator fluo-3.

30 5 mmol/L) and the fluorescent Ca2+-indicator fluo-3 (1 mmol/L), depolarization from -40 to 0 mV elici

31 Fluo-3 (100 microM) significantly "shrinks" the spark.

32 Fluo-3, (2) both the level of intracellular cations and

33 aminofluorescein diacetate, an NO probe, and Fluo-3, a Ca(2+) probe.

34 d luciferin for the ATP assay or loaded with Fluo-3-acetoxy methylester for intracellular calcium mea

35 were loaded with the calcium indicator dye, fluo-3 acetoxymethyl ester, and fluorescence was measure

36 Cs were loaded with a calcium indicator dye, fluo-3 acetoxymethyl ester, and the fluorescence was mea

37 Single smooth muscle cells were loaded with fluo-3 acetoxymethyl ester, and the fluorescence was rec

38 Monocyte-like U937 cells were labeled with fluo-3-acetoxymethyl ester to quantitate intracellular C

39 d with simultaneous measurements of [Ca2+]i (fluo-3-acetoxymethyl ester) and membrane currents/action

40 thelium were loaded with the fluorescent dye fluo-3 AM and changes in intracellular calcium concentra

41 , we loaded ORNs with the Ca2+ indicator dye Fluo-3 AM and measured fluorescence with a laser scannin

42 Calcium imaging with fluo-3 AM and morphological analyses after exposure to g

43 Simultaneous measurement of cytosolic (fluo-3 AM) and mitochondrial (rhod-2 AM) Ca2+ responses

44 hose observed in intact myocytes loaded with fluo-3 AM.

45 Using the Ca2+-sensitive fluorescent dye, Fluo-3, AM, and a trypan blue exclusion assay, we evalua

46 d using the cell-permeable calcium indicator Fluo-3-AM, and the globulization time (T(g)) was determi

47 with the membrane-permeant Ca(2+) indicator fluo-3/AM and subsequent removal of cytoplasmic fluo-3 b

48 Cells were labeled with fluo-3/AM to quantitate intracellular Ca2+ ([Ca2+]i) by

49 of normal canine left ventricle, loaded with Fluo-3/AM, and studied in normal Tyrode's solution (24 d

50 scence of an entrapped Ca(2+)-sensitive dye, Fluo-3/AM.

51 Transient increases in both cytosolic Fluo 3 and mitochondrial Rhod 2 fluorescence occurred af

52 When myocytes were loaded with 1 mM fluo-3 and 3 mM EGTA via the patch pipette to buffer dia

53 3]2+ as a pH sensor and of the calcium probe Fluo-3 and [Ru 2,2'-(bipyridyl)3]2+ as a calcium sensor.

54 aged simultaneously using the Ca2+ indicator fluo-3 and a confocal microscope.

55 The fluorescent dyes Fluo-3 and Calcium Green-5N were used to monitor local C

56 es were investigated with the Ca2+ indicator fluo-3 and confocal and two-photon microscopy.

57 l cells using the fluorescent Ca2+ indicator fluo-3 and confocal laser scanning microscopy.

58 entricular cells using the calcium indicator fluo-3 and confocal microscopy.

59 Ca2+ was monitored using fluo-3 and confocal microscopy.

60 he sarcoplasmic reticulum were detected with fluo-3 and confocal scanning microscopy.

61 lular cation levels by using the fluorophore Fluo-3 and exposure of PS on the outer surface of the RB

62 ggesting a competition between intracellular Fluo-3 and extracellular EGTA.

63 using real time fluorescent microscopy with Fluo-3 and fluorometry with Fura-2.

64 esters of Fluo-3 or Calcium Green-2, or with Fluo-3 and Fura Red, and changes in [Ca2+]i of single co

65 Intracellular calcium, measured using Fluo-3 and Fura-2 fluorescent calcium indicator dyes, wa

66 r microscopy with the calcium-sensitive dyes fluo-3 and fura-2 were used to study the influence of GA

67 pal neurons filled with the fluorescent dyes Fluo-3 and Fura-red, that were intermittently excited by

68 aded with the Ca2+-sensitive fluorescent dye Fluo-3 and imaged by a digital epifluorescence imaging s

69 rded using the intracellular Ca2+ indicators fluo-3 and indo-1 while action potentials (APs) or membr

70 ated rat ventricular myocytes measured using fluo-3 and laser scanning confocal microscopy.

