ライフサイエンスコーパス: Holliday junction

1 DNA structure in these two processes is the Holliday junction.

2 ecules connected to each other by a flexible Holliday junction.

3 rently due to cleavage of the regressed fork Holliday junction.

4 ubstrates and include the D-loop and partial Holliday junction.

5 ite-specific double-stranded end (DSE) and a Holliday junction.

6 romoting second end capture to form a double Holliday junction.

7 is relaxed can RuvAB bind to a RecG-extruded Holliday junction.

8 nforced when the protein was shown to unwind Holliday junctions.

9 involve the coordinated cleavage of classic Holliday junctions.

10 stall at UV lesions and regress, generating Holliday junctions.

11 omplex) to promote the dissolution of double Holliday junctions.

12 ctivated in meiosis II to resolve persistent Holliday junctions.

13 imic B-DNA, to predict the properties of DNA Holliday junctions.

14 strand exchanges through branch migration of Holliday junctions.

15 'late' roles, such as dissolution of double Holliday junctions.

16 ture-specific endonucleases that cleave most Holliday junctions.

17 ly branched DNA intermediates containing few Holliday junctions.

18 to form four-stranded structures resembling Holliday junctions.

19 NA structures that include forks, flaps, and Holliday junctions.

20 cts the symmetric cleavage and resolution of Holliday junctions.

21 anched four-stranded DNA structures known as Holliday junctions.

22 ry structures at the replication fork and at Holliday junctions.

23 owed that Rad54 promotes branch migration of Holliday junctions.

24 ectly convert stalled replication forks into Holliday junctions.

25 r modification state is negligibly active on Holliday junctions.

26 for its unique ability to decatenate double-Holliday junctions.

27 replication forks, and dissolution of double Holliday junctions.

28 erence, suggest a conserved binding mode for Holliday junctions.

29 at are organized and dictated by a series of Holliday junctions.

30 h affinity and shows a marked preference for Holliday junctions.

31 ppa joints, synthetic replication forks, and Holliday junctions.

32 exchange of DNA between chromatids, forming Holliday junctions.

33 ts limited endonuclease activity with intact Holliday junctions.

34 bundle also inhibits the cleavage of intact Holliday junctions.

35 m duplex, such as fork structures and nicked Holliday junctions.

36 nd exchange and promotes branch migration of Holliday junctions.

37 trates, while the latter exclusively targets Holliday junctions.

38 roximately 1 min(-1)) and include the nicked Holliday junction, 3'-flapped and replication fork-like

39 omplex designs including linear, bifurcated, Holliday junction, 8-arm star and dendrimers involving u

40 branched structures other than the canonical Holliday junction, a feature advantageous for viral DNA

41 own to catalyze branch migration (BM) of the Holliday junction, a key intermediate in DNA repair and

42 nzymes include the ability to resolve double Holliday junctions, a process thought to lead to the sup

43 sW and UvsW-K141R are capable of stabilizing Holliday junctions against spontaneous branch migration

44 es is determined by the effective density of Holliday junctions along a particular stress direction.

45 lly, Red1 preferentially associated with the Holliday junction and 3-way junction rather than with si

46 tS homologs, hMSH4-hMSH5, bind uniquely to a Holliday Junction and its developmental progenitor.

47 ing protein exhibiting high affinity for the Holliday junction and promoting DNA bridging, condensati

48 tion and six were found to confer defects in Holliday junction and replication fork cleavage in vivo.

49 the Mut S homolog 4 (Msh4) and Msh5 bind to Holliday junctions and are required for homologous recom

50 induce ATP-dependent unwinding of synthetic Holliday junctions and ATP-stimulated annealing of uncon

51 In bacteria, the RuvAB complex binds Holliday junctions and catalyses ATP-dependent branch mi

52 activation does not induce a preference for Holliday junctions and does not induce multimerization o

53 EM visualization of BCDX2 and CX3 binding to Holliday junctions and forked DNAs and suggests the comp

54 Aberrant JMs contain high levels of single Holliday junctions and include intersister JMs, multichr

55 is capable of binding three-way and four-way Holliday junctions and model replication forks that lack

56 show SLX1-dependent nuclease activity toward Holliday junctions and MUS81-dependent activity toward o

57 nd human Rad54 (hRad54) specifically bind to Holliday junctions and promote branch migration.

