ライフサイエンスコーパス: Golgin

1 hagy, demonstrating an unexpected role for a golgin.

2 olecular weight coiled-coil proteins, termed golgins.

3 been classified as a family of proteins, the golgins [1].

4 These include GM130, golgin 160, and the Golgi vesicle tethering protein p115

5 dominantly prevented cleavage of endogenous golgin-160 after ligation of death receptors or inductio

6 Here, we identify golgin-160 and GMAP210 as proteins required for centripe

7 ese findings not only reveal novel roles for golgin-160 and GMAP210 in conferring membrane motility b

8 Full-length golgin-160 and golgin-160B may link distinct subsets of

9 Golgin-160 and PIST colocalize to Golgi membranes and in

10 rminus binds a GSV anchoring site comprising Golgin-160 and possibly other proteins.

11 some pro-apoptotic stimuli; thus cleavage of golgin-160 appears to play a role in apoptotic signaling

12 Using the N-terminal head domain of golgin-160 as bait in a yeast two-hybrid screen, the pos

13 lized to the Golgi complex, where it cleaves golgin-160 at a unique site not susceptible to cleavage

14 Golgin-160 belongs to the golgin family of Golgi-localiz

15 P60 interacts preferentially with one of the golgin-160 caspase cleavage fragments (residues 140-311)

16 s rescued by expression of a siRNA-resistant golgin-160 cDNA.

17 ptosis in cells expressing caspase-resistant golgin-160 could not be bypassed by expression of potent

18 The caspase-resistant golgin-160 dominantly prevented cleavage of endogenous g

19 itro and disrupted the ability to retain the golgin-160 fragment at the Golgi in cells.

20 This strong interaction prevented the golgin-160 fragment from accumulating in the nucleus whe

21 raction increases the Golgi retention of the golgin-160 fragment in cells overexpressing GCP60.

22 nteraction with one of the caspase-generated golgin-160 fragments (residues 140-311).

23 ear translocation and potential functions of golgin-160 fragments.

24 Golgin-160 has a large coiled-coil C-terminal domain and

25 ybrid assay to screen for interactors of the golgin-160 head and identified GCP60 (Golgi complex-asso

26 event regulated not only by cleavage of the golgin-160 head but also by the oxidation state of GCP60

27 resident protein, interacts weakly with the golgin-160 head domain but has a strong interaction with

28 Caspase cleavage of the golgin-160 head domain generates different fragments tha

29 Because the golgin-160 head is cleaved by caspases during apoptosis,

30 Expression of a non-cleavable form of golgin-160 impairs apoptosis induced by some pro-apoptot

31 asma membrane proteins, supporting a role of golgin-160 in vesicular trafficking.

32 Golgin-160 is a coiled-coil protein on the cytoplasmic f

33 Golgin-160 is a member of the golgin family of Golgi-loc

34 The peripheral Golgi protein golgin-160 is induced during 3T3L1 adipogenesis and is p

35 The COOH-terminal two-thirds of golgin-160 is predicted to form a coiled-coil, with an N

36 Golgin-160 is ubiquitously expressed in vertebrates.

37 Therefore, our data suggest that golgin-160 may participate in PIST-dependent trafficking

38 ents identified a leucine-rich repeat within golgin-160 necessary for interaction with PIST.

39 Together, these data demonstrate that golgin-160 plays an important role in directing insulin-

40 d and integrated at Golgi membranes and that golgin-160 plays an important role in transduction of th

41 Golgin-160 possesses an N-terminal non-coiled-coil "head

42 nterfering RNA (siRNA)-mediated reduction in golgin-160 protein resulted in an increase accumulation

43 information is present in the same region of golgin-160 suggests that this protein may have more than

44 ntact TUG links GLUT4 to PIST and also binds Golgin-160 through its C-terminal region.

45 ly expressing wild-type or caspase-resistant golgin-160 to several proapoptotic stimuli.

46 itive; in its reduced form, interaction with golgin-160 was diminished or abolished, whereas oxidatio

47 ing to the plasma membrane in the absence of golgin-160 was independent of TGN/Golgi sorting, because

48 lls expressing a caspase-resistant mutant of golgin-160 were strikingly resistant to apoptosis induce

49 Golgin-160, a ubiquitous protein in vertebrates, localiz

50 identify the Golgi targeting information in golgin-160, full-length and deletion constructs tagged w

51 lso discovered a widely expressed isoform of golgin-160, golgin-160B, which lacks the exon encoding t

52 n of potential caspase cleavage fragments of golgin-160, or by drug-induced disassembly of the Golgi

53 ndings suggest that nuclear translocation of golgin-160-(140-311) is a highly coordinated event regul

54 rosporine, suggesting that nuclear-localized golgin-160-(140-311) might promote cell survival.

