ライフサイエンスコーパス: Glu-tRNA

1 rm the intermediate indicates that GatE is a Glu-tRNA(Gln) kinase.

2 In the absence of the amido acceptor, Glu-tRNA(Gln), the enzyme has basal glutaminase activity

3 ed a similar discriminatory response against Glu-tRNA (Gln).

4 s, including plastid DNA repair proteins and Glu tRNA reductase, which is a control point for tetrapy

5 amidotransferase converted Asp-tRNA(Asn) and Glu-tRNA(Gln) into Asn-tRNA and Gln-tRNA, respectively.

6 modify Asp-tRNA(Asn) into Asn-tRNA(Asn) and Glu-tRNA(Gln) into Gln-tRNA(Gln); (iv) the TonB receptor

7 t the enzyme transamidates Asp-tRNA(Asn) and Glu-tRNA(Gln) with similar efficiency (k(cat)/K(m) of 13

8 tion of ATP hydrolysis requires both Gln and Glu-tRNA(Gln).

9 ty capable of forming both Glu-tRNA(Glu) and Glu-tRNA(Gln).

10 red by the glutamine-dependent Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase (Asp/Glu-Adt).

11 minating GluRS (D-GluRS) that biosynthesizes Glu-tRNA(Glu) and cannot make Glu-tRNA(Gln).

12 xed tRNA specificity capable of forming both Glu-tRNA(Glu) and Glu-tRNA(Gln).

13 glutaminase but only in the presence of both Glu-tRNA(Gln) and the other subunit, GatE.

14 Glutamyl-queuosine, like canonical Glu-tRNA, was hydrolyzed by mild alkaline treatment.

15 In contrast, Glu-tRNA(Gln) stimulates basal ATP hydrolysis slightly,

16 se a specialized amidotransferase to convert Glu-tRNA(Gln) to Gln-tRNA(Gln) needed for protein synthe

17 drolyze glutamine and were unable to convert Glu-tRNA(Gln) to Gln-tRNA(Gln) when glutamine was the am

18 glutaminyl-tRNA amidotransferase to convert Glu-tRNA(Gln) to Gln-tRNA(Gln).

19 tRNA(Gln), and an amidotransferase converts Glu-tRNA(Gln) to Gln-tRNA(Gln).

20 demonstrated that recombinant GatAB converts Glu-tRNA(Gln) to Gln-tRNA(Gln) in vitro.

21 li, a bacterium that does not utilize either Glu-tRNA (Gln) or Asp-tRNA (Asn), and the second from He

22 t, GluRS2 only misacylates tRNA(Gln) to form Glu-tRNA(Gln).

23 n) is primarily synthesized by first forming Glu-tRNA(Gln), followed by conversion to Gln-tRNA(Gln) b

24 ng glutamyl-tRNA synthetase (ND-GluRS) forms Glu-tRNA(Gln), while the heterodimeric amidotransferase

25 Gln-tRNA(Gln) is synthesized from Glu-tRNA(Gln) in most microorganisms by a tRNA-dependent

26 However, Glu-tRNA(Gln) activates the glutaminase activity of the

27 n), the proposed high energy intermediate in Glu-tRNA(Gln) transamidation.

28 uring the misaminoacylated tRNA intermediate Glu-tRNA(Gln).

29 Based on its Glu-tRNA primer binding site specificity and the locatio

30 biosynthesizes Glu-tRNA(Glu) and cannot make Glu-tRNA(Gln).

31 etase produce Gln-tRNA(Gln) from misacylated Glu-tRNA(Gln) through the transamidation activity of Glu

32 the production of Gln-tRNA(Gln): misacylated Glu-tRNA(Gln) is transamidated by a Gln-dependent amidot

33 essential for the production of misacylated Glu-tRNA(Gln).

34 erichia coli GlnRS to synthesize misacylated Glu-tRNA(Gln) by 16,000-fold.

35 n-tRNA(Asn) by conversion of the misacylated Glu-tRNA(Gln) and Asp-tRNA(Asn) species catalyzed by the

36 hermautotrophicus that allows the mischarged Glu-tRNA(Gln) made by the tRNA synthetase to be channele

37 The activation of Glu-tRNA(Gln) via gamma-phosphorylation bears a similari

38 ln hydrolysis, ATP hydrolysis, activation of Glu-tRNA(Gln), and aminolysis of activated tRNA by Gln-d

39 (Gln) through the transamidation activity of Glu-tRNA(Gln) amidotransferase (Glu-AdT).

40 teria form Gln-tRNA(GLN) by the amidation of Glu-tRNA(GLN), only a few members of the gamma subdivisi

41 rophicus GluRS(ND), which is also capable of Glu-tRNA(Gln) synthesis, now shows that both k(cat) and

42 The formation of Glu-tRNA in mitochondria and the cytoplasm is catalyzed

43 dT is very low in the absence or presence of Glu-tRNA(Gln) and Gln.

44 The fact that only Glu-tRNA(Gln) but not tRNA(Gln) could activate the gluta

45 ransamidation of mischarged Asp-tRNA(Asn) or Glu-tRNA(Gln) catalyzed by a heterotrimeric amidotransfe

46 e formation of mis-acylated Asp-tRNA(Asn) or Glu-tRNA(Gln), and the subsequent amidation of these ami

47 rokaryotes requires amidation of Asp-tRNA or Glu-tRNA by amidotransferases that couple an amidase or

48 zed by-product derived from gamma-phosphoryl-Glu-tRNA(Gln), the proposed high energy intermediate in

49 nt amidation of the mischarged tRNA species, Glu-tRNA(Gln) or Asp-tRNA(Asn).

50 ating glutamyl-tRNA synthetase to synthesize Glu-tRNA(Gln) and a glutaminyl-tRNA amidotransferase to

51 gineered hybrid (GlnRS S1/L1/L2) synthesizes Glu-tRNA(Gln) more than 10(4)-fold more efficiently than

52 n-tRNA(Gln) 10(7)-fold more efficiently than Glu-tRNA(Gln) and requires tRNA to synthesize the activa

53 ylated tRNAs, confirming the prediction that Glu-tRNA (Gln), like Asp-tRNA (Asn), will not form a com

54 ociation), Gln-competitive inhibition of the Glu-tRNA(Gln)/ATP-independent glutaminase activity of Gl

55 ri or B. subtilis, and M. thermautotrophicus Glu-tRNA(Asn).

56 unable to transamidate M. thermautotrophicus Glu-tRNA(Gln).

57 l-tRNA synthetase from Thermus thermophilus (Glu-tRNA(Glu)) are considered.

58 suppression by Cys-tRNA(Pro), Ser-tRNA(Thr), Glu-tRNA(Gln), and Asp-tRNA(Asn).

59 3), using the latter product to transamidate Glu-tRNA(Gln) in concert with ATP hydrolysis.

60 rophicus GatCAB is capable of transamidating Glu-tRNA(Gln) from H. pylori or B. subtilis, and M. ther

61 pathway that relies on the misacylated tRNAs Glu-tRNA (Gln) and Asp-tRNA (Asn) as intermediates.

62 by His-tRNA(His) and, as also seen in vivo, Glu-tRNA(Glu).

63 all three activities waning in concert when Glu-tRNA(Gln) levels become exhausted.

64 activity by ATP or ATP-gammaS, together with Glu-tRNA(Gln), results either from an allosteric effect