ライフサイエンスコーパス: GDP mannose

1 GDP-mannose hydrolase (GDPMH) catalyzes the hydrolysis o

2 GDP-mannose hydrolase catalyzes the hydrolysis with inve

3 GDP-mannose is required for O-mannosylation of proteins,

4 GDP-mannose mannosyl hydrolase (GDPMH) from Escherichia

5 GDP-mannose mannosyl hydrolase (GDPMH) is an unusual Nud

6 GDP-mannose, dolichyl phosphate and substrate analogs we

7 e demonstrated that the VTC1 locus encodes a GDP-mannose pyrophosphorylase (mannose-1-P guanyltransfe

8 Two enzymes interact: a GDP-mannose dependent (1-->4)-beta-D-mannan synthase and

9 Man9GlcNAc2-P-P-dolichol, or inhibition of a GDP-mannose dependent transferase.

10 des a protein with significant homology to a GDP-mannose 4,6-dehydratase, which catalyzes the first s

11 ose, UDP-galactose, UDP-N-acetylglucosamine, GDP-mannose, and GDP-fucose in Plasmodium falciparum int

12 Orf191 (yffH) is a highly active GDP-mannose pyrophosphatase.

13 de, using Kdo2-lipid IVA as the acceptor and GDP-mannose (or synthetic ADP-mannose) as the donor.

14 s of the pathway) as well as UDP-hexoses and GDP-mannose in hind limb muscles and liver in both trans

15 Although M1P and GDP-mannose were below reliable detection/quantification

16 annose-6-phosphate, mannose-1-phosphate, and GDP-mannose, all showed a 3-10-fold decrease in CDGS cel

17 somerase (PMI), phosphomannomutase (PMM) and GDP-mannose pyrophosphorylase (GMP).

18 The donor substrate was identified as GDP-mannose by isothermal titration calorimetry and was

19 capable of generating GDP-glucose as well as GDP-mannose.

20 of Mn2+, indicating the true substrate to be GDP-mannose rather than its Mn2+ complex.

21 ent of this region of the protein in binding GDP-mannose.

22 lucomannan heteropolymers when supplied both GDP-mannose and GDP-glucose.

23 tile cycling the LLO pathway, exacerbated by GDP-mannose/PMM deficiency.

24 ng to Vrg4-HAp was specifically inhibited by GDP-mannose.

25 s, requires sequential reactions mediated by GDP-mannose 4,6-dehydratase (GMDS) and GDP-4-keto-6-deox

26 s, requires sequential reactions mediated by GDP-mannose 4,6-dehydratase (GMDS) and GDP-4-keto-6-deox

27 Extracts of E. coli do not catalyze GDP-mannose-dependent glycosylation of Kdo2-lipid IVA, b

28 a functional homologue of the S. cerevisiae GDP-mannose transporter.

29 enes encoding the human and Escherichia coli GDP-mannose dehydratase and GDP-fucose synthetase (GFS)

30 Escherichia coli GDP-mannose mannosyl hydrolase (GDPMH), a homodimer, cat

31 two proteins alone are sufficient to convert GDP mannose to GDP fucose in vitro.

32 The sugar donors, GDP-mannose and UDP-GlcNAc, must first be transported fr

33 ded a dissociation constant for the binary E-GDP-mannose complex (K(S)(GDPmann)) of 4.0 +/- 0.5 mM, c

34 l mutagenesis of rfbM and galE; genes encode GDP mannose pyrophosphorylase and galactose epimerase re

35 The genes encoding GDP-mannose pyrophosphorylase from Saccharomyces cerevis

36 rst, the trypanosome de novo pathway enzymes GDP-mannose dehydratase (GMD) and GDP-fucose synthetase

37 of epitope-tagged versions of P. falciparum GDP-mannose 4,6-dehydratase and GDP-L-fucose synthase ex

38 A pathway for AsA biosynthesis that features GDP-mannose and L-galactose has recently been proposed f

39 e active when ADP-mannose is substituted for GDP-mannose.

