ライフサイエンスコーパス: G418

1 G418 binds to both the human and E. coli rRNA A sites wi

2 G418 failed to activate PI-PLC when ConA was present.

3 G418 increased read-through in selenium-replete cells as

4 G418 selected cardiomyocytes were tested for their abili

5 G418 treatment increased immunodetectable endogenous or

6 G418, which appears to act on the enzyme.substrate compl

7 G418-resistant A9 cell transformants expressing Ad2 rece

8 G418-resistant cells selected following transfection wit

9 G418-resistant colonies were selected and further select

10 G418-resistant ES cell clones are induced to differentia

11 ning the TRES, gave titers of 10(4) to 10(5) G418-resistant CFU/ml on murine NIH 3T3 cells.

12 1q25 deleted region, were retrofitted with a G418 resistance marker and transfected into murine A9 fi

13 2, and several clones were established after G418 selection.

14 Although G418 exhibits the strongest activity, it is very cytotox

15 AR1 are hypersensitive to the aminoglycoside G418.

16 Se metabolism, we tested the aminoglycosides G418 and gentamicin in hepatoma cell lines (HepG2, Hep3B

17 C-SIN amphotropic G418-resistant virus particles were generated with a tit

18 nsion and selection with the neomycin analog G418, cells derived from transduced progenitor cells wer

19 ants were selected using the neomycin analog G418.

20 neo gene are selected using the neo analogue G418.

21 ation to resistance to the neomycin analogue G418 were measured, and the nature of the mutations was

22 d-ornithine (d-Orn), a substrate analog, and G418 (Geneticin), a weak non-competitive inhibitor, was

23 After transfection, blasticidin and G418-resistant parasites tested positive for plasmid rep

24 MCF viruses encoding beta-galactosidase and G418 resistance.

25 ificantly lower than those of gentamicin and G418.

26 ide antibiotics kanamycin A, gentamycin, and G418 stimulated PI-PLC cleavage of the GPI anchor of var

27 ropriate selection (vincristine for MDR1 and G418 for neor).

28 d cell sorting, fluorescence microscopy, and G418 selection.

29 of the 2-DOS aminoglycosides paromomycin and G418 (geneticin) are compared, using both human and Esch

30 Paromomycin and G418 differ with respect to their specificities of actio

31 esis inhibitors hygromycin B, puromycin, and G418.

32 duce TXA(2) was created by gene transfer and G418 selection.

33 The aminoglycoside antibiotic G418 is a highly specific and potent inhibitor of STIM2-

34 transferase, for selection by the antibiotic G418, are expressed as a fusion, beta Geo.

35 are put under selection with the antibiotic G418, cell lines can be obtained at high frequency that

36 lected for in the presence of the antibiotic G418.

37 istance in mammalian cells to the antibiotic G418.

38 s were obtained by screening with antibiotic G418.

39 achieved by electroporation and antibiotic (G418) selection.

40 PI-PLC was activated by G418 even in the presence of the inhibitor VP-616L.

41 omogeneous FGSC cultures were established by G418 selection and continued to express ziwi for more th

42 signal sequence into HepG2 cells followed by G418 selection.

43 Resistant replicon RNAs were generated by G418 selection in the presence of SCH6 in a dose-depende

44 ed activity of protein variants generated by G418-mediated suppression.

45 ides a potential mechanism for inhibition by G418 and suggests that allosteric inhibition from this s

46 silencing of STIM2 by siRNA or inhibition by G418 suppresses store-operated Ca(2+) entry and agonist-

47 Clones were obtained by G418 selection and screened for IRF-1 mRNA expression by

48 ch integration had occurred were selected by G418 resistance.

49 hEST2 and individual clones were selected by G418.

50 ailability, and was completely suppressed by G418 under Se-poor conditions.

51 ty of the HK2 Bogota reporter was tracked by G418 resistance.

52 two synthetic CB1 and CB2 peptides, CB1I397-G418 and CB2I298-K319, respectively, in membrane mimetic

53 40 nt, alpha2A/D-AR) and a vector conferring G418 resistance.

54 a3 gene, and a Pgk-neo cassette that confers G418 resistance to mammalian cells.

55 he neo(R) expression cassette, which confers G418 resistance, was used to select for illegitimate int

56 After culture in media containing G418 for 3 weeks, approximately 0.1% of cells previously

57 of colonies that were resistant to the drug G418, a neomycin analog.

