ライフサイエンスコーパス: Gpp(NH)p

1 Gpp(NH)p together with sodium ion markedly decreased bin

2 GppNHp, a water molecule is hydrogen bonded to the side

3 tive affinities or amounts of high-affinity, Gpp(NH)p-sensitive binding of agonist.

4 -0.3 in remote myocardium; P<0.05), although GppNHp-stimulated cAMP production was equivocally reduce

5 RalA bound to a non-hydrolyzable GTP analog (GppNHp) at 2.1 A resolution, providing the first structu

6 omplexes with a non-hydrolyzable GTP analog, GppNHp.

7 res of atlastin-1 bound to GDP.AlF(4)(-) and GppNHp, uncovering an intramolecular arginine finger tha

8 resembles other G proteins bound to GDP and GppNHp.

9 range and remarkably different for GDP- and GppNHp-bound forms of the G domain, indicating that the

10 Binding of GTPgammaS and GppNHp analogs to G(alphao) is of high affinity (K(D) 11

11 , fusion is still sensitive to GTPgammaS and GppNHp, indicating that there is a second specific GTPas

12 affinity peptides are released by both Arl2.GppNHp and Arl3.GppNHp, whereas the high affinity peptid

13 es are released by both Arl2.GppNHp and Arl3.GppNHp, whereas the high affinity peptides are exclusive

14 ptides are exclusively released by only Arl3.GppNHp.

15 r %R(H) (51% vs 44%) for beta(2)AR-G(s), but GppNHp failed to convert most of these to the low-affini

16 ased 4-fold by MgCl2 and increased 6-fold by Gpp(NH)p.

17 These effects were observed when either Gpp(NH)p was absent or present and they were comparable

18 PgammaS analogs (FL, 515, and TR), BODIPY FL GppNHp and BODIPY FL GTP molecules were synthesized as p

19 The affinities of BODIPY FL GppNHp for all four G(alpha) subunits are 10-fold lower

20 able GTP analog 5'-guanylylimidodiphosphate (Gpp(NH)p).

21 nce or presence of guanylylimidodiphosphate (Gpp(NH)p, 10[-5] M) to determine the percentage of 5-HT1

22 s of NGF tissue to guanylylimidodiphosphate (Gpp(NH)p) and forskolin were unaffected.

23 thiol (GTPgammaS) or the 3' ribose-hydroxyl (GppNHp) bond to relieve the quenching of BODIPY fluoresc

24 uanosine-5'-(beta, gamma)-imidotriphosphate (GppNHp).

25 guanosine 5'-(beta,gamma-iminotriphosphate) (GppNHp) and guanosine 5'-(3-O-thiotriphosphate) (GTPgamm

26 led adenylyl cyclase system to isoprenaline, Gpp(NH)p and forskolin was studied by measuring cAMP pro

27 adenylyl cyclase responses to isoproterenol, Gpp(NH)p, and forskolin.

28 MANT-GTP gamma S and MANT-GppNHp competitively inhibited forskolin/Mn(2+)-stimulat

29 The K(i) value for MANT-GppNHp at insect cell AC was 155 nm.

30 nhanced the potency of MANT-GTP gamma S/MANT-GppNHp at inhibiting AC by approximately 4-8-fold.

31 Collectively, MANT-GTP gamma S/MANT-GppNHp bind to G alpha(s)- and G alpha(i)-proteins with

32 Instead, MANT-GTP gamma S/MANT-GppNHp constitute a novel class of potent competitive AC

33 MANT-GTP gamma S/MANT-GppNHp had lower affinities for G alpha(s) and G alpha(i

34 MANT-GTP gamma S/MANT-GppNHp non-competitively inhibited GTP gamma S/GppNHp-,

35 AC inhibition by MANT-GTP gamma S/MANT-GppNHp was not due to G alpha(s) inhibition because it w

36 sine 5'-[beta,gamma-imido]triphosphate (MANT-GppNHp) on G alpha(s)- and G alpha(i)-protein-mediated s

37 cess in human H-Ras complexed with GTP mimic GppNHp is global, encompassing most of the GTPase cataly

38 [3H]glyburide (in the presence or absence of Gpp(NH)p which blocks binding to low-affinity sites) to

39 [3H]glyburide in the presence of absence of Gpp(NH)p.

40 [3H]glyburide in the presence or absence of Gpp(NH)p.

41 Addition of Gpp(NH)p also inhibited in vitro (18)F-F13714 binding, c

42 Increasing concentrations of Gpp(NH)p, but not ATP, decreased the specific binding of

43 Comparison of the structures of GppNHp- and GDP-bound RalA suggests a nucleotide-depende

44 ivating protein for Ypt7p:GTP), GTPgammaS or GppNHp (non-hydrolyzable nucleotides), and mutant forms

45 E) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence of a moderate amount of

46 lizes the conformation observed in the H-Ras-GppNHp/NOR1A complex, and PEG, DTE, and DTT stabilize th

47 r G alpha(s) and G alpha(i) than GTP gamma S/GppNHp as assessed by inhibition of GTP hydrolysis of re

48 Mn(2+) blocked AC inhibition by GTP gamma S/GppNHp in S49 cyc(-) membranes but enhanced the potency

49 pNHp non-competitively inhibited GTP gamma S/GppNHp-, AlF(4)(-)-, beta(2)-adrenoceptor plus GTP-, cho

50 In Sec4-GppNHp, structural features common to active Rab protein

51 Interestingly, we find that GppNHp-bound RhoC only shows differences in its switch I

52 h peptidomimetics were able to attenuate the Gpp(NH)p-induced shift to the low-affinity state to a gr

53 tsY association previously observed with the GppNHp-bound form.

54 as [125]S(-)5-OH-PIPAT, was not sensitive to Gpp(NH)p.

55 uanosine 5'-(beta, gamma-imido)triphosphate (Gpp(NH)p), inhibits binding of 125I-glucagon to the wild

56 guanosine 5'-(beta,gamma-imido)triphosphate (GppNHp) shifted the agonist-bound receptor into a G prot

57 guanosine-5'-(betagamma-imino)triphosphate (GppNHp) has been determined at a resolution of 1.5 A.

58 These effects were observed when Gpp(NH)p was either absent or present, and they were ana

59 es observed in the respective controls where Gpp(NH)p was absent.

60 karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP analogue.

61 Even with Gpp(NH)p present, 3 and 4 were able to return the RH/RL

62 0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP an

63 Ran x GppNHp in the complex shows extensive structural rearran