ライフサイエンスコーパス: H. capsulatum

1 H. capsulatum conidia were also cytotoxic to amoebae.

2 H. capsulatum expresses several iron acquisition mechani

3 H. capsulatum is known to secrete Fe(III)-binding hydrox

4 H. capsulatum must compete with the host to acquire the

5 H. capsulatum remains an important pathogen among immuno

6 H. capsulatum uses different host receptors for binding

7 H. capsulatum var. farciminosum was found within the Sou

8 H. capsulatum yeasts expressed CBP1 in all of these sett

9 H. capsulatum-infected CCR2(-/-) mice manifested defects

10 capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolat

11 In addition, after H. capsulatum yeasts were subjected to a calcium-free sh

12 ibited additive fungistatic activity against H. capsulatum yeasts.

13 tein (BPI) also mediated fungistasis against H. capsulatum in a concentration-dependent manner.

14 exhibited no significant fungistasis against H. capsulatum yeasts.

15 ation of WGA-Fc fully protected mice against H. capsulatum, correlating with a reduction in lung, spl

16 nce, the capacity of IgG MAbs to agglutinate H. capsulatum significantly impacted pathogenic mechanis

17 atum var. capsulatum, (iii) Central American H. capsulatum var. capsulatum, (iv) South American H. ca

18 ified six clades: (i) class 1 North American H. capsulatum var. capsulatum, (ii) class 2 North Americ

19 var. capsulatum, (ii) class 2 North American H. capsulatum var. capsulatum, (iii) Central American H.

20 With the exception of the South American H. capsulatum var. capsulatum group A clade, genetic dis

21 iminosum was found within the South American H. capsulatum var. capsulatum group A clade.

22 sulatum var. capsulatum, (iv) South American H. capsulatum var. capsulatum group A, (v) South America

23 var. capsulatum group A, (v) South American H. capsulatum var. capsulatum group B, and (vi) H. capsu

24 These results suggest that GH17 encodes an H. capsulatum antigen that may be useful for the diagnos

25 generated monoclonal antibodies (MAbs) to an H. capsulatum surface-expressed heat shock protein of 60

26 rter fusions analysed in B. dermatitidis and H. capsulatum confirmed that BAD1 is transcriptionally r

27 hod for the detection of B. dermatitidis and H. capsulatum from culture isolates and directly from cl

28 detect and differentiate B. dermatitidis and H. capsulatum from culture isolates and directly from cl

29 e dehydrogenase genes of B. dermatitidis and H. capsulatum, respectively.

30 oydii as well as against B. dermatitidis and H. capsulatum.

31 athogenicity in Blastomyces dermatitidis and H. capsulatum.

32 at importance to its success or failure, and H. capsulatum is good at finding or making the right env

33 Treatment of naive and H. capsulatum-immune mice with caspase inhibitors decrea

34 ins, cathepsin G, and BPI are the major anti-H. capsulatum effector molecules in the azurophil granul

35 Because H. capsulatum displays a considerable array of virulence

36 We report a case of sinusitis caused by H. capsulatum in a patient with AIDS.

37 l three families of siderophores excreted by H. capsulatum.

38 is morphologic switch, which is exhibited by H. capsulatum and a group of evolutionarily related fung

39 esults are consistent with the expression by H. capsulatum of both enzymatic ferric reductase and non

40 C can override one of the strategies used by H. capsulatum yeasts to survive intracellularly within M

41 that immunoglobulin Gs (IgGs) to Hsp60 cause H. capsulatum aggregation dependent on the (i) concentra

42 Consequently, H. capsulatum was selectively deprived of Zn, thereby ha

43 eased the capacity of DC to kill and degrade H. capsulatum yeasts.

44 -induced resistance against B. dermatitidis, H. capsulatum, and C. posadasii.

45 mples but the Isolator system alone detected H. capsulatum in seven samples.

46 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients

47 istoplasma capsulatum; both systems detected H. capsulatum in seven samples but the Isolator system a

48 on of Tregs in the lungs prior to and during H. capsulatum infection.

49 We propose that during H. capsulatum infection of mammalian hosts, spontaneous

50 hat of cells from mice immunized with either H. capsulatum recombinant Hsp70 or bovine serum albumin.

51 g the 29 horses with suspected cases of EZL, H. capsulatum var. farciminosum was confirmed by extract

52 tly survive within macrophages, facilitating H. capsulatum translocation from the lung into the lymph

53 Finally, H. capsulatum displays morphotype-specific expression of

54 86% for B. dermatitidis and 100% and 73% for H. capsulatum compared with the results for culture.

