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1 HTH binds to DNA as part of a HTH/Hox/EXD trimeric compl
2 HTH is a member of a small gene family in Arabidopsis an
3 HTH-POA slice ATP levels remained steady for 2, 4 and 6
7 e sulfenic acid oxidation of a cysteine in a HTH-motif leads to differential effects on gene expressi
11 d wild type DNA-binding activity, an altered HTH-3 domain resulted in reduced binding to the three pr
13 educed binding to the three promoters and an HTH-4 mutant was devoid of detectable binding activity.
14 edicted to be in the recognition helix of an HTH motif, was challenged with altered att sites created
16 y diversity for DNA-binding proteins with an HTH motif, and much smaller diversity for those with an
17 ese variables detect 78% of proteins with an HTH motif, which is a substantial improvement over previ
19 tational analyses showed that both CMD-1 and HTH-4 are also necessary for activation of the promoter
20 ae subsp. dysgalactiae showed that CMD-1 and HTH-4 are critical for transcriptional activation in thi
21 Plasmid-encoded expression of the HTH-3 and HTH-4 alleles from a constitutive promoter (Pspac) in th
23 o contain two DNA-binding domains (HTH-3 and HTH-4) that are required for direct activation of the Mg
28 sequences are much more similar to bacterial HTH domains than to eukaryotic ones, such as the PAIRED,
29 ytR deletion mutants lacking the DNA binding HTH domain, with tandem CRP dimers bound to either udpP
31 by itself, so it may be that the DNA-binding HTH motif becomes rigidly defined only when FlhD forms a
34 ssay using Hsp27 promoter revealed that both HTH domains contribute in a cooperative manner to the tr
35 there is a reciprocal repression exerted by HTH on these and other DPP and WG downstream targets tha
38 MBF1 is the only highly conserved, classical HTH domain that is vertically inherited in all archaea a
41 ntral catalytic domain is intact, PspF delta HTH at physiological concentration cannot activate psp e
42 , multicopy-plasmid-borne PspF or PspF delta HTH overcomes repression of the psp operon mediated by t
43 877) encodes a truncated protein (PspF delta HTH) that lacks the DNA-binding helix-turn-helix (HTH) m
45 appears to contain two DNA-binding domains (HTH-3 and HTH-4) that are required for direct activation
47 helix 6, the recognition helices of the dual HTH motifs, are important to DNA binding and transcripti
48 helix (helix E) of the helix D-turn-helix E (HTH) DNA-binding domain of the three holo-repressors.
51 g motifs, and it has been proposed that each HTH motif recognizes a highly conserved recognition elem
53 ate that residues L198 and T199 in the first HTH motif of ExsA contact the guanine in the GnC sequenc
54 haS-CTD substitutions clustered in the first HTH motif, and suggested that l-rhamnose induces improve
57 protein structures that include a functional HTH motif and have no apparent sequence similarity to ea
58 inst a previously proposed helix-turn-helix (HTH) binding site located in another region of the monom
59 the marginally stable lacI helix-turn-helix (HTH) DNA binding domain using circular dichroism and wit
60 links LOV regulation to a helix-turn-helix (HTH) DNA binding domain, we demonstrated that the LOV do
62 hat only one of Rob's dual helix-turn-helix (HTH) DNA binding motifs binds a recognition element of t
64 terminal half of EspR is a helix-turn-helix (HTH) DNA-binding domain and the carboxy terminus consist
65 of proteins containing the helix-turn-helix (HTH) DNA-binding domains whose sequences are much more s
66 alpha-helices, including a helix-turn-helix (HTH) DNA-binding motif formed by helices 3 and 4, and ca
67 that lack one helix of the helix-turn-helix (HTH) DNA-binding motif or the entire motif retain residu
68 ne regulatory protein with helix-turn-helix (HTH) DNA-binding motif, GalS contains a functional opera
70 ily, SoxS has two putative helix-turn-helix (HTH) DNA-binding motifs, and it has been proposed that e
72 that contains a classical helix-turn-helix (HTH) domain and can be assigned to the Xre family of tra
73 It is thought to have a helix-turn-helix (HTH) domain at the N-terminus and possesses two CXXC mot
74 ng subfamily of the winged helix-turn-helix (HTH) domain family whose members share a remarkable abil
76 of DNA is recognized by a helix-turn-helix (HTH) motif and the adjacent minor grooves are contacted
77 r37/Pro39 of the repressor helix-turn-helix (HTH) motif and the methyl groups of specific thymine bas
78 is of peptides with hybrid helix-turn-helix (HTH) motif and their conformational analysis (NMR, MD, a
80 inding domain folds into a helix-turn-helix (HTH) motif flanked on either side by "wings" of polypept
84 ed mutagenesis of the AmpR helix-turn-helix (HTH) motif identified residues critical for binding and
86 sembling the characterized helix-turn-helix (HTH) motif involved in DNA recognition by many phage and
87 bound to site 1, the first helix-turn-helix (HTH) motif of ExsA interacts with the conserved GnC sequ
88 ructural similarity to the helix-turn-helix (HTH) motif of the lambda repressor DNA-binding domain.
