ライフサイエンスコーパス: Golgi membrane

1 some but not all clathrin adaptors from the Golgi membrane.

2 tides enriched at the plasma membrane or the Golgi membrane.

3 fission step of COPI vesicle formation from Golgi membrane.

4 o known as giantin, that is localized at the Golgi membrane.

5 in is exposed to the cytoplasmic face of the Golgi membrane.

6 embling nucleotide sugar transporters of the Golgi membrane.

7 ite the fact that it was localized to the ER/Golgi membrane.

8 atidylinositol-4-phosphate (PI4P) within the Golgi membrane.

9 SLC35A3) form heterologous complexes in the Golgi membrane.

10 acetylglucosaminyltransferase (Mgat5) in the Golgi membrane.

11 250 kD and higher were detected in isolated Golgi membranes.

12 F that tethers COPI-coated vesicles to early Golgi membranes.

13 roteins required for centripetal motility of Golgi membranes.

14 phosphatidylserine analogue across purified Golgi membranes.

15 -depleted mitotic cytosol failed to fragment Golgi membranes.

16 required for COPI vesicle formation from the Golgi membranes.

17 tures during interphase and cosediments with Golgi membranes.

18 umulation of the palmitoylated form in trans-Golgi membranes.

19 ct of their depletion on organization of the Golgi membranes.

20 Ser262 is required for PITPbeta targeting to Golgi membranes.

21 nzyme chimeras remain stably associated with Golgi membranes.

22 tethering and fusion of COPII vesicles with Golgi membranes.

23 h the membrane trafficking activity of yeast Golgi membranes.

24 olgin family help to tether COPI vesicles to Golgi membranes.

25 arly Golgi, and accumulate both ER and early Golgi membranes.

26 tachment between coat protein I vesicles and Golgi membranes.

27 imitation, causing translocation of Sac1p to Golgi membranes.

28 disassembly, partitioning, and reassembly of Golgi membranes.

29 tdIns(4)P) at endoplasmic reticulum (ER) and Golgi membranes.

30 mechanism that directs vesicle tethering at Golgi membranes.

31 analysis to determine its residence time on Golgi membranes.

32 lular locations particularly associated with Golgi membranes.

33 Arf-GDP-Arf GEF complex, stabilizes GBF1 on Golgi membranes.

34 rotubule-nucleating ring complex proteins to Golgi membranes.

35 have established an association of Ras with Golgi membranes.

36 n indeed signal and be regulated in internal Golgi membranes.

37 The phosphorylated p47 does not bind to Golgi membranes.

38 sed the levels of lysophosphatidylcholine in Golgi membranes.

39 s Arf1 becomes inactive and dissociates from Golgi membranes.

40 e plasma membrane or the lysosomal and trans-Golgi membranes.

41 The tyrosine kinase Src is present on the Golgi membranes.

42 nstrated that GGAs 1 and 2 form a complex on Golgi membranes.

43 AREs, these vesicles are unable to fuse with Golgi membranes.

44 ed) during the dynein-dependent transport of Golgi membranes.

45 p115 tethers coat protein (COP)I vesicles to Golgi membranes.

46 lgins, Giantin on COPI vesicles, to GM130 on Golgi membranes.

47 is found both on the plasma membrane and on Golgi membranes.

48 ially regulating interactions of Galphai3 on Golgi membranes.

49 Pase activity, an enzyme marker specific for Golgi membranes.

50 structure disassembles, leaving no residual Golgi membranes.

51 of the toxin to the Golgi and for effects on Golgi membranes.

52 n signal that recruits importin alpha to the Golgi membranes.

53 osition, indicating a loose association with Golgi membranes.

54 S-palmitoylation to specifically localize to Golgi membranes.

55 thus disrupting the recruitment of dynein to Golgi membranes.

56 multiple Golgi markers and cosediments with Golgi membranes.

57 andem PDZ domains, is required to tether the Golgi membranes.

58 RHBDD2 is important for its packing into the Golgi membranes.

59 fect the localization of betaIII spectrin to Golgi membranes.

60 ort of selective secretory cargo at the late Golgi membranes.

61 f utrophin and DG from endoplasmic reticulum/Golgi membranes.

62 e Golgi protein golgin160 recruits dynein to Golgi membranes.

63 ucleus, provided that they are released from Golgi membranes after cleavage.

