ライフサイエンスコーパス: HP1

1 HP1 and SUV39H1/2 are repressive factors essential for H

2 HP1 binding to the CPC becomes particularly important wh

3 HP1 binds to the H3K9me3 and recruits the DNA methyltran

4 HP1 is required to recruit the DMM complex to the edges

5 HP1 proteins are central to the assembly and spread of h

6 HP1(Hsalpha)-containing heterochromatin is located near

7 HP1, EZH2, and MeCP2 in turn were associated with additi

8 ng the highly conserved hydrophobic patch 1 (HP1) and that this binding is similar to the binding of

9 umulated abundant heterochromatin protein 1 (HP1) after replicating in S phase 14.

10 s associate with hetereochromatin protein 1 (HP1) alpha- and HP1beta-containing heterochromatic foci.

11 as used to purify HETEROCHROMATIN PROTEIN 1 (HP1) and associated proteins from Neurospora.

12 We found that heterochromatin protein 1 (HP1) and G9a formed a complex at the interleukin-1beta p

13 Heterochromatin protein 1 (HP1) and HP1-ORC-associated protein (HOAP) are major pro

14 cts directly with heterochromatin protein 1 (HP1) and that this interaction is mediated by an evoluti

15 gated the role of heterochromatin protein 1 (HP1) during the DDR process.

16 Heterochromatin protein 1 (HP1) family proteins are conserved chromatin binding pro

17 The heterochromatin protein 1 (HP1) family proteins HPL-1 and HPL-2 are dispensable for

18 hosphorylation of Heterochromatin Protein 1 (HP1) family proteins regulates heterochromatin dynamics,

19 s are tethered to heterochromatin protein 1 (HP1) for coordinated chromatin movement and histone modi

20 evolving X-linked heterochromatin protein 1 (HP1) gene, HP1D2, plays a key role in the classical Pari

21 Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chr

22 The heterochromatin protein 1 (HP1) homolog Rhino binds to the H3K9me3 mark through its

23 er gene codes for heterochromatin protein 1 (HP1) in Drosophila.

24 9) and binding of heterochromatin protein 1 (HP1) in the promoter regions of these genes were substan

25 Tethered heterochromatin protein 1 (HP1) induced H3K9me3, DNA methylation, and gene silencin

26 newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding betw

27 Heterochromatin protein 1 (HP1) is a central factor in establishing and maintaining

28 Heterochromatin protein 1 (HP1) is a conserved factor critical for heterochromatin

29 ere, we show that heterochromatin protein 1 (HP1) is an essential CPC component required for full Aur

30 Heterochromatin protein 1 (HP1) is well known as a silencing protein found at peric

31 H3K9me3 marks and heterochromatin protein 1 (HP1) on the reporter locus.

32 stribution of the heterochromatin protein 1 (HP1) ortholog HPL-2 and compared its distribution to oth

33 rase Clr4 and the heterochromatin protein 1 (HP1) ortholog Swi6 are critical for RNAi, whereas RNAi s

34 identification of heterochromatin protein 1 (HP1) paved the way for a molecular dissection of this im

35 Heterochromatin protein 1 (HP1) proteins are "gatekeepers" of epigenetic gene silen

36 of the ability of heterochromatin protein 1 (HP1) proteins to spread across large regions of the geno

37 rs and recruiting heterochromatin protein 1 (HP1) proteins.

38 A heterochromatin protein 1 (HP1) tethering system was developed that generates "ecto

39 ain of heterochromatin-associated protein 1 (HP1) thereby leading to transcriptional repression and h

40 ly interacts with heterochromatin protein 1 (HP1) to promote heterochromatin stability.

41 Binding of heterochromatin protein 1 (HP1) to the histone H3 lysine 9 trimethylation (H3K9me3)

42 nteracts with the Heterochromatin Protein 1 (HP1) variant Rhino (Rhi).

43 w domain (CSD) of heterochromatin protein 1 (HP1) was recently shown to contribute to chromatin bindi

44 he interaction of heterochromatin protein 1 (HP1) with chromatin.