58 le nucleases in order to effectively resolve Holliday junctions and repair interstrand crosslinks (IC

59 NCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro.

60 e active site is responsible for cleavage on Holliday junctions and splayed duplexes.

61 s, which resulted in processing of synthetic Holliday junctions and stabilization of model replicatio

62 p3 inhibited the Hel112 helicase activity on Holliday junctions and stimulated formation and stabiliz

63 tion on two major activities of RAD54, BM of Holliday junctions and stimulation of DNA pairing.

64 sion intermediates are converted into double Holliday junctions and then resolved into crossovers tha

65 eaves 3'-flaps, replication forks and nicked Holliday junctions, and exhibits limited endonuclease ac

66 ding of homologous pairing, and migration of Holliday junctions, and they suggest that various functi

67 Holliday junctions are critical intermediates in DNA rec

68 Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replicatio

69 Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombi

70 The single-crystal structure of a DNA Holliday junction assembled from four unique sequences s

71 Holliday junctions assembled from oligonucleotides with

72 In both conditions, Holliday junctions at DNA break hotspots form more frequ

73 ependent, X-shaped DNA structures containing Holliday junctions between sister chromatids.

74 Our kinetic studies of Holliday junction branch migration catalysed by a ring-s

75 etic evidence, has been proposed to act as a Holliday junction branch migration enzyme.

76 sses such as replication fork regression and Holliday junction branch migration.

77 nt protein, UvsW-K141R, is unable to promote Holliday junction branch migration.

78 n of DSBs and then defines the boundaries of Holliday junction branch migration.

79 as at most a minor role in resolution of the Holliday junction but acts as an essential backup to MUS

80 MUS81-EME1, SLX1-SLX4 and GEN1 also process Holliday junctions but, in contrast to the BTR complex,

81 Recombinant GEN1 and Yen1 resolve Holliday junctions by the introduction of symmetrically

82 RecA-generated Holliday junctions can be detected by RuvC cleavage, alt

83 In somatic cells, Holliday junctions can be formed between sister chromati

84 involves replication fork regression into a Holliday junction ('chicken foot structure'), DNA synthe

85 ructural similarity is found to the archaeal Holliday junction cleavage enzymes, strongly suggesting

86 Holliday junction cleavage requires the coordination of

87 clease complex [gene product (gp) 46/47] and Holliday junction-cleaving enzyme (EndoVII), respectivel

88 e and RuvC, and a model of the CPV resolvase.Holliday junction complex provides insights into the con

89 wever, structural superposition on bacterial Holliday junction complexes and similarities in the C-te

90 the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are r

91 Using a novel Holliday junction-containing substrate, we also demonstr

92 nerated replication intermediates, including Holliday junction-containing X-shaped intermediates and

93 The active form of the enzyme on the Holliday junction contains a single RuvA tetramer.

94 Our results indicate that Holliday junctions control mechanical behaviors of DNA n

95 of a sliding clamp that dissociated from the Holliday Junction crossover region embracing two duplex

96 molecules, a protein-DNA complex and dynamic Holliday junctions demonstrate the potential of switchab

97 pha) is an essential component of the double Holliday junction (dHJ) dissolvasome complex in metazoan

98 olliday junction (HJ) or dissolve the double Holliday junction (dHJ) structure to yield non-crossover

99 This complex can resolve the double Holliday junction (dHJ), a DNA intermediate generated du

100 The double Holliday junction (DHJ), presupposed to be the central i

101 formation via the dissolution of the double Holliday Junction (dHJ).

102 -break repair (DSBR) by disassembling double-Holliday junctions (dHJs) or by preventing their formati

103 a homology search, ultimately forming double Holliday junctions (dHJs) that primarily include both ho

104 tion of crossover-specific JMs called double Holliday junctions (dHJs).

