ライフサイエンスコーパス: His

1 His 212 is located at the fulcrum of these conformationa

2 His achievements included proving the occurrence of benz

3 His AFP level declined rapidly after resection, and comp

4 His article spurred theoretical development in epidemiol

5 His bilateral visual acuity was no light perception (NLP

6 His boosting the image of the scalpel wielder helped sha

7 His brother with low-to-moderate-affinity IgG1 and IgG3

8 His cancer remains in complete remission 3 years later w

9 His case was reviewed by several specialists to develop

10 His condition was complicated by pulmonary emboli and lo

11 His cure is attributed to leukemia treatment that includ

12 His current work focuses on nematode-bacterial interacti

13 His Eastern Cooperative Oncology Group performance statu

14 His examination was consistent with cerebellar and upper

15 His family noted word-finding difficulty, especially wit

16 His father had childhood type 1 diabetes in poor glycemi

17 His height was at the 25th percentile for chronological

18 His inferences were bold, because no close ethnographic

19 His influence on macrocyclic and supramolecular chemistr

20 His initial abdominal CT showed no signs of posstraumati

21 His interests (always primarily arboviruses but now also

22 His laboratory evaluation showed normal blood cell count

23 His markedly elevated inhibin B was unable to inhibit FS

24 His medical history included prostate cancer, hypertensi

25 His medical history was significant for hyperlipidemia a

26 His most recent electrocardiogram was profoundly changed

27 His mother had asthma, but there was no other family his

28 His mother has Sickle cell anaemia (Hb SS) and his fathe

29 His name will always be associated with the discovery of

30 His physician noted a mildly distended abdomen that was

31 His postoperative antibody profile was found to be subst

32 His previous treatment included orchiectomy, which revea

33 His professional life coincided with the early developme

34 His proposal for comparative semiotic analyses of how vi

35 His proposal to repeal and replace the Affordable Care A

36 His prostate-specific antigen (PSA) level was 7.1 ng/mL.

37 His PSA was monitored every 6 months and gradually incre

38 His research talent at du Pont earned him an appointment

39 His serum AFP level was elevated at 47 ng/mL.

40 His serum alphafetoprotein level normalised within 2 mon

41 His sister had a history of multiple visual abnormalitie

42 His social history was notable for a 20-pack-year smokin

43 His studies of the relationships between physical activi

44 His temperature was 38.1 degrees C, and physical examina

45 His theories, however, did not arise de novo, being stro

46 His three lines of chemotherapy left him with chronic pe

47 His visual acuity continued deteriorating.

48 His work on phagocytosis has continued to flourish, part

49 His(85) is a potential proton donor to reactive iron-oxo

50 His-143 and His-189 also increase the pKa of the pyridin

51 onto the active site containing the Ser-101-His-237-Asp-212 catalytic triad.

52 The most potent peptide 12f [His(2-biphenyl)-Trp-His(2-biphenyl)] displayed high in v

53 involves residues Asp-222, His-143, Thr-139, His-189, and structural waters, is located at the edge o

54 is network, which involves residues Asp-222, His-143, Thr-139, His-189, and structural waters, is loc

55 A 23mer His-based peptide derived from human fatty acyl-CoA redu

56 We also found that 3 His residues in the loop appear to temporarily coordinat

57 tability in vitro The effects of the Asp-302-His-305 salt bridge are thus complex and context-depende

58 ubstrate, and thus, a flexible loop (Glu-334-His-343) is essential in binding sucrose and beta(2-1)-l

59 e revealed a critical role for a conserved 4-His motif in forming the CCD iron center.

60 surface including the side chains of Arg 6, His 11 and Lys 32 as potentially important in the FS50 N

61 with a single zinc ion coordinated by His-6, His-8, His-179, and Glu-282.

62 bound by a Ser-93-fatty acid carboxyl-Thr-61-His-266 hydrogen bond network.

63 aspartate catalytic triad comprising Cys-73, His-162, and Asp-182.

64 used on OleTJE active site residues Phe(79), His(85), and Arg(245) to interrogate their roles in subs

65 single zinc ion coordinated by His-6, His-8, His-179, and Glu-282.

