ライフサイエンスコーパス: HGE agent

1 romyscus leucopus mice were infected with an HGE agent.

2 two PCR-positive mice, contained DNA of the HGE agent.

3 ion of antibodies to a 44-kDa protein of the HGE agent.

4 dorferi reacted positively with rP44 and the HGE agent.

5 ere primarily present on the membrane of the HGE agent.

6 ocytopenic in response to infection with the HGE agent.

7 is identical to the 16S rDNA sequence of the HGE agent.

8 ity to detect WB-confirmed antibodies to the HGE agent.

9 a single mouse was culture positive for the HGE agent.

10 were consistent with results with the whole HGE agent.

11 ologous to the P44 gene in the genome of the HGE agent.

12 igens are the outer membrane proteins of the HGE agent.

13 omonocytic lineages, were incubated with the HGE agent.

14 ultivated in HL60 cells for isolation of the HGE agent.

15 r use in testing for human antibodies to the HGE agent.

16 and C57BL/6J mice during infection with the HGE agent.

17 h a low and a high passage of in vitro-grown HGE agent.

18 and overrode the antiapoptotic effect of the HGE agent.

19 ng NADPH oxidase in human neutrophils by the HGE agent.

20 t or reverse the antiapoptotic effect of the HGE agent.

21 volved in the antiapoptotic mechanism of the HGE agent.

22 ine, blocked the antiapoptotic effect of the HGE agent.

23 E agent would be critical in survival of the HGE agent.

24 as determined to be identical to that of the HGE agent.

25 h an obligatory intracellular bacterium, the HGE agent.

26 positive blood samples were identical to the HGE agent.

27 rP44, regardless of IFA reactivity with the HGE agent.

28 jor antigenic outer membrane proteins of the HGE agent.

29 the use of whole infected cells or purified HGE agents.

30 tive to the human granulocytic ehrlichiosis (HGE) agent.

31 ical to the human granulocytic ehrlichiosis (HGE) agent.

32 We previously cloned a gene encoding HGE agent 44-kDa major outer membrane protein and design

33 rging febrile systemic disease caused by the HGE agent, an obligatory intracellular bacterium of gran

34 train of Anaplasma phagocytophila called the HGE agent, an obligatory intracellular bacterium.

35 ent strains of mice were inoculated with the HGE agent and assayed for production of polyclonal and m

36 Sera from two patients coinfected with the HGE agent and B. burgdorferi reacted positively with rP4

37 leotide primers specific for E. equi and the HGE agent and by immunocytology.

38 Some HGE agent and E. chaffeensis inclusions colocalized with

39 ecially in geographic regions where both the HGE agent and E. chaffeensis occur.

40 d the sensitivity and specificity of various HGE agent and E. equi antigens used for IFA diagnosis by

41 The HGE agent and E. equi are antigenically diverse, and int

42 The 444 Ep-ank gene of the HGE agent and E. equi isolates from northern California

43 s observed in the 16S rRNA gene sequences of HGE agent and E. equi isolates from northern California.

44 Two clones, one each from HGE agent and E. equi, that were recognized specifically

45 diverse antigenic cross-reactivities between HGE agent and Ehrlichia chaffeensis.

46 tein antigen genes, we prepared and screened HGE agent and Ehrlichia equi genomic DNA expression libr

47 lowest homology occurring between a New York HGE agent and the Swedish E. phagocytophila.

48 by immunizing BALB/c mice with the purified HGE agent and then by fusing spleen cells with myeloma c

49 Reactivity to the HGE agent and to either Coxiella burnetii, Rickettsia ri

50 onal antibodies to the 44-kDa antigen of the HGE agent and was infectious for laboratory mice.

