ライフサイエンスコーパス: HDX-MS

1 HDX MS revealed conformational changes at the resolution

2 HDX-MS also shows a dramatic increase in the protein-lip

3 HDX-MS analysis shows that SufE binds near the SufS acti

4 HDX-MS and MD of holo-PhuS indicate an overall reduction

5 HDX-MS identifies a potential Galpha interaction site in

6 HDX-MS revealed that the apo-PhuS C-terminal alpha6/alph

7 HDX-MS reveals that while the dominant structural change

8 HDX-MS thus holds exceptional promise as an enabling ana

9 a given peptide's isotope distribution, and HDX MS data analysis methods were developed accordingly.

10 sults also highlight the utility of applying HDX-MS to ADCs to gain a molecular level insight into th

11 ycan level opens the possibility of applying HDX-MS to monitor glycan interactions and dynamics.

12 Here, we describe an automated HDX-MS platform that operates with a parallel, two-trap,

13 Although the proteolysis-based HDX-MS approach is most commonly used, the "top-down" ap

14 This technique will benefit HDX MS measurements for any protein that contains one or

15 rmational heterogeneity was also assessed by HDX MS isotopic distribution.

16 r the FcRn binding interface as indicated by HDX MS and structural modeling; however, HC Met257 oxida

17 nge of proteins amenable to interrogation by HDX MS continues to expand at an accelerating pace, ther

18 the largest protein assembly yet accessed by HDX-MS, with sequence resolution of segments of as few a

19 ity upon antibody binding were identified by HDX-MS, and five different peptides over the same three

20 y map densities with constraints provided by HDX-MS and crosslinking, allowing us to obtain valuable

21 In a conventional HDX-MS workflow, disulfide bonds are reduced chemically

22 X-ray crystallography, HDX-MS and SPR analysis confirmed that the CDR regions o

23 Differential HDX-MS of isotopically labeled RT in the presence of the

24 -retest repeatability study for differential HDX-MS experiments implemented at each of two laboratori

25 Successful applications of differential HDX-MS include the characterization of protein-ligand bi

26 A single differential HDX-MS data set (protein +/- ligand) is often comprised

27 kbone protection pattern generated by direct HDX-MS/MS is in excellent agreement with the known cryst

28 ation of the new methodology to the top-down HDX MS characterization of a small (99 residue long) dis

29 protection pattern deduced from the top-down HDX MS measurements carried out under native conditions

30 roteins amenable to the analysis by top-down HDX MS still remains limited, with the protein size and

31 Resolving isotopic fine structure during HDX MS therefore permits direct monitoring of HDX, which

32 electrochemical reduction efficiency during HDX-MS analysis of two particularly challenging disulfid

33 successful electrochemical reduction during HDX-MS analysis of both a small exceptional tightly disu

34 icles can be easily incorporated in existing HDX MS workflows to provide more peptide coverage in exp

35 d glycoproteins however pose a challenge for HDX-MS.

36 clude that An-PEP is an archetype enzyme for HDX-MS, highly complementary to pepsin, and especially p

37 eptidase from Aspergillus niger (An-PEP) for HDX-MS.

38 ndom and gross errors, automated systems for HDX-MS analysis have become routine in many laboratories

39 show that under conditions commonly used for HDX-MS, acetamido groups within glycan chains retain a s

40 ted, however, the data analysis workflow for HDX-MS data with resolved isotopic fine structure is dis

41 Furthermore, HDX-MS revealed that a MinE mutant (D45A/V49A), previous

42 In this article, an improved HDX MS platform with fully automated data processing is

43 lenges in using electrochemical reduction in HDX-MS analyses and provide possible conditions to atten

44 erium levels at single residue resolution in HDX-MS experiments.

45 emical reduction as a substitute for TCEP in HDX-MS analyses.

46 s often comprised of more than 40 individual HDX-MS experiments.