66 By attaching a His-tag to an enzyme, a dual-functional catalyst was cre

67 possess an N-terminal extension containing a His- and Asp/Glu-rich hypervariable region followed by a

68 tion and immobilization of SrtA containing a His-tag from the fermentation supernatant onto a nickel-

69 Both the patient and her father had a His-58(E7) --> Leu mutation in alpha1.

70 nated by two His ligands, one of which has a His-brace in which the His-1 terminal amine group also c

71 Structural analysis of Fpn reveals a His-Cys catalytic dyad that is characteristic of C11-fam

72 Our data collectively support that a His residue on the protein scaffold polarizes a water mo

73 ed to bind tetradentate siderophores using a His and a Tyr side chain to complete the Fe(III) coordin

74 Thermoplasma acidophilum, by introducing a (His)6-tag within a loop in the alpha subunit of the comp

75 sts, 18 [Ac-Arg-Arg-(pI)DPhe-Tic-NH2], 1 [Ac-His-Arg-(pI)DPhe-Tic-NH2], and 41 [Ac-Arg-Arg-(pI)DPhe-D

76 73 nM) than the melanocortin tetrapeptide Ac-His-DPhe-Arg-Trp-NH2.

77 s-19, indicating that the solvent-accessible His-27 facilitates proton conduction of the channel by i

78 An analogous His residue present in some CNG channels is an inhibitor

79 C has an active site composed of Cys-129 and His-269, and the surrounding active site cleft is hydrop

80 His-143 and His-189 also increase the pKa of the pyridine nitrogen b

81 ilent, but a double mutant with His(273) and His(274) exchanged for asparagines exhibits a much less

82 rogen-bonded salt bridge between Asp-302 and His-305 is particularly interesting as Asp-302 is the si

83 analyses revealed that residues His-333 and His-350 in the cytoplasmic C-terminal domain were requir

84 riant enzymes suggested roles for Glu-48 and His-164 in the catalytic mechanism.

85 Two active site residues, Asp-547 and His-466, were also examined and shown by site-directed m

86 ve that atypical residues of Hb, His(58) and His(89) of alphaHb, coordinate to the heme iron, which i

87 s by site-directed mutagenesis of Glu-87 and His-89.

88 Replacement of the residues His-936 and His-917 in the activation and catalytic loops, respectiv

89 iochemical studies indicated that Tyr 97 and His 138 are key residues for catalytic reaction and that

90 cluding human thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied, and the re

91 cytoplasmic extensions that contain Cys- and His-rich high affinity metal binding domains, which may

92 uting diaminopropionic acid (Dap), DDap, and His at the Asn position yielded potent MC4R ligands, whi

93 tructures complexed with imidazole, HOL, and His with NAD(+) provided in-depth insights into the enzy

94 ar pacing, left ventricular (LV) pacing, and His-bundle pacing.

95 ligated water for the hydration reaction and His-164 acts as a catalytic acid.

96 residues Asp-45 and Asp-49 in the second and His-153 and Asp-157 in the fifth transmembrane segments

97 n helix 1, which contains the N terminus and His-3, and has been associated with cognate metal bindin

98 the aromatic amino acids Phe, Tyr, Trp, and His within peptide medicinal chemistry are showcased her

99 anar (including aromatic (Phe, Trp, Tyr, and His)/amide (Asn and Gln)/Guanidine (Arg)) side-chains an

100 oncovalent formats efficiently elicited anti-His tag antibodies.

101 For example, basic residues (Arg, His, Lys) increase peptide retention when located closer

102 of the peptide hormone by binding to an Arg-His-Asn motif in IDA.

103 HyaA-R193L variant to disrupt a proposed Arg-His cation-pi interaction in the secondary coordination

104 ent the structure of the ZRANB3 HNH (His-Asn-His) endonuclease domain and provide a detailed analysis

105 The P domain contains an Asp-His-Ser catalytic triad that is, together with five resi

106 on to the membrane-bound Asp-His-His and Asp-His-His-associated (DHH/DHHA1) domain-containing phospho

107 of 5.3 in the boundary between cSer and Asp-His.

108 w that in addition to the membrane-bound Asp-His-His and Asp-His-His-associated (DHH/DHHA1) domain-co

109 overcomes the reliance on the conserved Asp-His hydrogen bonding interaction, leading to a catalytic

110 PRUNE is a member of the DHH (Asp-His-His) phosphoesterase protein superfamily of molecule

111 iers, glutamic acid, aspartyl-histidine (Asp-His), cycloserine (cSer), and arginine, which provided a

112 C3), a cellular Golgi apparatus-specific Asp-His-His-Cys (DHHC) zinc finger protein.

113 wn as DHHC3), a Golgi apparatus-specific Asp-His-His-Cys (DHHC) zinc finger protein; (ii) a GODZ domi

114 s valuable insights into the role of the Asp-His-Fe triad of heme peroxidases.