105 , branch migration, and resolution of double Holliday junctions (dHJs).

106 and MutL, may utilize its ability to unwind Holliday junctions directly in the prevention of homeolo

107 rement for the BLM ATPase activity in double Holliday junction dissolution and DNA unwinding by const

108 two stages: 5' DNA end resection and double Holliday junction dissolution.

109 nalyse human cells compromised for the known Holliday junction dissolution/resolution pathways.

110 in the disease biochemically functions as a Holliday junction dissolvase and human cells lacking fun

111 domains to the four "core-type" sites of the Holliday junction DNA and via its amino-terminal domains

112 d the "dissolvasome," which separates double-Holliday junction DNA structures that can arise during D

113 and breaks and also in the resolution of the Holliday junction during homologous recombination.

114 in independent pathways that branch migrate Holliday junctions during recombinational DNA repair.

115 le, we studied the effect of the sequence on Holliday junction dynamics and branch migration process.

116 een applied to new experimental FRET data of Holliday Junction dynamics, extracting the expected two

117 e that UvsW promotes the branch migration of Holliday junctions efficiently through more than 1000 bp

118 Holliday junctions enable modification with multiple flu

119 can dissociate DNA structures, including the Holliday junction, expected to arise during homologous r

120 Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form.

121 trate that WRN catalyzes fork regression and Holliday junction formation.

122 break repair pathway and that resolution of Holliday junctions formed as part of the latter pathway

123 pair during replication and can also resolve Holliday junctions formed during homologous recombinatio

124 wed the reaction intermediate transitions as Holliday junctions formed through catalysis by XerD, iso

125 is more efficient at generating the initial Holliday junction from the stalled fork.

126 nd lagging strands, whereas RuvC cleaves the Holliday junction generated by RecG-catalyzed fork regre

127 -sites one and four (H14 mode) and recognize Holliday junction geometry-specifically.

128 ediate in homologous recombination, and such Holliday junctions have since been identified as a centr

129 can be produced, including migratable single Holliday junctions, hemicatenanes and a quadruple Hollid

130 cG helicase of Escherichia coli unwinds both Holliday junction (HJ) and replication fork DNA substrat

131 interacts with DNA, using an engineered DNA Holliday junction (HJ) as a protein-DNA interaction repo

132 forces on the nanometer scale motion of the Holliday junction (HJ) at 100-hertz bandwidth.

133 The assembly is a 240-bp Holliday junction (HJ) bound specifically by 11 protein

134 role in DNA unwinding, strand annealing, and Holliday junction (HJ) branch migration.

135 rmediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent t

136 ologous recombination, from end resection to Holliday junction (HJ) dissolution.

137 nd this domain preferentially binds fork and Holliday junction (HJ) DNA in vitro and is required for

138 ed in the mus81Delta mutant contain a single Holliday junction (HJ) explaining why STR is unable to p

139 in, FtsK, which activates the formation of a Holliday Junction (HJ) intermediate by XerD catalysis th

140 ter which a pair of strand exchanges forms a Holliday junction (HJ) intermediate; HJ isomerization th

141 y Rec12 (Spo11 homolog) and generates single Holliday junction (HJ) intermediates, which are resolved

142 hase, by polo kinase-triggered resolution of Holliday junction (HJ) intermediates.

143 recombination intermediates (Rec-Xs) but not Holliday junction (HJ) intermediates.

144 The Holliday junction (HJ) is a central intermediate in homo

145 The Holliday junction (HJ) is a central intermediate of homo

146 The Holliday junction (HJ) is a central intermediate of vari

147 Holliday junction (HJ) is a hallmark intermediate in DNA

148 BLM-Topo IIIalpha pair to branch migrate the Holliday junction (HJ) or dissolve the double Holliday j

149 Based on the marked reduction in Holliday junction (HJ) resolution activity in Rad51c-nul

150 Holliday junction (HJ) resolution is essential for chrom

151 Holliday junction (HJ) resolution is required for segreg

152 nhibits telomere t-loop deletion by blocking Holliday junction (HJ) resolvase cleavage activity, but

153 Holliday junction (HJ) resolvases are necessary for the

154 Holliday junction (HJ) resolvases are structure-specific

155 the ruvC and yqgF genes that encode putative Holliday junction (HJ) resolvases.