45 Heterochromatin protein 1 (HP1), a highly conserved non-histone chromosomal protein

46 ), the homolog of heterochromatin protein 1 (HP1), down-regulates the UPR in the intestine.

47 on complex (ORC), heterochromatin protein 1 (HP1), histone H3 trimethyl K9 (H3 K9me3), and members of

48 binding proteins heterochromatin protein 1 (HP1), polycomb protein complex 1 (PRC1) and methyl CpG b

49 ly antagonized by heterochromatin protein 1 (HP1), the code reader for histone H3 lysine 9 methylatio

50 romatin spread is heterochromatin protein 1 (HP1), which recognizes H3K9-methylated chromatin, oligom

51 is facilitated by heterochromatin protein 1 (HP1), which serves as an adapter protein.

52 ransferase DIM-5, Heterochromatin Protein 1 (HP1), which specifically binds to the product of DIM-5 (

53 swiftly mobilize heterochromatin protein 1 (HP1)-beta (also called CBX1), a chromatin factor bound t

54 cruiting multiple heterochromatin protein 1 (HP1)-containing complexes that deacetylate histones and

55 le association to heterochromatin protein 1 (HP1).

56 logue of metazoan heterochromatin protein 1 (HP1).

57 s binding partner heterochromatin protein 1 (HP1).

58 binding site for heterochromatin protein 1 (HP1).

59 The role of Heterochromatin Protein-1 (HP1) during mitosis has been controversial.

60 Mutants in genes encoding HPL-2/HP1, LIN-13, LIN-61, LET-418/Mi-2, and H3K9me2 histone m

61 how that DIM-2-dependent RIP requires DIM-5, HP1, and other known heterochromatin factors, implying a

62 ther, the present structural analysis of 7SK HP1 highlights an original mechanism of swapping bases,

63 nding site embedded in the 5-hairpin of 7SK (HP1) encompasses a short signature sequence, a GAUC repe

64 e-specific nuclear Argonaute HRDE1/WAGO-9, a HP1 ortholog HPL-2, and two putative histone methyltrans

65 that inhibiting GRPR signaling, or ablating HP1(Hsbeta) expression, increases colon cancer cell inva

66 Although HP1 links H3K9me3 to DNA methylation, it also serves to

67 mulated relative binding free energies among HP1 chromodomain-H3 tail complexes differing at position

68 or the binding of a methylated H3 tail by an HP1 chromodomain but indicate that the effect from an el

69 novo centromeres in C. elegans embryos in an HP1-independent manner and suggest that, rather than bei

70 his issue, Klattenhoff et al. report that an HP1 family protein, Rhino, is required for piRNA generat

71 s nucleate at the core but require DIM-5 and HP1 for spreading.

72 lear factors such as lamin A/C, lamin B, and HP1.

73 The multivalent engagement with DNA and HP1 results in a nucleosome binding circuit in which KDM

74 of the heterochromatin markers (H3K9me3 and HP1-gamma) and a concomitant increase in the double-stra

75 of ATRX to interact with H3K9me3 histone and HP1.

76 o study the distribution of HipHop, HOAP and HP1 using chromatin immunoprecipitation (ChIP).

77 Heterochromatin protein 1 (HP1) and HP1-ORC-associated protein (HOAP) are major protein comp

78 H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by KDM2A.

79 and melanoma, respectively, HP1(Hsalpha) and HP1(Hsbeta) have been shown to modulate the aggressivene

80 st in humans: HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma).

81 ctural studies show that both HP1-INCENP and HP1-Sgo1 interactions require the binding of the HP1 chr

82 tion of chromatin binding for both KDM2A and HP1 that is modulated by DNA- and H3K9-methylation, and

83 at mediates direct binding between KDM2A and HP1.

84 haracterized by histone H3K9 methylation and HP1 protein binding, silences the underlying DNA and inf

85 contain histone H3 lysine 9 methylation and HP1.