64 Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannos

65 rgeting of GAD65 to the cytosolic leaflet of Golgi membranes, an obligatory first step in axonal sort

66 brane in response to low oxygen requires the Golgi membrane-anchored Dsc E3 ligase complex.

67 did not tether karyopherin alpha 2 to the ER/Golgi membrane and allowed the import of the STAT1 compl

68 During this process, the Golgi membrane and clathrin seem to provide a scaffold t

69 We show that Syx1A is associated with the Golgi membrane and is required for the transportation of

70 roteins involved in Golgi trafficking to the Golgi membrane and promotes anterograde transport of sec

71 of FTCD to specifically interacts with both Golgi membrane and vimentin filaments and promote their

72 and Exo70 in HepG2 cells were visualized at Golgi membranes and apparently at the microtubule-organi

73 They are likely to originate from cis-Golgi membranes and are represented during the early sta

74 brane protein that localizes to endosomal or Golgi membranes and cofractionates with Tor1p.

75 n complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted i

76 in macromolecular complexes and move between Golgi membranes and cytosol.

77 These proteins colocalized to ER and Golgi membranes and exist in a detergent-soluble complex

78 sis revealed a transient interaction between Golgi membranes and GFP-p150(Glued)-labeled microtubules

79 Golgin-160 and PIST colocalize to Golgi membranes and interact in vivo.

80 Yos1p localizes to ER and Golgi membranes and is efficiently packaged into ER-deri

81 factor WASp homologue associated with actin Golgi membranes and microtubules (WHAMM), as well as the

82 ncies result in disorganization of mammalian Golgi membranes and mitotic defects characterized by mul

83 n mammals that involves organization of both Golgi membranes and mitotic spindles and that both enzym

84 s that GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force req

85 ithout affecting the overall organization of Golgi membranes and protein transport.

86 implicated in the recruitment of coatomer to Golgi membranes and release of nascent secretory vesicle

87 The precise location of Golgi membranes and resident proteins during mitosis rem

88 Abp1 are sufficient for regulated binding to Golgi membranes and subcellular localization.

89 ifications of the GAD65 protein target it to Golgi membranes and synaptic vesicles in neuroendocrine

90 and ER stress) are sensed and integrated at Golgi membranes and that golgin-160 plays an important r

91 haracterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.

92 ized in vitro with isolated maize coleoptile Golgi membranes and the nucleotide-sugar substrate, UDP-

93 ized in vitro with isolated maize (Zea mays) Golgi membranes and UDP-[(14)C]d-glucose.

94 ity gradients were used for fractionation of Golgi membranes and vesicles.

95 ose is consumed at the cytosolic side of the Golgi membrane, and the glucan product is extruded throu

96 ion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes n

97 rotein to the endoplasmic reticulum (ER), to Golgi membranes, and into SNARE complexes with Bet1p, Bo

98 Both alpha1 and alpha2 partially localize on Golgi membranes, and purified catalytically active, but

99 go rapid exchange between the cytosol and ER/Golgi membranes, and that wild-type GFP-HRas and GFP-NRa

100 t is likely that Yck2p first associates with Golgi membranes, and then is somehow recruited to buddin

101 Myosin VI is present in purified Golgi membranes as a peripheral membrane protein, target

102 or by elevated PP2A association with mitotic Golgi membranes as well as increased catalytic activity

103 ein functions in virion envelopment at trans-Golgi membranes, as well as in retrograde and anterograd

104 of alternative clearance pathways including Golgi membrane-associated and nucleophagy-based LaminB1

105 Golgi membrane-associated dynein was markedly decreased,

106 tallin (alphaB) is known as an intracellular Golgi membrane-associated small heat shock protein.

107 icrotubule-dependent process, and to depress Golgi membrane association of the GTP-conformer of rab6.

108 includes an N-terminal domain that mediates Golgi membrane association, a coiled-coil region that bi

109 Rab acceptor (mPRA1) is associated with the Golgi membrane at steady state and interacts with Rab pr

110 oluble factor is permitted to associate with Golgi membranes at synthesis-enhancing CHAPS concentrati

111 4-phosphate (PtdIns4P) has been localized to Golgi membranes based on the distribution of lipid bindi

112 CSLA9 and CSLC4 are found predominantly in Golgi membranes, based on co-localization with the known

113 In the context of biological Golgi membrane, both palmitoyl-coenzyme A (p-coA) and AR

114 purifying and expressing the low-abundance, Golgi membrane-bound pectin biosynthetic enzymes.