89 binding domain and a 57-aa helix-turn-helix (HTH) motif that is structurally related to the transcrip
90 ns, CodY appears to have a helix-turn-helix (HTH) motif thought to be critical for interaction with D
98 DNA using small contiguous helix-turn-helix (HTH) motifs comprise a significant number of all DNA-bin
101 bp target sites using two helix-turn-helix (HTH) motifs that are both located in its C-terminal doma
102 he AraC family contain two helix-turn-helix (HTH) motifs that contact two segments of the DNA major g
103 metries of the EF-hand and helix-turn-helix (HTH) motifs was investigated by NMR and CD spectroscopy
104 domain (PD), which has two helix-turn-helix (HTH) motifs, and the homeodomain (HD), made up from anot
106 that the marginally stable helix-turn-helix (HTH) recognition element is greatly stabilized by operat
107 stitutions in two putative helix-turn-helix (HTH) recognition helices that caused differential promot
109 n three structural motifs: helix-turn-helix (HTH), helix-hairpin-helix (HhH) and helix-loop-helix (HL
110 e that K52 residues within helix-turn-helix (HTH), K80, R82 and R88 (in the wing) and L105 (in the al
111 gion (aa 418-530) with two helix-turn-helix (HTH)-like domains, and binds to a heat shock element (HS
116 nt alleles of the organ fusion gene HOTHEAD (HTH) can inherit allele-specific DNA sequence informatio
117 lar cloning and characterization of HOTHEAD (HTH), a gene required to limit cellular interactions bet
118 hat loss-of-function alleles of the HOTHEAD (HTH) gene in Arabidopsis thaliana are genetically unstab
119 ta-gamma-alpha-gamma-alpha") showed a hybrid HTH with "11/9/11/9/11/16/9/12/10" H-bonding, while the
120 ortunity for the design and study of "hybrid HTH" motifs with more than one kind of helical structure
123 d to show that MelR residue 273, which is in HTH 2, interacts with basepair 13 of each target site.
124 th 10-6 M TAM led to decreased ATP levels in HTH (but not POA), and a 4-h incubation with 10-8 M led
125 tion with 10-6 M FLU decreased ATP levels in HTH (but not POA), while incubation with E2 did not affe
127 is that both TAM and FLU alter ATP levels in HTH slices via calmodulin- or calcium-mediated processes
128 ata showed that, following a 2-h incubation, HTH and POA slices had comparable ATP levels to hippocam
129 lytical ultracentrifugation, that individual HTH motifs of the Bacillus phage SF6 small terminase bin
130 yonic leucine zipper kinase (MELK) inhibitor HTH-01-091, CRISPR/Cas9-mediated MELK knockout, a novel
139 om the surface exposed in unfolding the lacI HTH and from the folded surface of HEWL than expected fr
140 he observed decrease in m-value for the lacI HTH with increasing temperature, together with the obser
142 n the orientation of binding of the two MelR HTH motifs, and the juxtaposition of the different bound
145 6 M TAM decreased ATP levels in POA (but not HTH) slices, while the exposure of slices to the lower c
146 led to increased ATP levels in POA (but not HTH); a 15-min exposure to 10-6 M TAM decreased ATP leve
147 we investigate how such a circular array of HTH motifs enables specific recognition of the viral gen
150 re is also a post-transcriptional control of HTH by exd: exd activity is required for the apparent st
152 e show that a conserved N-terminal domain of HTH directly binds to EXD in vitro, and is sufficient to
156 pha/beta-octapeptides showed the presence of HTH structures with bifurcated 11/15-H-bonded turn.