86 Although H3K9 methyltransferases and HP1 are necessary for proper heterochromatin structure,

87 at recognition of methylated nucleosomes and HP1 spread on chromatin are structurally coupled and imp

88 to heterochromatin, suggesting that ORC and HP1 proteins are mutually required for each other to bin

89 as insulators to block the spread of Sir and HP1 mediated silencing while in metazoans most insulator

90 ated the levels of unphosphorylated STAT and HP1 [encoded by Su(var)205] in Drosophila and examined t

91 The HP64-f/HP64-r for ureA and HP1/HP2 for 16S rRNA individually had sensitivities and

92 echanism of regulating the expression of any HP1 isoform in any context has not yet been identified.

93 These include key components such as HP1, Trithorax-like (GAGA factor), Su(var)3-9, Brahma, M

94 AP56 colocalizes with the cluster-associated HP1 variant Rhino, that nuage granules containing Vasa l

95 a minimal fold similar to that of bacterial HP1 integrase and defines structural elements conserved

96 d for verification of an interaction between HP1 and DIM-2 in vivo by Co-IP assays on proteins expres

97 rochromatin by bridging interactions between HP1 and histone deacetylase complexes.

98 viously unrecognized but direct link between HP1 and CPC localization in the centromere and illustrat

99 and illustrate the critical role of borealin-HP1 interaction in orchestrating an accurate cell divisi

100 n the midzone is independent of the borealin-HP1 interaction, demonstrating the spatial requirement o

101 This borealin-HP1 interaction recruits the CPC to the centromere and g

102 emical and structural studies show that both HP1-INCENP and HP1-Sgo1 interactions require the binding

103 lly, Epe1 is recruited to heterochromatin by HP1 silencing factors that are distributed throughout he

104 methylation (H3K9me) and its recognition by HP1 proteins are necessary for pericentromeric heterochr

105 methylation (H3K9Me) and its recognition by HP1 proteins.

106 1gamma), that binds to a conserved canonical HP1-binding motif, PXVXL, in the C-terminal domain of TI

107 Conserved chromosomal HP1 proteins capable of binding to histone H3 methylated

108 Clearly, HP1 function is altered by context, and potentially by p

109 results were simulated by the numerical code HP1 (Hydrus-PhreeqC) with the DLVO theory, extended coll

110 cycle 15, satellites clustered in a compact HP1-positive mass, but replication occurred at decondens

111 We also suggest that compromising HP1 expression could promote tumorigenesis by impairing

112 including an acidic stretch and a consensus HP1-binding motif.

113 Consequently, HP1 has many important roles in chromatin architecture a

114 where they also interact with the conserved HP1 protein.

115 otif within CCM2 binds the highly conserved "HP1" pocket of the CCM3 focal adhesion targeting (FAT) h

116 ipts and SarA protein than the corresponding HP1 stem and the HP2 stem and loop mutations, leading to

117 ed HDAC4 nuclear export, H3K9 demethylation, HP1 dissociation from the promoter region, and activatio

118 Collectively our results demonstrate HP1-alpha oligomerization is critical to the maintenance

119 Depleting HP1 reduced recruitment of BRCA1 to DSBs and caused defe

120 Depleting HP1-gamma up-regulated proliferation-promoting genes in

121 In contrast, depleting HP1 from cells did not affect the non-homologous end-joi

122 How different HP1 proteins, which are highly conserved, perform differ

123 The Drosophila HP1 homolog Rhino is required for germline piRNA product

124 entiating the interactions of the Drosophila HP1 paralogs.

125 me3 histone mark and its downstream effector HP1.

126 d previous in vitro findings that endogenous HP1 preferentially binds H3K9me3.

127 vivo interactions with the chromatin factors HP1 and SMC3 and the cofactors Sin3A and YB-1.

128 Drosophila genomes possess at least five HP1 paralogs that have significantly different roles, ra

129 echanism is not clarified yet especially for HP1.

130 our results point to multiple functions for HP1 in different cell types to maintain ER homeostasis.

