ライフサイエンスコーパス: Hyp

1 r proteins to form (2S,4R)-4-hydroxyproline (Hyp).

2 (Pro) and Y is (2S,4R)-4-hydroxyproline (4R-Hyp).

3 tides additionally contain 4-hydroxyproline (Hyp).

4 a Pro residue to produce 2-hydroxyproline (2-Hyp).

5 hesis to incorporate Fmoc-hydroxyproline (4R-Hyp).

6 on and quantification of L-4-Hydroxyproline (Hyp).

7 A did not affect RPP but normalized dP/dt in HYP.

8 al reversibility of increased anaplerosis in HYP.

9 ates, and forms succinate and CO2 as well as Hyp.

10 n, where Xaa usually is Pro and Yaa often is Hyp.

11 ontext of cis-trans isomerization of Pro and Hyp.

12 nce of a hydration network in the absence of Hyp.

13 to use free l-proline as a precursor to form Hyp.

14 as source for the hydroxyl group oxygen of 2-Hyp.

15 in the order Pro < Hyp < [alpha-(1,4)GlcNAc]Hyp.

16 the safety and immunogenicity of daclizumab HYP.

17 nicity of extended treatment with daclizumab HYP.

18 HyP-1 exhibits selectivity for hypoxic activation in vit

19 HyP-1 features an N-oxide trigger that is reduced in the

20 HyP-1 is also compatible with NIR fluorescence imaging,

21 Reduction of HyP-1 produces a spectrally distinct product, facilitati

22 ese limitations, we present Hypoxia Probe 1 (HyP-1), a hypoxia-responsive agent for photoacoustic ima

23 We show that Hyp(10) disrupts only a small region of the alpha-helix

24 Replacement of Hyp(10) with N(8)Q results in a Mg(2+)-complexed end-to-

25 However, replacement of Hyp(10) with Pro(10) allowed the resulting peptide to re

26 (Gly-Pro-4(R)Hyp)(10) folds approximately 2000 times faster than (Gly

27 ted that the polypeptide Ac-(Gly-4(R)Hyp-4(R)Hyp)(10)-NH(2) forms a triple helix that is more stable

28 lix that is more stable than Ac-(Gly-Pro-4(R)Hyp)(10)-NH(2).

29 Hyp-[(14)C]Gal monosaccharide and Hyp-[(14)C]Gal disaccharide were identified in the base

30 Hyp-[(14)C]Gal monosaccharide and Hyp-[(14)C]Gal disacch

31 an SHAM but was lowered with PDH activation: HYP=1419+/-220 nmol/g dry weight; HYP+DCA=343+/-56 nmol/

32 Both Hyp(2) and betaPro(2) substitution decreased receptor af

33 eptide H-(Gly-Pro-4(R)Hyp)3-(Gly-3(S)Hyp-4(R)Hyp)2-(Gly-Pro-4(R)Hyp)4-OH to 1.80 A resolution.

34 , both isolated from interferon alpha2b-(Ser-Hyp)(20), and a 14-residue Hyp-AG isolated from (Ala-Hyp

35 rm (Gly-Pro-Hyp)3-Gly-X-Y-Gly-X'-Y'-(Gly-Pro-Hyp)3 extends the study to adjacent tripeptide sequences

36 structure of the polypeptide H-(Gly-Pro-4(R)Hyp)3-(Gly-3(S)Hyp-4(R)Hyp)2-(Gly-Pro-4(R)Hyp)4-OH to 1.

37 peptide containing the isostere, Ac-(Gly-Pro-Hyp)3-Gly-psi[(E)CH C]-Pro-Hyp-(Gly-Pro-Hyp)4-Gly-Gly-Ty

38 of host-guest peptides of the form (Gly-Pro-Hyp)3-Gly-X-Y-Gly-X'-Y'-(Gly-Pro-Hyp)3 extends the study

39 -Pro-Hyp)3-Gly-psi[(E)CH C]-Pro-Hyp-(Gly-Pro-Hyp)4-Gly-Gly-Tyr-NH2, had a Tm value of 28.3 degrees C.