115 actor needed for COPI vesicle formation from Golgi membrane but also reveal a surprising mechanism by

116 accelerates protein trafficking through the Golgi membranes but also has striking negative effects o

117 ted fusion, dSyntaxin16 is not restricted to Golgi membranes but also present on lysosomes.

118 ease of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less effect on the organization

119 ar approach to rapidly deplete PtdIns4P from Golgi membranes by a recruitable Sac1 phosphatase enzyme

120 COPI in these mutants is released from Golgi membranes by brefeldin A, a drug that binds direct

121 GRASP55 stacks Golgi membranes by forming oligomers through its N-termi

122 otein could be diverted from this pathway to Golgi membranes by the addition of COPII-binding sites.

123 ene in mice to analyze the functions of this Golgi membrane Ca(2+) pump.

124 gi proteins for coordinating the behavior of Golgi membranes, chromosomes, and cytoskeleton during mi

125 Activated Rab6 on Golgi membranes colocalized with centrin during mitosis,

126 ted in the clathrin-depleted cells, and post-Golgi membrane compartments were swollen.

127 Mitotic Golgi membranes concentrate around the spindle poles, su

128 coat, which by assembling into a lattice on Golgi membranes concentrates cargo at specific sites and

129 ents showed that the complexes remain mobile Golgi membrane constituents that can relocate to the end

130 site directions across endoplasmic reticulum-Golgi membrane contact sites.

131 ing electron microscopy and demonstrate that Golgi membranes contain elements that are in close assoc

132 s containing the Bet1p derivative fused with Golgi membranes containing the Sec22p derivative.

133 and integrity by predominantly affecting the Golgi membrane/cytosol partitioning of ADP-ribosylation

134 e period, the labeled polysaccharides of the Golgi membrane diminished with subsequent transfer to th

135 hosphoprotein 3-dependent (GOLPH3-dependent) Golgi membrane dispersal process that drives the budding

136 whereas FlnA associates dynamically with the Golgi membranes during budding and trafficking of transp

137 ific acyltransferase, LPAAT3, contributes to Golgi membrane dynamics by suppressing tubule formation.

138 echanism of ubiquitin-mediated regulation of Golgi membrane dynamics during the cell cycle.

139 ation are coordinated via VCIP135 to control Golgi membrane dynamics in the cell cycle.

140 ytosolic and membrane proteins that regulate Golgi membrane dynamics in the cell cycle.

141 gral membrane GTPase that may be involved in Golgi membrane dynamics or vesicle trafficking.

142 culum (ER)-associated degradation (ERAD), ER/Golgi membrane dynamics, and DNA replication.

143 omotypic fusion of endoplasmic reticulum and Golgi membranes, endoplasmic reticulum-associated protei

144 berrant accumulation of immunogenic GAD65 in Golgi membranes facilitates inappropriate presentation t

145 d peripherally to the cytoplasmic surface of Golgi membranes, fail to secrete AcbA and, thus, produce

146 hatase indicated that PI(4)P was required on Golgi membranes for fusion with coat protein complex II

147 ic reticulum (RER) and vesicles present in a Golgi membrane fraction.

148 In vitro phosphorylation experiments using Golgi membrane fractions showed that 7B2 could be phosph

149 s have allowed the reproducible isolation of Golgi membranes from Arabidopsis (Arabidopsis thaliana)

150 KIF1C can protect Golgi membranes from fragmentation in cells lacking an i

151 Golgi membranes, from yeast to humans, are uniquely enri

152 GBF1, predominantly associated with cis-Golgi membranes, functions early in the secretory pathwa

153 have devised a procedure to address whether Golgi membranes fuse with the Endoplasmic Reticulum (ER)

154 shown to be crucial in the p97-p47-mediated Golgi membrane fusion events.

155 egulates VCIP135 deubiquitinase activity and Golgi membrane fusion in the cell cycle remains unknown.

156 me required for p97/p47-mediated postmitotic Golgi membrane fusion, is phosphorylated at multiple sit

157 ent SNARE disassembly that was essential for Golgi membrane fusion.

158 laments enhanced GRASP65 oligomerization and Golgi membrane fusion.

159 vate the enzyme and inhibit p97/p47-mediated Golgi membrane fusion.

160 mutant protein in cells impaired postmitotic Golgi membrane fusion.

