ライフサイエンスコーパス: Gag protein

1 oviruses and expresses aberrant forms of the Gag protein.

2 particles are assembled from the multidomain Gag protein.

3 from the human immunodeficiency virus (HIV) Gag protein.

4 s toxoid precursor, EBV nuclear Ag 2, or HIV gag protein.

5 ecombinant vaccinia virus expressing the HIV Gag protein.

6 ractions capable of reversibly extending the Gag protein.

7 A), nucleocapsid protein (NC), or entire DP6-Gag protein.

8 ecombinant vaccinia virus expressing the HIV Gag protein.

9 ized "buttons" containing oligomerized viral Gag protein.

10 orphisms present in the CA-SP1 region of the Gag protein.

11 large areas of the VLP membrane are void of Gag protein.

12 combined with antibody staining for the HIV Gag protein.

13 cytosolic immature and endocytic mature HIV gag protein.

14 C terminus of HIV p17 matrix within the HIV gag protein.

15 found in the equine infectious anemia virus Gag protein.

16 rovirus particle assembly is mediated by the Gag protein.

17 d region within the CA and NC regions of the Gag protein.

18 treatment of cells expressing the wild-type Gag protein.

19 cell responses when administered with HIV-1 Gag protein.

20 uces important conformational changes in the Gag protein.

21 n primates (NHPs) when administered with HIV Gag protein.

22 could be rescued by coexpression of untagged Gag protein.

23 and stabilization of the RNA dimer requires Gag protein.

24 domain of human immunodeficiency virus (HIV) gag protein.

25 V particles, a process directed by the viral Gag protein.

26 d with the amphotropic envelope or the glyco-Gag protein.

27 cleocytoplasmic transport properties of both Gag proteins.

28 ate nucleocapsid domains of assembling viral Gag proteins.

29 gnate nucleocapsid (NC) domains of the viral Gag proteins.

30 iruses in human genome today have functional Gag proteins.

31 ation, we created viruses bearing NES mutant Gag proteins.

32 tein products contain homology to retroviral Gag proteins.

33 e representative Beta- and Epsilonretrovirus Gag proteins.

34 Mamu-B*01-restricted epitopes in the Env and Gag proteins.

35 s with the MA domains of the viral precursor Gag proteins.

36 human immunodeficiency virus type 1 (HIV-1) Gag proteins.

37 a role in PM targeting of several retroviral Gag proteins.

38 is critical for PM association of retroviral Gag proteins.

39 emonstrate that SPTBN1 associates with HIV-1 gag proteins.

40 (gag), which recognize the virus encoded p24(gag) protein.

41 gag), the precursor for internal structural (Gag) proteins.

42 the nucleocapsid (NC), spacer 2 (SP2), or p6(Gag) proteins.

43 in the position of NC was a nonmyristoylated Gag protein able to multimerize.

44 membrane, their assembly rate accelerated as Gag protein accumulated in cells, and typically 5-6 min

45 of an MLV vector, we hypothesized that other Gag proteins act cooperatively with p12 during the early

46 RSV Gag protein adopts a more tightly curved lattice than is

47 es, compared with animals immunized with HIV Gag protein alone.

48 with both the encoded Matrix (MA) domain of Gag protein and 5' UTR of the translating mRNA and promo

49 NHPs immunized with HIV Gag protein and a TLR7/8 agonist or a TLR9 ligand [CpG o

50 rNDV vector expressing a codon-optimized HIV Gag protein and demonstrated its ability to induce a Gag

51 We have purified Rous sarcoma virus (RSV) Gag protein and in parallel several truncation mutants o

52 human immunodeficiency virus type 1 (HIV-1) Gag protein and that their interaction is important for

53 efore, understanding the processing of HIV-1 Gag protein and the resulting epitope repertoire is esse

54 se, compared with animals immunized with HIV Gag protein and the TLR7/8 agonist or CpG ODN.

55 ions in the steady-state levels of the HIV-1 Gag protein and virus production; Mov10 was efficiently

56 the nucleocapsid domains (NC) of assembling Gag proteins and direct their packaging into virus parti

57 ble by SEM and compared these numbers for WT Gag proteins and for Gag proteins that were blocked at t

58 escape, varies widely even in the conserved Gag proteins and suggest that differential escape costs

59 Western blot assays using recombinant viral Gag protein, and an indicator cell line that can detect

60 with broad CD8(+) T cells that targeted the Gag protein, and CD8(+) T cells from these individuals e

61 detection of gagpol mRNA, intracellular p24 Gag protein, and cell surface markers.