40 The fTHP [sequence: Gly-mep-Flp-(Gly-Pro-Hyp)4-Gly-Lys(Mca)-Thr-Gly-Pro-Leu-Gly-Pro-Pro-Gly-Lys(D

41 o-Leu-Gly-Pro-Pro-Gly-Lys(Dnp)- Ser-(Gly-Pro-Hyp)4-NH2] had a melting temperature (Tm) of 36.2 degree

42 (R)Hyp)3-(Gly-3(S)Hyp-4(R)Hyp)2-(Gly-Pro-4(R)Hyp)4-OH to 1.80 A resolution.

43 emonstrated that the polypeptide Ac-(Gly-4(R)Hyp-4(R)Hyp)(10)-NH(2) forms a triple helix that is more

44 port crystal the structure of the H-(Gly-4(R)Hyp-4(R)Hyp)(9)-OH peptide at 1.55 A resolution.

45 Indeed, the unfolded state of the H-(Gly-4(R)Hyp-4(R)Hyp)(9)-OH peptide seems to be stabilized by a p

46 r-mediated bridge observed in the H-(Gly-4(R)Hyp-4(R)Hyp)(9)-OH structure, as well as a higher amount

47 he polypeptide H-(Gly-Pro-4(R)Hyp)3-(Gly-3(S)Hyp-4(R)Hyp)2-(Gly-Pro-4(R)Hyp)4-OH to 1.80 A resolution

48 , and a 14-residue Hyp-AG isolated from (Ala-Hyp)(51)-green fluorescent protein.

49 -fold in the main body hypodermal syncytium, hyp 7.

50 zed and incorporated into a host Ac-(Gly-Pro-Hyp)8-Gly-Gly-Tyr-NH2 peptide to investigate the effect

51 f the 3634 genes were annotated as conserved HyP (9.5%) along with 887 HyP genes (24.4%).

52 TAG content in HYP (9.7+/-0.7 micromol/g dry weight) was lower than SHA

53 stal the structure of the H-(Gly-4(R)Hyp-4(R)Hyp)(9)-OH peptide at 1.55 A resolution.

54 the unfolded state of the H-(Gly-4(R)Hyp-4(R)Hyp)(9)-OH peptide seems to be stabilized by a preformed

55 ed bridge observed in the H-(Gly-4(R)Hyp-4(R)Hyp)(9)-OH structure, as well as a higher amount of tran

56 brate collagen has (2S,4R)-4-hydroxyproline (Hyp), a C(gamma)-exo-puckered Pro derivative, in the Xaa

57 ing multiple sclerosis to compare daclizumab HYP, administered subcutaneously at a dose of 150 mg eve

58 ssed in tobacco cells; that is, a 22-residue Hyp-AG and a 20-residue Hyp-AG, both isolated from inter

59 eron alpha2b-(Ser-Hyp)(20), and a 14-residue Hyp-AG isolated from (Ala-Hyp)(51)-green fluorescent pro

60 cently we described the structure of a small Hyp-AG putatively based on repetitive trigalactosyl subu

61 hat is, a 22-residue Hyp-AG and a 20-residue Hyp-AG, both isolated from interferon alpha2b-(Ser-Hyp)(

62 The Hyp-AGs were isolated from two different arabinogalactan

63 ed by type II O-Hyp-linked arabinogalactans (Hyp-AGs) are structural components of the plant extracel

64 o establish the molecular structure of these Hyp-AGs, which share common features: (i) a galactan mai

65 Their complete sequences (gld-V/gld-V' = Ala-Hyp-Ala-Asn-Ser-d-Hyv-Trp-Ser and mus-V/mus-V' = Ser-Hyp

66 Asn-Ser-d-Hyv-Trp-Ser and mus-V/mus-V' = Ser-Hyp-Ala-Asn-Ser-d-Hyv-Trp-Ser) were determined by a comb

67 onfirmed at 12 weeks was 16% with daclizumab HYP and 20% with interferon beta-1a (P=0.16).