47 gh the application of automated (and manual) HDX-MS has become common, there are only a handful of st

48 Raman (RR), hydrogen-deuterium exchange MS (HDX-MS) methods, and molecular dynamics (MD).

49 pplied hydrogen-deuterium (H/D) exchange MS (HDX-MS) of full-length Escherichia coli DXPS to provide

50 hydrogen-deuterium exchange coupled with MS (HDX-MS), we probed the RcnR structure in the presence of

51 Here, we present a novel HDX-MS workflow for analysis of the conformational dynam

52 Da glycoprotein transferrin in the course of HDX MS experiments is carried out using electron capture

53 ploited the aforementioned unique feature of HDX MS in combination with the ability of MS to isolate

54 h states previously remained out of reach of HDX MS.

55 lished describing a cross-site comparison of HDX-MS experiments.

56 Comparison of HDX-MS results for apo GroEL and GroEL-ATPgammaS enables

57 s C to 55 degrees C for the interrogation of HDX-MS.

58 The successful outcome of HDX-MS analyses depends on the sample preparation condit

59 systematic evaluation of the performance of HDX-MS experiments, and no reports have been published d

60 These results highlight the utility of HDX-MS for interrogating the higher-order structure of A

61 ing electrochemical reduction into an online HDX-MS workflow.

62 ESI interface, allowing for online gas-phase HDX-MS analysis of peptides and proteins separated on a

63 rinopeptide B, confirmed that this gas-phase HDX-MS approach allows for labeling of sites (heteroatom

64 Furthermore, online gas-phase HDX-MS could be performed in tandem with ion mobility se

65 Results from gas-phase HDX-MS of peptides using the aqueous ND3/D2O as HDX reag

66 pectrometer immediately after ESI (gas-phase HDX-MS) and show utility for studying the primary and hi

67 us) not accessed by classical solution-phase HDX-MS.

68 Previous HDX MS methods did not resolve these isotopomers, requir

69 ly unattended analysis of the entire protein HDX MS data set starting from ion detection and peptide

70 ion dissociation is verified using redundant HDX-MS data generated by FTICR-MS.

71 rogen/deuterium exchange mass spectrometric (HDX-MS) investigation of an antibody-drug conjugate (ADC

72 drogen-deuterium exchange mass spectrometry (HDX MS) experiments.

73 drogen/deuterium exchange mass spectrometry (HDX MS) was used in two case studies to evaluate the imp

74 drogen-deuterium exchange mass spectrometry (HDX MS) was used to interrogate the conformational impac

75 drogen/deuterium exchange mass spectrometry (HDX MS), native mass spectrometry, and negative-staining

76 ium exchange coupled with mass spectrometry (HDX MS).

77 drogen-deuterium exchange mass spectrometry (HDX-MS) and chemical crosslinking.

78 drogen/deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics studies revealed that Ca(

79 drogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis using full-length

80 drogen-deuterium exchange mass spectrometry (HDX-MS) and SPR interaction analysis on a library of lys

81 drogen/deuterium exchange mass spectrometry (HDX-MS) approaches have been used to inform on the globa

82 gen deuterium exchange by mass spectrometry (HDX-MS) as a function of temperature in a thermophilic d

83 drogen/deuterium exchange mass spectrometry (HDX-MS) by comparing the deuterium uptake in the bound a

84 erium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihyd

85 drogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a powerful analytical technique w

86 erium exchange coupled to mass spectrometry (HDX-MS) has emerged as a technique for studying glycopro

87 drogen/deuterium exchange mass spectrometry (HDX-MS) in the protein therapeutic field is undisputed;

88 drogen-deuterium exchange mass spectrometry (HDX-MS) indicates that the domain contains limited hydro

89 ium exchange coupled with mass spectrometry (HDX-MS) is an information-rich biophysical method for th

90 drogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical i

91 drogen/deuterium exchange mass spectrometry (HDX-MS) is now a routinely used technique to inform on p

92 drogen/deuterium-exchange mass spectrometry (HDX-MS) provides detailed insight into the structural dy

93 drogen/deuterium exchange mass spectrometry (HDX-MS) requires effective and rapid reduction of disulf

94 ium exchange monitored by mass spectrometry (HDX-MS) requires reduction of the disulfide bonds under

95 ange studies coupled with mass spectrometry (HDX-MS) show that upon interaction with lipid, the centr

96 drogen/deuterium exchange mass spectrometry (HDX-MS) to access E. coli chaperonin GroEL conformation.