62 ognition of discrete epitopes largely in the Gag protein, and expansion of IL-21(+) CD4(+) T cells in

63 human immunodeficiency virus type 1 (HIV-1) Gag protein, and the same mRNAs were enriched in MLV and

64 sition as well as levels of IAP transcripts, Gag proteins, and reverse transcription products.

65 linked target sites in the transmitted virus Gag protein are already adapted, and that this transmitt

66 oxic T lymphocytes [CTLs]) against the HIV-1 Gag protein are associated with control among those with

67 T lymphocyte (CTL) responses against the HIV Gag protein are associated with lowering viremia; howeve

68 ing and high-affinity binding to the cognate Gag protein are exposed in helical junctions.

69 In these particles, the Gag proteins are arranged radially as elongated rods.

70 perimental results strongly suggest that RSV Gag proteins are capable of interacting within the nucle

71 for the heterologous RNA copackaging; HIV-1 Gag proteins are capable of mediating HIV-1 and HIV-2 RN

72 virus (RSV) and murine leukemia virus (MLV) Gag proteins are selectively recruited to these structur

73 In vitro, recombinant Gag proteins are soluble but assemble into virus-like pa

74 Retroviral Gag proteins are synthesized as soluble, myristoylated p

75 For Rous sarcoma virus (RSV), Gag proteins are synthesized in the cytoplasm and then u

76 crophages, both wild-type (WT) and MA mutant Gag proteins are targeted specifically to the MVB.

77 1) replication, newly synthesized retroviral Gag proteins are targeted to lipid raft regions of speci

78 of retroviral replication, newly synthesized Gag proteins are targeted to the plasma membrane (PM), w

79 1) replication, newly synthesized retroviral Gag proteins are targeted to the plasma membrane of most

80 rus (RSV) capsid protein is cleaved from the Gag protein as the proteolytic intermediate CA-SP.

81 proteins that came in close proximity to the Gag proteins as they were synthesized, transported, asse

82 human immunodeficiency virus type 1 (HIV-1) Gag protein assembly in vitro, we analyzed the assembly

83 approaches have illuminated the dynamics of Gag protein assembly, viral RNA packaging and ESCRT (end

84 esults suggest a multistep pathway for HIV-1 Gag protein assembly.

85 ering with proteolytic cleavage of the viral Gag protein at a specific site.

86 ar export, thereby isolating these "trapped" Gag proteins at an early assembly step.

87 constructed a series of models of the mutant Gag protein based on these domain structures, and tested

88 Confocal imaging of wild-type and mutant Gag proteins bearing different fluorescent tags suggeste

89 like particles produced by the expression of Gag proteins bearing p10 mutations were also aberrant, w

90 Gag proteins bearing the nucleoplasmin NLS insertion dis

91 oteins colocalized extensively with chimeric Gag proteins bearing the same CA domain, implying that G

92 proteins, but, surprisingly, only the mutant Gag protein biotinylated the HIV-1 Envelope protein.

93 able to study how monovalent and multivalent GAG.protein bonds respond to directed mechanical forces.

94 lly similar to R-848 was conjugated to HIV-1 Gag protein both Th1 and CD8(+) T cells responses were e

95 he Gag-Pro-Pol precursor encodes most of the Gag protein but now includes the viral enzymes: protease

96 s encoded by the HIV-1 pol gene and neighbor Gag proteins, but, surprisingly, only the mutant Gag pro

97 ost conserved segment in the highly variable Gag protein called the major homology region (MHR).

98 HIV Gag protein can be directly detected in infected resting

99 Purified retroviral Gag proteins can assemble in vitro to form immature viru

100 ompletely cleaved) CA and that CA-containing Gag proteins can be cleaved by the viral protease in SIV

101 sfection system in which epitopically tagged Gag proteins can be traced back to their mRNA origins in

102 thogens, we examined whether HIV-1 and HIV-2 Gag proteins can coassemble and functionally complement

103 cts were alleviated by the coexpression of a Gag protein carrying a wild-type (WT) NC domain but devo

104 th a detection limit of 0.5-1 Gagpol mRNA(+)/Gag protein(+) cells per million CD4 T cells.

105 primer binding site through a portion of the gag protein coding region.