68 accomplish this, expression profiles of 1234 HyP and conserved genes were used from transcriptomic da

69 NA expression (129- and 124-fold increase in Hyp and Dmp1(-/-) vs. 1.3-fold in Hyp+SU5402 and 2.5-fol

70 Moreover, treatment of Hyp and FGF23R1760-transgenic mice with the CYP24 inhibi

71 ix conformation and stabilisation (Gly, Pro, Hyp and Hyl), whilst the Lys content was greater for the

72 We generated Hyp and klotho double-mutant mice (Hyp/klotho(-/-)).

73 tect bone sialoprotein (BSP) distribution in Hyp and WT mouse molar tissues, and transmission electro

74 Hypothetical (HyP) and conserved HyP genes account for >30% of sequenc

75 XLH and phenotypes of wild-type, Mepe(-/-), Hyp, and Mepe(-/-)/Hyp mice were examined.

76 lpha-ImI were synthesized with either Pro or Hyp, and their in vitro oxidative folding and biological

77 e, inhibiting FGFR signaling using SU5402 in Hyp- and Dmp1(-/-)-derived bone marrow stromal cells pre

78 ly characterized by the presence of [Alanine-Hyp] ([AO]) repetitive units.

79 09 m [5280 feet]), and severe high altitude (HYP) ( approximately 5182 m [ approximately 17,000 feet]

80 7 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-beta1,4Araf-beta1,2Araf-bet

81 f) side chains in an alpha-linkage, to yield Hyp-Araf4 which is exclusively found in extensins.

82 n the Viridiplantae along with its' product, Hyp-Araf4, point to ExAD being an evolutionary hallmark

83 tification of the catabolic pathway in which Hyp-B 2-epimerase participates.

84 transfer of Araf to Hyp-arabinofuranotriose (Hyp-beta1,4Araf-beta1,2Araf-beta1,2Araf) side chains in

85 gen triple-helix stability in the absence of Hyp, biophysical studies were carried out on recombinant

86 pyrophosphate concentration is increased in Hyp bones, and that Tnap expression is decreased in Hyp-

87 terminal, central and C-terminal beta-l-Araf-Hyp building blocks.

88 was shown to carry Gal residues on isolated Hyp but also Ara residues.

89 eplacement of ProB28 by (4R)-hydroxyproline (Hyp) causes little change in the rates of fibrillation a

90 Formation of oversized Hyp clusters has been observed on the GUV surface at pro

91 he vesicle diameter and the buildup of large Hyp clusters in the GUV membrane.

92 neralization of the nasal septum in Mgp(-/-);Hyp compound mutants.

93 hree-dimensional solution structure of a Gla/Hyp-containing 18-residue conantokin, conRl-B, by high f

94 ional hydrogen-bonding capacity, the Pro-->2-Hyp conversion alters the active site and enhances signi

95 hepatitis; a contributory role of daclizumab HYP could not be excluded.

96 competition from PDH reduced anaplerosis in HYP+DCA by 18%.

97 Interestingly, reduced anaplerosis in HYP+DCA corresponded with normalized TAG (14.9+/-0.6 mic

98 ctivation: HYP=1419+/-220 nmol/g dry weight; HYP+DCA=343+/-56 nmol/g dry weight.

99 idence of osteomalacia; however, cultures of Hyp-derived bone marrow stromal cells in the absence of

100 Hyp-derived osteocyte-like cells but not in Hyp-derived osteoblasts ex vivo and in vitro.

101 es, and that Tnap expression is decreased in Hyp-derived osteocyte-like cells but not in Hyp-derived

102 and decreased pyrophosphate concentration in Hyp-derived osteocyte-like cells in vitro.

103 The Hyp diastereomer (2S,4S)-4-hydroxyproline (hyp) has not

104 t and re-initiation group (300 mg daclizumab HYP) died because of autoimmune hepatitis; a contributor

105 Even though its product (Hyp) differs from its substrate (Pro) by only a single o

106 ween the hydroxyl moiety and the carbonyl of hyp distorts the main-chain torsion angles that typicall

107 o single-mutant littermates, compound-mutant Hyp/Dmp1(-/-) mice displayed nonadditive elevations of s

108 otype of compound Phex and Dmp1 mutant mice (Hyp/Dmp1(-/-)).

109 ind that the 4S-hydroxylation of Pro to form hyp does indeed enforce a C(gamma)-endo ring pucker but

110 riodicity: SOOOOKKHYVYKSOOOOVKHYSOOOVYH (O = Hyp), each repeat containing a hydrophobic isodityrosine

111 s conformation of the peptide bond preceding hyp, endowing hyp with the unusual combination of a C(ga

112 ive 150 mg or 300 mg subcutaneous daclizumab HYP every 4 weeks for 52 weeks (treatment initiation gro

113 Another isoform, HSSTnT hypothetical (Hyp) (-exon 5, +exon 12), has only been found at the mRN

114 The time evolution of Hyp fluorescence originating from Hyp monomers dissolved

115 lts support further assessment of daclizumab HYP for relapsing-remitting multiple sclerosis.