161 embly is required for subsequent postmitotic Golgi membrane fusion.

162 polo-like kinases, which alone will unstack Golgi membranes, generating single cisternae.

163 s of proteins that bind to each other and to Golgi membranes have been implicated in the adhesion of

164 localized to the endoplasmic reticulum (ER)/Golgi membrane in infected cells, where it binds to and

165 xocytic pathway, and no H-Ras was visible on Golgi membranes in >80% of the cells.

166 binding proteins found previously to bind to Golgi membranes in an ARF1-dependent manner in vitro.

167 By electron microscopy, Golgi membranes in BIG1-depleted cells were less sharply

168 e soluble secretory proteins at the TGN/late Golgi membranes in eukaryotes.

169 for the cleavage of the noncompact zones of Golgi membranes in G2 phase.

170 e that cytosolic calnuc binds to Galphai3 on Golgi membranes in living cells and that Galphai3 must b

171 t sphingomyelin (SM) metabolism at the trans-Golgi membranes in mammalian cells essentially controls

172 with PIP2;7 at the endoplasmic reticulum and Golgi membranes in planta.

173 The fragmented and dispersed Golgi membranes in prometaphase and later stages of mito

174 ER)), which resulted in the fragmentation of Golgi membranes in response to CerS6/C(16)-ceramide alte

175 oordinating the secretory capacity of ER and Golgi membranes in response to growth conditions.

176 monstrate a role for rab8 in docking of post-Golgi membranes in rods, and constitute the first report

177 pose that Sff regulates vesicle tethering at Golgi membranes in the developing Drosophila embryo.

178 ubstantial amount of COPI is associated with Golgi membranes in the gea2-ts mutants, even after prolo

179 ed series, is required to produce fragmented Golgi membranes in the pericentriolar region that is cha

180 Human Hook3 bound to Golgi membranes in vitro and was enriched in the cis-Gol

181 esulted in the complete fragmentation of the Golgi membranes in vitro into 50- to 100-nm vesicles; th

182 sufficient to target a GFP-fusion protein to Golgi membranes in vivo.

183 elective recruitment of mAbp1 and drebrin to Golgi membranes indicate how actin-based structures are

184 Partitioning of the Golgi membrane into daughter cells during mammalian cell

185 , but not the inactive mutant, converted the Golgi membranes into COPI vesicles.

186 ith a putative function in redistribution of Golgi membranes into the endoplasmic reticulum in Drosop

187 hat depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletio

188 ctional organization of these enzymes in the Golgi membrane is currently unknown.

189 Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesi

190 recruitment of Arf GEFs, and hence Arfs, to Golgi membranes is not understood.

191 Similar accumulation of GAD65 in Golgi membranes is observed in human beta-cells in pancr

192 Like ARFs and ARL1, the binding of SCOCO to Golgi membranes is rapidly reversed by brefeldin A, sugg

193 lmitoyl transferase for Yck2p, is located on Golgi membranes, it is likely that Yck2p first associate

194 er is synthesized on the cytoplasmic face of Golgi membranes, it must be flipped to the non-cytoplasm

195 erase (LPAT) induces the rapid tubulation of Golgi membranes, leading in their retrograde movement to

196 Retention of import factors at the ER/Golgi membrane leads to a loss of STAT1 transport into t

197 In addition, Golgi membrane lipid order disruption byd-ceramide-C6 ca

198 ith wild-type Lck, W97ALck displays aberrant Golgi membrane localization.

199 p the functional domains required for proper Golgi membrane localization.

200 Fragmented Golgi membranes maintained their juxtanuclear localizati

201 small fraction of RabA4b cofractionated with Golgi membrane marker proteins, the majority of this pro

202 s thaliana mutant that partially accumulates Golgi membrane markers and a soluble secretory marker in

203 ng photobleaching techniques, we showed that Golgi membrane markers constitutively cycle to and from

204 d the colocalization of adiponectin with the Golgi membrane markers p115, beta-COP, and the trans-Gol

205 ng the endoplasmic reticulum (ER) toward the Golgi membranes mimics the effects of depleting PI(4)P a

206 fection, the two proteins are colocalized in Golgi membranes, near the coronavirus budding site.

207 ccumulation of pro-ATF-6 in the disrupted ER/Golgi membrane network, where pro-ATF6 is activated.