97 drogen/deuterium-exchange mass spectrometry (HDX-MS) to analyze conformational changes in NhaA upon L

98 drogen/deuterium exchange mass spectrometry (HDX-MS) to characterize interactions of low affinity pep

99 drogen/deuterium exchange mass spectrometry (HDX-MS) to characterize SufS-SufE interactions and prote

100 drogen deuterium exchange mass spectrometry (HDX-MS) to identify critical residues for RNA interactio

101 drogen-deuterium exchange mass spectrometry (HDX-MS) to investigate membrane protein conformational d

102 erium exchange coupled to mass spectrometry (HDX-MS) to investigate the effects on HBV capsids of bin

103 drogen-deuterium exchange mass spectrometry (HDX-MS) to map the impact of the new disulfide bond on t

104 drogen-deuterium exchange mass spectrometry (HDX-MS) to reveal conformational changes accompanying me

105 drogen-deuterium exchange-mass spectrometry (HDX-MS) was employed to analyze the backbone dynamics pr

106 erium exchange coupled to mass spectrometry (HDX-MS) we rule out a model in which the two forms are i

107 drogen-Deuterium Exchange-Mass Spectrometry (HDX-MS) we show that Ric-8A disrupts the secondary struc

108 ium exchange coupled with mass spectrometry (HDX-MS), and facilitating interpretation of the data wit

109 drogen/deuterium exchange-mass spectrometry (HDX-MS), especially when applied to larger proteins (ove

110 drogen/deuterium exchange mass spectrometry (HDX-MS), fast photochemical oxidation of proteins (FPOP)

111 drogen/deuterium exchange mass spectrometry (HDX-MS).

112 drogen/deuterium exchange mass spectrometry (HDX-MS).

113 drogen/deuterium exchange mass spectrometry (HDX-MS).

114 d in the adoption of isotopic fine structure HDX-MS by providing an intuitive workflow and interface

115 Isotopic fine structure HDX-MS offers the potential to increase sequence coverag

116 In the second case study, HDX MS demonstrated that oxidation of the two complement

117 Until this study, HDX MS experiments employed mass spectral resolving powe

118 Together, these results suggested that HDX MS is a powerful tool for evaluating the impact of i

119 Our results demonstrate that HDX-MS can validate and map weak peptide-protein interac

120 We show that HDX-MS can identify regions in the two epigenetic regula

121 Together, these results suggest that HDX-MS of His C2-hydrogens is a promising new method for

122 The HDX MS profile of receptor bound to carazolol is consist

123 The utility of the HDX MS platform is demonstrated by exploring the conform

124 The HDX-MS workflow was optimized to accurately detect low-a

125 mplementing an electrochemical cell into the HDX-MS workflow.

126 for increasing the spatial resolution of the HDX-MS experiment, capable of yielding high fragmentatio

127 for the first time the applicability of the HDX-MS technique to monitor structural changes due to me

128 handling and increase the versatility of the HDX-MS technique.

129 In summary, the HDX-MS workflow with integrated PNGase A deglycosylation

130 ing the intact protein on beads prior to the HDX-MS experiment.

131 A level of complexity that is inherent to HDX MS remained unaddressed, namely, various combination

132 uld be too massive to be readily amenable to HDX-MS.

133 Under typical HDX-MS conditions and using small amounts of enzyme, An-

134 pport for this hypothesis was obtained using HDX-MS which revealed protection to exchange only within

135 In this study, we utilized HDX-MS combined with structural modeling to examine the