106 However, wild-type Gag proteins colocalized extensively with chimeric Gag p

107 rap assay, based on co-expression of the HIV Gag protein, confirmed that this post-translational modi

108 Many retroviral Gag proteins contain PPXY late assembly domain motifs th

109 Retroviral Gag proteins contain short late-domain motifs that recru

110 n efficiency for each of the three telomeric Gag proteins correlates with the relative abundance of t

111 tion experiments revealed that the wild-type Gag protein could partially rescue export-defective Gag

112 In contrast, a single natural Gag protein covered only 37% (9 of 9) and 67% (8 of 9).

113 ion at early stages of infection, proceeding Gag protein detection.

114 The Capsid region of the viral Gag protein dictates susceptibility to MX2, and the bloc

115 Gag proteins direct the process of retroviral particle a

116 Retroviral Gag proteins direct virus particle assembly from the pla

117 The mouse mammary tumor virus (MMTV) Gag protein directs the assembly in the cytoplasm of imm

118 Thus, the mature FV Gag protein does not consist of the matrix, capsid, and

119 l genomic RNAs are encapsidated by the viral Gag protein during virion assembly.

120 f HIV-1 infection with IgG Abs against HIV-1 Gag proteins (e.g., p24) and/or production of IgG2 Abs a

121 NHP were immunized with HIV Gag protein emulsified in Montanide ISA 51, an oil-based

122 Rhesus macaques (RMs) were primed with SIV Gag protein emulsified in Montanide ISA51 with or withou

123 ractions between the RNA dimer and the viral Gag protein ensure selective packaging into nascent imma

124 The retroviral Gag protein exhibits extensive amino acid sequence varia

125 human immunodeficiency virus type 1 (HIV-1) Gag protein expressed in COS-1 cells using fluorescence

126 portion of the simian immunodeficiency virus Gag protein expressed in mice by an L1-modified rMRV was

127 silencing the m(6)A writers decreased HIV-1 Gag protein expression in virus-producing cells, while s

128 in vivo experiments revealed that higher HIV Gag protein expression positively correlates with an enh

129 losis vaccine strains expressing SIV Env and Gag proteins, followed by systemic heterologous (MVA-SIV

130 vectors encoding several RSV/HIV-1 chimeric Gag proteins for expression in either insect cells or ve

131 his model, we took advantage of a retroviral Gag protein from the prototypic foamy virus (PFV) that i

132 factor (Rej) necessary for the synthesis of Gag protein from unspliced viral RNA.

133 to study the cytoplasmic interactions of the Gag proteins from human immunodeficiency virus type 1 (H

134 analyzed the nuclear trafficking ability of Gag proteins from six retroviral genera.

135 Gag proteins from two other retroviruses gave similar re

136 types having AKV Env and Moloney MLV (MoMLV) Gag proteins, further indicating that AKV Env sequence v

137 was administered alone or together with HIV Gag protein (Gag), and the magnitude, quality, and pheno

138 ically detected a tetracysteine-tagged HIV-1 Gag protein (Gag-TC) in HeLa, Mel JuSo, and Jurkat T cel

139 HIV-1 Gag protein gains access to ESCRT directly by binding Al

140 virus (MuLV) that utilizes its glycosylated Gag protein (gGag) to evade APOBEC3.

141 ce, we demonstrate that the MLV glycosylated Gag protein (glyco-Gag) enhances viral core stability.

142 lycans (PGs), a family of glycosaminoglycan (GAG)-protein glycoconjugates, contribute to animal physi

143 further suggesting that, compared to NC, the Gag protein has a greater propensity to affect RNA struc

144 Previously, no retroviral Gag protein has been highly purified in milligram quanti

145 The Gag protein has been shown to interact with other Gag pr

146 For HIV-1, the Gag protein has the role of a polyprotein precursor that

147 4+ T cell immunity relative to high doses of gag protein, HIV gag plasmid DNA, or recombinant adenovi

148 ngest association with response to the HIV-1 Gag protein: HLA-B alleles known to be associated with d

149 eptide 1 (SP1), the portion of the precursor Gag protein immediately C terminal to the CTD.

150 APOBEC3B specifically interact with the IAP Gag protein in co-expressing cells, and induce extensive

151 n to interact and co-localize with the HIV-1 Gag protein in infected cells.

152 Expression of a retroviral Gag protein in mammalian cells leads to the assembly of

153 es when used as vaccine adjuvants with HIV-1 Gag protein in mice.

154 human immunodeficiency virus type 1 (HIV-1) Gag protein in solution adopts compact conformations.

155 aM co-localizes and interacts with the HIV-1 Gag protein in the cytosol of infected cells.