51 philum, the human granulocytic ehrlichiosis (HGE) agent and a variant (AP-Variant 1) that has not bee

52 ting from the United States (E. equi and the HGE agent) and sequences from the European species, E. p

53 ene fragment is identical among E. equi, the HGE agent, and E. phagocytophila, with the exception of

54 tested by PCR for active infection with the HGE agent, and sera from P. leucopus mice were tested fo

55 tick, Ixodes scapularis, is a vector of the HGE agent, and the white-tailed deer is the primary host

56 ent P44-homologous proteins expressed by the HGE agent; and (vi) demonstrated existence of antibodies

57 gest that the 43- and 45-kDa proteins of the HGE agent are encoded by members of the GE MSP-2 multige

58 cells, proving that the blood stages of the HGE agent are infectious for tick cells, as are those re

59 Animals with evidence of infection with the HGE agent are widely distributed around the Minneapolis-

60 otting using purified E. chaffeensis and the HGE agent as antigens suggested that heat shock and othe

61 p44 multigene family in transmission of the HGE agent between mammals and ticks.

62 reversible upon removal of the extracellular HGE agent bound to the host cells prior to PMA stimulati

63 blot analysis, all three MAbs recognized the HGE agent but not Ehrlichia chaffeensis, Ehrlichia senne

64 nces for E. equi, E. phagocytophila, and the HGE agent by 1 or 2 nucleotides (> or = 99.1% homology)

65 and reacted with human sera positive for the HGE agent by immunofluorescence assay.

66 These were confirmed as the HGE agent by sequencing of 16S rDNA.

67 late of the human granulocytic ehrlichiosis (HGE) agent by using an indirect immunofluorescence assay

68 Furthermore, the HGE agent completely prevented O(2-) release by neutroph

69 which are not the primary host cells of the HGE agent, contributes to HGE pathogenesis and immunomod

70 ed from the p44-18 gene were detected in the HGE agent cultivated in HL-60 cells at 37 degrees C, but

71 transcribed from p44-homologous genes in the HGE agent cultivated in HL-60 cells; (ii) cloned genes c

72 To determine if the HGE agent delays the apoptosis of human peripheral blood

73 ic analysis of this fragment showed that the HGE agent-derived sequence was related to, but distinct

74 The HGE agent did not prevent O(2-) generation in human peri

75 cell culture isolation of two strains of the HGE agent directly from an infected horse and a dog and

76 HGE agent DNA and antibodies against E. equi were presen

77 PCR analyses revealed the presence of HGE agent DNA in 20 of the 190 samples (10.5%) tested.

78 uences was used to detect E. chaffeensis and HGE agent DNA in blood samples from human patients with

79 Of the seropositive patients, HGE agent DNA was detected by polymerase chain reaction

80 Infection of mice with the HGE agent does not induce diagnostically significant B.

81 The inhibition was HGE agent dose dependent, required ehrlichial contact wi

82 hese studies suggest that replication of the HGE agent during and after feeding in larvae and during

83 Thus, the 40- and 44-kDa proteins of the HGE agent elicit early strong antibody responses during

84 These data demonstrate that the HGE agent elicits a prominent IFN-gamma response in mice

85 l infection, and from mice infected with the HGE agent for up to 3 weeks.

86 These results suggest that E. equi and the HGE agent found in California are similar or identical b

87 Although much weaker than the HGE agent freshly freed from the host cells, noninfectio

88 experimental process for transmission of the HGE agent from infected mice (a reservoir model) to nymp

89 he agent of human granulocytic ehrlichiosis (HGE agent) from infected mice to larval ticks and to exa

90 es being expressed were not clustered in the HGE agent genome; (iv) estimated that a minimum copy num

91 olorado had antibodies that reacted with the HGE agent (genus GMT = 194), suggesting that enzootic cy

92 to a recombinant fusion protein based on the HGE agent groEL homolog were detected in three of three

93 es are all cross-reactive among strains, the HGE agent has a strain pleomorphism in its major antigen

94 h human granulocytic ehrlichiosis (HGE), the HGE agent has been seen only in the peripheral blood gra

95 SG3) of the human granulocytic ehrlichiosis (HGE) agent has identified a number of immunoreactive pro

96 tment within HL-60 cells: early forms of the HGE agent have a round reticular appearance while later

97 ing to the mRNAs from the genomic DNA of the HGE agent; (iii) showed that the genes being expressed w

98 fic AnkA antibodies reacted with proteins in HGE agent immunoblots, and AnkA monoclonal antibodies de

99 ion of p44-homologous genes expressed by the HGE agent in a tissue culture would assist in understand

100 to E. chaffeensis also had antibodies to the HGE agent in at least one serum sample.