55 Here we studied the interaction of golgin-160-(140-311) with GCP60 and identified a single

56 The TUG C-terminal peptide bound the Golgin-160-associated protein, ACBD3 (acyl-CoA-binding d

57 d-coil domain, which interacts directly with golgin-160.

58 x in the absence of assembly with endogenous golgin-160.

59 dies against Golgin-95/GM130, Golgin-97, and Golgin-160.

60 otein related to the mammalian Golgi protein golgin-160.

61 n reduced binding of TUG to ACBD3 but not to Golgin-160.

62 TC10 (PIST) was identified to interact with golgin-160.

63 gin-230/245/256, golgin-97, GM130/golgin-95, golgin-160/MEA-2/GCP170, giantin/macrogolgin and a relat

64 Full-length golgin-160 and golgin-160B may link distinct subsets of proteins to eff

65 As predicted, golgin-160B was unable to bind PIST.

66 ed a widely expressed isoform of golgin-160, golgin-160B, which lacks the exon encoding the leucine r

67 lgi apparatus of two members of this family, golgin-230/245/256 and golgin-97, was investigated.

68 This family includes golgin-230/245/256, golgin-97, GM130/golgin-95, golgin-1

69 een ARL1 and two binding proteins, SCOCO and Golgin-245, are defined and characterized in more detail

70 trixlike tail region that sometimes contains Golgin-245.

71 These findings suggest that Golgin-45 serves as a linchpin for the maintenance of Go

72 al. report the discovery of a protein named Golgin-45 that is located on the surface of the middle (

73 oteins and report here the identification of golgin-84 as a novel mitotic target.

74 Golgin-84 binds to active rab1 but not cis-Golgi matrix

75 t showed that the N-terminal 497 residues of golgin-84 contain a coiled-coil domain that when fused t

76 the volume of a normal Golgi apparatus upon golgin-84 depletion.

77 Cross-linking indicates that golgin-84 forms dimers, consistent with the prediction o

78 Golgin-84 is an integral membrane protein with a single

79 Data base searching also indicates golgin-84 is similar in structure and sequence to gianti

80 Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport

81 Our results suggest that golgin-84 plays a key role in the assembly and maintenan

82 Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon.

83 ng cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is

84 II, GOS-28, GS15, GPP130, CASP, giantin, and golgin-84) whose abundances were reduced in the mutant c

85 e best studied, and here we characterize the golgin-84-CASP tether.

86 approximately 84-kDa Golgi protein we termed golgin-84.

87 ncludes golgin-230/245/256, golgin-97, GM130/golgin-95, golgin-160/MEA-2/GCP170, giantin/macrogolgin

88 irmed by using polyclonal antibodies against Golgin-95/GM130, Golgin-97, and Golgin-160.

89 hermore, by proteomic analysis we identified Golgin-97 as a FIP1/RCP-binding protein.

90 Binding of FIP1/RCP to Golgin-97 does not affect Golgin-97 recruitment to the T

91 discuss the potential role and functions of golgin-97 in poxvirus replication and propose two workin

92 Inside the virion, golgin-97 is associated with the insoluble core protein

93 Thus, we propose that FIP1/RCP binding to Golgin-97 is required for tethering and fusion of recycl

94 ing of FIP1/RCP to Golgin-97 does not affect Golgin-97 recruitment to the TGN, but appears to regulat

95 lular localization of the trans-Golgi marker Golgin-97 suggested differences in the organization of t

96 RCP-binding domain maps to the C-terminus of Golgin-97, adjacent to its GRIP domain.

97 lyclonal antibodies against Golgin-95/GM130, Golgin-97, and Golgin-160.

98 gi complex with specific markers, anti-human Golgin-97, anti-KDEL receptor, and BODIPY-TR ceramide, s

99 This family includes golgin-230/245/256, golgin-97, GM130/golgin-95, golgin-160/MEA-2/GCP170, gia

100 esiding in the trans-Golgi network membrane, golgin-97, is transported to the sites of virus replicat

101 mbers of this family, golgin-230/245/256 and golgin-97, was investigated.

102 ing only partial overlap with the TGN marker golgin-97.

103 These findings demonstrate that golgins act as tethers in vivo, and hence the specificit

104 e the distance between the distal end of the golgin and the target membrane thereby promoting tighter

105 whether its mechanism of action requires its golgin and/or SNARE interactions.

106 f COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).

107 Golgins are extended coiled-coil proteins believed to pa

108 Golgins are Golgi-localized proteins present in all mole

109 malian cells more emphasis has been given to golgins as a potentially stable assembly framework.

110 Moreover, although golgins bind to Rab GTPases, the functional significance

111 115 lacking its phosphorylation site and the golgin-binding domains also restored the Golgi apparatus

112 -tethering activities of 10 widely conserved golgin coiled-coil proteins.