156 Holliday junction (HJ) resolving enzyme RecU is involved

157 The Holliday junction (HJ), a cross-shaped structure that ph

158 The Holliday junction (HJ), or four-way junction, is a centr

159 f four-way DNA structures referred to as the Holliday junction (HJ).

160 Holliday junctions (HJs) are four-way DNA intermediates

161 Holliday junctions (HJs) are key DNA intermediates in ho

162 Holliday junctions (HJs) are physical links between homo

163 Holliday junctions (HJs) are X-shaped DNA structures tha

164 ought to arise from the resolution of double Holliday junctions (HJs) by an HJ resolvase.

165 on of recombination intermediates containing Holliday junctions (HJs) is critical for genome maintena

166 ences between the exchange sites to generate Holliday junctions (HJs) that contain mismatched bases.

167 generalizable method of using engineered DNA Holliday junctions (HJs) that contain specific protein-r

168 Holliday junctions (HJs) that physically link sister chr

169 symmetrical cleavage of static and migrating Holliday junctions (HJs), identifying SLX1-SLX4 as a HJ

170 are endonucleases with the ability to cleave Holliday junctions (HJs), which are proposed intermediat

171 hromosomes are covalently linked by four-way Holliday junctions (HJs).

172 These fluorescent Holliday junctions improve fluorescence yields for both

173 gulate recombination by disassembling double Holliday junctions in a process called dissolution.

174 Rad52-promoted annealing in the formation of Holliday junctions in DSB repair.

175 ast to their free-solution conformation, the Holliday junctions in the DNA origami structures adopt a

176 results from stabilization of peptide-bound Holliday junctions in the square planar conformation.

177 Mus81-Mms4 nuclease activity on 3' flaps and Holliday junctions in vitro but that activation does not

178 t harbors two Holliday junctions (the double Holliday junction) in a non-crossover manner.

179 Hence, Holliday junction incision activity in vitro cannot be a

180 tner site) not only allowed formation of the Holliday junction intermediate but also increased the ra

181 tate the formation and later resolution of a Holliday junction intermediate during recombination.

182 in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolu

183 of complexes between the recombinase and the Holliday junction intermediate of several highly diverge

184 NBU1 integration proceeds via formation of a Holliday junction intermediate that is formed by exchang

185 f stalled replication forks by unwinding the Holliday junction intermediate to allow bypass of the bl

186 and rejoin single strands in pairs to form a Holliday junction intermediate.

187 diated recombination block the resolution of Holliday junction intermediates in vitro and thereby inh

188 ifsy-1, Gifsy-2, Fels-1, Fels-2) and to trap Holliday junction intermediates of phage lambda site-spe

189 ctivates XerD catalytic activity to generate Holliday junction intermediates that can then be resolve

190 plication fork reversal via the formation of Holliday junction intermediates, suggesting that RuvAB-c

191 tion in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during m

192 A templates containing replication forks and Holliday junctions, intermediates observed during DNA re

193 A templates containing replication forks and Holliday junctions, intermediates observed during DNA re

194 o VII) was the first enzyme shown to resolve Holliday junctions into duplex DNAs by introducing symme

195 The Holliday junction is a central intermediate in various g

196 The Holliday junction is known to have an antiparallel orien

197 Significantly, a substrate containing a Holliday junction is unwound most efficiently.

198 omplex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic

199 Processing of Holliday junctions is essential in recombination.

200 The four-way junction (4WJ), or Holliday junction, is a dynamic DNA structure involved i

201 proteins may cooperate in the processing of Holliday junction-like intermediates during homologous r

202 Mus81-Eme1 endonuclease activity on various Holliday junction-like intermediates.

203 randed DNA junctions (Holliday junctions) or Holliday junction-like structures containing three of th

204 can ensure coordinate, bilateral cleavage of Holliday junction-like structures.