40 The apparent Km values for GDP-mannose and Kdo2-lipid IVA are 4.3 microM and 7.1 mi

41 tes GDP-mannose-4-keto-6-D-deoxymannose from GDP-mannose, which is then converted by the FX protein (

42 triacylphosphatidylinositol dimannoside from GDP-mannose and triacylphosphatidylinositol monomannosid

43 step in the biosynthesis of GDP-fucose from GDP-mannose.

44 g that the capacity to synthesize l-Gal from GDP-mannose is increased.

45 ticulum and catalyzes mannosyl transfer from GDP-mannose to the hydrophobic long-chain acceptor dolic

46 ted activity transfers mannose in vitro from GDP-mannose to an alpha-1, 3-dimannoside acceptor, formi

47 s involved in alpha-1,6 linked fucosylation, GDP-mannose 4, 6-dehydratase (Gmds) and to a lesser exte

48 This enzyme generates GDP-mannose-4-keto-6-D-deoxymannose from GDP-mannose, wh

49 nzyme displays no activity with ADP-glucose, GDP-mannose, UDP-glucose, or UDP-galactose.

50 inducing pathology due to loss of the Golgi GDP mannose transporter.

51 uggesting that the VRG4 gene encodes a Golgi GDP-mannose transporter.

52 nia major deletion mutants lacking the Golgi GDP-mannose transporter LPG2, which is required for asse

53 The Nudix hydrolase GDP-mannose mannosyl hydrolase (Gmm) contributes to this

54 Here we identify GDP-mannose 4,6-dehydratase (GMD) as a binding partner o

55 c-1) was recently shown to have a defect in GDP-mannose pyrophosphorylase, providing strong evidence

56 diverse set of sugar nucleotides, including GDP-mannose, ADP-mannose, UDP-glucose, and ADP-glucose.

57 roteins and lipids in the Golgi apparatus is GDP-mannose, whose lumenal transport is catalyzed by Vrg

58 lgi of the yeast Saccharomyces cerevisiae is GDP-mannose, whose lumenal transport is mediated by the

59 mannosyl constituents of these components is GDP-mannose, which is synthesized through a series of en

60 vrg4 mutant strains were reduced in luminal GDP-mannose transport activity, but this effect could be

61 orrespondingly, null mutants of the L. major GDP-mannose transporter LPG2 lack PGs and are severely c

62 mutations in guanosine diphosphate mannose (GDP-mannose) pyrophosphorylase B (GMPPB) can result in m

63 The structure of the enzyme-Mg2+-GDP-mannose substrate complex of the less active Y103F m

64 ak dissociation constant for the binary Mn2+-GDP-mannose complex (K1 = 6.5 +/- 1.0 mM) which signific

65 Neither UDP-galactose nor GDP-mannose was active as a sugar donor.

66 The sugar nucleotide GDP-mannose is essential for Trypanosoma brucei.

67 under physiological conditions, about 80% of GDP-mannose synthesis comes from the de novo pathway and

68 e novo pathway, which requires the action of GDP-mannose 4,6-dehydratase (GMD) and GDP-L-fucose synth

69 e have characterized the kinetic behavior of GDP-mannose dehydrogenase in detail.

70 The kinetic behavior of GDP-mannose dehydrogenase suggests that it must be at le

71 o pathway that involves the bioconversion of GDP-mannose.

72 uppressed by increasing the concentration of GDP-mannose in vitro.

73 13 cells that are deficient in conversion of GDP-mannose to GDP-fucose substantially decreased the le

74 hesis proteins involved in the conversion of GDP-mannose to GDP-fucose.

75 n plants, which can bypass the deficiency of GDP-mannose production of the vtc1-1 mutant and possibly

76 Depletion of GDP-mannose pyrophosphorylase had a highly pleiotropic e

77 these two sugars begin with the formation of GDP-mannose from d-mannose 1-phosphate and GTP followed

78 GMPPB catalyzes the formation of GDP-mannose from GTP and mannose-1-phosphate.

79 P. falciparum presents homologues of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthase en

80 the subsequent dehydration and oxidation of GDP-mannose to yield GDP-4-keto-6-deoxymannose.

