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   3 oacylation, from kinetic analysis, activated GPI-PLC by accelerating the conversion of a GPI-PLC.VSG 
  
  
     6  phosphatidylinositol (GPI)-phospholipase C (GPI-PLC) from the protozoan parasite Trypanosoma brucei,
     7 osphatidylinositol-specific phospholipase C (GPI-PLC) have been implicated in releasing the old VSG c
     8 osphatidylinositol-specific phospholipase C (GPI-PLC) is an integral membrane protein in the protozoa
     9 n of Trypanosoma brucei GPI-phospholipase C (GPI-PLC) results in decreased expression of major surfac
  
    11  has been considered unimportant because (i) GPI-PLC null mutants are fully viable and (ii) cytosolic
  
  
  
  
    16  Leishmania where heterologous expression of GPI-PLC causes a GPI deficiency, the enzyme existed as a
    17  in Nonidet P-40, and dimers and monomers of GPI-PLC were the major species in 3-[(3-cholamidopropyl)
  
  
  
  
  
  
    24  monomers, dimers, and tetramers of purified GPI-PLC was detected by molecular sieving and shown to b
  
  
    27 erentiation has multiple mechanisms and that GPI-PLC plays a more significant role in VSG release tha
    28   Surface biotinylation assays indicate that GPI-PLC does gain access to extracellular VSG, suggestin
  
    30 rted that the differential expression of the GPI-PLC mRNA also results from a 10-fold difference in h
    31 specifically blocking the translation of the GPI-PLC mRNA in procyclic forms by the inclusion of a ha
  
    33 tein synthesis inhibitors in stabilizing the GPI-PLC mRNA operates in trans through a short-lived fac
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