208 Here, we formed ER and Golgi membrane networks in vitro and used optical tweeze

209 l membrane during entry and across the trans-Golgi membrane of infected cells during viral maturation

210 fluorescently labelled coatomer and Arf1 on Golgi membranes of living cells.

211 Golgi membranes of Spca1(-/-) embryos were dilated, had

212 he major phosphorylation target in rat liver Golgi membranes of two mitotic kinases, cdc2-cyclin B an

213 mbranes of vesicles and colocalizes with the Golgi membrane or early endosome membrane.

214 cell wall polysaccharides are synthesized in Golgi membranes, packaged into vesicles, and exported to

215 The assembly did not require detectable Golgi membranes, preexisting VTCs, or COPI function.

216 fractions (characterized by the presence of Golgi membrane protein GM130 and vesicle coat protein ga

217 GP73 is a novel type II Golgi membrane protein of unknown function that is expre

218 ath via down-regulation of GPP130, a cycling Golgi membrane protein that serves as an endosome-to-Gol

219 of their cytosolic domains by the peripheral Golgi membrane protein Vps74, an orthologue of human GOL

220 or two of the seven Cog1- or Cog2-dependent Golgi membrane proteins called GEARs are also sensitive

221 pendent sorting mechanisms target aggregated Golgi membrane proteins for lysosomal degradation.

222 ocalization of the plasma membrane proteins, Golgi membrane proteins Kex2 and Vrg4 are also missorted

223 ptotic proteins (GAAPs) are highly conserved Golgi membrane proteins that inhibit apoptosis and promo

224 hydrolases, vacuolar membrane proteins, and Golgi membrane proteins unable to recycle back to the Go

225 tribution without changing that of intrinsic Golgi membrane proteins.

226 Asap may recycle Arf1 to the Golgi from post-Golgi membranes, providing optimal Golgi output for spec

227 The isolated cPLA2 C2 domain associated with Golgi membranes rapidly in proportion to the [Ca2+]i, al

228 effectively disperses the ER, the ERGIC, and Golgi membranes, redistributed N with the ERGIC, implica

229 PI4KIIIbeta recruitment to Golgi membranes relies on GBF1/Arf and ACBD3.

230 Based on these results, we conclude that Golgi membranes remain separate from the ER during mitos

231 nism and regulation of dynein recruitment to Golgi membranes remains unknown.

232 The role of lipid metabolic enzymes in Golgi membrane remodeling is a subject of intense intere

233 oreover, whether COPI vesicle formation from Golgi membrane requires additional factors also remains

234 Budding of COPI-coated vesicles from Golgi membranes requires an Arf family G protein and the

235 apparatus, where constant inward movement of Golgi membranes results in its characteristic position n

236 ifferent sphingolipid content, pre- and post-Golgi membranes serve different cellular functions.

237 activities may help to coordinately regulate Golgi membrane shape and tubule formation.

238 Coarse-grained simulations of the complex Golgi membrane suggest the N-terminal domain may induce

239 ma membrane, whereas others are recruited to Golgi membranes, suggesting that exocyst assembly tether

240 vesicles but not in endoplasmic reticulum or Golgi membranes, suggesting that the effects of FE65L1 o

241 The endoplasmic reticulum (ER) and Golgi membrane system have major roles in cell signaling

242 h nucleus becoming surrounded by a single ER/Golgi membrane system separate from adjacent ones.

243 s its self-association, leading to a loss of Golgi membrane tethering, cisternal unlinking, and Golgi

244 is formed and has a longer residence time on Golgi membranes than GBF1 or Arf1 alone.

245 that the central portion of TMF can bind to Golgi membranes that are liberated of their COPI cover.

246 PRA1 is a 21-kDa protein associated with Golgi membranes that binds to prenylated Rab proteins in

247 nt on both donor COPII vesicles and acceptor Golgi membranes, the formation of disulfide cross-links

248 ases are known to exist as homodimers in the Golgi membranes, this organization level may represent o

249 ng ubiquitin ligase HACE1 is targeted to the Golgi membrane through interactions with Rab proteins.

250 Mena is recruited onto the Golgi membranes through interaction with GRASP65.

251 sitively regulate Drs2p activity in isolated Golgi membranes through interaction with the C-terminal

252 anism that does not involve the recycling of Golgi membranes through the ER.

