ライフサイエンスコーパス: GABP

1 GABP (also known as nuclear respiratory factor 2) is a n

2 GABP alpha and GABP beta 1 bind the insulin-response ele

3 GABP alpha and PU.1 competed with each other for binding

4 GABP alpha bound to the mNE ets site and, in turn, recru

5 GABP alpha immunoprecipitated from insulin-treated, 32P-

6 GABP also activates the CD18 promoter in Schneider cells

7 GABP also directly regulates Foxo3 and Pten and hence su

8 GABP binds DNA containing a single PEA3/Ets-binding site

9 GABP cooperated with c-Myb and C/EBP alpha to activate t

10 GABP could bind to the regulatory regions of Pax5 and Cd

11 GABP increased the activity of the mNE promoter sevenfol

12 GABP is a heteromeric transcription factor complex that

13 GABP is a heteromeric transcription factor complex which

14 GABP is an ets transcription factor that regulates genes

15 GABP is composed of two subunits, the Ets-related GABP-a

16 GABP is composed of two subunits; the GABPalpha subunit

17 GABP may be regulated by MAP kinase phosphorylation.

18 GABP thus directly links TERT promoter mutations to aber

19 GABP, also known as nuclear respiratory factor 2, regula

20 GABP, of which GABPA is a component, is known to regulat

21 GABP, which is present in human fibroblast nuclear extra

22 amily consensus sequences, which bound PU.1, GABP, and an Ets factor present in myeloid cell extracts

23 confirm the functional relevance of the -119 GABP-binding site in vivo.

24 urprisingly, neither coactivator binds NRF-2(GABP), a multisubunit transcriptional activator associat

25 spiratory factor 2/GA binding protein (NRF-2/GABP), and ying-yang protein 1 (YY1).

26 urther showed that pro-IL-16 is located in a GABP transcriptional complex bound to the Skp2 promoter.

27 epsilon subunit gene, and that it requires a GABP binding site within this region.

28 These systematic analyses revealed a GABP-controlled gene regulatory module that programs mul

29 -specific MuSK gene expression by activating GABP(alphabeta) transcription factors in endplate-associ

30 In addition, GABP is critically required for antigen-stimulated T-cel

31 mobility shift assay with antibodies against GABP and AP-1, respectively.

32 ial cells with small interfering RNA against GABP and SP1 resulted in a significant (approximately 50

33 A binding of GH4NE containing both Ets-1 and GABP and HeLa nuclear extracts devoid of Ets-1 but conta

34 transcription is regulated by both PU.1 and GABP in developing B cells.

35 The ets factors, PU.1 and GABP, bind to three ets sites in the CD18 promoter, whic

36 arin-Sepharose, we have shown that Ets-1 and GABP, which are MAP kinase substrates, co-purify with co

37 subset of genes controlled by PGC-1alpha and GABP is dysregulated in Duchenne muscular dystrophy (DMD

38 rate here that GABP DNA binding activity and GABP-dependent gene expression in 3T3 cells are inhibite

39 GABP alpha and GABP beta 1 bind the insulin-response element of the pro

40 Expression of GABP alpha and GABP beta 1 squelches insulin-increased prolactin gene e

41 ha, which mediates specific DNA binding, and GABP-beta, which forms heterodimers and heterotetramers

42 t consists of GABP alpha, an ets factor, and GABP beta, a Notch-related protein.

43 the cellular transcription factors LZIP and GABP and also plays an essential role in cell proliferat

44 data for the transcription factors NRSF and GABP.

45 Cotransfection of Sp- and GABP-deficient Drosophila SL-2 cells with the HPR1 promo

46 Co-transfection of Sp1 and GABP activates CD18 more than the sum of their individua

47 element of the prolactin promoter, and anti-GABP alpha and anti-GABP beta 1 antibodies supershift a

48 actin promoter, and anti-GABP alpha and anti-GABP beta 1 antibodies supershift a species seen with nu

49 As GABP is widely expressed, a strong TATA box thus allevia

50 al genes that were previously reported to be GABP targets.

51 Both GABP and PU.1 bound Ets sites in the Lbr promoter in vit

52 tially binds Ets-1, while the BTE binds both GABP and Ets-1.

53 analysis of Fas promoter revealed that both GABP- and AP-1-binding sites were required for initiatin

54 of C/EBPepsilon, and activities supported by GABP were greatly enhanced by either C/EBPepsilon or PU.

