ライフサイエンスコーパス: GRalpha

1 GRalpha and GRbeta transcripts are coordinately upregula

2 GRalpha undergoes steroid-dependent nuclear translocatio

3 veness by inhibiting steroid induced both 1) GRalpha-DNA binding activity and 2) GC-responsive elemen

4 ly increased by TNFalpha and IFNgamma with a GRalpha/GRbeta ratio of 1 to 3.

5 acological doses of L-carnitine can activate GRalpha and, through this mechanism, regulate glucocorti

6 ch competes with and thus inhibits activated GRalpha; increased secretion of macrophage migration inh

7 or the nuclear transport of ligand-activated GRalpha.

8 ), were tested for their abilities to affect GRalpha trafficking.

9 the isoforms glucocorticoid receptor alpha (GRalpha) and glucocorticoid receptor beta (GRbeta), and

10 tive levels of the active GC receptor alpha (GRalpha) and the alternatively spliced dominant negative

11 TCF, JunD and glucocorticoid receptor alpha (GRalpha), that show different enrichment patterns for pe

12 Glucocorticoid receptor-alpha (GRalpha) and peroxisome proliferator-activated receptor-

13 -carnitine on glucocorticoid receptor-alpha (GRalpha) functions.

14 We have analyzed GRalpha and 11beta-HSD1 expression in skeletal myoblasts

15 induced nuclear factor kappaB activation and GRalpha phosphorylation, which were prevented by inhibit

16 tation experiments revealed that MUC1-CT and GRalpha form protein complexes and translocate to the nu

17 ession in vivo by both GRalpha-dependent and GRalpha-independent mechanisms.

18 in kinase phosphatase 1 gene expression, and GRalpha nuclear translocation.

19 ional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner.

20 Physiologically, hGRbeta antagonized GRalpha's function and attenuated hepatic gluconeogenesi

21 h does not bind known ligands and attenuates GRalpha action.

22 Both GRalpha transactivation and cytokine suppression by L-ca

23 nts of liver gene expression in vivo by both GRalpha-dependent and GRalpha-independent mechanisms.

24 ed in constitutive nuclear transport of both GRalpha and -beta in the absence of ligands.

25 fully reversed rhinovirus impairment of both GRalpha nuclear translocation and the transactivation/tr

26 lls were used to show binding of PP5 to both GRalpha and PPARgamma.

27 A 1757-bp 3' fragment of bovine GRalpha cDNA was also amplified from bovine lens.

28 as dominant negative regulator of classical GRalpha transcriptional activity.

29 The complete GRalpha sequence was obtained from rLECs and was found t

30 MUC1-CT-GRalpha complex was downregulated in NP-CR tissue.

31 he human glucocorticoid receptor (GR) exist, GRalpha and GRbeta, which arise from alternative splicin

32 (MFI) using immunofluorescence analysis for GRalpha was 475 +/- 62 and 985 +/- 107 for PBMCs and neu

33 protein isoforms: a cytoplasmic alpha form (GRalpha), which binds hormone, translocates to the nucle

34 promoter and on the expression of functional GRalpha by the cell.

35 ped localize green fluorescent protein-fused GRalpha in the cytoplasm in the absence of ligand and po

36 ling is coordinated via the GC receptor (GR) GRalpha, as the GRbeta isoform lacks a ligand-binding do

37 Increased GRbeta-GRalpha ratios were associated with decreased DEX-mediat

38 comatous TM cell strains have a lower GRbeta-GRalpha ratio compared to normal TM cells, making them m

39 e used to study the effect of TSTs on GRbeta-GRalpha ratios in human TM cell strains.

40 TSTs increased the GRbeta-GRalpha ratio in TM cells.

41 green fluorescent protein (GFP)-fused human GRalpha and transactivate the glucocorticoid-responsive

42 human ERK8 as a negative regulator of human GRalpha, acting through Hic-5, and suggest a broader rol

43 This was associated with impaired GRalpha nuclear translocation, as assessed by means of b

44 Importantly, GRalpha expression was unaffected by variations in in vi

45 No change in GRalpha expression was observed.

46 romoter that leads to a 1.5-fold increase in GRalpha mRNA and a 2.0-fold increase in GRbeta mRNA in H

47 emokine production and its ability to induce GRalpha nuclear translocation and GRE-dependent GILZ exp

48 Fluticasone-induced GRalpha nuclear translocation, phosphorylation at serine

49 ly, through its 14-3-3 protein interactions, GRalpha may influence these processes.