116 who received daclizumab high-yield process (HYP) for 52 weeks.

117 lphaKG)-dependent dioxygenases that catalyze Hyp formation.

118 peptide (denoted as the Hyp minus peptide or Hyp-) forms a rod-like triple helix structure without an

119 The incorporation of hypericin (Hyp) from aqueous solutions into giant unilamellar vesic

120 -radiolabeled fragment that co-eluted with a Hyp-Gal standard after high performance anion-exchange c

121 Examination of the Arabidopsis Hyp:GalT activity using various acceptor substrates, inc

122 tated as conserved HyP (9.5%) along with 887 HyP genes (24.4%).

123 Hypothetical (HyP) and conserved HyP genes account for >30% of sequenced bacterial genome

124 siRNA knockdown of human orthologs of the hyp genes conferred hypoxia resistance to transformed hu

125 The hyp genes had significant overlap with previously identi

126 Eighty-six per cent of these hyp genes had strong homologs in other organisms, 73 wit

127 croarray analysis identified a subset of the hyp genes that may be hypoxia regulated.

128 The hyp genes were distributed among multiple functional cat

129 e radial assembly, H-(byp)(2) containing Pro-Hyp-Gly repeating sequences and two staggered bipyridine

130 folding in collagen peptides composed of Pro-Hyp-Gly triplet repeats, allowing for truncation to the

131 The two Pro-Hyp-Gly zones adopt the typical triple-helical collagen

132 h a sequence comprising a central block (Pro-Hyp-Gly) and two positively charged domains (Pro-Arg-Gly

133 After self-association, (Pro-Hyp-Gly)(10) forms branched filamentous structures, in c

134 The triple helical form of (Pro-Hyp-Gly)(10), a peptide that has proved a useful model f

135 vious analyses have revealed that H-(Pro-4(R)Hyp-Gly)(10)-OH forms a stable triple helix, whereas H-(

136 amino acids on the self-association of (Pro-Hyp-Gly)(10).

137 lar kind of interruption in the peptide (Pro-Hyp-Gly)10, (GPOGPOPOGPO), is unable to form a stable tr

138 Gly)3 and negatively charged C-terminal (Glu-Hyp-Gly)3 triad extensions, respectively.

139 he collagen model peptide with sequence (Pro-Hyp-Gly)4-Pro-Gly-(Pro-Hyp-Gly)5 contains a central Gly-

140 de with sequence (Pro-Hyp-Gly)4-Pro-Gly-(Pro-Hyp-Gly)5 contains a central Gly-Pro-Gly interruption in

141 (68)Ga-P03034 ((68)Ga-DOTA-dPEG2-Lys-Arg-Pro-Hyp-Gly-Cha-Ser-Pro-Leu) in B1R-positive (B1R+) HEK293T:

142 nd Z02090 ((68)Ga-DOTA-dPEG2-Lys-Lys-Arg-Pro-Hyp-Gly-Cpg-Ser-D-Tic-Cpg) derived from 2 potent B1R ant

143 ed P04158 ((68)Ga-DOTA-dPEG2-Lys-Lys-Arg-Pro-Hyp-Gly-Igl-Ser-D-Igl-Oic) and Z02090 ((68)Ga-DOTA-dPEG2

144 T(m) value equal to that seen for a Gly-Pro-Hyp-Gly-Pro-Hyp sequence.

145 tere, Ac-(Gly-Pro-Hyp)3-Gly-psi[(E)CH C]-Pro-Hyp-(Gly-Pro-Hyp)4-Gly-Gly-Tyr-NH2, had a Tm value of 28

146 inofuranosylated hydroxyproline (beta-l-Araf-Hyp) glycocluster is described.

147 ted in 15% of the patients in the daclizumab HYP group and in 10% of those in the interferon beta-1a

148 nfections were more common in the daclizumab HYP group than in the interferon beta-1a group (in 65% v

149 HYP had elevated ME expression with a 90% elevation in a

150 As Hyp has a strong preference for C (gamma)- exo pucker an

151 e Hyp diastereomer (2S,4S)-4-hydroxyproline (hyp) has not been observed in a protein, despite the abi

152 In an automated manner, the Hyp hydroxyl is protected and the remainder of the pepti

153 ollagen peptide shows Phe interacts with Pro/Hyp in a neighboring triple-helical molecule.