101 All assays detected the HGE agent in blood during early infection, but PCR and t

102 NOS2(-/-) mice had delayed clearance of the HGE agent in comparison to control or gp91(phox-/-) mice

103 urface properties between populations of the HGE agent in different host environments.

104 nd to examine the population kinetics of the HGE agent in different stages of the tick life cycle.

105 n by the major outer membrane protein of the HGE agent in monocytes, which are not the primary host c

106 We previously isolated and cultivated the HGE agent in the promyelocytic leukemia cell line HL-60

107 In contrast, HGE agent in THP-1 or HL-60 cells induced no increase in

108 incubated with freshly freed host cell-free HGE agent in vitro.

109 vitro-grown human granulocytic ehrlichiosis (HGE) agent in horses.

110 The human granulocytic ehrlichiosis (HGE) agent in infected blood specimens remained viable d

111 both E. chaffeensis and E. sennetsu, but not HGE agent, in the acute monocytic leukemia cell line THP

112 eroxidase-positive granules were seen around HGE agent inclusions but not around E. chaffeensis inclu

113 olar-type H+-ATPase was not colocalized with HGE agent inclusions but was weakly colocalized with E.

114 or, early endosomal antigen 1, and rab5, but HGE agent inclusions were not.

115 both E. chaffeensis and E. sennetsu, but not HGE agent, inclusions in THP-1 cells or the cells of the

116 The HGE agent induced a significant delay in morphological a

117 The HGE agent induced expression of interleukin-1beta (IL-1b

118 In tick cell layers, the HGE agent induced foci of infection that caused necrotic

119 HGE agent-infected C3H/HeJ mice had an antibody response

120 HGE agent-infected C3H/HeJ mice were necropsied over 21

121 with Ehrlichia equi-infected neutrophils or HGE agent-infected cultured HL60 cells.

122 sis of a 1.6-kb DNA fragment derived from an HGE agent-infected HL60 cell culture indicated a near-co

123 lyzing the role of the major OMP antigens in HGE agent infection and for serodiagnosis.

124 However, HGE agent infection had no effect on IRP-1 binding activ

125 HGE agent infection in immunocompromised mice progresses

126 Laboratory mice are valuable models of HGE agent infection kinetics and immunity, but initial s

127 5 was more effective in protecting mice from HGE agent infection than with MAbs 5C11 and 5D13.

128 HGE-agent infection of mice induces pathologic changes s

129 tein of the human granulocytic ehrlichiosis (HGE) agent is an immunodominant antigen in human infecti

130 es, and the human granulocytic ehrlichiosis (HGE) agent is an obligatory intracellular bacterium of g

131 C11, and 5D13) were examined with five human HGE agent isolates and one tick isolate.

132 lity among serologic tests using E. equi and HGE agent isolates for diagnosis of HGE will occasionall

133 tein fractions and of whole organisms of six HGE agent isolates were similar, suggesting that 49- and

134 unties, and human granulocytic ehrlichiosis (HGE) agent isolates were obtained from Humboldt county.

135 nfection of mice with B. burgdorferi and the HGE agent modulates host immune responses, resulting in

136 ontaining a human granulocytic ehrlichiosis (HGE) agent (NCH-1 strain) were used to confirm probable

137 fluorescent antibody (IFA) testing with the HGE agent no. 13 isolate as the antigen, by nested PCR,

138 Overall, HGE agent no. 3, 6, 11, and 13, and USG isolates had sim

139 f dual infection with B. burgdorferi and the HGE agent on the course of murine Lyme arthritis and gra