115 The Asp-His-His-Cys-Cys-rich domain-containing Protein S-Acyl Tr

116 n strongly stimulates autophosphorylation at His(183) in its C-terminal kinase domain.

117 ry time, Wenckebach cycle length, and atrial-His (AH) interval were measured before and after every r

118 e binds in a 6-coordinate complex with axial His and Cys ligands, the latter provided by a heme-regul

119 cytochrome c contains three hemes with axial His/Met ligation, whereas heme 3 exhibits the His/Cys co

120 pha5 helix and the pi-pi interaction between His(57) and Phe(189) In addition, the insertion mutant a

121 ral predictions identified a nitrate binding His (H362) in NPF6.6 but not in NPF6.4.

122 compositions, type of detection tag (biotin, His- or cMyc-tag), and spacer length.

123 ution with spectroscopic features of the bis-His species observed in the crystal structure.

124 ating that the photochemistry of metal-bound His is different from that of metal-free His.

125 olase, with a single zinc ion coordinated by His-6, His-8, His-179, and Glu-282.

126 ture, including loss of iron coordination by His-238 apparently from an altered conformation of the c

127 -linked oxygen and the active site catalytic His.

128 We also find that the pKa of the catalytic His substantially increases in CmpI, indicating that thi

129 revealed the position of the characteristic His-Cys catalytic dyad (His(99) and Cys(136)), and an As

130 omogeneity by anion exchange chromatography, His-Trap affinity chromatography and gel filtration.

131 sport affinity/selectivity using a conserved His residue.

132 the necessity for a second highly conserved His residue within the beta subunit.

133 nsensus C:G base pairs H-bond with conserved His or Arg residues in ZnF8, ZnF9, and ZnF11, and the co

134 ent polarity and length (i.e. Ala, Arg, Cys, His, Glu, and Leu) on transporter stability and function

135 des containing nucleophilic sidechains (Cys, His, and Lys) and selected proteins (bovine and human he

136 rfamily, and all possess a non-canonical Cys-His-Ser catalytic triad.

137 s build up H2N-Pro-Gly-Ala-CONHL and H2N-Cys-His-Asp-CONHL (where L = organic struts) amino acid sequ

138 l four sdAb-Fcs recognize the C-terminal Cys-His-rich domain of PCSK9.

139 L-cholesterol, and as tools to study the Cys-His-rich domain-dependent sorting the PCSK9-LDLR complex

140 tudies have shown that PCSK9 lacking its Cys/His-rich domain can still promote LDLR internalization,

141 GhCHR contains two DC1 and three PHD Cys/His-rich domains, suggesting that GhCHR encodes a zinc-f

142 t is the first time that histidine-cysteine (His-Cys) and histidine-lysine (His-Lys) in addition to h

143 were harnessed as a powerful means to direct His-tagged fluorophores to distinct locations within the

144 Mutation of the distal His(64) residue to alanine promotes rapid binding of H2S

145 oup, result in autophosphorylation of a DosS His residue, followed by phosphotransfer to an Asp resid

146 f the characteristic His-Cys catalytic dyad (His(99) and Cys(136)), and an Asp (Asp(134)) in the pote

147 factors of the PURE translation system, each His-tagged and in separate transcription cistrons.

148 e the ionization properties of lipid-exposed His, Lys, and Arg side chains in lipid bilayer membranes

149 trains have an unusual Phe 43 cavity-filling His 375 residue, which increases the propensity of Env t

150 esults suggest pKa values of less than 3 for His(12) and about 3-5 for His(14) in DOPC membranes.

151 of less than 3 for His(12) and about 3-5 for His(14) in DOPC membranes.

152 pability of detection was of 8.1 +/- 0.7 for His and 10 +/- 0.6 muM for Put.

153 standard deviation was of 3.5% and 6.7% for His and Put, respectively.

154 Here, we used rhDNase, which only forms His-His covalent dimers after light treatment to determi

155 und His is different from that of metal-free His.

156 The specific activity of TopA expressed from His-tagged fusion construct in the chromosome is inverse

157 earrangement, promoting a ligand switch from His-209 to His-212 and triggering heme release to HemO.