71 etermined by stopped-flow spectroscopy using fluo-3 and mag-fura-2AM, respectively.

72 rature using the fluorescent Ca2+ indicators fluo-3 and Oregon Green 488 Bapta 5N.

73 Fluo-3 and propidium iodide were employed to evaluate ca

74 Spontaneous Ca(2+) sparks were detected with fluo-3 and single photon confocal microscopy; mitochondr

75 CCs after loading with the calcium indicator fluo-3 and were associated with depolarizations of the I

76 ere recorded using a confocal microscope and Fluo-3 and were quantified considering missed events.

77 s muscles were loaded with Ca2+ fluorophore (Fluo-3) and caged Ca2+ (dimethoxynitrophenamine or o-nit

78 c reticulum (ER) ([Ca(2+)](L)), using high- (Fluo-3) and low- (Mag-Fura-2) affinity Ca(2+)-sensitive

79 g as an index of contractility, [Ca2+]i with fluo 3, and pHi with seminaphthorhodafluor-1 (SNARF-1).

80 In granule cells loaded with fluo-3, ArgTX-636 and philanthotoxin-343 (PhTX-343) anta

81 red by confocal laser-scanning microscopy of fluo-3 at video rates, in fast twitch muscle fibres, sti

82 o-3/AM and subsequent removal of cytoplasmic fluo-3 by surface membrane permeabilization with digiton

83 trocyte cultures was performed together with Fluo-3-Ca imaging at millisecond temporal resolution and

84 We used fura-2 and fluo-3 Ca2+ digital imaging to assess the effects of inh

85 olarizations to positive potentials elicited fluo-3 Ca2+ transients with rates of rise that were line

86 We used confocal microscopy to compare fluo-3 [Ca2+]i transients in the subsarcolemmal space an

87 easured using the fluorescent Ca2+ indicator fluo-3 combined with laser scanning confocal microscopy.

88 ation of Ca2+ buffers (2-5 mM EGTA plus 1 mM fluo-3) completely abolished the I(Ca)-gated propagation

89 calcium concentration ([Ca2+]i) transients (fluo-3, confocal microscopy), fractional shortening (vid

90 done by 1) simulating Ca2+ release, Ca2+ and fluo-3 diffusion, and Ca2+ binding reactions; 2) simulat

91 ciation constants were measured in vitro for fluo-3, fluo-4 and fluo-5F with and without 1 mM Mg(2+)

92 er segments of intact mouse rods loaded with fluo-3, fluo-4 or fluo-5F, to estimate dark, resting fre

93 , and [Ca2+]i was measured simultaneously as fluo 3 fluorescence using laser scanning confocal micros

94 Fluo-3 fluorescence associated with Ca2+ release was rec

95 Fluo-3 fluorescence associated with Ca2+ release was rec

96 RyR2 location was monitored by imaging fluo-3 fluorescence due to Ca2+ flux through RyR2 channe

97 apable of laser scanning confocal imaging of fluo-3 fluorescence due to Ca2+ flux through single RyR2

98 model, we compared the predicted profiles of fluo-3 fluorescence during the response to mechanical st

99 The fluo-3 fluorescence evoked by progressively increasing o

100 widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions (2D

101 transient measured by confocal line-scans of fluo-3 fluorescence in voltage-clamped cells were 3- to

102 rations both the suction pipette current and fluo-3 fluorescence increased dynamically, rising with a

103 Fluo-3 fluorescence measurements were made in isolated b

104 , and Ca(2+) transients measured by confocal fluo-3 fluorescence of transfected myotubes under whole-

105 saturating RyR2 Ca2+ currents, proportional fluo-3 fluorescence was recorded simultaneously with Ca2

106 Line-scan confocal microscopy (Fluo-3 fluorescence) of intact cardiomyocytes stimulated

107 g and intracellular Ca(2+) transients (using Fluo-3 fluorescence).