253 H3 does so through its ability to link trans-Golgi membranes to F-actin via its interaction with myos

254 Activity was purified from Golgi membranes to greater than 220-fold by extraction w

255 s been developed using purified proteins and Golgi membranes to reconstitute the Golgi disassembly an

256 al plasma membrane and traffic directly from Golgi membranes to the canalicular membrane.

257 s, which entails subunitB2translocation from Golgi membranes to the cytosol.

258 are further captured by GM130, thus linking Golgi membranes to the spindle.

259 Golgi vesicular trafficking, thus remodeling Golgi membrane traffic and redirecting Golgi-derived ves

260 However, the role of ArfGAPs in post-Golgi membrane traffic has not been defined.

261 mutations in YKT6 specifically affects post-Golgi membrane traffic to the vacuole, and the effects o

262 bind to clathrin adaptors, function in post Golgi membrane traffic, and have been implicated in glio

263 Here, we demonstrate that post-Golgi membrane trafficking is polarized toward longer de

264 ), a SNARE involved in endoplasmic reticulum/Golgi membrane trafficking.

265 es Arl1 and Arl8, suggesting a role in trans-Golgi membrane trafficking.

266 protein with unusual localization on ER and Golgi membranes, translocates in response to amino acid

267 Surprisingly, inhibition of Golgi membrane transport with brefeldin A did not preven

268 These results suggest that Golgi membrane tubule formation can result from increasi

269 e, but not inactive alpha1 and alpha2 induce Golgi membrane tubule formation in a reconstitution syst

270 fic acyltransferase, LPAAT3, which regulates Golgi membrane tubule formation, trafficking, and struct

271 AT3 significantly inhibited the formation of Golgi membrane tubules in vivo and in vitro.

272 ent studies have suggested that formation of Golgi membrane tubules involves the generation of membra

273 cling, and show that retrograde transport of Golgi membrane underlies Golgi dispersal during microtub

274 his oxysterol selectively accumulates in the Golgi membrane using a pathway that is sensitive to ATP

275 teins associated with interphase and mitotic Golgi membranes using a proteomic approach.

276 KD is known to be involved in the control of Golgi membrane vesicular and lipid transport, we hypothe

277 ng was observed when TyA-GFP was targeted to Golgi membranes via a phosphatidylinositol 4-phosphate-b

278 must be anchored to the cytosolic surface of Golgi membranes via lipid anchors for the interaction to

279 l to binding Golgi stacks to condensation of Golgi membranes was found to correlate with the time and

280 from the ER, but their docking or fusion to Golgi membranes was inhibited.

281 of purified kinases, ARF1 and coatomer, the Golgi membranes were completely fragmented into vesicles

282 Isolated rat liver Golgi membranes were incubated with [(3)H]CMP sialic aci

283 tage were pulse labeled with [(14)C]O(2) and Golgi membranes were isolated from elongating cells at t

284 ciated with lipid rafts, whereas that in the Golgi membrane, where MHV matures, was not.

285 s localized to the endoplasmic reticulum and Golgi membrane, where the ethylene receptors and the eth

286 idylinositol 4-phosphate (PtdIns(4)P) in the Golgi membrane, whereas its C-terminal StAR-related lipi

287 ere primarily associated with Golgi and post-Golgi membranes, whereas the dominant negative protein w

288 em to drive the ectopic synthesis of PIP2 on Golgi membranes, which normally have active Cdc42 but la

289 that BACE1 is largely situated in the distal Golgi membrane with a minor presence in the endoplasmic

290 the formation of COPI vesicles by incubating Golgi membrane with purified soluble components, and fin

291 f cisternae-specific tethering, we generated Golgi membranes with a single GRASP on all cisternae.

292 or expression of its GTP-locked form, intact Golgi membranes with bound peripheral proteins persist t

293 rotein occurs only upon artificial fusion of Golgi membranes with ER.

294 ts movement is spatially restricted to trans-Golgi membranes with limited lateral mobility.

295 ocess has been mimicked in vitro by treating Golgi membranes with mitotic and interphase cytosol.

296 Treatment of Golgi membranes with mitotic cytosol or with purified co

297 Incubation of purified rat liver Golgi membranes with mitotic HeLa cell cytosol led to fr

298 Treatment of Golgi membranes with mitotic kinases and COPI coat prote

299 Preloading Golgi membranes with short chain phosphatidic acid abrog

300 sidase component guarantees docking into the Golgi membrane, with the tags exposed in the lumen.