158 riants containing amino acids Ala, Asn, Gln, His, Ile, and Lys at positions equivalent to 782 and/or

159 oken down mainly into two fragments from Glp-His-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-NHEt (LHRHa) to Trp-Se

160 seven enantiomer pairs d/l-Ala, -Asp, -Glu, -His, -Leu, -Ser, -Val and the three achiral amino acids

161 ine-diglycolic acid-DPhe-Gln-Trp-Ala-Val-Gly-His-Leu-NHEt), showing excellent tumor localizing effica

162 ymethyl-piperidine-D-Phe-Gln-Trp-Ala-Val-Gly-His-Sta-Leu-NH2 ((68)Ga-RM2) is a synthetic bombesin rec

163 ymethyl-piperidine-d-Phe-Gln-Trp-Ala-Val-Gly-His-Sta-Leu-NH2 ((68)Ga-RM2) is a synthetic bombesin rec

164 e also observe that atypical residues of Hb, His(58) and His(89) of alphaHb, coordinate to the heme i

165 nd purified as an N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE mo

166 Zn very tightly only in the presence of high His and makes Zn bioavailable through His catabolism.

167 the simultaneous determination of histamine (His) and putrescine (Put).

168 Histidine (His) is proposed to be a key residue in cross-link gener

169 work-guided GWAS signal was for a histidine (His)-related trait in a region containing two genes: a c

170 m conserved tyrosine (Tyr-87) and histidine (His-38) residues within the active site.

171 His-Lys) in addition to histidine-histidine (His-His) cross-links were discovered in monoclonal antib

172 , and archaebacteria synthesise L-histidine (His) in a similar, multistep pathway that is absent in m

173 ct photochemical oxidation of the histidine (His) residue by (1)O2 is a major degradation pathway for

174 ZigA is encoded adjacent to the histidine (His) utilization (Hut) system.

175 resulting in an arginine (Arg) to histidine (His) substitution in its encoded protein, NIPA (Nuclear

176 BM2 contains a second titratable histidine, His-27, in the tetrameric transmembrane domain that form

177 we present the structure of the ZRANB3 HNH (His-Asn-His) endonuclease domain and provide a detailed

178 To understand how His-27 affects the proton conduction property of BM2, we

179 several amino acid substitutions (including His, Phe, Pro, Trp, and Tyr) support an enhanced viabili

180 o obtain 3D peptide crystals and incorporate His-tag-based cargoes and may have potential use in drug

181 We inserted His residues into position 12 or 14 of GWALP23 (replacin

182 ramework (MOF) based on the tripeptide Gly-l-His-Gly (GHG) for the enantioselective separation of met

183 and -replete conditions to mobilize a labile His-Zn pool.

184 es prepared from metallochelation liposomes, His-tagged antigen rOspA from Borrelia burgdorferi, and

185 ine-cysteine (His-Cys) and histidine-lysine (His-Lys) in addition to histidine-histidine (His-His) cr

186 a fully conserved histidine residue in MraY (His-289 in BsMraY), which has been experimentally confir

187 but not in ECs transfected with pcDNA3.1-myc-His-Phe(138)-CaM, the lysoPC-induced TRPC6-CaM dissociat

188 Cs transiently transfected with pcDNA3.1-myc-His-Phe(99)-CaM, but not in ECs transfected with pcDNA3.

189 Cs transiently transfected with pcDNA3.1-myc-His-Phe(99)-CaM.

190 ation states of Asp-219 and Glu-447, and not His, strongly determined the pH-dependent transport perm

191 nts led to the conclusion that the charge of His 212 in RasGRP1 alters signaling activity and plasma

192 tates/composition (different combinations of His protonation states or different metal-ligating ligan

193 successfully applied to the determination of His and Put in different food samples.

194 e the pH-dependent structure and dynamics of His-27.

195 ber of protein chains and thus the number of His ligands), and (4) ligand composition by evaluating t

196 , we demonstrate that the photoreactivity of His is determined by both solvent accessibility and stru

197 The pKa of His-305 is raised to 9.0, indicating the salt bridge sta

198 intriguing possibility that the presence of His 375 in the circulating strain where the RV144 trial

199 The response to protonation of His(14) is an increase in helix tilt, but GWALP23-H14 re

200 ogous expression and partial purification of His-tagged CesA5 from Physcomitrella patens Immunoblot a

201 increasing the proton dissociation rates of His-19.

202 the factors that influence the reactivity of His in proteins, especially the intrinsic factors are li

203 at influence the light-induced reactivity of His.

204 Conversely, the replacement of His 375 by a serine residue (H375S) within HIV-1 CRF01_A

205 phenylalanine indicated a functional role of His(163) in substrate binding.