81 roles of the de novo and salvage pathways of GDP-mannose biosynthesis.

82 (M6P) to mannose-1-phosphate, a precursor of GDP-mannose used to make Glc(3)Man(9)GlcNAc(2)-P-P-dolic

83 Pmm2 deficiency depletes M1P, a precursor of GDP-mannose, and consequently suppresses lipid-linked ol

84 In the presence of GDP-mannose and/or UDP-galactose, membranes of R. legumi

85 nts within the cell and in the production of GDP-mannose, an essential alginate precursor, clearly in

86 g evidence for the recently proposed role of GDP-mannose in AsA biosynthesis.

87 rfbKM genes (necessary for the synthesis of GDP-mannose), the rol gene (regulating O-antigen length)

88 1-P), which is required for the synthesis of GDP-mannose, a substrate for dolichol-linked oligosaccha

89 The transport of GDP-mannose from its site of synthesis in the cytosol in

90 e specifically defective in the transport of GDP-mannose into Golgi vesicles.

91 primary role of Vrg4p is in the transport of GDP-mannose into the Golgi.

92 se, UDP-N-acetylglucosamine, UDP-glucose, or GDP-mannose.

93 protein that is highly conserved among other GDP-mannose transporters but not other types of nucleoti

94 e, by a phosphomannomutase (PMM), to produce GDP-mannose, the primary mannose-donor in mycobacteria.

95 This enzyme provides GDP-mannose, which is used for cell wall carbohydrate bi

96 t and, in particular, the steps that require GDP-mannose in the fungal pathogen C. albicans.

97 for these reactions is the nucleotide sugar, GDP-mannose, whose transport into the Golgi from the cyt

98 ce beta-linked mannan polymers when supplied GDP-mannose.

99 The GDP-mannose biosynthesis pathway is highly conserved in

100 The GDP-mannose-dependent enzyme of R. leguminosarum may rep

101 l glucuronoside was used to characterize the GDP-mannose-dependent mannosyltransferase MgtA from C. g

102 ht, but the activity of other enzymes in the GDP-mannose pathway is little affected.

103 Leishmania major parasites lacking the GDP-mannose transporter, termed Deltalpg2 parasites, fai

104 present the crystal structure of an NST, the GDP-mannose transporter Vrg4, in both the substrate-free

105 Genes encoding all the enzymes of the GDP-mannose pathway have previously been identified, wit

106 UDP-galactofuranose transporter GlfB or the GDP-mannose transporter GmtA leads to the absence of gal

107 We conclude that the GDP-mannose pathway is the only significant source of as

108 hosphate, not glucose 1-phosphate; therefore GDP-mannose (guanosine 5'-diphosphomannose) arose predom

109 ies a key position on the de novo pathway to GDP-mannose from glucose, just before intersection with

110 Golgi vesicles from both cells translocated GDP-mannose at comparable velocities, indicating that th

111 The purified enzyme can use GDP-mannose or, to a lesser extent, ADP-mannose (both of

112 from fenugreek endosperm preferentially used GDP-mannose as the substrate for the backbone synthesis.

113 A mannosyltransferase that uses GDP-mannose and the conserved precursor Kdo2-[4'-32P]lip

114 g an N-terminal His(6) tag was assayed using GDP-mannose as the donor and Kdo(2)-[4'-(32)P]lipid IV(A

115 arily involve mannose additions that utilize GDP-mannose as the substrate.

116 selective mannosyl transferase that utilizes GDP-mannose to glycosylate the inner 3-deoxy-D-manno-oct

117 eral viable vrg4 mutants were isolated whose GDP-mannose transport activity was reduced but not oblit

118 sing a coupled assay system that starts with GDP-mannose and Kdo2-[4'-32P]lipid IVA.

119 that amino acids in this region of the yeast GDP-mannose transporter mediate the recognition of or bi

120 aminoimidazole-4-carboxamide ribotide (ZMP), GDP-mannose, and farnesyl pyrophosphate were found to be