131 ively, these studies reveal a novel role for HP1 as a cofactor in tumor suppression, expand our mecha

132 re recent studies have discovered a role for HP1 in numerous processes including, surprisingly, euchr

133 al. reveal a new H3K9me-independent role for HP1 in the DNA damage response, which is distinct from t

134 H enzymes, which provides a docking site for HP1 proteins, therefore mediating heterochromatin compac

135 analyses suggest that among other functions, HP1 proteins associate with chromatin-modifying factors

136 alter the hydrogen-bonding regime in the H3-HP1 complex.

137 themselves provide a binary switch in the H3-HP1 system, but arginine-phosphoserine interactions, whi

138 ector backbone localizing within an H3K9me3, HP1-enriched core.

139 y 23 A between the helix HP1b on the hairpin HP1 and the transmembrane helices TM7 and TM8, using the

140 Species that have HP1 proteins possess multiple paralogs that perform non-

141 laying a structural role in heterochromatin, HP1 proteins can have both an activating as well as repr

142 In addition to its role in heterochromatin, HP1 proteins have been shown to function in transcriptio

143 Unexpectedly, a second hit, HIPP1 (HP1 and insulator partner protein-1) (CG3680), is strong

144 How HP1 proteins assemble on methylated nucleosomal template

145 multidisciplinary approach to determine how HP1(Hsalpha)-nucleosome interactions contribute to the s

146 rent isoforms exist in humans: HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma).

147 demonstrate that the CSDs of all three human HP1 homologs have comparable affinities to the PXXVXL mo

148 ch three different isoforms exist in humans: HP1(Hsalpha), HP1(Hsbeta), and HP1(Hsgamma).

149 Mechanistic studies implicate HP1 family proteins as 'hub proteins,' able to interact

150 ontrast, INCENP or Sgo1 mutants deficient in HP1 binding fail to localize to centromeres in interphas

151 Consistently, a Sgo1 mutant deficient in HP1 binding is functional in centromeric cohesion protec

152 A KDM2A mutant deficient in HP1-binding is inactive in an in vivo overexpression ass

153 ns of HP1(Hsalpha) play an essential role in HP1(Hsalpha)-nucleosome interactions, whereas the hinge

154 umulation of repressive complexes, including HP1, the NuRD complex, H2A.Z and histone methyltransfera

155 KLF11-regulated cancer genes, by inhibiting HP1-SUV39H1 recruitment, decreasing H3K9me3, while incre

156 Biologically, impairment of KLF11-mediated HP1-HMT recruitment abolishes tumor suppression, providi

157 e is required for histone H3-K9 methylation, HP1 localization, and heterochromatin-mediated gene sile

158 Neutralizing HP1 function dysregulated the formation of DERE-involved

159 ently show that the helical hairpin HP2, not HP1, serves as an intracellular gate (in addition to its

160 unctions of HP1, we sought to identify novel HP1-interacting proteins.

161 P distribution was altered in the absence of HP1, the chromodomain protein that binds to H3K9me3.

162 We find from fluctuation analysis of HP1-alpha dynamics that this isoform exists as a dimer a

163 igating the methylation dependent binding of HP1 to full length histone H3 monomethylated on K9 (H3K9

164 ylated K9 H3 peptide in the aromatic cage of HP1 is only slightly affected by S10 phosphorylation, be

165 The chromodomain (CD) of HP1 proteins specifically recognizes the methyl mark on

166 oci marker and found that depleting cells of HP1 caused genotoxic stress, a delay in the repair of DS

167 Depletion of HP1, SUV39, SETDB1 or BRCA1 confer identical phenotypes.

168 ressing S473A was alleviated by depletion of HP1-beta, suggesting that phosphorylation of KAP-1 on Se

169 ve been elucidated, the molecular details of HP1 binding to H3K9me3 nucleosomes are unknown.

170 The chromoshadow domain of HP1 proteins promotes homodimerization, but this alone c

171 al borealin with the chromo shadow domain of HP1.

