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1  GPI-PLC by accelerating the conversion of a GPI-PLC.VSG complex to product.
2 tory; the concentration required to activate GPI-PLC 2-fold (SC200) was 310 microM.
3 oacylation, from kinetic analysis, activated GPI-PLC by accelerating the conversion of a GPI-PLC.VSG
4                                     Acylated GPI-PLC was active against variant surface glycoprotein
5 osphatidylinositol-specific phospholipase C (GPI-PLC) from the kinetoplastid Trypanosoma brucei.
6  phosphatidylinositol (GPI)-phospholipase C (GPI-PLC) from the protozoan parasite Trypanosoma brucei,
7 osphatidylinositol-specific phospholipase C (GPI-PLC) have been implicated in releasing the old VSG c
8 osphatidylinositol-specific phospholipase C (GPI-PLC) is an integral membrane protein in the protozoa
9 n of Trypanosoma brucei GPI-phospholipase C (GPI-PLC) results in decreased expression of major surfac
10  mutants are fully viable and (ii) cytosolic GPI-PLC is localized away from cell surface VSG.
11  has been considered unimportant because (i) GPI-PLC null mutants are fully viable and (ii) cytosolic
12 ine plays an important role in activation of GPI-PLC.
13 ibostamycin is not involved in activation of GPI-PLC.
14   Deacylation in vitro decreased activity of GPI-PLC 18-30-fold.
15 cruzi, the in vitro and in vivo behaviors of GPI-PLC-expressing T. cruzi were studied.
16  Leishmania where heterologous expression of GPI-PLC causes a GPI deficiency, the enzyme existed as a
17  in Nonidet P-40, and dimers and monomers of GPI-PLC were the major species in 3-[(3-cholamidopropyl)
18                    Hence, oligomerization of GPI-PLC is associated with high enzyme activity both in
19     The basis for the apparent quiescence of GPI-PLC is not known.
20 chanism for post-translational regulation of GPI-PLC activity.
21 oss-linking revealed the oligomeric state of GPI-PLC during latency and after enzyme activation.
22               The half-life of fatty acid on GPI-PLC was 45 min, signifying the dynamic nature of the
23                         Neomycin B protected GPI-PLC from a reduction in activity at pH 6.5, and incr
24  monomers, dimers, and tetramers of purified GPI-PLC was detected by molecular sieving and shown to b
25                                   Tetrameric GPI-PLC was 3.6-20-fold more active than the monomeric e
26                       Assembly of tetrameric GPI-PLC occurred when parasites were subjected to condit
27 erentiation has multiple mechanisms and that GPI-PLC plays a more significant role in VSG release tha
28   Surface biotinylation assays indicate that GPI-PLC does gain access to extracellular VSG, suggestin
29                                          The GPI-PLC gene in Trypanosoma brucei encodes a phospholipa
30 rted that the differential expression of the GPI-PLC mRNA also results from a 10-fold difference in h
31 specifically blocking the translation of the GPI-PLC mRNA in procyclic forms by the inclusion of a ha
32               Second, the instability of the GPI-PLC mRNA in procyclic forms can be reversed by the i
33 tein synthesis inhibitors in stabilizing the GPI-PLC mRNA operates in trans through a short-lived fac

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