108 Similarly, with Fluo-3 fluorescence, BzATP induced an increase in intrac

109 monitored using fast x-y confocal imaging of fluo-3 fluorescence.

110 thin the cells was monitored using Fura-2 or Fluo-3 fluorescence.

111 l, such as Ca2+ buffers and diffusion, alter fluo-3 fluorescent responses to RyR2 Ca2+ currents, and

112 (2+) accumulation using radioimmunoassay and Fluo-3 fluorometry, respectively.

113 Flow cytometry was used with fluo 3 for [Ca(2+)](i) and sodium green for [Na(+)](i) m

114 In LV myocytes loaded with fluo-3, fractional cell shortening and the amplitude of

115 n left ventricular (LV) myocytes loaded with Fluo-3 from NRG-1+/- and wild-type (WT) mice.

116 opular indicators such as Calcium Green-1 or Fluo-3 fulfill these conditions.

117 easured as the ratio of the fluorescences of fluo-3/fura-red by confocal microscopy.

118 ial membrane potential (MMP) was shown using Fluo-3/Fura-red labeling and flow cytometry, and confirm

119 lar Ca(2+), as assayed by the indicator dye, Fluo-3, had similar kinetics and voltage dependence for

120 esponses to glutamate measured with confocal fluo-3 imaging are retained during AMPA/KA receptor bloc

121 and [Ca2+]i were measured with the indicator fluo 3 in myocytes from MI, MI+GH, control, and normal a

122 from salamander using the Ca2+ indicator dye fluo-3 in combination with laser scanning confocal micro

123 oncentration ([Ca(2+)](i)) was measured with fluo-3 in rat ventricular myocytes.

124 Experiments were performed using Fluo-3 in voltage clamped rat ventricular myocytes.

125 We measured [Ca(2+)]i with fluo-3 in voltage-clamped rat ventricular myocytes.

126 oupling, we measured [Ca(2+)](i) transients (fluo-3) in single voltage-clamped mouse ventricular myoc

127 2 into mitochondria and other organelles and Fluo 3 into the cytosol of adult rabbit cardiac myocytes

128 hat the spatial distribution of Ca(2+)-bound fluo-3 is Gaussian, we show the following: 1) variations

129 Confocal imaging of Fluo-3-labeled NM was used to investigate the kinetics o

130 +/-4% versus 166+/-7% of baseline at similar fluo-3 levels, P=0.0004, n=52 and 25, respectively).

131 e applied our model to determine [Ca(2+)] in Fluo-3-loaded bovine aortic endothelial cells (BAECs).

132 In fluo-3-loaded CD19(+) B and DAKIKI cells, 4-chloro-m-cre

133 ored real-time alterations in [Ca(2+)](i) in fluo-3-loaded cerebellar granule neurons exposed to domo

134 llular Ca(2+) concentration was monitored in fluo-3-loaded CGN using a fluorescent laser imaging plat

135 pretreatment abolished Ca(2+) transients in fluo-3-loaded fibres following even prolonged applicatio

136 ted the subcellular origin of Can signals in Fluo-3-loaded HeLa cells.

137 spontaneous release events (Ca2+ sparks) in fluo-3-loaded isolated rat ventricular myocytes.

138 Ca2+ sparks') were observed in about 40 % of fluo-3-loaded myocytes examined using laser scanning con

139 Voltage-clamped, fluo-3-loaded myocytes were studied using confocal micro

140 This method was applied to Fluo-3-loaded neutrophils that were activated by the che

141 with recordings of inward Ca(2+) current in fluo-3-loaded patch-clamped rat ventricular myocytes.

142 pling (E-C coupling) was studied in isolated fluo-3-loaded rat atrial myocytes at 22 and 37 degrees C

143 gregation states and tested their actions on fluo-3-loaded SH-SY5Y cells.