172 The chromo and chromo shadow domains of HP1(Hsalpha) play an essential role in HP1(Hsalpha)-nucl

173 Elimination of HP1, which "reads" H3K9me3, also caused major changes in

174 insights into the transcription functions of HP1, we sought to identify novel HP1-interacting protein

175 Each of the three human homologs of HP1 includes a chromoshadow domain (CSD).

176 Partial inhibition of HP1 family proteins accelerates the acquisition of segre

177 From inhibition of HP1-alpha homo-oligomerization we find the slow turn rat

178 We suggest that the dynamic interaction of HP1 with chromatin and other DDR factors could determine

179 t eukaryotes have at least three isoforms of HP1 that play differential roles in heterochromatin and

180 Knockdown of HP1 resulted in a decreased Dnmt3a/b binding, sustained

181 Because loss of HP1 caused redistribution of H3K27me2/3, but not H3K9me3

182 Abe et al. (2016), suggest that the means of HP1 localization and its function at inner centromeres a

183 Electron microscopy of HP1(Hsalpha)-associated nucleosomal arrays showed that H

184 AP-1 on Ser-473 promotes the mobilization of HP1-beta from heterochromatin and subsequent DNA repair.

185 dings that support the liquid-like nature of HP1 domains and discuss their functional implications in

186 Instead, phosphorylation of HP1-beta on amino acid Thr 51 accompanies mobilization,

187 hanism acts parallel with phosphorylation of HP1/Swi6 by CK2 to restrict Epe1.

188 Remarkably, a reduced proportion of HP1 bound to CPC is widespread in cancers, which causes

189 fect on total H3K9me3 levels, recruitment of HP1-gamma to promoters was lost.

190 on, demonstrating the spatial requirement of HP1 in CPC localization to the centromere.

191 ted knockdown of ASF/SF2 caused retention of HP1 proteins on mitotic chromatin.

192 In contrast, the role of HP1 in colon cancer has not been elucidated, and a mecha

193 Differential sedimentation of HP1(Hsalpha)-associated nucleosomal arrays showed that H

194 The present crystal structure of HP1 shows a remarkably straight helical stack involving

195 Notably, all three subtypes of HP1 seemed to be almost equally important for these DDR

196 the diversity and incredible versatility of HP1 proteins in organizing and protecting eukaryotic gen

197 Asp-394 on TM8 and Arg-276 on HP1 emerge as key residues that promote the reorientatio

198 calization with paxillin in cells is lost on HP1 mutation.

199 to constitutive heterochromatin in DCDC- or HP1-deficient mutants, and introduction of an H3K27 miss

200 Surprisingly, loss of either H3K9me3 or HP1 had only mild effects on heterochromatin compaction,

201 shes nucleolar associations, whereas PCNA or HP1 interaction sites within p150 are not required for t

202 " in higher eukaryotes (e.g., by Polycomb or HP1) follows similar rules [4, 5] and note where such ef

203 S boundary, Orc3 facilitates assembly of ORC/HP1 proteins to chromatin.

204 Although HPL-2, like other HP1 orthologs, binds H3K9me peptides in vitro, the distr

205 (Fluorescent proteins, actin, tubulin, p53, HP1).

206 ighly conserved FAK-like hydrophobic pocket (HP1) abrogates CCM3-CCM2 interaction.

207 ere we show that binding of the key S. pombe HP1 protein, Swi6, to methylated nucleosomes drives a sw

208 Using the S. pombe HP1 protein, Swi6, we show that recognition of H3K9-meth

209 The fission yeast Schizosaccharomyces pombe HP1 family members Chp2 and Swi6 are important for heter

210 re, we use the two Schizosaccharomyces pombe HP1 paralogs, Swi6 and Chp2, as model systems to compare

211 ne such property, late replication, precedes HP1 recruitment, is under the control of zygotic transcr

212 gest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centro

213 In contrast to previous proposals, HP1 can neither initiate nor accommodate neurotransmitte

214 e IIbeta, heterochromatin-associated protein HP1 or CTCF, were involved in this interaction.

215 r p21 expression and heterochromatin protein HP1-gamma phosphorylation.

216 d pRb interacts with heterochromatin protein HP1.