55 midine biosynthetic pathway is essential for H. capsulatum growth and virulence.

56 are the predominant infectious particle for H. capsulatum and are the first cell type encountered by

57 Eighteen of 19 blood cultures positive for H. capsulatum grew in both IS and MFL, although the time

58 Specific probes for H. capsulatum, B. dermatitidis, C. immitis, P. brasilien

59 ciens-mediated mutagenesis, and screened for H. capsulatum insertional mutants that were unable to su

60 and 100% specificity and 94% sensitivity for H. capsulatum.

61 he Accuprobe DNA and the exoantigen test for H. capsulatum var. capsulatum.

62 on with AmB may be useful as a treatment for H. capsulatum in immunodeficient hosts.

63 ssaying for beta-galactosidase activity from H. capsulatum transformants, we identified a 102-bp regi

64 The dimorphic probe hybridized with DNA from H. capsulatum, B. dermatitidis, C. immitis, P. brasilien

65 from inorganic or organic ferric salts, from H. capsulatum hydroxamate siderophores, or from host Fe(

66 A "dimorphic" fungus, H. capsulatum exists as a saprophytic mold in soil and c

67 ell as concordance of four gene genealogies, H. capsulatum could be considered to harbor six species

68 To understand how H. capsulatum responds to RNS, we determined the transcr

69 However, H. capsulatum can establish persistent infections, indic

70 In a screen to identify H. capsulatum genes required for lysis of bone marrow-de

71 In H. capsulatum BAD1 transformants, yeast phase-specific e

72 gical role of this immunodominant antigen in H. capsulatum, enzymatic assays were performed to determ

73 m, we designed a strategy to disrupt CBP1 in H. capsulatum using a telomeric linear plasmid and a two

74 g the balance between Treg and Th17 cells in H. capsulatum infection.

75 Here we show that a 250-fold difference in H. capsulatum susceptibility between inbred mouse strain

76 tion and is also expressed differentially in H. capsulatum strains of different virulence levels.

77 Apoptosis was diminished in H. capsulatum-infected gld/gld and TNF-alpha-deficient m

78 g DNA, which has not been previously done in H. capsulatum and has been challenging in some other pat

79 indicate the importance of Hcl1 function in H. capsulatum replication in the harsh growth environmen

80 be removed without compromising function in H. capsulatum, allowing construction of a substantially

81 pression of functional beta-galactosidase in H. capsulatum.

82 ntial for functional expression of a gene in H. capsulatum.

83 ve DPPIV homologs (HcDPPIVA and HcDPPIVB) in H. capsulatum based on a homology search with Aspergillu

84 rst nuclear DNA binding factor identified in H. capsulatum which binds to target sequences in a phase

85 t sulfur metabolism influences morphology in H. capsulatum.

86 rature-responsive transcriptional network in H. capsulatum, which switches from a filamentous form in

87 on, as well as in a substantial reduction in H. capsulatum burden at 35 and 90 days postinfection.

88 perimentally directed allelic replacement in H. capsulatum, or in any dimorphic systemic fungal patho

89 ommon ancestor, Wor1 in C. albicans, Ryp1 in H. capsulatum, and Mit1 in S. cerevisiae are transcripti

90 temperature-regulated morphologic switch in H. capsulatum.

91 egulates the yeast to mycelial transition in H. capsulatum.

92 quisition in pathogenic organisms, including H. capsulatum, is a highly regulated process.

93 colony-stimulating factor (GM-CSF), inhibit H. capsulatum growth in macrophages.

94 Interestingly, H. capsulatum growth was restricted in mice lacking the

95 on by transformation of an HAG1 plasmid into H. capsulatum.

96 In response to excess iron, H. capsulatum represses transcription of genes involved

97 tection in patients' sera of a 69- to 70-kDa H. capsulatum var. capsulatum-specific antigen which app

98 nce, IgG1 and IgG2a MAbs to Hsp60 can modify H. capsulatum pathogenesis in part by altering the intra

99 terations in the metal homeostasis of murine H. capsulatum-infected macrophages that were exposed to

100 Conversely, mycelial H. capsulatum did not produce CBP, a finding that correl

101 lture media inhibited the growth of mycelial H. capsulatum but had no effect on yeast growth in vitro

102 infection with H. capsulatum conidia but not H. capsulatum yeast cells.

103 Although the ability of H. capsulatum to prevent acidification of the macrophage

104 dentical disease occurring in the absence of H. capsulatum seropositivity.