163 amino acyl residues in or near the putative HTH region of GerE and potentially other members of the
168 , Y250, T252, and R257 located in the second HTH motif contribute to the recognition of the TGnnA seq
169 clustered at a single residue in the second HTH motif, at a position consistent with improved RNAP c
171 try 1CMK , and a helix-turn-helix structure (HTH conformation), similar to PDB entry 4DFX , which sho
172 symmetric dimer with extended amino-terminal HTH (helix-turn-helix) domains that contact A-boxes.
173 N-terminal region (NTR), and its C-terminal HTH (helix-turn-helix) domain is also unique in DNA reco
174 main, as well as the AAA+ and the C-terminal HTH dom-ains of ZraR can be fitted into the reconstructi
175 d cnfR (patB) whose product has a C-terminal HTH domain and an N-terminal ferredoxin-like domain.
176 at to site 2 and site 2' with the C-terminal HTH located towards the promoter-proximal end of each si
177 nition element 1 (RE1), while the C-terminal HTH motif interacts with the less conserved recognition
184 s our model for DNA-bound MelR suggests that HTH 2 must be adjacent to the melAB promoter -35 element
188 rations can be modelled for the AAA+ and the HTH domains, suggesting the nature of the conformational
192 sequences in the DNA sequences encoding the HTH motif; none of them, except MerR, are known to be au
195 (dT)9, (iii) provide data that implicate the HTH motif in dsDNA binding, and (iv) show that BRCA2 sti
196 with single amino acid substitutions in the HTH and non-specific DNA-binding by the wild-type protei
197 r, mutating a key DNA binding residue in the HTH homeodomain abolishes many of its in vivo functions.
198 lanine substitutions at two positions in the HTH region of GerE on binding to wild-type or mutant tar
200 e DNA-binding domains, which incorporate the HTH motif, the second library included shorter models of
201 d that the LOV domain binds and inhibits the HTH domain in the dark, releasing these interactions upo
202 c TFIIB and TFIIE-alpha possess forms of the HTH domain that are divergent in sequence, their archaea
204 nge and subsequent proper positioning of the HTH domains in the major groove of the two half sites of
209 er with the recognition alpha-helices of the HTH motifs of each monomer separated by a distance of 34
210 st cell, GSH induces the correct fold of the HTH motifs, thus priming the PrfA protein for DNA intera
212 rophilic residues in the second helix of the HTH yields a stable, dimeric form of NtrC defective in D
214 cterial-type transcriptional regulators, the HTH domain is conserved in archaeal and eukaryotic core
215 Cysteine alkylation sterically shifts the HTH recognition helix to evidently mechanistically coupl
217 ence discrimination, which suggests that the HTH motif binds DNA as a folded domain and thus cleaves
218 trary single-site substrate suggest that the HTH motif contacts DNA similarly to how certain HTH prot
219 -containing proteins contact DNA through the HTH and hairpin structures, but only extended-ARID prote
221 possess an unusual architecture in which the HTH motifs are displayed in a ring, distinct from the cl
223 facilitated by an arginine finger within the HTH motif to stabilize the extrahelical O(6)-alkylguanin
234 romoter's robbox, we determined that the two HTH motifs each bind a recognition element in vivo.
237 ates DNA binding, which indicates an unusual HTH-DNA interaction mode in which the N termini of the r
238 the addition of another branch to the winged HTH protein family and could contribute to our understan
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