156 rities between these host proteins and viral Gag protein in the nature of their PI(4,5)P2 interaction

157 , we detected HERVK viral-like particles and Gag proteins in human blastocysts, indicating that early

158 s producing these mutants showed assembly of Gag proteins in large, flat or dome-shaped patches at th

159 mmunodeficiency virus [FIV]) vis-a-vis their Gag proteins in live cells.

160 tion and the appearance of new ubiquitinated Gag proteins in virions.

161 itro, we analyzed the assembly properties of Gag proteins in which NC domains were replaced with cyst

162 ciated with increased in vitro expression of Gag protein, in vivo expression of Gag mRNA, and enhance

163 enabling detection of 0.5-1 gag-pol mRNA(+)/Gag protein(+)-infected cells per million.

164 which the MA domains of different retroviral Gag proteins influence gRNA packaging, highlighting vari

165 HIV-1 Gag protein interaction with cyclophilin A (CypA) is cri

166 owledge, class of inhibitors that target the GAG-protein interaction.

167 sights into the nature of glycosaminoglycan (GAG)-protein interactions and prove useful for optimizat

168 GAG-protein interactions participate in neuronal develop

169 gate numerous other physiologically relevant GAG-protein interactions.

170 le method for analyzing the nanomechanics of GAG.protein interactions at the level of single GAG chai

171 finding to humans, we introduced the HIV p24 gag protein into a mAb that targets DEC-205/CD205, an en

172 membrane binding and assembly of retroviral Gag proteins into a lattice are understood.

173 HIV-1 Gag protein is a component of several experimental HIV-1

174 human immunodeficiency virus type 1 (HIV-1) Gag protein is a major target antigen for cytotoxic-T-ly

175 The capsid domain (CA) of the retroviral Gag protein is a primary determinant of Gag oligomerizat

176 The viral Gag protein is believed to chaperone tRNA(3)(Lys) placem

177 ar transport of the Rous sarcoma virus (RSV) Gag protein is intrinsic to the virus assembly pathway.

178 The matrix domain (MA) of HIV-1 Gag protein is N-myristoylated and plays an important ro

179 e N-terminal matrix (MA) domain of the HIV-1 Gag protein is responsible for binding to the plasma mem

180 Since the Gag protein is the central component for the production

181 The Gag protein is the main retroviral structural protein, a

182 The Gag protein is the major structural determinant of retro

183 erved that the coassembly of HIV-1 and HIV-2 Gag proteins is not required for the heterologous RNA co

184 her complicated when group-specific antigen (Gag) protein is expressed, because a significant portion

185 er, some simian immunodeficiency virus (SIV) Gag proteins lack this consensus sequence, yet still bin

186 HIV-1 particles constructed from recombinant Gag proteins lacking residues 16-99 and the p6 domain as

187 ein interactions are greatly diminished when Gag proteins lacking the I domain are expressed.

188 human immunodeficiency virus type 1 (HIV-1) Gag protein (lacking myristate at its N terminus and the

189 When recombinant HIV-1 Gag protein (lacking myristate at its N terminus and the

190 vitro, RNA aptamers raised against an HIV-1 Gag protein, lacking the N-terminal myristate and the C-

191 stimulate HIV-1 release but in this case the Gag protein lacks a PPXY motif, suggesting that NEDD4L m

192 Expression of the retroviral Gag protein leads to formation of virus-like particles i

193 immature capsid and domains of its component Gag proteins, less is known about the sequence of events

194 radation, resulting in reduced intracellular Gag protein levels; this phenotype was rescued by reintr

195 ching (FRAP) revealed that the population of Gag proteins localized within YB-1 complexes was relativ

196 that phosphorylation of these CA-containing Gag proteins may require an environment that is unique t

197 The MA domain of the retroviral Gag protein mediates interactions with the plasma membra

198 The MA domain of retroviral Gag proteins mediates association with the host cell mem

199 virions shows that in these particles, HIV-1 Gag protein molecules are rod shaped.

200 nd its effects upon the interactions between Gag protein molecules in solution.

201 Animals vaccinated with HIV Gag protein/Montanide and CpG ODN or the TLR7/8 agonist

202 Gag proteins must extensively multimerize during the for

203 tags suggested that complementation between Gag proteins occurred in the nucleus.

204 hly conserved motif that is found within the Gag protein of all orthoretroviruses and some retrotrans

205 hen it is transplanted into the heterologous Gag protein of equine infectious anemia virus (EIAV).