105 After a lethal challenge of H. capsulatum, CD40L-/- mice were not substantially diff

106 , but small budding yeasts characteristic of H. capsulatum were present.

107 L-4 was associated with delayed clearance of H. capsulatum yeast and increased fungal burden.

108 be used to expedite culture confirmation of H. capsulatum in regions in which PDH is endemic.

109 ival and a reduction in the colony counts of H. capsulatum in internal organs at 14 days after infect

110 f naive and immune mice during the course of H. capsulatum infection.

111 Remarkably, despite a decrease of H. capsulatum CFU in lungs of CD4+ plus CD8+-deficient m

112 bacteria; however, the time to detection of H. capsulatum is increased.

113 the first reports of the direct detection of H. capsulatum var. farciminosum in equine blood and at h

114 The mean times to the detection of H. capsulatum were 8 days (range, 4 to 13 days) for MYCO

115 d to a single location in the genomic DNA of H. capsulatum.

116 were first infected with a sublethal dose of H. capsulatum (1 x 10(5)) and then reinfected 3 wk later

117 reinfected 3 wk later with a lethal dose of H. capsulatum (6 x 10(5)).

118 0L-/- mice infected with a sublethal dose of H. capsulatum survived infection, whereas all mice infec

119 initially infected with a sublethal dose of H. capsulatum were protected from secondary infection wi

120 om secondary infection with a lethal dose of H. capsulatum, demonstrating that CD40L is not required

121 To identify phase-regulated genes of H. capsulatum, we carried out expression analyses by usi

122 Chloroquine inhibits growth of H. capsulatum by pH-dependent iron deprivation, whereas

123 Growth of H. capsulatum mycelia in chemically defined minimal medi

124 Growth of H. capsulatum yeast in chemically defined minimal medium

125 -1, HNP-2, and HNP-3 inhibited the growth of H. capsulatum yeasts in a concentration-dependent manner

126 rphis by IFN-gamma and effective handling of H. capsulatum.

127 to a 60-kDa native antigen in immunoblots of H. capsulatum yeast antigen extract.

128 vival in response to a sublethal inoculum of H. capsulatum The absence of myeloid HIF-1alpha did not

129 were designed to amplify the Hcp100 locus of H. capsulatum.

130 neration of excess interleukin-4 in lungs of H. capsulatum-infected CCR2(-/-) mice is at least partia

131 lecular genetic analysis and manipulation of H. capsulatum, which have proven useful for examination

132 nificantly impacted pathogenic mechanisms of H. capsulatum during macrophage infection, and the effec

133 We have isolated a UV-induced mutant of H. capsulatum, resulting in nonreverting uracil auxotrop

134 of V-ATPase function in the pathogenicity of H. capsulatum, in iron homeostasis and in fungal dimorph

135 nii selected for, or induced, a phenotype of H. capsulatum that caused a persistent murine lung infec

136 The presence of H. capsulatum var. farciminosum DNA was confirmed by seq

137 ngs of infected mice, aberrant processing of H. capsulatum within macrophages, and immobilization of

138 The production of H. capsulatum-induced interferon-gamma and TNF-alpha was

139 we determined the transcriptional profile of H. capsulatum to *NO-generating compounds using a shotgu

140 Intracellular proliferation of H. capsulatum was measured in alveolar macrophages and p

141 important for survival and proliferation of H. capsulatum within macrophages.

142 ue, was sufficient to increase resistance of H. capsulatum to RNS in culture.

143 formation on the hydroxamate siderophores of H. capsulatum.

144 tants were generated in a virulent strain of H. capsulatum by optimization of Agrobacterium tumefacie

145 virulence, thermal-sensitive Downs strain of H. capsulatum lacked detectable p30 binding activity in

146 xposure of an avirulent laboratory strain of H. capsulatum to A. castellanii selected for, or induced

147 and M phases of the virulent G217B strain of H. capsulatum were visualized by their capability for in

148 e activity to UV-mutagenized ura5 strains of H. capsulatum.

149 idence for the existence of two subgroups of H. capsulatum var. farciminosum.

150 N response is required for full virulence of H. capsulatum in mice.

151 s both surface localized in the cell wall of H. capsulatum and released into the culture medium.

152 The adverse effects of IL-4 on H. capsulatum elimination were not observed during the e

153 nation effects of the antibodies to Hsp60 on H. capsulatum yeast cells by light microscopy, flow cyto

154 uracil auxotrophy due to a ura5 mutation on H. capsulatum virulence in both cell culture and whole-a

155 that human dendritic cells (DC) phagocytose H. capsulatum yeasts and, unlike human macrophages (Mo)

156 considered a resident of the phagolysosome, H. capsulatum may also reside in a modified phagosome wi

157 s, the site preference measured for purified H. capsulatum P450nor was not constant, increasing from

158 Here, we report H. capsulatum ferric reduction activities in whole yeast

159 robial effector nitric oxide (*NO) restricts H. capsulatum replication.

