ライフサイエンスコーパス: Golgi stack

1 ies that it is used for transport across the Golgi stack.

2 gi intermediates and not to membranes of the Golgi stack.

3 pre-Golgi VTCs from peripheral sites to the Golgi stack.

4 embrane flow and cargo transport through the Golgi stack.

5 chimeric proteins were polarized within the Golgi stack.

6 uctures in recycling of Golgi enzymes in the Golgi stack.

7 nd have distinctive distributions within the Golgi stack.

8 atitis virus glycoprotein from the ER to the Golgi stack.

9 ns may modulate the flow patterns within the Golgi stack.

10 from endoplasmic reticulum exit sites to the Golgi stack.

11 e Toxoplasma gondii, which has only a single Golgi stack.

12 al proteins, RNA, and apolipoproteins in the Golgi stacks.

13 eak leads to a precocious formation of large Golgi stacks.

14 h the elongation itself and the formation of Golgi stacks.

15 dicated that the fusion proteins located all Golgi stacks.

16 to function in the postmitotic reassembly of Golgi stacks.

17 ganization of the pericentriolarly localized Golgi stacks.

18 axin 5 and membrin penetrate deeply into the Golgi stacks.

19 ialyl transferase to GFP was targeted to the Golgi stacks.

20 he absence of IQ is sufficient to vesiculate Golgi stacks.

21 abolite, causes complete vesiculation of the Golgi stacks.

22 on of the chimeric proteins occurred between Golgi stacks.

23 endoplasmic reticulum and fails to enter the Golgi stacks.

24 o transport vesicles at the terminal rims of Golgi stacks.

25 r sucrose transporter SUC4 is blocked in cis-Golgi stacks.

26 e reassembly of the Golgi fragments into new Golgi stacks.

27 eads to reassembly of the membranes into new Golgi stacks.

28 the daughter cells and reassembled into new Golgi stacks.

29 s and large skein-like structures entangling Golgi stacks.

30 r cells, where they are reassembled into new Golgi stacks.

31 ding plastids, mitochondria, peroxisomes and Golgi stacks.

32 sec16 mutant is accompanied by disruption of Golgi stacks.

33 omologue was isolated and shown to target to Golgi stacks.

34 f ST6GalNAc-I, which is found throughout the Golgi stacks.

35 the proportion of GRIP-GFP fusion protein on Golgi stacks.

36 in-13A and GhKinesin-13A localized to entire Golgi stacks.

37 the lateral edges of, and often connecting, Golgi stacks.

38 are needed in combination for GRASP-mediated Golgi stacking.

39 ns and provide insight into the mechanism of Golgi stacking.

40 th antibodies to GRASP65 fail to form proper Golgi stacks after cell division.

41 During metaphase, approximately 20% of all Golgi stacks aggregate in the immediate vicinity of the

42 5 and GRASP55, two proteins localized to the Golgi stack and early secretory pathway, mediate process

43 Golgi stack and peroxisome movements were also significa

44 e more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi networ

45 izing agents promote the alkalization of the Golgi stack and thetrans-Golgi network.

46 al micrometers) emanate from elements of the Golgi stack and trans Golgi network (TGN).

47 rganelles of the host cell (lysosomes, trans-Golgi stack and trans-Golgi network, and endoplasmic ret

48 ein 65 (GRASP65) has been implicated in both Golgi stacking and ribbon linking by forming trans-oligo

49 phy and immunolabeling techniques to examine Golgi stacks and associated vesicles in the cells of the

50 Ultrastructural analysis revealed enlarged Golgi stacks and increased transitional vesicles in vent

51 Reclustering of nocodazole-dispersed Golgi stacks and microtubule/dynein-dependent ER-to-Golg

52 e find the Golgi protein in the newly formed Golgi stacks and not in the ER.

53 t TGN cisternae apparently peel off from the Golgi stacks and persist for some time in the cytosol, a

54 Detergent-extracted Golgi stacks and TGN-containing membranes are closely as

55 es that these spots correspond to individual Golgi stacks and that the fusion protein is largely conf

56 lgi with lobe A preferential localization on Golgi stacks and the presence of lobe B on vesicle-like

57 e implicated in the architecture of both the Golgi stacks and the tER sites.

58 lgi marker indicates that RabA2 localizes in Golgi stacks and the trans-Golgi network.

59 ve cell homogenate) and by appearance of the Golgi stacks and vesicles on electron microscopy.

60 secretory cargoes could be restricted at the Golgi stack, and the entry of the trans-Golgi network in

61 he adjacent Golgi networks compared with the Golgi stack, and this enrichment varies with cell type.

62 yeasts: Pichia pastoris, which has coherent Golgi stacks, and Saccharomyces cerevisiae, which has a

63 detected in WIGs, the endoplasmic reticulum, Golgi stacks, and the trans-Golgi network in the Mn1 BET

64 maintenance of the interconnected ribbons of Golgi stacks, and tubule formation from endosomes.