205 ted to each of its neighbors via two or more Holliday-junction-like reciprocal exchanges, such that e

206 rand annealing, rather than through a double Holliday Junction mechanism.

207 ermediates such as D-loops and static X-DNA (Holliday junction mimic) to ssDNA products is enhanced b

208 synthetic oligonucleotides via a four-armed Holliday junction motif.

209 rred regressed fork substrate for RuvAB is a Holliday junction, not a forked DNA.

210 the complex transits the open ends of model Holliday Junction oligonucleotides.

211 m where a single-stranded DNA nanostructure (Holliday junction or paranemic cross-over DNA) is insert

212 to be active on four-stranded DNA junctions (Holliday junctions) or Holliday junction-like structures

213 such as displacement loops (D-loops), nicked Holliday junctions, or 3' flaps but not intact Holliday

214 ting a previously unappreciated affinity for Holliday junctions over other substrates.

215 Holliday junctions play a central role in genetic recomb

216 hat normally replicating human cells require Holliday junction processing activities to prevent siste

217 We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 pr

218 ase by the CENP-A-specific histone chaperone Holliday junction recognition protein (HJURP) coupled wi

219 serve that the centromere-specific chaperone Holliday Junction Recognition Protein (HJURP) stabilizes

220 otein A), deposited by its chaperone, HJURP (Holliday junction recognition protein).

221 t ADP-->ATP exchange is uniquely provoked by Holliday Junction recognition.

222 s Escherichia coli host chromosome through a Holliday junction recombination intermediate.

223 ted chemical cross-linking of Int in trapped Holliday junction recombination intermediates and recomb

224 omic structure of a resolvase complex with a Holliday junction remained elusive.

225 fission yeast mus81Delta strains results in Holliday junction resolution and crossover formation dur

226 ng yeast polo-like kinase, is sufficient for Holliday junction resolution and exit from pachytene.

227 RAD51C) participates in branch migration and Holliday junction resolution and thus is important for p

228 human GEN1 protein and show that it promotes Holliday junction resolution by a mechanism that is anal

229 papers suggest that MUS312 and BTBD12 direct Holliday junction resolution by at least two distinct en

230 e XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that i

231 yces cerevisiae and human cells that promote Holliday junction resolution, in a manner analogous to t

232 inates the SLX1 and MUS81-EME1 nucleases for Holliday junction resolution, in a reaction stimulated b

233 ologous strand exchange and RuvABC-catalyzed Holliday junction resolution.

234 gene fusion library for nucleases capable of Holliday junction resolution.

235 s, topoisomerase I (TOP1) inhibitors, and in Holliday junction resolution.

236 is HP0334 (dprB), annotated to be a putative Holliday junction resolvase (HJR).

237 Mlh3 may not act like a canonical, RuvC-like Holliday junction resolvase and support a novel model in

238 re resolved prior to cell division, the Yen1 Holliday junction resolvase is activated at anaphase.

239 The virus-encoded Holliday junction resolvase is required to process conca

240 logous to that shown by the Escherichia coli Holliday junction resolvase RuvC.

241 atemer substrate by employing a portal-bound Holliday junction resolvase that trims and releases thes

242 They also need RecA recombinase and a Holliday junction resolvase to survive rapid growth, but

243 dc14 and, using this insight, identified the Holliday junction resolvase Yen1 as a DNA repair target

244 The human Holliday junction resolvase, GEN1, and its yeast ortholo

245 Another nuclease, the Mus81-Eme1 Holliday junction resolvase, is required to generate cro

246 leases and has been recently identified as a Holliday junction resolvase, results in increased sensit

247 Therefore, the yeast CCE1 gene, encoding a Holliday junction resolvase, was introduced into cells c

248 In addition, our study revealed a Holliday junction resolvase-like activity in the liver t

249 contains two domains, a third OB fold and a Holliday junction resolvase-like domain.

250 the gammaH2AX-binding protein Brc1 and Mus81 Holliday junction resolvase.

251 d-break repair and SOS induction, and RuvABC Holliday-junction resolvase.