55 transactivation potential also impair the C1-GABP interaction, indicating that the C1 factor function

56 In committed B lineage cells, GABP can promote Il7r transcription in the absence of PU

57 In T cells, GABP has been demonstrated to regulate the gene expressi

58 cond transcription factor from myeloid cells-GABP-binds to the mNE ets site but strongly activates th

59 lear extracts devoid of Ets-1 but containing GABP, we were able to show that the EBS-RRE preferential

60 rimarily for the transcription factors CTCF, GABP, GATA2, E2F, STAT, and YY1.

61 dies show that endogenous, pituitary-derived GABP and Ets-1 bind to the BTE, whereas Ets-1 preferenti

62 cluding Bcl-2, Bcl-X(L), and Mcl-1 as direct GABP target genes, underlying its pivotal role in HSC su

63 ses determined the association of endogenous GABP as well as Sp3 proteins with the -727/-476 region o

64 Thus, our results establish GABP as both a transcriptional activator factor and as a

65 ddition to regulating IL-7Ralpha expression, GABP is critically required for TCR rearrangements and h

66 s to activate TERT, probably by facilitating GABP heterotetramer binding.

67 ntified the Ets-related transcription factor GABP in nuclear extracts from GH cells.

68 ED1 and the Ets-related transcription factor GABP.

69 and GA-binding protein transcription factor (GABP-alpha) to their respective activator protein 1 (AP1

70 sults suggest that the transcription factors GABP and AP-1 play a critical role in the induction of F

71 Sp1 activated the distal enhancer 5-fold, GABP 3-fold, and the combination 8-fold in Schneider cel

72 PU.1 binding site and for PU.1, but not for GABP.

73 GABPalpha cDNA sequence including sites for GABP (-86, -104, -169, -257, and -994), YY1 (-57), Sp1 (

74 d within a 400 bp sequence and contains four GABP binding sites, a Sp1/3 binding site and an YY1 bind

75 Furthermore, GABP activates transcription of DNA methyltransferases a

76 leading algorithms using H3K27me3, H3K36me3, GABP, ESR1 and FOXA ChIP-seq datasets.

77 ome-wide GABP-binding site analyses identify GABP direct targets encoding proteins involved in cellul

78 termine the roles of individual cysteines in GABP redox regulation, we generated a series of serine s

79 tein complex--an enhanceosome--that includes GABP, other transcription factors, and coactivators, dyn

80 sulfhydryl-alkylating agents also inhibited GABP DNA binding activity.

81 and by thioredoxin; however, GSSG inhibited GABP DNA binding activity.

82 Instead, GABP provided transcriptional activation through the Lbr

83 Treatment of GABP-alpha, but not GABP-beta1, with sulfhydryl-alkylating agents also inhib

84 ion-associated factors, including SRF, NRSF, GABP, Stat3 and p300 in different developmental contexts

85 ases might directly regulate the activity of GABP, we studied MAP kinase-catalyzed and NRG-1-induced

86 ts, which were proportional to the amount of GABP added, required both the GABP alpha subunit and eit

87 cell activation increased the DNA binding of GABP and AP-1 to this enhancer site.

88 Binding of GABP to the GAA/CF6 bi-directional promoter provides the

89 The binding of GABP to the rpS16 initiation region does not significant

90 1 factor functions as a novel coactivator of GABP-mediated transcription.

91 ranscription factor complex that consists of GABP alpha, an ets factor, and GABP beta, a Notch-relate

92 Finally, small interfering RNA depletion of GABP in GH3 cells results in the loss of prolactin prote

93 kinase D2 (PRKD2) as a potential effector of GABP in HSCs.

94 Expression of GABP alpha and GABP beta 1 squelches insulin-increased p

95 Forced expression of GABP significantly enhanced R-ras mRNA expression level

96 ha expression and confirms the importance of GABP in the coordinate expression of respiratory chain c

97 d cells shows substantial phosphorylation of GABP alpha.

98 There was no increase in phosphorylation of GABP beta in response to insulin.

99 sing the possibility that phosphorylation of GABP by MAP kinases induces transcription of AChR genes.