65 ructural and enzymatic components of the new Golgi stack are laid down first, followed by those neede

66 It is thought that residents of the Golgi stack are localized by a retention mechanism that

67 sent evidence for a third model in which the Golgi stacks are a continuous structure and proteins rap

68 In P. pastoris, Golgi stacks are adjacent to discrete tER sites that con

69 We find that tER sites and the adjacent Golgi stacks are enclosed in a ribosome-excluding "matri

70 The pericentriolar Golgi stacks are fragmented and found dispersed in mitot

71 nzymes reside; in mammalian cells, dozens of Golgi stacks are often laterally linked into a ribbon-li

72 Golgi stacks are often located near sites of "transition

73 However, the scattered Golgi stacks are polarized and transport cargo.

74 nesis is consistent with the hypothesis that Golgi stacks are repositioned to ensure equal partitioni

75 velopment, in fine-tuning the positioning of Golgi stacks, as well as their involvement in cellulose

76 tory proteins, the overriding principle of a Golgi stack assembly is likely to be quite simple.

77 The ecdysone-triggered biogenesis of Golgi stacks at the onset of imaginal disc elongation of

78 Regeneration of Golgi stacks at these peripheral sites would re-establis

79 green fluorescent protein (GFP), locates to Golgi stacks but does not exactly co-locate with the Gol

80 t each MT population is capable of gathering Golgi stacks but not of establishing Golgi complex integ

81 cking the golgin TRIP11/GMAP-210 have normal Golgi stacks, but show developmental problems related to

82 GRASP55, Golgi stack length is shortened but Golgi stacking, compartmentalization, and transport seem

83 r wide area (FRAP-W) experiments, peripheral Golgi stacks continuously exchanged resident proteins wi

84 les and Kinesin-13A, and the organization of Golgi stacks could play a regulatory role in trichome mo

85 inesis, the daughter cells have very similar Golgi stack densities.

86 We have followed the redistribution of Golgi stacks during mitosis and cytokinesis in living to

87 The redistribution of Golgi stacks during mitosis and cytokinesis is consisten

88 o be phosphorylated, allows the cell to keep Golgi stacks during mitosis and has no effect on the equ

89 Golgi in HeLa cells, we stably expressed the Golgi stack enzyme N-acetylgalactosaminyltransferase-2 (

90 ate glycosylation, with the focus on how the Golgi stacking factors GRASP55 and GRASP65 generate the

91 posited within the cis-most cisternae of the Golgi stack following a 15 degrees C block.

92 In vitro studies have suggested that Golgi stack formation involves two homologous peripheral

93 shown that GRASP depletion in cells disrupts Golgi stack formation.

94 ules and Golgi stacks indicated an increased Golgi stack frequency at the preprophase band site.

95 pic event is accompanied by the formation of Golgi stacks from small Golgi larval clusters of vesicle

96 In mammalian cells, individual Golgi stacks fuse laterally to form the characteristic p

97 cretory pathway, mediate processes including Golgi stacking, Golgi ribbon linking and unconventional

98 insect cells that naturally harbor dispersed Golgi stacks have limited capacity to transport artifici

99 nonphosphorylatable GRASP55 mutants enhances Golgi stacking in interphase cells and inhibits Golgi di

100 event the formation of membrane tubules from Golgi stacks in an in vitro reconstitution system.

101 Golgi stacks in border cells and peripheral cells, precu

102 confirmed using brefeldin A to collapse the Golgi stacks in both HEK 293 and COS-1 cells.

103 kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells.

104 Golgi stacks in the border cells have hypertrophied marg

105 ms in the nucleated karyoplasts, whereas the Golgi stacks in the cytoplasts are scattered.

106 The Golgi stacks in these GRASP-deficient HeLa cells were no

107 We show now that Golgi stacks in vitro bind dynein supplied from cytosol

108 Golgi stacks, in permeabilized interphase normal rat kid

109 ving BY-2 cells labeled for microtubules and Golgi stacks indicated an increased Golgi stack frequenc

110 in hand with the compartmentalization of the Golgi stack into cis-, medial-, and trans-cisternae, whi

111 ble knockout of GRASP proteins disperses the Golgi stack into single cisternae and tubulovesicular st

112 zed homotypic membrane tethers that organize Golgi stacks into a long, contiguous ribbon-like structu

113 with brefeldin A or if GRASP65, which links Golgi stacks into a ribbon network, was depleted.

114 emains puzzling how GRASP65 physically links Golgi stacks into a ribbon.

115 xpressing NSF(E329Q) promotes disassembly of Golgi stacks into dispersed vesicular structures.

116 Mitotic conversion of Golgi stacks into mitotic clusters had surprisingly litt

117 A single Golgi stack is duplicated and partitioned into two daugh

118 ay ensure that the pool of UDP-GlcNAc in the Golgi stack is not depleted, thereby maintaining proper

119 ence of GRASP65, the number of cisternae per Golgi stack is reduced without affecting the overall org

120 These results indicate that although Golgi stacking is a highly complicated process involving

121 motor myosin-I and dynein, whereas isolated Golgi stacks lack dynein but contain myosin-I.