217 h the architectural heterochromatin proteins HP1, DEK1, and ATRx, and was required for their localiza

218 A-like protein and two hypothetical proteins HP1 and HP2 that were strongly linked to a novel Se util

219 sed in transport studies (i.e., PSA-HM1, PSA-HP1, PSA-HS2, and H3/49).

220 Two phages PSA-HP1, PSA-HS2 (Podoviridae and Siphoviridae) exhibited si

221 Remarkably, the PTVML HP1-binding site is embedded in the recently identified

222 A PXVXL HP1-interacting domain identified at position 487-491 of

223 iously reported primer pairs (HP64-f/HP64-r, HP1/HP2, EHC-U/EHC-L, VAG-F/VAG-R, and ICT37/ICT38) had

224 leosome arrangement synergistically regulate HP1 function.

225 d chromatin restructuring via Chk2-regulated HP1-beta exchange from heterochromatin, promoting DNA re

226 P/GRPR signaling specifically down-regulates HP1(Hsbeta) expression and that inhibiting GRPR signalin

227 d Thr 51 accompanies mobilization, releasing HP1-beta from chromatin by disrupting hydrogen bonds tha

228 terochromatin, compaction, late replication, HP1 binding, and aggregation at the chromocenter, in suc

229 In breast cancer and melanoma, respectively, HP1(Hsalpha) and HP1(Hsbeta) have been shown to modulate

230 Although initial studies revealed HP1's key role in heterochromatin maintenance and functi

231 on of compacted chromatin in phase-separated HP1 droplets, which are dissolved or formed by specific

232 le for SRY by tethering the KAP1-NuRD-SETDB1-HP1 silencing machinery to repress SRY targets.

233 N mice were challenged with H. pylori strain HP1, expressing Le(x) and Le(y), we found that bacterial

234 re are two putative hairpin-loop structures, HP1 and HP2.

235 y active heterochromatin, whereas Chp2/Swi6 (HP1 homologs) are recruited during the inactive state.

236 deleting the heterochromatin proteins Swi6 (HP1 ortholog) and Clr4 (Suv39 family of histone methyltr

237 2 and Clr3 and the chromodomain protein Swi6(HP1) are required for H3K9me spreading from nucleation s

238 ers transcripts to centromeres, whereas Swi6(HP1)-bound transcripts are evicted from chromatin and de

239 iscovered that RNAi and Sir2 along with Swi6(HP1) operate in two independent pathways to maintain het

240 sion is promoted by the dissociation of Swi6/HP1 and cohesin Rad21 from telomeres, whereas disjunctio

241 ith the heterochromatin binding protein Swi6/HP1.

242 non, increasing the specificity of targeting HP1-HMT complexes to gene promoters.

243 establishment of H3K9me3 induced by tethered HP1.

244 Our findings demonstrate that HP1(Hsalpha)-nucleosome interactions cause chromatin con

245 suppression, providing direct evidence that HP1-HMTs act in a sequence-specific manner to achieve th

246 r promote chromatin compaction, we find that HP1-alpha dimers spatially redistribute to favor fast (5

247 Unexpectedly, we also found that HP1 proteins interact with ASF/SF2 in mitotic cells.

248 These results indicate that HP1 is an essential modulator for CPC function and ident

249 the distribution of H3K27me, indicating that HP1 is important for normal localization of facultative

250 mical and proteomic approaches revealed that HP1 interacts with the histone chaperone complex FACT (f

251 Using this system, we show that HP1 dimerization and the PxVxL interaction platform form

252 We show that HP1(Hsalpha) preferentially binds histone H3K9Me3-contai

253 a)-associated nucleosomal arrays showed that HP1(Hsalpha) caused nucleosome associations within an ar

254 a)-associated nucleosomal arrays showed that HP1(Hsalpha) promotes interactions between arrays.