206 The myristylated full-length Gag protein of HIV-1 was purified to monodispersity.

207 ector of human serotype 5 (AdHu5) expressing Gag protein of HIV-1, in the presence or absence of pree

208 ic cell (DC)-directed LV system encoding the Gag protein of human immunodeficiency virus (HIV) (LV-Ga

209 The Gag protein of human immunodeficiency virus type 1 (HIV-

210 The Gag protein of human immunodeficiency virus type 1 direc

211 s a cellular protein that interacts with the Gag protein of Mason-Pfizer monkey virus.

212 We now describe the properties of the Gag protein of Moloney murine leukemia virus (MLV), a ga

213 the identification of a novel domain in the Gag protein of Moloney murine leukemia virus (MoLV) that

214 Before membrane transport, the multidomain Gag protein of Rous sarcoma virus (RSV) undergoes import

215 The Gag protein of the murine retrovirus mouse mammary tumor

216 dition, we observed that in cells expressing Gag proteins of both viruses, HERV-K(CON) Gag colocalize

217 Altogether, these results indicate that Gag proteins of endogenous retroviruses can coassemble w

218 We show that 1) ORF1 and Gag proteins of retrotransposons contain high amounts of

219 te domain motifs have also been found in the Gag proteins of retroviruses and the matrix proteins of

220 The Gag proteins of Rous sarcoma virus (RSV) and human immun

221 matitis virus (VSV) vectors encoding Env and Gag proteins of simian immunodeficiency virus-human immu

222 ent, ALIX-binding late domains within the p6(Gag) proteins of SIV(mac239) ((40)SREKPYKEVTEDLLHLNSLF(5

223 To examine the effect of Gag protein on HIV-1 RNA transport, we analyzed the cyto

224 Localization of the HIV type-1 (HIV-1) Gag protein on the plasma membrane (PM) for virus assemb

225 ys residues showed that the contacts between Gag proteins on the membrane are similar to the known co

226 determined that despite encoding a truncated Gag protein, only the full-length Gag protein was incorp

227 The HIV-1 Gag protein orchestrates all steps of virion genesis, in

228 A subgenomic Ty1 mRNA encodes a truncated Gag protein (p22) that is cleaved by Ty1 protease to for

229 How the Gag proteins pack together in the lattice is incompletel

230 nism, referred to as cis packaging, in which Gag proteins package the RNA from which they were transl

231 In the first step, Gag protein pairing through NC-RNA interactions or C-ter

232 In accordance with the model that Gag protein pairing triggers assembly, we found that cys

233 The Gag protein plays a crucial role in the assembly of retr

234 After priming, RMs that received SIV Gag protein plus poly-IC developed significantly higher

235 ignificantly increased in RM primed with SIV Gag protein plus poly-IC, with or without the TLR7/8 lig

236 strate that prime-boost vaccination with SIV Gag protein/poly-IC improves magnitude, breadth, and dur

237 MA) that lies at the N-terminus of the viral Gag protein precursor appears to be one of the crucial s

238 occurs prior to encapsidation and that HIV-2 Gag proteins primarily package one dimeric RNA rather th

239 llenge was not significantly enhanced by SIV Gag protein priming with any of the adjuvants.

240 ) T cell response in BAL was enhanced by SIV Gag protein priming with poly-IC or CpG, which correlate

241 icing phenotype, we show that the effects on Gag protein processing and virus particle production of

242 hown to affect virus particle production and Gag protein processing.

243 nd Epsilonretrovirus Gag proteins, the other Gag proteins produced VLPs as confirmed by TEM, and morp

244 ette in the envelope gene were defective for Gag protein production and the nuclear export of unsplic

245 tes NF-kappaB, significantly augmented viral Gag protein production in XMRV-infected cells.

246 the nuclear export mutants, we asked whether Gag protein-protein interactions occur within the nucleu

247 mediated by the myristoylated N terminus of Gag, protein-protein interactions between CA domains, an

248 nfidence interval, 0.89-1.05]) and lower for Gag proteins (ratio, 0.67 [95% confidence interval, 0.62

249 human immunodeficiency virus type 1 (HIV-1) Gag protein recruits Tsg101 to facilitate HIV-1 particle

250 as severely diminished even though NC mutant Gag proteins retained the ability to assemble spherical

251 release from 293T cells, although NC mutant Gag proteins retained the ability to interact with cellu

252 Since NC mutant Gag proteins retained the interaction with Tsg101, we co

253 the role(s) of the alternative ORF2-derived gag protein(s) of BM5def in viral pathogenesis.