58 ed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above

59 wo translational readthrough inducing drugs (G418 & PTC124) to investigate potential restoration of e

60 e upstream and downstream CFTR codons during G418-mediated suppression.

61 ved by culturing heterozygotes with elevated G418.

62 motifs were replaced by a cassette encoding G418 resistance and beta-galactosidase, under the contro

63 in unselected cell populations and following G418 selection to obtain uniformly transduced cell popul

64 ent from those recently identified following G418-mediated suppression of the CFTR G542X UGA mutation

65 Electron density for G418 was observed at the boundary between the two domain

66 eading frame were recovered by selecting for G418-resistant colonies.

67 ysis of the HCV replicon RNAs recovered from G418-resistant Huh7 cells revealed several interesting f

68 Geneticin (G418) was then identified as a potent inducer of the Del

69 ide antibiotic read-through agent geneticin (G418) across a diverse range of in vitro reporter assays

70 now available that gentamicin and Geneticin (G418) can induce the mammalian ribosome to suppress dise

71 doxycycline, chloramphenicol, and Geneticin (G418) interfered with insertion of selenocysteine (Sec),

72 itors in culture, blasticidin and geneticin (G418), were selected for further study.

73 fied minimal media that contained geneticin (G418).

74 some in complex with paromomycin, geneticin (G418), gentamicin, and TC007, solved at 3.3- to 3.7-A re

75 the Leishmania ribosomal A site: Geneticin (G418), a potent aminoglycoside for the treatment of leis

76 pterin, and 1.6 x 10(-5) M thymidine (HAT) + G418; and (c) the presence in genomic DNA of genes for b

77 Transduced cells were cloned in G418 and expanded for analysis of IGF-1 transgene expres

78 Culture in G418 resulted in elimination of the mesenchymal cells, l

79 red, allowing for selection of revertants in G418.

80 The feeder cell line was selected in G418 and engineered to express zebrafish leukemia inhibi

81 HBV genome and the neo gene and selected in G418 to generate stable cell lines.

82 The transduced cells were selected in G418, an antibiotic toxic to normal mammalian cells.

83 ading frame of the neo gene were selected in G418, and mutation rates were determined by fluctuation

84 r, and stable transformants were selected in G418.

85 ficantly impaired detection (by selection in G418) of genomic events associated with reactivation of

86 ontains a neo gene for positive selection in G418, an I-SceI endonuclease recognition sequence for in

87 After selection in G418, more than 90% of the cells contained the TK gene a

88 ls could not be propagated upon selection in G418-containing media, suggesting nuclear accumulation o

89 Upon selection in the presence of increasing G418 concentrations, transduced NK cells were able to pr

90 se resistance to nourseothricin or kanamycin/G418 to select for propagation in prototrophic hosts.

91 ne was selected in vitro using 800 microg/ml G418.

92 body recognition are E2 residues L413, N415, G418, and W420.

93 puromycin-resistant 293 cells) and MDBK-Neo (G418-resistant MDBK cells) cell lines for total cellular

94 transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear tr

95 ontaining the enhanced GFP and the neomycin (G418) resistance gene.

96 No G418-resistant colonies were present in cultures infecte

97 No G418-resistant progenitor cell colonies were present in

98 neomycin, an aminoglycoside antibiotic (not G418-neomicin), resulted in reduced KSHV latent gene exp

99 ned in either the presence or the absence of G418 sulfate and were passaged weekly.

100 after 6 months in culture in the absence of G418 sulfate, approximately 90% of the cells in each clo

101 were confirmed by Southern blot analysis of G418- and 6-thioguanine (6TG)-resistant clones.

102 By contrast, the binding of G418 induces the destacking of base 1492 when either rRN

103 that are amplified in high concentrations of G418.

104 Se content of G418-induced SELENOP was dependent on Se availability, a

105 We studied the effects of G418, an aminoglycoside, on cellular glutathione peroxid

106 50 micromol/L, induced > or = 90% killing of G418-selected cells without affecting nontransduced cell

107 pe HCV-N drastically decreased the number of G418-resistant colonies obtained following transfection

108 AGS1/RASD1 markedly diminished the number of G418-resistant colonies, whereas the Ras subgroup member

109 colony assay did not increase the number of G418-resistant colonies.