154 ated this activity was specific for peptidyl Hyp in AGP sequences.

155 ensor makes it unique for easy estimation of Hyp in collagen and biological samples.

156 dress this disparity, as well as the role of Hyp in conantokins, we have solved the high resolution t

157 In contrast, the presence of Hyp in MVIIC had a significant impact on the oxidative f

158 Notably, however, the 4(R)Hyp in the Xaa position also takes the up pucker, which

159 In certain contexts, triple helices with Hyp in the Xaa position are now known to be hyperstable.

160 Thus, the presence of 3(S)Hyp in the Xaa positions does not lead to large structur

161 s have demonstrated that the presence of 3(S)Hyp in the Xaa positions of collagen-like peptides actua

162 The puckering of the Yaa position 4(R)Hyp in this structure is up (Cgamma exo), as has been fo

163 or adding galactose (Gal) to hydroxyproline (Hyp) in AGP protein backbones.

164 vestigated the function of 4-hydroxyproline (Hyp) in conotoxins from three distinct gene families: mu

165 d calorimetry to explore the consequences of hyp incorporation on protein stability using a collagen

166 y-Asp-Lys motifs replaced Gly-Pro-Hyp (where Hyp is 4-hydroxy-L-proline) repeats.

167 Hyp is a major contributor to triple-helix stability in

168 Daclizumab high-yield process (HYP) is a humanized monoclonal antibody that binds to CD

169 he resulting product trans-4-hydroxyproline (Hyp) is of critical importance for the stability and thu

170 to be excellent substrates for P4H, forming Hyp, Kep, and (2 S,4 R)-thiaoxoproline, respectively.

171 yp mice was reversed to hyperphosphatemia in Hyp/klotho(-/-) double mutants, despite the fact that th

172 Moreover, in contrast to the Hyp mice, the Hyp/klotho(-/-) mice showed significantly higher serum l

173 Hyperphosphatemia in Hyp/klotho(-/-) mice was associated with increased renal

174 Furthermore, compared with the Hyp mice, Hyp/klotho(-/-) mice were smaller in size, showed featur

175 quently reduced serum levels of phosphate in Hyp/klotho(-/-) mice.

176 generated Hyp and klotho double-mutant mice (Hyp/klotho(-/-)).

177 ctan proteins partially defined by type II O-Hyp-linked arabinogalactans (Hyp-AGs) are structural com

178 or the trans-conformation in the order Pro < Hyp < [alpha-(1,4)GlcNAc]Hyp.

179 ved for Scl2 was similar to the contribution Hyp makes to the stability of mammalian collagens.

180 3 levels and concomitant hypophosphatemia in Hyp mice and to evaluate whether FGF23 activity could be

181 e results demonstrate that MEPE elevation in Hyp mice does not contribute to the hypophosphatemia ass

182 Dentin in Hyp mice exhibited mineralization defects by 33 dpc, as

183 The authors found that Hyp mice had increased expression of the MEPE and anothe

184 Such urinary phosphate wasting in Hyp mice is associated with an increased serum accumulat

185 Hyp mice possess a mutation that inactivates the phospha

186 In vivo, bone-specific deletion of Fgf23 in Hyp mice rescued the suppressed TNAP activity in osteocy

187 In addition, Mepe-deficient Hyp mice retained bone mineralization defects in vivo, c

188 Severe hypophosphatemia of Hyp mice was reversed to hyperphosphatemia in Hyp/klotho

189 of FGFR3 in mediating the effects of FGF23, Hyp mice were crossed with FGFR3-null mice; interestingl

190 of wild-type, Mepe(-/-), Hyp, and Mepe(-/-)/Hyp mice were examined.

191 Increased Fgf23 levels in Hyp mice were not affected by superimposed Mepe deficien

192 Hyp mice, a murine homologue of XLH, are characterized b

193 Furthermore, compared with the Hyp mice, Hyp/klotho(-/-) mice were smaller in size, sho

194 emia and reduced vitamin D hormone levels in Hyp mice, its putative role as an auto-/paracrine osteom

195 Moreover, in contrast to the Hyp mice, the Hyp/klotho(-/-) mice showed significantly

196 ecifically, we evaluated Cyp24 deficiency in Hyp mice, the murine homolog of X-linked dominant hypoph

197 Compared with wild-type mice, Hyp mice, which have elevated circulating levels of FGF2

198 2 protein in osteoblasts and osteocytes from Hyp mice.