50 ntaining the N-terminal truncated GR isoform GRalpha-D3 lacking the K310 SUMOylation site, revealed a

51 ive splicing, the GR exists as two isoforms, GRalpha and GRbeta.

52 in the corticosteroid response that mediates GRalpha nuclear translocation.

53 teroid resistance process, inhibiting normal GRalpha nuclear function.

54 ly modulates the lipometabolic activities of GRalpha and PPARgamma.

55 o suppressed the transcriptional activity of GRalpha on a glucocorticoid-responsive promoter.

56 trafficking and transactivation activity of GRalpha, whereas introduction of a mutation inactivating

57 ly influence the transcriptional activity of GRalpha.

58 The concentration of GRalpha mRNA is more than a 1000-fold higher than that f

59 -3-3sigma bound the ligand-binding domain of GRalpha through its COOH-terminal portion, in a partiall

60 at raised skeletal muscle cell expression of GRalpha and 11beta -HSD1-mediated regulation of intracel

61 is was performed to detect the expression of GRalpha and GRbeta in TM cells and its regulation by dex

62 were evident between myoblast expression of GRalpha under basal conditions and levels of insulin res

63 wn and potential new biological functions of GRalpha.

64 PP5-dependent impairment of GRalpha function represents a novel mechanism driving GC

65 bind glucocorticoids and is an inhibitor of GRalpha activity.

66 Increased levels of GRalpha/GRbeta heterodimers were found in neutrophils as

67 Loss of PP5 increased phosphorylation of GRalpha at serines 212 and 234 and elevated dexamethason

68 shuttling and transactivation properties of GRalpha in a cell line replete with or devoid of 14-3-3s

69 Regulation of GRalpha and 11beta-HSD1 by cortisol was abolished by the

70 Transcriptional regulation of GRalpha and interaction with its ligand binding domain b

71 DEX stimulated the translocation of GRalpha but not GRbeta.

72 6 did not alter DEX-induced translocation of GRalpha.

73 te SRp levels and determine their effects on GRalpha/GRbeta ratios as well as dexamethasone (DEX) res

74 lities that have been reported for GRbeta on GRalpha.

75 te levels of the GRbeta protein isoform over GRalpha, making GRbeta the predominant endogenous recept

76 cle (ASM) caused by a defect in GC receptor (GRalpha) function.

77 ression of the two glucocorticoid receptors, GRalpha and GRbeta.

78 increase steroid responsiveness by reversing GRalpha phosphorylation.

79 ly transfected red fluorescent protein (RFP)-GRalpha NTM5 cell lines were developed.

80 RFP-GRalpha receptor behaves similarly to the wild-type GRal

81 RFP-GRalpha translocation was temperature sensitive, occurri

82 on; however, 17AAG prevented DEX-induced RFP-GRalpha nuclear translocation.

83 ine blocked DEX-induced or RU486-induced RFP-GRalpha nuclear translocation; however, 17AAG prevented

84 nuclear import and export of DEX-induced RFP-GRalpha were faster than RU-486-induced nuclear shuttlin

85 Nuclear localization of RFP-GRalpha in NTM5 cells treated with vehicle (ethanol), de

86 Nuclear export of RFP-GRalpha was studied using confocal microscopy following

87 ad no effect on nuclear translocation of RFP-GRalpha.

88 NTM5 cells transfected with RFP-GRalpha showed a clear cytosolic localization of recepto

89 These studies suggest that GRalpha transcripts containing exons 1A3, 1B, and 1C con

90 The GRalpha in hLECs and rLECs was shown to be transcription

91 ression by L-carnitine were abrogated by the GRalpha-antagonist RU 486.

92 This inversion of the GRalpha/GRbeta ratio in human neutrophils compared with

93 nondenaturing conditions, we found that the GRalpha isoform coprecipitates with NF-ATc in nuclear ex

94 When equal amounts of the cDNAs for these GRalpha variant proteins are transfected into cells, the

95 e whole cell binding of [3H]dexamethasone to GRalpha by decreasing the affinity of this receptor for

96 receptor behaves similarly to the wild-type GRalpha with its cytosolic localization and shuttling to

97 the GC-induced signaling during fasting via GRalpha and the PPARalpha-FGF21 axis that reduces fat bu

98 h inhibitory actions of GCs are mediated via GRalpha, a hormone-induced transcription factor.

99 revealed that MUC4beta forms a complex with GRalpha in the nuclei of NP epithelial cells from cortic

100 form transcriptionally active complexes with GRalpha, whereas in PV EBs, STAT-5 was constitutively ph

101 study interactions of FKBP51 and FKBP52 with GRalpha, GRbeta, Hsp90, or dynein.