206 n with WT OleTJE, indicating the key role of His(85) in this process.

207 To better understand the role of His-50, we analyzed the effect of DHA on aS-derived spec

208 Moreover, substitution of His-223, a conserved residue proposed to activate water

209 be responsible for the natural variation of His-related traits across the association panel.

210 an uncharacteristic five-coordinate base-off/His-off conformation, whereby the dimethylbenzimidazole

211 ble pacing options, with particular focus on His-bundle pacing.

212 of a set of 25 analogues featuring Ala(1) or His(1) and a variety of aromatic side chains at the aza-

213 ectively) but not conserved in E. coli OtsA (His, Leu, and Asp, respectively), providing a rationale

214 rnative techniques such as multisite pacing, His bundle pacing, and endocardial left ventricular paci

215 (Ptb33) and Tph (TN1) proteins using a Penta-His antibody, and both proteins were insoluble.

216 Incorporation of specific binding peptide (His Pro Gln: HPQ) gives M13 bacteriophage high selectivi

217 Permanent His-bundle pacing has emerged as the leading candidate f

218 thesized analogue, Ac-Arg-Ala-[d-Cys-Arg-Phe-His-Pen]-COOH (19), displayed subnanomolar affinity towa

219 anomolar agonist potency at the mMC4R, c[Pro-His-DPhe-Arg-Trp-Asn-Ala-Phe-DPro] and c[Pro-His-DPhe-Ar

220 His-DPhe-Arg-Trp-Asn-Ala-Phe-DPro] and c[Pro-His-DPhe-Arg-Trp-Dap-Ala-DPro], and may be further devel

221 Recombinant His-tagged trout CK12a (rCK12a) is not chemotactic in vi

222 of mda-7/IL-24 or treatment with recombinant His-MDA-7 protein resulted in downregulation of miR-221

223 hat is formed by three icosahedrally related His residues in the N termini of the C subunit at the qu

224 base pair H-bonds with an Asn that replaces His in ZnF10.

225 o-face pi-pi-stacking with the LRH-1 residue His-390, as well as by a novel polar interaction mediate

226 Remarkably, residue His-207 alone is sufficient to hydrolyze PGP, indicating

227 ns with the conserved Q-binding-site residue His-38 and that conformational changes between these bin

228 utagenesis we show that a histidine residue (His-347) downstream of S6 reduces inhibition by divalent

229 ficant rearrangements of side chain residues His(57), Phe(189), Phe(191), and Phe(336); it also distu

230 analysis suggests that pre-S1 loop residues His-402 and Tyr-403 play an important role in regulating

231 Further analyses revealed that residues His-333 and His-350 in the cytoplasmic C-terminal domain

232 Replacement of the residues His-936 and His-917 in the activation and catalytic loop

233 His residue with the side chain of a second His residue to create a T-shaped arrangement of nitrogen

234 ), encoding the constitutively active sensor His kinase that activates MSP signaling.

235 es suggest the involvement of invariant Ser, His, and Glu residues in metal coordination.

236 he acute hemodynamic effects of simultaneous His-bundle (HIS) and left ventricular (LV) pacing.

237 es in CmpI, indicating that this active site His is the source of the proton required in the reductio

238 Oligohistidine affinity tags (His-tags) are commonly fused to proteins to aid in their

239 dinative interaction of oligohistidine-tags (His-tags) with metal-organic framework nanoparticles (MO

240 inated antibodies directed to the C-terminal His tag located at the "bottom" of the spike.

241 -rich fragment of rSp0032 and the C-terminal His-rich fragment show unique transformations by either

242 amino group and side chain of the N-terminal His residue with the side chain of a second His residue

243 In these structures, the N-terminal His-tag and several neighboring residues play a highly u

244 The tetrapeptide His-DPhe-Arg-Trp or tripeptide DPhe-Arg-Trp replaced the

245 etrad protonates with higher pKa values than His-19, indicating that the solvent-accessible His-27 fa

246 Here, we provide evidence that His-50 is the main site of this covalent modification.

247 g binding and activity assays, we found that His 336 of UBA5, previously not reported to be part of t

248 Given that His is a semiessential amino acid and a potent metal che

249 plasma membrane recruitment, indicating that His 212 is a pH sensor that alters the balance between t

250 d for other amidohydrolases, suggesting that His-223 has a different role in LigY.