255 Therefore, our results suggest that HP1 binding by INCENP or Sgo1 is dispensable for centrom

256 lobal histone deacetylation and suggest that HP1-associated histone chaperone promotes nucleosome occ

257 The HP1 C-terminal extension enhances the affinity, as does

258 The HP1 loop mutant also exhibited less biofilm formation th

259 histone H3K9 methyltransferase Clr4 and the HP1 proteins Swi6 and Chp2, as well as the two catalytic

260 lse positive; both the HP64-f/HP64-r and the HP1/HP2 sets produced false positives with saliva.

261 Ds) of the H3K9 methylase Suv39/Clr4 and the HP1/Swi6 protein.

262 Surprisingly, either the HP1 chromo domain or the chromo shadow domain alone is s

263 methylated nucleosomal templates and how the HP1-nucleosome complex achieves functional versatility r

264 n two homologous chromodomain modules in the HP1 and Polycomb proteins exhibit discriminatory binding

265 ing a cell line with a point mutation in the HP1 binding domain of TRIM28, that interaction with HP1

266 cription and augment RNAII expression in the HP1 loop mutant.

267 characterized a phosphorylation site in the HP1-binding domain of KAP-1, Ser-473, which is phosphory

268 raphically defined complex that involves the HP1 chromodomain and an H3 tail peptide.

269 ge in the context of wild-type levels of the HP1 and Mod(mdg4) proteins might be part of an adaptive

270 ction platform formed by dimerization of the HP1 chromo shadow domain are necessary for spreading to

271 Sgo1 interactions require the binding of the HP1 chromo shadow domain to PXVXL/I motifs in INCENP or

272 in AWC, we observe increased binding of the HP1 homolog HPL-2 at the odr-1 locus in AWC and reduced

273 Transversion mutation of the HP1 loop produced a smaller amount of sarA P3 and P2 tra

274 Here we probe how oligomerisation of the HP1-alpha isoform modulates interaction with chromatin,

275 also displayed defective mobilization of the HP1-beta chromodomain protein.

276 ously reported that dephosphorylation of the HP1-like protein Pdd1p is required for the formation of

277 We show that the HP1 homolog Rhino is required for nuage organization, tr

278 in zebrafish embryos demonstrating that the HP1 interaction is essential for KDM2A function.

279 We further demonstrate that the HP1-binding site in TIN2 is required for sister telomere

280 3-methylnorleucine 9 in their binding to the HP1 clearly reveals the importance of the charge indepen

281 oincidentally, the Suv39/Clr4 CD, unlike the HP1/Swi6 CD, has been shown to prefer the trimethyl stat

282 vidual tyrosines to cation binding using the HP1 chromodomain as a model system.

283 we isolated proteins that interact with the HP1/ORC-associated protein (HOAP) capping protein, and i

284 rochromatin and the tunable dynamics of this HP1 isoform.

285 ruited to H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by

286 hering the corresponding enzyme complexes to HP1-coated chromatin, thereby placing them in proximity

287 Direct binding of both Orc1 and Orc3 to HP1 suggests that, after the degradation of Orc1 at the

288 lmost all key NSC genes are switched off via HP1-mediated repression.

289 eveal for the first time a mechanism whereby HP1 is recruited to promoters by a well characterized Kr

290 These data support a model whereby HP1 takes part in multiple mechanisms of silencing and s

291 urrent knowledge supports a paradigm whereby HP1 proteins achieve repression by binding to H3K9me mar

292 spread in vivo by reducing competition with HP1 proteins for the more prevalent dimethyl state.

293 ransferase DIM-2 was found in a complex with HP1.

294 in heterochromatin through cooperation with HP1 proteins.

295 ding domain of TRIM28, that interaction with HP1 is absolutely required for the PBS-dependent restric

296 state of ACMs through their interaction with HP1-gamma to direct heterochromatin formation and silenc

297 rs1 directly and specifically interacts with HP1/Swi6.

298 f KAP1, a master co-repressor, together with HP1 and the SETDB1 histone methyltransferase, which resu

299 e repeats, suggesting that ORC together with HP1 proteins may be involved in organizing higher-order

300 In fission yeast, HP1 proteins Chp2 and Swi6, which bind to methylated his