ライフサイエンスコーパス: GPAT

1 GPAT activity is known to depend upon channeling of NH(3

2 GPAT and AIRC encode enzymes for steps one and six plus

3 sition, and (3) argue against a role of sn-2-GPATs (Enzyme Commission 2.3.1.198) in membrane/storage

4 Based on these data, we propose a GPAT topography model with two transmembrane domains in

5 nverted by glycerophosphate acyltransferase (GPAT) to 1-acyl-GP.

6 hesis, glycerol-3-phosphate acyltransferase (GPAT) 4 and GPAT8.

7 yl-CoA:glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first step during de novo synthesis

8 Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step in glycer

9 Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and rate-limiting step of gl

10 Glycerol 3-phosphate acyltransferase (GPAT) catalyzes the rate-limiting step of glycerolipid b

11 th the glycerol-3-phosphate acyltransferase (GPAT) family, including a putative catalytic dyad locate

12 eight glycerol-3-phosphate acyltransferase (GPAT) genes that are members of a plant-specific family

13 osomal glycerol-3-phosphate acyltransferase (GPAT) increased 4-fold, liver mass of phospholipid and t

14 Four glycerol-3-phosphate acyltransferase (GPAT) isoforms, each encoded by a separate gene, catalyz

15 ndrial glycerol-3-phosphate acyltransferase (GPAT) was determined using rat liver mitochondria and mu

16 els of glycerol-3-phosphate acyltransferase (GPAT), a mitochondrial enzyme catalyzing the initial ste

17 of sn-glycerol-3-phosphate acyltransferase (GPAT), diacylglycerol acyltransferase (DGAT), and hormon

18 es [sn-glycerol-3-phosphate acyltransferase (GPAT), lysophosphatidic acid acyltransferase (LPAAT), ac

19 of sn-glycerol-3-phosphate acyltransferase (GPAT), which like MCD and ACC can be regulated by AMP-ac

20 ndrial glycerol-3-phosphate acyltransferase (GPAT)-involved in fatty acid and triacylglycerol synthes

21 d by a glycerol-3-phosphate acyltransferase (GPAT).

22 osomal glycerol-3-phosphate acyltransferase (GPAT).

23 rms of glycerol-3-phosphate acyltransferase (GPAT; E.C. 2.3.1.15) catalyze the committed step in glyc

24 rane-bound, eukaryotic G3P acyltransferases (GPATs) acylate the sn-1 position to produce lysophosphat

25 ed by glycerol-3-phosphate acyltransferases (GPATs).

26 In addition, GPAT promoter-luciferase reporter genes were stimulated

27 lysis revealed that PfGatp is a low affinity GPAT enzyme with a high specificity for C16:0 and C16:1

28 hosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) f

29 malonyl-CoA levels were reduced, and ACC and GPAT activities were diminished by 50% in muscle and liv

30 Consistent with this, GPD and GPAT are highly induced during differentiation of mouse

31 xtends to all subbranches of the Arabidopsis GPAT family.

32 r microsomal acyltransferase enzymes such as GPAT, lysophosphatidic acid acyltransferase (LAT), or ac

33 of the endoplasmic reticulum (ER)-associated GPAT, which accounts for the majority of total GPAT acti

34 genes encoding human and mouse ER-associated GPAT (termed GPAT3).

35 Although the mitochondrial-associated GPAT has been cloned and extensively characterized, the

36 Evidence was obtained for coupling between GPAT and GARS for PRA transfer.

37 significantly contributed to HEK293 cellular GPAT activity.

38 oline in block IV all play a role in E. coli GPAT catalysis.

39 Using Escherichia coli GPAT (PlsB) as a model acyltransferase, we examined the

40 truncated nor loop-tagged enzymes conferred GPAT activity when overexpressed, suggesting that the lo

41 Two distinct GPAT isoenzymes had been identified in mammalian tissues

42 ssue in response to exercise, and except for GPAT, also in muscle.

43 90 degrees C, with half-lives of 65 min for GPAT and 60 h for GARS at 80 degrees C.

44 a critical structural or regulatory role for GPAT function.

45 part for the coordinated expression of human GPAT and AIRC.

46 The human GPAT and AIRC genes are divergently transcribed from a 5

47 These results identify GPATs as partners of fatty acyl oxidases in lipid polyes

48 r oxidation of the acyl group, (2) implicate GPAT specificities as one major determinant of cutin and

49 The increase in GPAT mRNA levels that occurs during differentiation is p

50 A 6-8-fold increase in GPAT-specific activity in the transfected cells confirme

51 served amino acid residues in blocks I-IV in GPAT activity through chemical modification and site-dir

52 st double mutant gat1Deltagat2Delta, lacking GPAT activity.

53 for an endoplasmic reticulum (ER)-localized GPAT for both of these critical metabolic pathways was r

54 sults suggest that GPAT9 is the ER-localized GPAT enzyme responsible for plant membrane lipid and oil

55 Like many GPATs, LpxL can also utilize acyl-CoA as an alternative

56 milar changes in the activities of ACC, MCD, GPAT, and AMPK and the concentration of malonyl-CoA in a

57 In conclusion: MCD, GPAT, and ACC are coordinately regulated by AMPK in live

58 ur data indicate that AGPAT6 is a microsomal GPAT, and we propose renaming this enzyme GPAT4.

59 e endoplasmic reticulum membrane (microsomal GPAT) and an NEM-resistant form in the outer mitochondri

60 rectly correlated to the level of microsomal GPAT enzymatic activity in seeds.