254 tion inhibitors are novel as they target the Gag protein, specifically by inhibiting CA-SP1 proteolyt

255 ag) method is 1,000-fold more sensitive than Gag protein staining alone, with a detection limit of 0.

256 l-length JSRV expression construct abolished Gag protein synthesis and released viruses in 293T cells

257 pacts on FRET between normally myristoylated Gag proteins than do CA-CTD mutations.

258 The only Gag protein that displayed CRM1-dependent nuclear cyclin

259 mains) are short amino acid sequences in the Gag protein that facilitate the process of budding.

260 ium in solution, and have described a mutant Gag protein that remains monomeric at high concentration

261 esistant to multiple PR inhibitors or mutant Gag proteins that confer resistance to the maturation in

262 us particles, requiring interactions between Gag proteins that form a protein layer under the viral m

263 , virus-like particles can be generated from Gag proteins that lack the N-terminal myristic acid modi

264 ed these numbers for WT Gag proteins and for Gag proteins that were blocked at the last step in buddi

265 In the Rous sarcoma virus (RSV) Gag protein, the 25 amino-acid residues of the p10 domai

266 e representative Beta- and Epsilonretrovirus Gag proteins, the other Gag proteins produced VLPs as co

267 ively, our results show that conjugating HIV Gag protein to a TLR7/8 agonist is an effective way to e

268 to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs.

269 Importantly, conjugating the HIV Gag protein to the TLR7/8 agonist (Gag-TLR7/8 conjugate)

270 , extended scaffold that connects retroviral Gag proteins to ESCRT-III and other cellular-budding mac

271 Our results offer new insights into HIV-1 Gag protein trafficking and activities and provide new p

272 ated proteins offers new insights into HIV-1 Gag protein trafficking and activities and provides new

273 discovered that the Rous sarcoma virus (RSV) Gag protein transiently localizes to the nucleus, althou

274 at in S6-expressing cells, although Gag(Pr55(gag)) protein translation was unaffected, processing and

275 OBEC3 packaging in the virion, the MLV glyco-Gag protein uses a unique mechanism to counteract the an

276 the assembly process and characterizing the Gag protein using neutron scattering, we have identified

277 ponses to a dendritic cell (DC)-targeted HIV gag protein vaccine in mice.

278 overy study of 364 peptides from three HIV-1 Gag protein variants.

279 d 12 h after stimulation with RMD, while p24 Gag protein was detected for the first time after 18 h p

280 truncated Gag protein, only the full-length Gag protein was incorporated into virus particles.

281 The localization of Gag proteins was examined in the wild type and in mutant

282 ference in cross-clade reactivity to Nef and Gag proteins was significant (P<.0001).

283 esis/assembly system for chimeric HIV type 1 Gag proteins, we have replicated the activity of PA-457

284 en residues on capsid and matrix of the same Gag protein were often due to structural proximity.

285 Wild-type and chimeric Gag proteins were capable of coassembly into a single VL

286 flotation analyses, the intact RSV and HIV-1 Gag proteins were more similar to multimerized MA than t

287 Epitopes within Nef and Gag proteins were the most commonly recognized in both v

288 d by several thousand molecules of the viral Gag protein, which assemble to form the known hexagonal

289 human immunodeficiency virus type 1 (HIV-1) Gag protein, which directs viral assembly and release, a

290 yze the interactions of the HIV-1 structural Gag proteins, which involved tagging wild-type and mutan

291 The Gag protein with a mutation at the dimer interface also

292 alternative version of the structural viral Gag protein with an extra upstream region that provides

293 inking nuclear trafficking of the retroviral Gag protein with gRNA incorporation.

294 which involved tagging wild-type and mutant Gag proteins with a biotin ligase.

295 and protein expression levels indicated that Gag proteins with a chimeric HIV-1 CA NTD/HTLV-1 CA CTD

296 enesis, we generated and analyzed a panel of Gag proteins with chimeric HIV-1/HTLV-1 CA domains.

297 Furthermore, chimeric Gag proteins with the HTLV-1 CA NTD produced particles p

298 rotein has been shown to interact with other Gag proteins, with the viral RNA, and with the cell memb

299 bic residues resulted in the accumulation of Gag proteins within the nucleus and a budding defect gre

300 rease in fluorescence, while addition of the Gag protein yielded a large change in fluorescence, furt