251 The His ammonia-lyase HutH binds Zn very tightly only in the

252 vation time relative to the QRS onset at the His bundle and successful ablation sites than the LV sum

253 is/Met ligation, whereas heme 3 exhibits the His/Cys coordination typical for TsdA active sites.

254 early activation was always recorded in the His bundle region, regardless of the location of the VA

255 talytically inactive Phe substitution in the His-Asp catalytic dyad of CurJ-DH to elucidate substrate

256 such as .OH at the non-heme iron site in the His-cluster region formed by the active site conversion.

257 This makes the His biosynthetic route a promising target for herbicides

258 icular summit in 4, and LVOT septum near the His bundle in 1.

259 summit, and rarely the LVOT septum near the His bundle.

260 rB by altering the natural regulation of the His operon.

261 munoprecipitation assay, substitution of the His or Cys heme ligands in Rev-erbbeta was accompanied b

262 alent dimer and rank the reactivities of the His residues in this protein.

263 tion, which accumulate in the absence of the His(64) ligand.

264 perform catalysis after introduction of the His-tag.

265 mational change is necessary to relocate the His-289 residue, such that the translocase reaction can

266 us envelope, (15)N NMR spectra show that the His-27 tetrad protonates with higher pKa values than His

267 ly acidic pH values in the endosome when the His(37) residues in the middle of the transmembrane doma

268 s, one of which has a His-brace in which the His-1 terminal amine group also coordinates to a copper.

269 These His-tags are generally assumed to have minimal impact on

270 The reactivities of these His residues were correlated with solvent accessibility-

271 This His is involved in UFM1 binding and if mutated perturbs

272 he Met-Gln-Trp sequence of the canonical Thr-His-Trp (THW) loop known to interact with the methyl-acc

273 f high His and makes Zn bioavailable through His catabolism.

274 el is regulated independently via titratable His.

275 t, promoting a ligand switch from His-209 to His-212 and triggering heme release to HemO.

276 Mutation of NPF6.4 Tyr-370 to His (Y370H) resulted in saturable high-affinity nitrate

277 t with our model, the mutation of Cys-431 to His in the THW loop of human PRMT9 shifts its product sp

278 The change of Asn at position 460 to His, which corresponds to the natural amino acid at the

279 and additionally a hydrogen bond (G1324S) to His(1322) across the beta2-beta3 hairpin in the GPIbalph

280 tidinaldehyde and from L-histidinaldehyde to His.

281 2 arylated histidines containing tripeptides His(2-Ar)-Trp-His(2-Ar) exhibit potent antifungal activi

282 G)), tRNA(Pro(UGG)), tRNA(Pro(CGG)) and tRNA(His(GUG)) for Um, and tRNA(Pro(GGG)) for Am. tRNA(Ser(UG

283 ses, and its members include eukaryotic tRNA(His) guanylyltransferase (Thg1), as well as Thg1-like pr

284 iTLP2 catalyzes a mitochondria-specific tRNA(His) maturation reaction, which is distinct from the tRN

285 on reaction, which is distinct from the tRNA(His) maturation reaction typically catalyzed by Thg1 enz

286 tidines containing tripeptides His(2-Ar)-Trp-His(2-Ar) exhibit potent antifungal activity against Cry

287 most potent peptide 12f [His(2-biphenyl)-Trp-His(2-biphenyl)] displayed high in vitro activity agains

288 hannel and to interact with His2 of the twin-His motif.

289 hat it contains a Cu atom coordinated by two His and one Cys in a trigonal plane, with an axial H2O a

290 center contains a Cu(II) coordinated by two His ligands, one of which has a His-brace in which the H

291 tion factor has been modified to include two His residues at designed i and i+4 positions of its N-te

292 Removing either the Cys residue or the two His residues lowers the Cu-peptide affinity, but site sp

293 Specifically, UFM1 His 70 resembles UBA5 His336 and enters a negatively cha

294 ntial functional role of the enzyme's unique His ligand.

295 selected for the external binding of various His-tagged synthetic peptides and recombinant or chemica

296 Under acidic conditions, when His(12) is protonated and charged, the GWALP23-H12 helix

297 mportant role in ligand recognition, whereas His-89 is essential for signal transduction to the kinas

298 Furthermore, the MtHDH complex with His and NAD(+) displays the cofactor molecule situated i

299 Phe(79) interacts with His(85), and Phe(79) mutants showed diminished affinity

300 remarkably silent, but a double mutant with His(273) and His(274) exchanged for asparagines exhibits