100 Allelic recruitment of GABP is consistently observed across four cancer types,

101 The regulation of GABP (nuclear respiratory factor 2) DNA binding activity

102 To study the role of GABP at neuromuscular synapses, we conditionally inactiv

103 To examine the role of GABP in myeloid differentiation, we generated mice in wh

104 The specificity of GABP and AP-1 binding was demonstrated by competition el

105 1 (54 kDa) and/or the DNA binding subunit of GABP, GABPalpha (57 kDa).

106 ion of GABPalpha, the DNA-binding subunit of GABP, leads to early embryonic lethality, preventing ana

107 at GABPalpha, the ets DNA-binding subunit of GABP, physically interacts with p300 in myeloid cells.

108 ncy of GABPalpha, the DNA-binding subunit of GABP, resulted in profoundly defective B cell developmen

109 Although expression of rb, a target of GABP, is elevated in muscle tissue deficient in GABPalph

110 Treatment of GABP-alpha, but not GABP-beta1, with sulfhydryl-alkylati

111 expression vector encoding the Sp1, Sp3, or GABP gene induced luciferase gene expression.

112 ites functioned cooperatively with the other GABP binding sites and with the Sp1/3 and YY1 sites to p

113 The other GABP sites and the Sp1/3 and YY1 binding sites were func

114 ed from control cells does not phosphorylate GABP alpha while MAP kinase immunoprecipitated from insu

115 Potential GABP-binding sites are present in the promoters of numer

116 ription factors PU.1 and GA-binding protein (GABP) activate the Il7r promoter by interacting with a h

117 lation of PGC-1alpha and GA-binding protein (GABP) allows recruitment of PGC-1alpha to the GABP compl

118 f this complex to be the GA-binding protein (GABP) alpha.

119 erized the heterodimeric GA-binding protein (GABP) alphabeta complex and focused specifically on the

120 ontains highly conserved GA-binding protein (GABP) and YY1 binding sites, conferred high transcriptio

121 we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regula

122 GA binding protein (GABP) consists of GABPalpha and GABPbeta subunits.

123 ed by immunodepletion of GA-binding protein (GABP) from FM3A cell nuclear extracts.

124 ain transcription factor GA-binding protein (GABP) has been implicated to mediate synapse-specific ge

125 Although Ets-1 and GA binding protein (GABP) have been implicated in the Ras and insulin respon

126 studied the assembly of GA-binding protein (GABP) in solution and established the role of DNA in the

127 ily transcription factor GA-binding protein (GABP) in T cells impairs T-cell homeostasis.

128 ts) transcription factor GA binding protein (GABP) is a tetrameric transcription factor complex that

129 GA-binding protein (GABP) is a transcriptional regulator composed of two str

130 The GA-binding protein (GABP) is a ubiquitous heteromeric transcription factor i

131 The GA-repeat binding protein (GABP) is a ubiquitous transcription factor involved in t

132 GA binding protein (GABP) is a ubiquitously expressed Ets family transcripti

133 GA binding protein (GABP) is a ubiquitously expressed Ets-family transcripti

134 The transcription factor GA-binding protein (GABP) is composed of two subunits, GABPalpha and GABPbet

135 GA-binding protein (GABP) is the only Ets family transcription factor that f

136 nstructs encoding either GA-binding protein (GABP) or PU.1 inhibited Sp1-mediated promoter activation

137 2 and -682) containing a GA-binding protein (GABP) site and a low affinity activating protein-1 (AP-1

138 itment of the multimeric GA-binding protein (GABP) transcription factor specifically to the mutant pr

139 and to the ERE sites by GA-binding protein (GABP) was confirmed by electrophoretic mobility shift as

140 The GA-binding protein (GABP), a heterodimeric transcription factor with widespr

141 ents the Pd from binding GA-binding protein (GABP), a transcription factor essential for Pd transcrip

142 ts transcription factor, GA-binding protein (GABP), and Sp1 were required for full RA responsiveness

143 n factor family protein, GA-binding protein (GABP), binds to the R-ras-derived sequence.

144 The transcription factor GA binding protein (GABP), consisting of DNA-binding subunit GABPalpha and t

145 TS transcription factor, GA-binding protein (GABP), with the relatively lineage-restricted E-twenty-s

146 Moreover, a GA-binding protein (GABP)-binding motif at -119 was necessary for mediating

147 factors such as Sp1 and GA-binding protein (GABP).

148 ric transcription factor GA-binding protein (GABP).

149 ember of the ETS family, GA binding protein (GABP).