110 crease by orders of magnitude the numbers of G418-resistant colonies selected following transfection

111 Significantly higher numbers of G418-resistant colonies were produced in cultures initia

112 Our analysis detected a population of G418(S)/neo(+) Tf1 integration events that would have be

113 sciatic nerve were grown in the presence of G418, and within 8-9 d exposure to antibiotic, 99% of al

114 bencephalon were cultured in the presence of G418, surviving cells differentiated into OLs, indicatin

115 Selection was in the presence of G418.

116 verall, neither the frequency of recovery of G418-resistant colonies nor the sizes of NHEJ-associated

117 iterative transfections and the selection of G418-resistant cell clones, form two groups associating

118 ) selectable marker resulted in selection of G418-resistant cell lines that effectively colonize reci

119 lar in vitro and ex vivo activity to that of G418, while its cell toxicity is significantly lower tha

120 Of 11 clones selected by virtue of G418 resistance and positive EpoR expression, 5 clones s

121 ility to confer resistance to blasticidin or G418, respectively.

122 presence of doxycycline, chloramphenicol, or G418, the Sec-containing form of TR1 decreased, whereas

123 '-carbon of gentamicin X2 (GenX2) to produce G418 during the biosynthesis of gentamicins.

124 pendent Sec incorporation is able to repress G418-induced UGA readthrough as well as eRF1-induced sti

125 The resulting G418-sensitive homozygous mutants should allow mutagenes

126 Since G418 stimulates a purified enzyme that is not involved i

127 suppressor (Glu) are expressed from a single G418 selectable vector, while a gene engineered to conta

128 ment of patient cell lines demonstrated that G418 increases SMN levels and is a potential lead compou

129 These observations indicate that G418 is an allosteric activator of Bacillus cereus PI-PL

130 mass spectrometry experiments indicated that G418 caused a substitution of l-arginine for selenocyste

131 These data show that G418 can affect the biosynthesis of this key antioxidant

132 The G418-resistant (G418R)-LNCaP clones that expressed a hig

133 Further RNA analysis from the G418(S)/neo(+) clones revealed 50% of clones expressing

134 in the expression of receptor protein in the G418-resistant transfectants (nt 74, 100%; nt 143, 80%;

135 was highly effective (99.4 to 99.9%) in the G418-selected cultures.

136 hich induced resistance of infected cells to G418.

137 licons were selected by constant exposure to G418 sulfate.

138 genes neo and ble, conferring resistance to G418 and phleomycin, respectively, 1 kb downstream of "s

139 tein (GFP) and neo (conferring resistance to G418).

140 y, transformation by v-src and resistance to G418, following infection of cells.

141 integrants can be selected by resistance to G418.

142 Cellular clones resistant to G418 antibiotic were selected and expanded for further a

143 NOP levels increased strongly in response to G418, whereas expression of the glutathione peroxidases

144 also capable of replication and gave rise to G418-resistant cell clones following transfection of Huh

145 ere differentiated in vitro and subjected to G418 selection.

146 lational read-through inducing drugs (TRIDs) G418 and PTC124 (Ataluren), we were able to restore up t

147 quence, cells were subsequently placed under G418 selection and conversion confirmed at the genetic l

148 Using G418 selection, we routinely obtained vector particles p

149 ently subjected to positive selection (using G418 to select for neomycin-resistance) and negative sel

150 leukin-6 (IL-6), and IL-3, the cultures were G418-selected, and then challenged with HIV-1(JR-FL) and

151 Luc activity of PTC124 but found that, while G418 exhibits varying activity in every read-through ass

152 Furthermore, treatment of SMA mice with G418 increased both SMN protein and mouse motor function

153 to C57BL/6 ES cells and clones selected with G418 followed by injection into Balb/c blastocysts.

154 ell clones expressing RFP were selected with G418 sulfate and individual clones were isolated.

155 s, and resistant colonies were selected with G418.

156 CREF cells were generated by selection with G418 and compared with respect to transformed properties

157 ression construct followed by selection with G418 for 10-14 days.

158 After selection with G418, Bax, Bcl-2 and pCI-neo stably transfected cells we

159 rovirus infection followed by selection with G418.

160 ectants were obtained through selection with G418.

161 ection of LZRN-transduced cells in situ with G418.

162 es, initiated from testes, were treated with G418 to eliminate the somatic cells and select for the p

163 sion PC-3 clones were selected in vitro with G418, which were then combined and injected s.c. in nude