122 In the absence of GRASP55, Golgi stack length is shortened but Golgi stacking, comp

123 The sites of preferential Golgi stack localization are specific for this organelle

124 How does the Golgi stack mediate transport of cargo from the endoplas

125 s, illustrating how the most ancient form of Golgi stacking might have occurred using only weak ciste

126 We further report here that movement of Golgi stacks, mitochondria, and peroxisomes in the leaf

127 s revealed independent movement patterns for Golgi stacks, mitochondria, and peroxisomes, indicating

128 osin XI-K has a major role in trafficking of Golgi stacks, mitochondria, and peroxisomes, whereas myo

129 The Golgi stack, monitored using sialyltransferase, galactos

130 ed to important morphological changes in the Golgi stack morphology and the transitional ER (tER) org

131 ng ERES, and that an ERES and its associated Golgi stack move as a single secretory unit.

132 dding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic r

133 olling the transport of proteins through the Golgi stack of mammalian and plant cells is the subject

134 Likewise, reformation of Golgi stacks on removal of BFA was not dependent on eith

135 e elements could be positively identified as Golgi stacks or cisternae.

136 of these myosins also reduced trafficking of Golgi stacks, peroxisomes, and mitochondria in root hair

137 ndant and additive roles in the transport of Golgi stacks, peroxisomes, and mitochondria.

138 nases, cdc2 and plk, which phosphorylate the Golgi stacking protein GRASP65 and thus disrupt the olig

139 d was also observed for ERGIC-53 but not for Golgi stack proteins.

140 olgi cisternae requires dephosphorylation of Golgi stacking proteins by the protein phosphatase PP2A.

141 kDa (GRASP55) were originally identified as Golgi stacking proteins; however, subsequent GRASP knock

142 les to the nucleus while G3 continues to the Golgi stacks, providing passage for the entire core prot

143 During telophase and cytokinesis, many Golgi stacks redistribute around the phragmoplast where

144 cks stops, about one-third of the peripheral Golgi stacks redistributes to the perinuclear cytoplasm,

145 o untreated cells, suggesting that polarized Golgi stacks reform rapidly at scattered sites.

146 STB increases the rate of MT-dependent Golgi stack repositioning after nocodazole treatment.

147 he endoplasmic reticulum (ER) and within the Golgi stack, sorting transmembrane proteins bearing C-te

148 cell division, when the general streaming of Golgi stacks stops, about one-third of the peripheral Go

149 e for a countercurrent fusion pattern in the Golgi stack, the gradients involved would be strongly sh

150 m (ER) but does not disrupt the formation of Golgi stacks, the distribution of beta-COP, or the trans

151 hase, there are many scattered, disconnected Golgi stacks throughout the cytoplasm, which appear as 1

152 on that it is broadly distributed across the Golgi stack to serve the many sialyltransferases involve

153 LTA(1) localization from cytosol to binding Golgi stacks to condensation of Golgi membranes was foun

154 a method for inducing plant cells and their Golgi stacks to differentiate in a synchronous manner ha

155 luorescent protein fusion redistributes from Golgi stacks to the perinuclear region, where poliovirus

156 In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tono

157 ls consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that

158 that this ER-rich peripheral region excludes Golgi stacks, vacuoles, and amyloplasts but not mitochon

159 quent movement of ER-derived carriers to the Golgi stack was blocked by a trans-dominant ARF1 mutant

160 ntermediates with cisternal membranes of the Golgi stack was not observed under these conditions.

161 Aggregation/clustering of Golgi stacks was often observed in the kinesin-13a mutan

162 ivity of Plk3; Plk3-induced fragmentation of Golgi stacks was significantly reduced after treatment w

163 res and macromolecular compositions of these Golgi stacks, we examined high-pressure frozen/freeze-su

164 immunofluorescence microscopy, we show that Golgi stacks were converted into clusters of vesicles an

165 In UL133-UL138(NULL) virus-infected ECs, Golgi stacks were disrupted, and the viral assembly comp

166 wild-type and kinesin-13a mutant cells, the Golgi stacks were frequently associated with microtubule

167 n effect on the tER organization, though the Golgi stacks were greatly vesiculated in the cells deple

168 istributed to peripheral ER exit sites where Golgi stacks were regenerated.

169 Notably, in the absence of functional GNL1, Golgi stacks were rendered sensitive to the selective AR

170 The Golgi stacks were shown to move rapidly and extensively

171 by siRNA reduces the number of cisternae per Golgi stack, whereas simultaneous knockdown of both GRAS

172 e tER sites and therefore generates coherent Golgi stacks, whereas S. cerevisiae has a delocalized tE

173 RAB11A, SNAP23, and CDC42 caused the loss of Golgi stacks with reorganization into structures that re

174 rom oligosaccharide-modifying enzymes in the Golgi stack without affecting their ability to form a ri