252 Viral and bacterial Holliday junction resolvases differ in specificity with

253 ll shape and surface charge potential of the Holliday junction resolvases endo VII, RuvC, Ydc2, Hjc a

254 tructure provides insights into how archaeal Holliday junction resolvases evolved to incise 5' flap s

255 Several Holliday junction resolvases have since been characteriz

256 The eukaryotic Holliday junction resolvases represent a new subclass of

257 mosomal compaction, topoisomerase action and Holliday junction resolvases, ensure that all SCIs are r

258 ly of its nuclear role as a component of the Holliday junction-resolving BLM-Top3alpha-RMI1-RMI2 (BTR

259 cise structurally distinct bubbles, ends, or Holliday junctions, respectively.

260 eins responsible for branch migration of the Holliday junction(s) and reversal of stalled replication

261 Quantification of Holliday junctions showed that while recombination at ce

262 junction, and coupling required the complete Holliday junction structure.

263 n the ability to unwind replication fork and Holliday junction structures but has no effect on substr

264 ults indicate that RecG and RuvB compete for Holliday junction structures in recombinational repair,

265 oreover, the DprB protein binds to synthetic Holliday junction structures rather than double-stranded

266 catalyzes unwinding of replication fork and Holliday junction structures.

267 lyzes coordinate bilateral cleavage of model Holliday-junction structures.

268 wed that cleavage of a fluorescently labeled Holliday junction substrate did not yield an appreciable

269 nd reduced unwinding activity on a synthetic Holliday junction substrate relative to full-length GcRe

270 ingle turnover kinetic analysis revealed the Holliday junction substrate was cleaved 90-fold faster t

271 near DNA, and had the highest affinity for a Holliday junction substrate, illustrating a previously u

272 r creating a novel DNA substrate, the double Holliday junction substrate.

273 day junctions, hemicatenanes and a quadruple Holliday junction substrate.

274 eveal that recognition of 3'-flap and nicked Holliday junction substrates by Mus81-Mms4 involves indu

275 UvrD also catalyzes the robust unwinding of Holliday junction substrates.

276 and actively resolves telomeric D-loops and Holliday junction substrates.

277 mi with rotational symmetric arrangements of Holliday junctions, such as those in DNA nanotubes.

278 This substrate contains two Holliday junctions that are mobile, topologically constr

279 Strand invasion events mature into double Holliday junctions that can be resolved as crossovers.

280 y question is whether Mus81-Mms4 can process Holliday junctions that contain four uninterrupted stran

281 Holliday junctions that contain identical sequences betw

282 t a role for UvsW in the branch migration of Holliday junctions that form during T4 recombination, re

283 tly resolve a DNA substrate that harbors two Holliday junctions (the double Holliday junction) in a n

284 ase can dissolve DNA conjoined with a double Holliday junction, thus reducing DNA linkage.

285 results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic

286 ation involves the nucleolytic resolution of Holliday junctions to generate crossovers.

287 phores installed in close proximity, we used Holliday junctions to label proteins site-specifically.

288 A variety of processes act upon Holliday junctions to remove them from DNA, in events th

289 formation and preferential migration of the Holliday junctions to the boundaries of sequence homolog

290 We used nicked substrates and a Holliday junction trapping peptide to show that NBU1 int

291 Here we show that the Holliday junction unwinding activity of BLM is greatly e

292 ubstituted by Escherichia coli Top3, and the Holliday junction unwinding activity of BLM-related heli

293 In vitro, Rad54 promotes branch migration of Holliday junctions, whereas the Mus81-Eme1 complex resol

294 The inverted repeats refold to generate Holliday junctions, which are cleaved by the virus-encod

295 ibed to the collective actions of individual Holliday junctions, which are only possible in DNA origa

296 stabilizes invasion intermediates and double Holliday junctions, which are resolved into crossovers i

297 We designed a Holliday junction with three of its four arms modified w

298 Crucially, these two variants cleaved Holliday junctions with enhanced specificity and symmetr

299 lliday junctions, or 3' flaps but not intact Holliday junctions with four uninterrupted strands.

300 sired nanostructure by DNA staples that form Holliday junctions with the template.