61 Unlike microsomal GPAT, the induced DHAPAT is found to have high activity

62 idine, or lysine did not alter mitochondrial GPAT activity.

63 s transcription of the FAS and mitochondrial GPAT genes, and glucagon antagonizes the insulin effect

64 iptional regulation of FAS and mitochondrial GPAT genes, with emphasis on elucidation of the mechanis

65 een cloned, the microsomal and mitochondrial GPAT isoforms can be distinguished, because they differ

66 lanine and histidine decreased mitochondrial GPAT activity by 90%, replacement with lysine reduced ac

67 d seven amino acid stretch for mitochondrial GPAT activity and the significance of Arg-318 for cataly

68 at Arg-318 may be critical for mitochondrial GPAT activity, whereas Arg-278 can be replaced by a basi

69 of the seven amino acids from mitochondrial GPAT, (312)IFLEGTR(318), which is highly conserved among

70 roximal promoter of the murine mitochondrial GPAT gene bound SREBP-1a and NF-Y in electromobility shi

71 ed the cDNA sequence to murine mitochondrial GPAT.

72 Treatment of mitochondrial GPAT with arginine-modifying agents, phenylglyoxal and c

73 Moreover, R278K mitochondrial GPAT still showed sensitivity to arginine-modifying agen

74 nalysis of the R318K and R318A mitochondrial GPAT showed an 89 and 95%, respectively, decrease in cat

75 a, purified, and reconstituted mitochondrial GPAT activity using phospholipids.

76 synthesis, drastically reduced mitochondrial GPAT activity.

77 stidine, or lysine reduced the mitochondrial GPAT activity by over 90%.

78 he regulated expression of the mitochondrial GPAT gene requires both NF-Y and ADD1/SREBPs.

79 s, as in the case of wild-type mitochondrial GPAT.

80 mido)benzoic acid (15g), possessing moderate GPAT inhibitory activity in an intact mitochondrial assa

81 We conclude that a novel GPAT (mtGPAT2) with antigenic epitopes similar to those

82 MPK activator; 3) changes in the activity of GPAT and ACC paralleled that of MCD; and 4) the increase

83 Hydrophobicity analysis of GPAT predicts two transmembrane domains (TMDs), residues

84 215-260 resulted in decreased expression of GPAT and AIRC in transfected HepG2 cells.

85 ioral analysis showed that RNAi knockdown of GPAT significantly impaired the ability of females to at

86 expressing human AGPAT6 had higher levels of GPAT activity.

87 A substantial loss of GPAT activity in 3T3-L1 adipocytes was achieved by reduc

88 The mRNA of GPAT was increased in islets of obese rats.

89 controls acylation of glycerol 3-phosphate (GPAT) at the sn-1 position.

90 Clearly, plant GPATs can catalyze more reactions than the sn-1 acylatio

91 Purified AGPAT6 protein possessed GPAT activity but not AGPAT activity.

92 s study, which describes the first protozoan GPAT gene, reveals an important role for the endoplasmic

93 mitochondria and mutagenized recombinant rat GPAT (828 aa (amino acids)) expressed in CHO cells.

94 Recombinant GPAT constructs containing tagged epitopes were transien

95 t6-deficient mice exhibited markedly reduced GPAT activity compared with membranes from wild-type mic

96 e results suggested the presence of a second GPAT in liver mitochondria from mtGPAT(-/-) mice.

97 icant increase in N-ethylmaleimide-sensitive GPAT activity, whereas acyltransferase activity toward a

98 ; however, we detected a novel NEM-sensitive GPAT activity in mitochondrial fractions and an anti-mtG

99 When the C terminus and loop-tagged GPAT construct was immunoassayed, the epitope at the C t

100 Our findings indicate that GPAT acts to regulate the biosynthesis of sex pheromone

101 ment of rat liver mitochondria revealed that GPAT has a membrane-protected segment of 14 kDa that cou

102 that this mutation completely abolished the GPAT activity of the recombinant protein.

103 cial point of control of lipid fluxes at the GPAT step is proposed.

104 3-phosphate and fatty acyl-CoA increased the GPAT activity, and the activity was sensitive to N-ethyl

105 ry acyl chains of lipid A are members of the GPAT family and set the stage for structural studies.

106 as co-transfected into the cells or when the GPAT promoter contained mutations in the putative bindin

107 Several of these GPATs are required for the synthesis of cutin or suberin

108 identification of the gene(s) encoding this GPAT activity has remained elusive.

109 Characterization of this GPAT activity in liver from mtGPAT null mice showed that

110 anization, and evolutionary analysis of this GPAT family.

111 oupling involves direct PRA transfer through GPAT-GARS interaction rather than free diffusion.

112 AT, which accounts for the majority of total GPAT activity in most tissues, has remained elusive.

113 Because GPAT4 comprises 65% of the total GPAT activity in brown adipose tissue (BAT), we characte

114 des a glycerol-3-phosphate acyl transferase (GPAT) and is involved in the production of cutin monomer

115 Glycerol-3-PO4 acyl-transferase (GPAT) activity was 12 times that of controls.

116 Unlike GPATs with sn-1 regiospecificity involved in membrane or

117 Similar results were observed when GPAT was truncated before the second TMD, again consiste

118 be associated with microperoxisomes whereas GPAT activity is mainly present in microsomes.

119 ultiple enzymatically distinct proteins with GPAT activity.

120 n this work we studied the role of the yeast GPATs in the formation of LPs induced by a surplus of ol