ライフサイエンスコーパス: GDP fucose

1 th proteins were able to salvage l-fucose to GDP-fucose.

2 to synthesize the donor for fucose addition, GDP-fucose.

3 e one to rescue designer mice unable to make GDP-Fucose.

4 residue that lies near the binding site for GDP-fucose.

5 -5'-[alpha-(32)P] triphosphate, an analog of GDP-fucose.

6 nerate GDP-Arap, while synthesizing abundant GDP-fucose.

7 involved in the conversion of GDP-mannose to GDP-fucose.

8 De novo synthesis of guanosine diphosphate (GDP)-fucose, a substrate for fucosylglycans, requires se

9 De novo synthesis of guanosine diphosphate (GDP)-fucose, a substrate for fucosylglycans, requires se

10 CB CD34(+) cells with guanosine diphosphate (GDP) fucose and exogenous alpha1-3 fucosyltransferase VI

11 charide linked to C-6 of a fucose residue in GDP-fucose and a milk fucosyltransferase.

12 Kinetic analysis indicates that GDP-fucose and xyloglucan associate with the enzyme in a

13 he Km values for factor VII EGF-1 domain and GDP-fucose are 15 and 6 microM, respectively.

14 Given that bulkier analogs like GDP-fucose are also accommodated at this site, it seems

15 for site-specific fucosylation map near the GDP-fucose-binding site.

16 Injection of GDP-fucose but not fucose into C. elegans intestinal cel

17 up designed to mimic the transition state of GDP-fucose complexed with Mn(II) in fucosyltransferase r

18 ogenous alpha1-3-fucosyltransferase FTVI and GDP-fucose created many new epitopes for anti-sLe(x) mAb

19 on produce the same biochemical phenotype of GDP-fucose deficiency.

20 These results establish a requirement for GDP-fucose for L. major viability and predict the existe

21 No significant change occurred in the Km for GDP-fucose for this protein when compared with FucT V.

22 alyzes the first step in the biosynthesis of GDP-fucose from GDP-mannose.

23 Previous analyses showed the presence of GDP-fucose (GDP-Fuc), the precursor for all fucosylation

24 A GDP-fucose:GM1 alpha1-->2 fucosyltransferase (FucT) is i

25 one are sufficient to convert GDP mannose to GDP fucose in vitro.

26 e, UDP-N-acetylglucosamine, GDP-mannose, and GDP-fucose in Plasmodium falciparum intraerythrocytic li

27 those observed in mice unable to synthesize GDP-fucose, indicating the existence of another mechanis

28 syltransferase that incorporates fucose from GDP-fucose into xyloglucan, adding it preferentially to

29 Starting from L-fucose, GDP-fucose is constructed by one bifunctional enzyme L-f

30 at are active in vitro, indicating that most GDP-fucose is formed by a de novo pathway that involves

31 To test whether GDP-fucose itself was essential for Leishmania viability

32 lbeta(1-->4)GlcNAc unit, and a corresponding GDP-fucose:N-acetylglucosaminyl alpha(1,3) fucosyltransf

33 a neutral pH optimum, and exhibited a Km for GDP-fucose of 0.34 microM, a Km for pNP-LNB of 0.6 mM, a

34 e transporter that competes with Slc35c1 for GDP-fucose, or a factor that otherwise enhances the fuco

35 ion of the transporters for CMP-sialic acid, GDP-fucose, or both unexpectedly resulted in accumulatio

36 A GDP-fucose:polypeptide fucosyltransferase was purified 5

37 Thus, the GDP-fucose precursor is essential in a wide variety of o

38 No change in GDP-fucose-protectable pyridoxal-P/NaBH4 inactivation wa

39 ose to epidermal growth factor-like repeats, GDP-fucose protein O-fucosyltransferase (O-FucT-1), was

40 This method exploits l-fucokinase/GDP-fucose pyrophosphorylase (FKP), a bifunctional enzym

41 re deficient in conversion of GDP-mannose to GDP-fucose substantially decreased the levels of secrete

42 n the parasite is not known, the presence of GDP-fucose suggests that the metabolite may be used for

43 a lack of fucosylation consequent to loss of GDP-fucose synthesis contributes to colon carcinogenesis

44 encodes an enzyme in the de novo pathway for GDP-fucose synthesis, exhibit a virtually complete defic

45 ive in N-acetylglucosaminyltransferase V and GDP-fucose synthesis, respectively, demonstrating that a

46 etary fucose to supply a salvage pathway for GDP-fucose synthesis.

47 Escherichia coli GDP-mannose dehydratase and GDP-fucose synthetase (GFS) protein have been cloned and

48 ay enzymes GDP-mannose dehydratase (GMD) and GDP-fucose synthetase (GMER) were expressed ectopically;

49 GDP-fucose synthetase from E. coli is a novel dual funct

50 ined guanidine 5'-diphosphate-beta-l-fucose (GDP-fucose), the universal fucosyl donor, the Le(x) tris

51 nylfucose derivatives that depleted cells of GDP-fucose, the substrate used by fucosyltransferases to

52 (FucT) catalyzes the transfer of fucose from GDP-fucose to asparagine-linked GlcNAc of the N-glycan c

53 Fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylact

54 e FUT1 catalyzes the transfer of fucose from GDP-fucose to terminal galactosyl residues on xyloglucan

55 sphatidylcholine liposomes, it was active in GDP-fucose transport and was specifically photolabeled w

56 by leucine in nac(1) flies, which abolishes GDP-fucose transport in vivo and in vitro.

57 A defect in GDP-fucose transport into the lumen of the Golgi apparat

58 ating the existence of another mechanism for GDP-fucose transport into the secretory pathway.

59 that a conserved serine residue in the Golgi GDP-fucose transporter (GFR) is substituted by leucine i

60 Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleoti

61 A potential candidate for a second GDP-fucose transporter is the related gene Slc35c2.

62 he ER, suggesting that a novel, ER-localized GDP-fucose transporter may exist.

63 Inactivation of the Golgi GDP-fucose transporter Slc35c1 in mouse or human does no

64 ned results suggest that Slc35c2 is either a GDP-fucose transporter that competes with Slc35c1 for GD

65 ed and purified the rat liver Golgi membrane GDP-fucose transporter, a protein with an apparent molec

66 It is caused by mutations in the Golgi GDP-fucose transporter, resulting in a reduction of fuco

67 ed fucosylation via over-expression of a key GDP-Fucose transporter, Slc35c1, in zebrafish.

68 SQV-7 did not transport CMP-sialic acid, GDP-fucose, UDP-N-acetylglucosamine, UDP-glucose, or GDP

69 fragilis 9343, which converts l-fucose into GDP-fucose via a fucose-1-phosphate (Fuc-1-P) intermedia

70 Ac) and 4-fold reduction in its affinity for GDP-fucose when compared with FucT III.