160 l, this hypothesis has not been tested since H. capsulatum mutants that experience decreased phagosom

161 In vivo, Zn supplementation and subsequent H. capsulatum infection supressed MHCII on DCs, enhanced

162 These findings indicate that H. capsulatum conidia and yeast can produce melanin or m

163 We propose that H. capsulatum uses the pathways identified here to cope

164 The H. capsulatum siderophore dimerum acid and the structura

165 s in H protein expression levels between the H. capsulatum classes, with a correlation between secret

166 ntified an insertion mutation disrupting the H. capsulatum homolog of 3-hydroxy-methylglutaryl coenzy

167 robe used against C. immitis DNA and for the H. capsulatum probe used against Candida albicans DNA.

168 nt with observations in other organisms, the H. capsulatum hcl1 mutant was unable to grow on leucine

169 ) were too distant to unequivocally root the H. capsulatum tree.

170 light, with the notable distinction that the H. capsulatum circuit responds to temperature.

171 reted beta-glucosidase activities from three H. capsulatum restriction fragment length polymorphism (

172 Thus, H. capsulatum provokes an orderly modulation of the infl

173 inding MAb and were similar in appearance to H. capsulatum yeast cells.

174 DNA, they did not necessarily correspond to H. capsulatum open reading frames.

175 tive immunogen against pulmonary exposure to H. capsulatum.

176 genous IL-4 modulates protective immunity to H. capsulatum by delaying clearance of the organism but

177 interleukin (IL)-17 and IL-23 on immunity to H. capsulatum infection in mice.

178 CD40L was essential for primary immunity to H. capsulatum infection.

179 a critical element of protective immunity to H. capsulatum.

180 IgG) isotype monoclonal antibodies (MAbs) to H. capsulatum Hsp60.

181 mice that received methamphetamine prior to H. capsulatum infection were immunologically impaired, w

182 arphis drove IL-10 production in response to H. capsulatum IL-10 inhibited Mvarphi control of fungal

183 ic/F bottle did not recover M. tuberculosis, H. capsulatum, or C. neoformans isolates.

184 Uracil auxotrophs of two H. capsulatum restriction fragment length polymorphism c

185 Wild-type H. capsulatum grows as filaments at room temperature and

186 capsulatum var. capsulatum group B, and (vi) H. capsulatum var. duboisii.

187 c assays were performed to determine whether H. capsulatum contained a beta-glucosidase enzyme activi

188 ave revealed an important mechanism by which H. capsulatum thwarts the host immune system.

189 against sublethal and lethal challenges with H. capsulatum yeasts.

190 ntained abundant yeast forms consistent with H. capsulatum.

191 asma but not morphologically consistent with H. capsulatum.

192 Substrate gels with H. capsulatum culture supernatants revealed beta-glucosi

193 FN-gamma(-/-) mice previously immunized with H. capsulatum and treated with amphotericin B at the tim

194 Splenocytes from mice immunized with H. capsulatum yeasts responded to purified CBP in prolif

195 activity were identified by incubation with H. capsulatum yeasts for 24 h and by quantifying the sub

196 40L-/-) and CD40L+/+ mice were infected with H. capsulatum and assessed for various parameters.

197 Tissues from mice infected with H. capsulatum and from biopsy specimens from a patient w

198 SCID mice infected with H. capsulatum and treated with IL-12 showed an increase

199 cytes and alveolar macrophages infected with H. capsulatum was inhibited by the addition of physiolog

200 prolonged the survival of mice infected with H. capsulatum.

201 induced in macrophages during infection with H. capsulatum conidia but not H. capsulatum yeast cells.

202 i) the immune response during infection with H. capsulatum is controlled via mechanisms independent o

203 fested a high mortality after infection with H. capsulatum, although TNFR1-/- mice were more suscepti

204 mediating protection against infection with H. capsulatum, CD40L-deficient (CD40L-/-) and CD40L+/+ m

205 ributes to host resistance to infection with H. capsulatum.

206 the lungs of mice infected intranasally with H. capsulatum.

207 that normal mice infected intravenously with H. capsulatum and treated with interleukin-12 (IL-12) at

208 s restored in the presence of wild-type (WT) H. capsulatum yeasts, or the hydroxamate siderophore, rh