113 ltransferases and glycosidases into nascent, golgin-enriched structures after drug washout.

114 hout in HeLa cells, we found that scattered, golgin-enriched, structures formed early and contained g

115 Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transp

116 mall GTPases and coiled-coil proteins of the golgin family help to tether COPI vesicles to Golgi memb

117 x proteins--the best characterized being the golgin family of fibrous, coiled-coil proteins and the G

118 Golgin-160 is a member of the golgin family of Golgi-localized membrane proteins.

119 Golgin-160 belongs to the golgin family of Golgi-localized proteins, which have be

120 A subset of the golgin family of large coiled-coil proteins have a GRIP

121 We show here that GCC185, a member of the Golgin family of putative tethering proteins, is a Rab9

122 Extended coiled-coil proteins of the Golgin family play prominent roles in maintaining the st

123 We have found that these VCs use different golgins for docking: C2GnT-M-carrying VC (C2GnT-M-VC) ut

124 cidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi mem

125 The golgin GM130 is thought to bind the C-terminal side of t

126 We show that the cis golgin GMAP-210 accounts for this property.

127 In this study, we show that depletion of the golgin GMAP-210 causes a loss of Golgi cisternae and acc

128 racterized genes by identifying ten putative golgins/Golgi-associated proteins amongst 8219 genes of

129 Apart from giantin and GM130, none of the golgins has yet been assigned a function in the Golgi ap

130 Golgins have been implicated in the maintenance of Golgi

131 oorly defined, and alternative functions for golgins have been proposed.

132 1p, which was accompanied by accumulation of golgin Imh1p at late Golgi, but whether and how this fun

133 plex is specifically required for recruiting golgin Imh1p to the Golgi.

134 However, the biological function of this golgin in skin is unknown.

135 iched, structures formed early and contained golgins including giantin, ranging across the entire cis

136 Some peripheral membrane Golgins, including the yeast Imh1 protein, contain the r

137 nce microscopy were employed to identify the golgins involved in the Golgi docking of vesicular compl

138 urs in the absence of GRASP65/55 when either Golgin is overexpressed, as judged by quantitative elect

139 t the pathway involving ARL1 and GRIP domain golgins is conserved in plants.

140 Lrrk interacted with the golgin Lava lamp and inhibited the interaction between L

141 The gene, designated GLP (for Golgin linked to PML), is strongly expressed as a 6-kb t

142 Golgins, long coiled-coil proteins that localize to part

143 These and other golgins may modulate the flow patterns within the Golgi

144 However, the importance of golgin-mediated tethering remains poorly defined, and al

145 proteins 55 and 65 (GRASP55 and GRASP65) and Golgin of 45 kDa and Golgi matrix protein of 130 kDa.

146 with long coiled-coil domains, like lamins, golgins, or microtubule organization center components,

147 Golgin p230 and TGN46 are separated into distinct vesicl

148 In the absence of either golgin, peri-centrosomal positioning of the Golgi appara

149 ng mitosis, we found that the formation of a golgin-positive acceptor compartment in early telophase

150 aging experiments reveal that the linking of Golgins precedes SNAREpin assembly.

151 Here we further investigate the Golgin protein Coy1 and document its function in retrogr

152 The golgin, RAB6-interacting (GORAB) protein localizes to th

153 GLP is 50% homologous to other Golgin-related proteins including the vesicle docking pr

154 f a gene with strong homology to a family of Golgin-related proteins.

155 with deletions in other putative retrograde Golgins (sgm1Delta and rud3Delta) caused strong glycosyl

156 ser predicted protein-coding genes, GOLGA8E (golgin subfamily a, 8E) and WHDC1L1 (WAS protein homolog

157 GOLGA8E belongs to the golgin subfamily of coiled-coil proteins associated with

158 Six out of 16 golgin subfamily proteins in the human genome have been

159 ifferent Rabs in addition to each end of the golgin "TATA element modulatory factor" (TMF).

160 ingly different from those bound by the p115-golgin tether in that they lacked members of the p24 fam

161 The p115-golgin tether is the best studied, and here we character

162 GORAB is a golgin that localizes predominantly at the Golgi apparat

163 GRIP domain proteins are a class of golgins that have been described in yeast and animals.

164 r to obtain clues as to the functions of the golgins, the targeting to the Golgi apparatus of two mem

165 rogression, implicating it as the only known golgin to regulate both mitosis and apoptosis.

166 s have shown, however, that mice lacking the golgin TRIP11/GMAP-210 have normal Golgi stacks, but sho

167 n this study, we report that the GRIP domain golgins, whose C termini bind the Arf-like 1 G protein o

168 We find that subsets of golgins with distinct localizations on the Golgi surface