199 ein in nuclear fractions from osteoblasts of Hyp mice.

200 regulating abnormal phosphate homeostasis in Hyp mice.

201 study molar tissues from wild-type (WT) and Hyp mice.

202 he aforementioned metabolic abnormalities of Hyp mice.

203 4 also failed to correct hypophosphatemia in Hyp mice.

204 recombinant soluble Klotho lowered BP in the Hyp mice.

205 he suppressed TNAP activity in osteocytes of Hyp mice.

206 contributing to the mineralization defect in Hyp mice.

207 fr3 mRNA in osteocytes versus osteoblasts of Hyp mice.

208 t in the teeth of X-linked hypophosphatemic (Hyp) mice.

209 gene and mutations in affected patients and hyp-mice established that alterations in PHEX/Phex expre

210 PhexDeltaflox/y, OC-Cre-PhexDeltaflox/y, and hyp-mice manifested comparable osteomalacia.

211 PhexDeltaflox/y, OC-Cre-PhexDeltaflox/y, and hyp-mice was likewise reduced compared with that in norm

212 PhexDeltaflox/y, OC-Cre-PhexDeltaflox/y, and hyp-mice were lower than those in normal mice.

213 te cotransporter protein, as was the case in hyp-mice.

214 The peptide (denoted as the Hyp minus peptide or Hyp-) forms a rod-like triple helix

215 ow the aggregation/dissociation processes of Hyp molecules in the membrane.

216 olution of Hyp fluorescence originating from Hyp monomers dissolved in the GUV membrane has been reco

217 r of Mepe deficiency onto the Phex-deficient Hyp mouse background failed to correct hypophosphatemia

218 formation, a phenotype recapitulated in the Hyp mouse homolog.

219 pe-deficient mice were back-crossed onto the Hyp mouse homologue of XLH and phenotypes of wild-type,

220 d BP and significantly attenuated LVH in the Hyp mouse model of excess FGF-23, but did not induce a r

221 velopmental study of tooth root formation in Hyp mouse molars, focusing on dentin and cementum.

222 in the phosphate-regulating Phex gene of the Hyp mouse resulted in defective cementum development.

223 ether HMW FGF2 expression was altered in the Hyp mouse, a mouse homolog of the human disease X-linked

224 In the Hyp-mouse homologue of X-linked hypophosphatemic rickets

225 lineage cells, transgenic Phex expression in hyp-mouse osteoblasts fails to rescue the phenotype, sug

226 teocytes alone is sufficient to underlie the hyp-mouse phenotype.

227 with X-linked hypophosphatemia (XLH) and the hyp-mouse, a model of XLH characterized by a deletion in

228 sequences like (Gly-Pro-Pro)(n) or (Gly-Pro-Hyp)(n).

229 The existence of new type Hyp-O-Gal/Ara-rich motifs not recognized by the beta-glu

230 ls because arabinogalactans could be absent, Hyp-O-Gal/Ara-rich motifs of different sizes were observ

231 aa is often proline and Yaa, hydroxyproline (Hyp/O).

232 tural collagen, 3(S)-hydroxyl-L-proline (3(S)Hyp) occurs in the Xaa positions to varying extents and

233 ession was also suppressed in the RN, FC and HYP of females and RN of dominant pigs (P < 0.05).

234 oration of [(14)C]Gal from UDP-[(14)C]Gal to Hyp of model substrate acceptors having AGP peptide sequ

235 etramer in the ER, we discuss the effects of Hyp on the folding of conotoxins in the context of cis-t

236 year of continuous treatment with daclizumab HYP or during treatment washout and re-initiation.

237 ll autonomous increase in Fgf23 secretion in Hyp osteocytes drives the accumulation of pyrophosphate

238 54% lower number of lesions with daclizumab HYP; P<0.001).

239 .22 vs. 0.39; 45% lower rate with daclizumab HYP; P<0.001).

240 blasts and/or osteocytes alone generates the HYP phenotype, we created mice with a global Phex knocko

241 forms a stable triple helix, whereas H-(4(R)Hyp-Pro-Gly)(10)-OH does not.