140 The three HGE-agent-only IFA-positive sera reacted only with rP44,

141 l blood leukocytes (PBLs) incubated with the HGE agent or a recombinant 44-kDa major surface protein

142 Proteinase K treatment of the HGE agent or rP44 eliminated the ability to induce these

143 within 24 h of attachment, and the number of HGE agent organisms increased in larval ticks during fee

144 ng feeding in nymphs is a means by which the HGE agent overcomes inefficiencies in acquisition of inf

145 g was used to characterize the nature of the HGE agent replicative inclusions and to compare them wit

146 These results suggest that the HGE agent resides in inclusions which are neither early

147 The human granulocytic ehrlichiosis (HGE) agent resides and multiplies exclusively in cytopla

148 a variety of sources with the cell-cultured HGE agent revealed that 3 HGE agent strains (New York is

149 ies from rabbits and mice immunized with the HGE agent, sera from humans convalescent from HGE, and s

150 Rodents with HGE agent-specific antibodies were found in New York (23

151 ant fusion proteins or peptides based on the HGE agent-specific groEL homolog.

152 from the host cells, noninfectious purified HGE agent stored frozen and thawed also had antiapoptoti

153 the cell-cultured HGE agent revealed that 3 HGE agent strains (New York isolate, Wisconsin [BDS] iso

154 with 44- to 42-kDa proteins in six different HGE agent strains tested except strain 2, in which three

155 these cultures supported replication of the HGE agent, suggesting that all are potential targets of

156 version or a fourfold or higher titer to the HGE agent than to the Ehrlichia chaffeensis antigens.

157 hat binding of a protein component(s) of the HGE agent to neutrophils and subsequent cross-linking an

158 infected with a tick-derived isolate of the HGE agent (USG3).

159 The HGE agent was also isolated from the leukocyte fraction

160 ichiosis (HGE) and in mice infected with the HGE agent was characterized by using sera to probe lysat

161 HGE agent was demonstrated in mature and immature myeloi

162 ns positive by both IFA testing and PCR, the HGE agent was isolated from four (no. 2, 3, 6, and 11) a

163 ctor beta, and IL-2 mRNAs in response to the HGE agent was not remarkable.

164 integrity of or new protein synthesis by the HGE agent was not required for the inhibition; carbohydr

165 Although only the HGE agent was present in northwestern Pennsylvania, both

166 The HGE agent was quantitated by competitive PCR with blood

167 carbohydrate but not surface protein of the HGE agent was required.

168 uence identical to the published sequence of HGE agents was isolated from a patient from New York Sta

169 DNA of the human granulocytic ehrlichiosis (HGE) agent was detected in 17 of 47 mice (36.2%), but on

170 Sequence-confirmed products of the HGE agent were amplified from three individuals residing

171 Antibodies reactive to the HGE agent were detected in 142 (8.9%) of 1,602 individua

172 t 44-kDa major surface protein (rP44) of the HGE agent were examined by reverse transcription-PCR and

173 ee sera that were IFA positive only with the HGE agent were examined by Western immunoblot analysis u

174 e three cytokines in PBLs by rP44 and by the HGE agent were similar.

175 ontaining E. equi, E. phagocytophila, or the HGE agent were very similar (99.9 to 99.0% identity), an

176 ot TNF-alpha or IL-6 mRNA in response to the HGE agent, whereas monocytes expressed all three of thes

177 The human granulocytic ehrlichiosis (HGE) agent, which replicates in neutrophils, was found n

178 e inoculation with a Wisconsin strain of the HGE agent (WI-1) and nine of nine mice infected by Ixode

179 ected or have been infected naturally by the HGE agent with low-level persistent infection or frequen

180 A human granulocytic ehrlichiosis (HGE) agent with 16S rDNA sequence identical to the publi

181 MPs) of the human granulocytic ehrlichiosis (HGE) agent, with molecular sizes of 44 to 47 kDa, are im

182 set of feeding stimulated replication of the HGE agent within nymphal ticks.

183 ecific prevention of O(2-) generation by the HGE agent would be critical in survival of the HGE agent