ライフサイエンスコーパス: His6 tag

1 ion vector that also introduced a C-terminal His6 tag.

2 que except that the MutL protein carried the His6 tag.

3 60% for the Nanobody lacking the C-terminal His6 tag.

4 terminus with either a FLAG-epitope tag or a His6-tag.

5 and detected in immunoblots using N-terminal His6-tagged AgrD.

6 In addition, GST-NR2B and His6-tagged alpha-actinin can bind simultaneously to mon

7 mbinant sorcin bound to cardiac and skeletal HIS6-tagged alpha1 C termini immobilized on Ni2+ resin.

8 s formed between 1-Cys Prx with a C-terminal His6 tag and GST pi upon incubation of the two proteins

9 that encode the PAS domain with and without His6 tags and expressed the PAS peptides in E. coli.

10 We purified polyhistidine (His6)-tagged and native Escherichia coli MiaA tRNA preny

11 ies assembled in vivo in the presence of the His6-tagged and untagged Rpb3.

12 Purified recombinant His6-tagged apobec-1 immobilized on beads depleted >90%

13 The results show that His6 tag-assisted chemical protein synthesis is a useful

14 engineered into an expression vector with a His6 tag at its amino terminus, and the protein was expr

15 ombinant alpha-galactosidase molecule with a His6 tag at its C-terminus was constructed by an overlap

16 The synthetic technique makes use of a His6 tag at the C terminus of the target polypeptide cha

17 1) was expressed using a vector containing a His6 tag at the N terminus.

18 a conventional single-His6 tag or two single-His6 tags at N- and C-termini.

19 1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharom

20 ypothesis, R. sphaeroides mutants expressing His6-tagged bc1 complexes with mutations at three aromat

21 yellow fluorescent protein (His6-eYFP), and His6-tagged collagenase G (His6-ColG), with sizes rangin

22 Of the his6-tagged constructs derived from NACK, NACK100 was in

23 Moreover, the double-His6 tag could serve simultaneously both for protein imm

24 Similarly, His6-tagged densin-180 or alpha-actinin fragments associ

25 latory protein was purified as an N-terminal His6-tagged derivative and characterised both with and w

26 In the present study, His6-tagged E1 alpha2 beta2 tetramers (171 kDa) denature

27 The presence of a His6 tag enables the isolation of peptide or protein pro

28 binding protein fusion form (MBP-NasR) and a His6-tagged form (His6-NasR).

29 nterfacial properties of a carboxyl-terminal His6-tagged form of apoB20.1 (apoB20.1H).

30 spectrometry confirmed production of intact, His6-tagged forms of CYP144A1-FLV and -TRV, with EPR dem

31 We have expressed His6-tagged fragments containing the sequence of the C-t

32 6E mutants of peripherin-2 were expressed as His6-tagged full-length proteins in Madin-Darby canine k

33 ted into the pRSET vector and expressed as a His6 tag fusion protein in Escherichia coli.

34 atable peptide sequence to the C-terminus of His6-tagged green fluorescent protein (GFP).

35 de, the M. xanthus gene was expressed with a His6 tag in E. coli cells.

36 ary of mutants whose combined members have a His6 tag inserted at likely every site in the original p

37 Treatment of an N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led t

38 on in conjunction with immobilized metal ion/His6-tag interactions to prepare highly purified recombi

39 The protocol described here uses recombinant His6-tagged KDPG aldolase for the synthesis of (S)-4-hyd

40 His6-tagged M. thermautotrophicus type II IPP isomerase

41 Gene-targeted mice that contain a His6-tagged mouse c-Myc cDNA, Myc(His), inserted head to

42 We demonstrated that the His6-tagged MuLV could be produced to high titers and co

43 HPLC assay analysis of each His6-tagged mutant indicated that F337I successfully pro

44 GFP), stable and tight binding of the double-His6 tag/Ni-NTA interaction was demonstrated by competit

45 A His6-tagged NR2B fragment associates with GST-densin or

46 Display of the His6 tag on the surface of Env endowed the vectors with

47 modified surfaces than a conventional single-His6 tag or two single-His6 tags at N- and C-termini.

48 In this work, the His6-tagged PduS cobalamin reductase from S. enterica wa

49 Expression of a human His6-tagged PKC-theta in Jurkat cells and purification b

50 d-biotin-tris-NTA (BTN) hybrid compound, any His6-tagged protein can be immobilized on the surface of

51 proteins in the same cell and isolating the His6-tagged protein followed by MutS immunoblotting afte

52 in Escherichia coli as catalytically active His6 tagged proteins, purified to homogeneity by affinit

53 RNA ligands were selected by binding to His6-tagged proteins and chromatography on nickel(II) ni

54 We also characterized His6-tagged proteins enriched on-plate by the Fe3O4@Al2O

55 BIAcore) was 10 times slower than for single-His6-tagged proteins.

56 he pET15b constructs produced NH2-terminally His6-tagged proteins.

57 The purified hexahistidine (His6)-tagged Pseudomonas aeruginosa RhlI protein was sub

58 formation by wild-type and mutant XRCC3 with His6-tagged Rad51C upon co-expression in bacteria, nicke

59 n investigated by Raman spectroscopy using a His6-tagged recombinant protein that self-assembles in v

60 These proteins have been expressed as Met-His6-tagged recombinant proteins and purified by metal c

61 Overexpression of GT78 with a His6-tag resulted in a 120-fold increase in GalT-activit

62 The His6-tagged Sec7 domain from ySec7p (rySec7d) synthesize

63 Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahistidines separa

64 for the assembly of growth factors employing His6-tagged single-domain antibodies (VHH).

65 ach can be used to determine quickly whether His6-tagged species are present in a sample.

66 We capture His6-tagged TFs using nickel-nitrilotriacetic acid (Ni-N

67 attached to the carboxy end of an N-terminal His6 tag that binds to nickel (Ni2+).

68 Therefore, addition of the His6 tag to the C-terminus of RB69 RegA does not dramati

69 xpressed in Escherichia coli with N-terminal His6 tags to facilitate purification.

70 quipped lipid to bind the proteins via their His6-tags to the lipid membrane were used to bind two di

71 His6-tagged Tsc10p was expressed in Escherichia coli and

72 ptors and an NTA moiety to interact with the His6-tag upon cocomplexation of Ni(2+) ions.

73 To test this hypothesis, a six-histidine (His6)-tagged version of MM1854 was produced.

74 ecifically recognizing Rpl17 or Rpl39, and a His6-tagged version of Rpl4, we established that all thr

75 A His6-tagged version of the SCO1135 protein product was s

76 A recombinant His6-tagged version of this protein was subsequently pro

77 A His6 tag was cloned at the N terminus, along with R403Q,

78 Under denaturing conditions when the His6 tag was used, 84% of samples were purified.

79 gene, encoding a protein with an N-terminal His6 tag, was expressed in Escherichia coli.

80 NBD1-R (amino acids 404-830, in fusion with His6 tag) were expressed as single proteins in Escherich

81 9-kDa chimeric proteins, having a C-terminal His6 tag, were secreted by Saccharomyces cerevisiae, usi

82 e-steady-state studies were performed on the His6-tagged wild-type (WT) enzyme, several active site m

83 Dimerization was assayed by expressing His6-tagged wild-type and non-tagged deletion mutant pro

84 essed in Escherichia coli, and the resulting His6-tagged WreQ protein was purified.