150 mplexes, which contained GA-binding protein (GABP).

151 sites that are bound by GA-binding protein (GABP).

152 lly and functionally, as GA-binding protein (GABP)alpha/GABPbeta.

153 DNA binding of recombinant GABP-alpha was activated by chemical reduction (dithioth

154 to the mNE ets site and, in turn, recruited GABP beta to form a transcriptionally active complex.

155 have a dominant negative role in regulating GABP target genes.

156 is composed of two subunits, the Ets-related GABP-alpha, which mediates specific DNA binding, and GAB

157 In summary, serum-responsive GABP binding to Ets-binding sites activates the KIS prom

158 Mutations of the rpS16 GABP-binding sites that abolish binding increased rpS16

159 the more ubiquitously expressed factors Sp1, GABP alpha/beta, and NF2d9 are responsible for governing

160 tes for the human transcription factors SRF, GABP and NRSF at an average resolution of about 20 base

161 in vitro, whereas mutations that strengthen GABP binding caused a reduction in promoter activity.

162 The tetrameric GABP complex includes GABPalpha, which binds DNA via its

163 GH cells was phosphorylated 3-fold more than GABP alpha from control cells.

164 These findings demonstrate that GABP, PU.1, and C/EBPepsilon cooperate to control transc

165 We previously demonstrated that GABP is subject to redox regulation in vitro and in vivo

166 A-induced enhanceosome and demonstrates that GABP and p300 are essential components of CD18 RA respon

167 We demonstrate here that GABP binds to the rpS16 initiation region, and in so doi

168 We demonstrate here that GABP DNA binding activity and GABP-dependent gene expres

169 er studies, these new findings indicate that GABP can have a dual role as repressor or activator of r

170 These data indicate that GABP is dispensable for synapse-specific transcription a

171 These findings show that GABP has a nonredundant role in the control of T-cell ho

172 Our results suggest that GABP DNA binding activity is redox-regulated in vivo, po

173 These studies suggest that GABP mediates insulin-increased transcription of the pro

174 The GABP complex, consisting of DNA binding GABPalpha subuni

175 tric analysis of the interaction between the GABP subunits determined an association constant (K(A))

176 the amount of GABP added, required both the GABP alpha subunit and either a beta1 or beta2 subunit.

177 the Fas enhancer, and mutation of either the GABP or AP-1 binding site severely reduced transcription

178 n such genes, e.g. the mouse rpL30 gene, the GABP-binding sites are located 40-80 base pairs upstream

179 l-related kinase-dependent activation of the GABP(alpha/beta) transcription factor complex.

180 uction and by GSSG-mediated oxidation of the GABP-alpha subunit.

181 a provide strong support for the role of the GABP-binding motif in mediating Robo4 expression in the

182 cally ascribed to perturbed integrity of the GABP-controlled gene regulatory module in HSCs.

183 In addition, mutations that reduce the GABP transactivation potential also impair the C1-GABP i

184 In other studies, the GABP mutation was introduced into the endogenous mouse R

185 R-ras promoter sequence and suggest that the GABP may be critical for transcription of R-ras and for

186 ABP) allows recruitment of PGC-1alpha to the GABP complex and enhances transcription of a broad neuro

187 Robo4-LacZ transgenic cassette in which the GABP site was mutated.

188 Among these GABP-associated genes, knockdown of GABPalpha expression

189 Thus, GABP binds to the crucial mNE promoter ets site and powe

190 Thus, GABP exists in solution as the heterodimer previously sh

191 Thus, GABP is a key regulator of B cell development, maturatio

192 Thus, GABP is essential for the regulation of IL-7Ralpha expre

193 Thus, GABP is regulated through at least two redox-sensitive a

194 Thus, GABP is required for HSC cell cycle entry and CML develo

195 sions were significantly smaller compared to GABP+/+ mice.

196 Whereas GABP operates as an essential upstream activator, PU.1 a

197 Transcriptome and genome-wide GABP-binding site analyses identify GABP direct targets

198 ollectively suggest that Sp1 cooperates with GABP to regulate HPR1 promoter activity.

199 Supplementation of cell-free extracts with GABP inhibits transcription on rpS16 templates while con

200 , a direct interaction of the C1 factor with GABP is demonstrated, defining the C1 factor as the crit

201 ctivator p300/CBP physically interacted with GABP in vivo, and p300 increased the responsiveness of t