ライフサイエンスコーパス: Gpp(NH)p

1 uanosine-5'-(beta, gamma)-imidotriphosphate (GppNHp).

2 able GTP analog 5'-guanylylimidodiphosphate (Gpp(NH)p).

3 ptides are exclusively released by only Arl3.GppNHp.

4 omplexes with a non-hydrolyzable GTP analog, GppNHp.

5 resembles other G proteins bound to GDP and GppNHp.

6 as [125]S(-)5-OH-PIPAT, was not sensitive to Gpp(NH)p.

7 ased 4-fold by MgCl2 and increased 6-fold by Gpp(NH)p.

8 [3H]glyburide in the presence of absence of Gpp(NH)p.

9 [3H]glyburide in the presence or absence of Gpp(NH)p.

10 nce or presence of guanylylimidodiphosphate (Gpp(NH)p, 10[-5] M) to determine the percentage of 5-HT1

11 The binding was reduced by GPPNHP, a nonmetabolizable GTP analogue.

12 GppNHp, a water molecule is hydrogen bonded to the side

13 pNHp non-competitively inhibited GTP gamma S/GppNHp-, AlF(4)(-)-, beta(2)-adrenoceptor plus GTP-, cho

14 Addition of Gpp(NH)p also inhibited in vitro (18)F-F13714 binding, c

15 Binding of GTPgammaS and GppNHp analogs to G(alphao) is of high affinity (K(D) 11

16 led adenylyl cyclase system to isoprenaline, Gpp(NH)p and forskolin was studied by measuring cAMP pro

17 affinity peptides are released by both Arl2.GppNHp and Arl3.GppNHp, whereas the high affinity peptid

18 PgammaS analogs (FL, 515, and TR), BODIPY FL GppNHp and BODIPY FL GTP molecules were synthesized as p

19 hown here and in other studies is related to GppNHp and is most likely nonexistent in cells.

20 s of NGF tissue to guanylylimidodiphosphate (Gpp(NH)p) and forskolin were unaffected.

21 guanosine 5'-(beta,gamma-iminotriphosphate) (GppNHp) and guanosine 5'-(3-O-thiotriphosphate) (GTPgamm

22 tant Ras isoforms (K(D) = 21 nM for KRasG12V-GppNHp) and is highly cell-permeable and metabolically s

23 adenylyl cyclase responses to isoproterenol, Gpp(NH)p, and forskolin.

24 Comparison of the structures of GppNHp- and GDP-bound RalA suggests a nucleotide-depende

25 r G alpha(s) and G alpha(i) than GTP gamma S/GppNHp as assessed by inhibition of GTP hydrolysis of re

26 nhanced the potency of MANT-GTP gamma S/MANT-GppNHp at inhibiting AC by approximately 4-8-fold.

27 The K(i) value for MANT-GppNHp at insect cell AC was 155 nm.

28 RalA bound to a non-hydrolyzable GTP analog (GppNHp) at 2.1 A resolution, providing the first structu

29 Collectively, MANT-GTP gamma S/MANT-GppNHp bind to G alpha(s)- and G alpha(i)-proteins with

30 thiol (GTPgammaS) or the 3' ribose-hydroxyl (GppNHp) bond to relieve the quenching of BODIPY fluoresc

31 tsY association previously observed with the GppNHp-bound form.

32 range and remarkably different for GDP- and GppNHp-bound forms of the G domain, indicating that the

33 Our results show that GppNHp-bound proteins exhibit significant state 1 popula

34 Interestingly, we find that GppNHp-bound RhoC only shows differences in its switch I

35 Increasing concentrations of Gpp(NH)p, but not ATP, decreased the specific binding of

36 inhibitors had reduced affinity to KRAS Q61R-GppNHp, but not to WT and other mutants.

37 MANT-GTP gamma S and MANT-GppNHp competitively inhibited forskolin/Mn(2+)-stimulat

38 0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP an

39 Instead, MANT-GTP gamma S/MANT-GppNHp constitute a novel class of potent competitive AC

40 E) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence of a moderate amount of

41 A loss of affinity was observed for KRAS-GppNHp, due in part to rearrangements in switch-II, wher

42 r %R(H) (51% vs 44%) for beta(2)AR-G(s), but GppNHp failed to convert most of these to the low-affini

43 The affinities of BODIPY FL GppNHp for all four G(alpha) subunits are 10-fold lower

44 TP or a commonly used nonhydrolyzable analog GppNHp (guanosine-5'-[(beta,gamma)-imido] triphosphate).

45 MANT-GTP gamma S/MANT-GppNHp had lower affinities for G alpha(s) and G alpha(i

46 guanosine-5'-(betagamma-imino)triphosphate (GppNHp) has been determined at a resolution of 1.5 A.

47 Mn(2+) blocked AC inhibition by GTP gamma S/GppNHp in S49 cyc(-) membranes but enhanced the potency

48 Ran x GppNHp in the complex shows extensive structural rearran

49 , fusion is still sensitive to GTPgammaS and GppNHp, indicating that there is a second specific GTPas

50 h peptidomimetics were able to attenuate the Gpp(NH)p-induced shift to the low-affinity state to a gr

51 uanosine 5'-(beta, gamma-imido)triphosphate (Gpp(NH)p), inhibits binding of 125I-glucagon to the wild

52 karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP analogue.

53 cess in human H-Ras complexed with GTP mimic GppNHp is global, encompassing most of the GTPase cataly

54 MANT-GTP gamma S/MANT-GppNHp non-competitively inhibited GTP gamma S/GppNHp-,

55 ivating protein for Ypt7p:GTP), GTPgammaS or GppNHp (non-hydrolyzable nucleotides), and mutant forms

56 lizes the conformation observed in the H-Ras-GppNHp/NOR1A complex, and PEG, DTE, and DTT stabilize th

57 sine 5'-[beta,gamma-imido]triphosphate (MANT-GppNHp) on G alpha(s)- and G alpha(i)-protein-mediated s

58 Even with Gpp(NH)p present, 3 and 4 were able to return the RH/RL

59 The crystal structure of KRAS Q61R-GppNHp reported here revealed that access to the switch-

60 tive affinities or amounts of high-affinity, Gpp(NH)p-sensitive binding of agonist.

61 guanosine 5'-(beta,gamma-imido)triphosphate (GppNHp) shifted the agonist-bound receptor into a G prot

62 -0.3 in remote myocardium; P<0.05), although GppNHp-stimulated cAMP production was equivocally reduce

63 In Sec4-GppNHp, structural features common to active Rab protein

64 Gpp(NH)p together with sodium ion markedly decreased bin

65 res of atlastin-1 bound to GDP.AlF(4)(-) and GppNHp, uncovering an intramolecular arginine finger tha

66 These effects were observed when either Gpp(NH)p was absent or present and they were comparable

67 es observed in the respective controls where Gpp(NH)p was absent.

68 These effects were observed when Gpp(NH)p was either absent or present, and they were ana

69 AC inhibition by MANT-GTP gamma S/MANT-GppNHp was not due to G alpha(s) inhibition because it w

70 es are released by both Arl2.GppNHp and Arl3.GppNHp, whereas the high affinity peptides are exclusive

71 [3H]glyburide (in the presence or absence of Gpp(NH)p which blocks binding to low-affinity sites) to