144 Laser scanning confocal microscopy of fluo-3-loaded single myocytes freshly isolated from smal

145 R) activity were induced in fully polarized, fluo-3-loaded, intact frog skeletal muscle fibres by exp

146 in slice preparations and 'intensitometric' fluo-3 measurements of confocal images were used to simu

147 A fluorogenic complex is formed when fluo-3 meets calcium in the bath solution.

148 ortical oligodendrocytes were incubated with fluo 3 or fura 2, and digital video fluorescence microsc

149 were loaded with the acetoxymethyl esters of Fluo-3 or Calcium Green-2, or with Fluo-3 and Fura Red,

150 s were loaded with a fast calcium indicator (Fluo-3 or Fluo-5F) and an excess of a high-affinity but

151 lised cardiomyocytes perfused with 10 microM Fluo-3 or Fluo-5F.

152 ernal' solutions containing: MgATP, EGTA and fluo-3 potassium salt.

153 cells filled with the fluorescent indicator FLUO-3, revealed a transient increase in intracellular C

154 d with the calcium-sensitive fluorescent dye fluo-3 showed no significant change in fluorescence inte

155 ersus RyR-3 null cells measured at rest with fluo-3 showed that neither the peak fluorescence intensi

156 wo-dye ratiometric method using Fura Red and fluo-3, showed a progressive increase in [Ca2+]i in fibe

157 We loaded myocytes with fluo-3 to image Ca2+ sparks using confocal microscopy in

158 We used confocal Ca2+ imaging and fluo-3 to investigate the transition of localized Ca2+ r

159 clamp and line-scan confocal microscopy with fluo-3 to measure intracellular [Ca(2+)] ([Ca(2+)](i)) a

160 and ferret ventricular myocytes loaded with fluo-3 to measure intracellular Ca(2+) concentration ([C

161 buffers and the fluorescent Ca(2+) indicator fluo-3 to produce the model Ca(2+) spark.

162 2 fluorescence transients but not cytosolic Fluo 3 transients.

163 ction) microinjected with the Ca2+ indicator fluo 3 under physiological conditions ([Ca2+]o, 1 mmol/L

164 tes loaded with the Ca2+-sensitive indicator fluo-3, using confocal microscopy at 20-22 C.

165 pressing mouse cardiomyocytes, dialysed with fluo-3, using rapid (120-240 frames s-1) two-dimensional

166 and Meg-01 megakaryocytic cells loaded with Fluo-3 was examined by confocal microscopy.

167 Fluo-3 was used as a Ca2+ indicator in either smooth mus

168 rol V(m) and measure I(m), respectively, and Fluo-3 was used to measure [Ca2+]i in myocytes from nonf

169 with the calcium-sensitive fluorescent probe fluo-3 was used to study spatial aspects of intracellula

170 Confocal microscopy (fluo-3) was used to measure Ca(2+) spark frequency (CaSp

171 pump, and fluorescent Ca(2+)-indicator dye (fluo-3), was developed to numerically simulate laser sca

172 ore, using the calcium indicators Fura-2 and Fluo-3 we show that root intracellular calcium concentra

173 +) and a Ca(++)-sensitive fluorescent probe (Fluo-3), we demonstrated that LPA, but not phosphatidic

174 g the calcium-specific fluorescent indicator fluo-3, we also found that C(2)-ceramide activation of e

175 ([Ca(2+)](i)) with the Ca(2+)-sensitive dye fluo-3, we demonstrated that 10 mM caffeine activated ry

176 ed with the acetoxymethyl ester (AM) form of fluo-3 were imaged at rest or under whole-cell patch cla

177 contraction and [Ca(2+)](i) transients with fluo-3 were measured simultaneously.

178 Confocal microscopy and the Ca2+ indicator fluo-3 were used to visualize the elementary release eve

179 n potentials (patch clamp), and [Ca(2+)](i) (Fluo-3) were measured in right atrial samples from 76 si

180 4) Adding 100 microM fluo-3, which doubles the buffering capacity of the cyto