ライフサイエンスコーパス: Fluoro-Jade

1 We previously showed that Fluoro-Jade, a fluorescent stain that labels injured, de

2 and entorhinal cortex) were evaluated using Fluoro-Jade, a histofluorescent stain for degenerating n

3 pared with non-transgenic counterparts using Fluoro-Jade, a marker for neurodegeneration, both the hi

4 d to examine the exact effects of MCAO using Fluoro-Jade, a marker of neurodegeneration that allows o

5 al injury after traumatic brain injury using Fluoro-Jade, an acidic dye that exhibits a marked affini

6 Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, was found to stain all de

7 To confirm using molecular techniques that Fluoro-Jade and Newport Green-positive neurons are equiv

8 Sections double stained for Fluoro-Jade and NF-kappaB activity (using a mouse engine

9 alize degenerating neurons or neurites using Fluoro-Jade, anti-tau, and anti-alpha synuclein staining

10 Labeling with Fluoro-Jade B (FJB), a fluorescent green dye that labels

11 rom a separate set of mice were stained with Fluoro-Jade B (FJB), a fluorochrome that binds specifica

12 he number of degenerating cells positive for Fluoro-Jade B (FJB+) in the region.

13 iated with neuronal death as visualized with Fluoro-jade B and anti-active caspase-3 staining.

14 Moreover, double labeling with Fluoro-Jade B and glial fibrillary acidic protein (GFAP)

15 ions were stained for nonviable neurons with Fluoro-Jade B and the volume of damaged tissue was estim

16 The development of Fluoro-Jade B as a simple and reliable marker of neurona

17 cal lesion size and cell death analysis with Fluoro-jade B failed to reveal Z-BDDA enhanced neuroprot

18 However, Fluoro-Jade B has an even greater specific affinity for

19 Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other label

20 Fluoro-Jade B labeled neurons were not evident in the RV

21 Treatment with METH shows Fluoro-Jade B positive cell bodies in the striatum and p

22 In contrast, the number of Fluoro-Jade B positive degenerating neurons in the Purki

23 orris Water Maze and a reduction in positive Fluoro-Jade B stained injured neurons and microglial act

24 Degenerating neural cells, detected by Fluoro-Jade B staining and increased protein oxidation,

25 t to the Fluoro-Jade B-positive cells but no Fluoro-Jade B staining of the astrocytes.

26 thology characterized by positive silver and Fluoro-Jade B staining, and microglial proliferation and

27 bsequently assessed by hematoxylin-eosin and Fluoro-Jade B staining.

28 m and pretreatment with WIN-51,708 abolished Fluoro-Jade B staining.

29 tivity (stage 1); however, cupric-silver and Fluoro-Jade B stains revealed significant damage by 12 h

30 parvalbumin (PV), cholecystokinin (CCK), and Fluoro-Jade B was performed on sections from hippocampus

31 In Experiment I, Fluoro-Jade B, an indicant of degenerating cells, reveal

32 Fluoro-Jade B, like its predecessor Fluoro-Jade, is an a

33 de C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, was found to stain all degenerating neuro

34 ive astrocytosis in the area adjacent to the Fluoro-Jade B-positive cells but no Fluoro-Jade B staini

35 Furthermore, neuronal injury, expressed as Fluoro-Jade B-positive cells in the hippocampal formatio

36 -IR and NPY-IR interneurons also stained for Fluoro-Jade B.

37 neuronal damage after MPTP, as evidenced by Fluoro Jade-B staining (to identify degenerating neurons

38 Fluoro-Jade C (FJ-C) labeling was used to verify AMPH-in

39 were examined via hematoxylin and eosin and Fluoro-Jade C (identifying dying neurons) staining in CA

40 ved after neurotoxic insult by staining with Fluoro-Jade C dye.

41 Specifically, Fluoro-Jade C exhibited the greatest signal to backgroun

42 ve also combined laminin immunolabeling with Fluoro-Jade C labeling to evaluate the spatio-temporal a

43 rotein levels (dopamine terminal marker) and Fluoro-Jade C positive cells (degenerating cells).

44 Mice resuscitated with PNPH had fewer Fluoro-Jade C positive neurons in CA1 vs. Hextend and la

45 ssessments, including neurological deficits, Fluoro-Jade C staining, brain edema, Evans blue extravas

46 umber of degenerating neurons observed after Fluoro-Jade C staining.

47 o-infused OCT and NMDA and measured 3-NT and Fluoro-Jade C staining.

48 as "poor survivors" by both histological and Fluoro-Jade C staining.

49 otor behavioral parameters and staining with Fluoro-Jade C, a dye that specifically recognizes apopto

50 lished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astro

51 Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fl

52 Fluoro-Jade can be combined with other fluorescent metho

53 an ideal suppressed silver preparation, the Fluoro-Jade dyes were developed.

54 iration, 3-NPA, were the same for all of the Fluoro-Jade dyes.

55 solectin B4-labeled phagocytic microglia and Fluoro-Jade (F-J)-labeled neurons in the somatosensory p

56 The Fluoro-Jade (FJ) stain reliably identifies degenerating

57 Fluoro-Jade (FJ) staining revealed neuronal degeneration

58 their brains were sectioned and stained with Fluoro-Jade (FJ), a dye that stains injured neurons.

59 we used a wide variety of indicators such as Fluoro-Jade (FJ), a slightly modified fluorescein sold a

60 Like Fluoro-Jade, Fluoro-Jade B is compatible with a number o

61 By using Fluoro-jade histochemistry to mark neurodegeneration and

62 Compared to conventional methodologies, Fluoro-Jade is a more sensitive and definitive marker of

63 Fluoro-Jade is an anionic fluorochrome capable of select

64 Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful

65 The regional distribution of Fluoro-Jade-labeled neurons corresponded to a similar pa

66 Two techniques (Fluoro-Jade labeling and argyrophylia) showed that neuro

67 ebrain was characterized by B4 isolectin and Fluoro-Jade labeling techniques after 4 doses of 15 mg/k

68 eath in these regions, as assessed using the Fluoro-Jade method.

69 Fluoro-Jade positive degenerating neurons were observed

70 t populations, we isolated total RNA from 25 Fluoro-Jade-positive and 25 Newport Green-positive pyram

71 localized with glutamate, reduced numbers of Fluoro-Jade-positive and Newport Green-positive neurons

72 Fluoro-Jade-positive neurons were counted in hippocampal

73 The histochemical application of Fluoro-Jade results in a simple, sensitive and reliable

74 Complementary Fluoro-Jade, silver impregnation methods and TUNEL were

75 taining with a newly developed fluorochrome (Fluoro-Jade) specific for degenerating neurons, was used

76 stained with a newly developed fluorochrome (Fluoro-Jade) specific for degenerating neurons.

77 ifically, there was an eightfold increase in Fluoro-jade staining in the p50 knockout group vs. the n

78 mined by hematoxylin and eosin as well as by Fluoro-Jade staining.

79 not show any neurotoxicity as visualized by Fluoro-Jade staining.

80 s rapid, simple and reliable as the original Fluoro-Jade technique.

81 dence of degenerating neurons as detected by Fluoro-Jade was found in any experimental group.