242 After peptide synthesis, the Hyp protecting group is orthogonally removed and Hyp sel

243 ed an invariant hypoxia-resistant phenotype (Hyp-r).

244 of non-contiguous Hyp residues, such as (Ala-Hyp) repetitive units exemplified by chemically synthesi

245 de molecule to the C-terminal or penultimate Hyp residue of the [AO](7) peptide.

246 he varphi/psi dihedral angles of the Xaa 3(S)Hyp residues are also similar to those of typical collag

247 normal triple helical structure for Gly-Pro-Hyp residues flanking the break.

248 on of a stable triple helix, the role of 3(S)Hyp residues in the Xaa position is not well understood.

249 to determine what effect the presence of 3(S)Hyp residues in the Xaa positions has on the overall con

250 Although 4(R)Hyp residues in the Yaa positions have been shown to be

251 Given the high fraction of Pro and Hyp residues on the surface of collagen molecules, it is

252 amidoproline were overlaid with the Pro and Hyp residues within a crystal structure of collagen reve

253 romatic residues in the telopeptides and Pro/Hyp residues within the triple helix.

254 tide sequences, consisting of non-contiguous Hyp residues, such as (Ala-Hyp) repetitive units exempli

255 For GIIIA, which naturally contains three Hyp residues, the modifications improved the ability to

256 shows that, despite the presence of the 3(S)Hyp residues, the peptide still adopts a typical 7/2 sup

257 tive and powerful: it permitted us to locate Hyp residues, to demonstrate the presence of carbohydrat

258 The puckering of the Xaa position 3(S)Hyp residues, which are all down (Cgamma-endo), and the

259 h much more conformational stability than do hyp residues.

260 lpha1 chain of type I collagen containing no Hyp (residues 877-939) obtained from Escherichia coli.

261 The resulting (2 S,4 R)-4-hydroxyproline (Hyp) residues are essential for the folding, secretion,

262 The resulting (2S,4R)-4-hydroxyproline (Hyp) residues are essential for the folding, secretion,

263 oline (Pro) and 4(R)-hydroxyl-L-proline (4(R)Hyp) residues are found most frequently in the Xaa and Y

264 ly proline and (2S,4R)-4-hydroxyproline (4(R)Hyp), respectively.

265 ommonly proline (Pro) and 4R-hydroxyproline (Hyp), respectively.

266 Among them, hydroxyproline (Hyp)-rich glycoproteins constitute a complex family of O

267 GPs) are highly glycosylated hydroxyproline (Hyp)-rich glycoproteins that are frequently characterize

268 expressed, secreted protein with homology to Hyp-rich cell wall proteins.

269 In this study, we characterized an atypical Hyp-rich glycoprotein, AGP31 (arabinogalactan protein 31

270 ndergo sexual adhesion via enormous chimeric Hyp-rich glycoproteins (HRGPs), the plus and minus sexua

271 In contrast, the presence of the Hyp-rich sequence GPO(GAO)(3) N-terminal to the mutation

272 ally through substitution by hydroxyproline (Hyp), (S)-beta-homoproline (betaPro), 2-aminocyclopenten

273 In contrast, we found in HYP seizures that (1) fast ripple rates increased during

274 and region-specific patterns during LVF and HYP seizures, thus suggesting that they play specific ro

275 fast-onset (LVF) and hypersynchronous-onset (HYP) seizures in the rat pilocarpine model of temporal l

276 protecting group is orthogonally removed and Hyp selectively modified to generate substituted proline

277 equal to that seen for a Gly-Pro-Hyp-Gly-Pro-Hyp sequence.

278 d in the presence of Gly-Pro-Lys-Gly-Asp/Glu-Hyp sequences, leading to a T(m) value equal to that see

279 nd with high affinity to tandem GPO (Gly-Pro-Hyp) sequences in collagen, whereas the markedly lower a

280 Hyp should prevent adoption of the metal ion-induced ful

281 HYP showed a 13% to 26% reduction in rate pressure produ

282 ing-remitting multiple sclerosis, daclizumab HYP showed efficacy superior to that of interferon beta-

283 A few extensin-like motifs with contiguous Hyp (SOOA and SOOT) were also found.

284 ncrease in Hyp and Dmp1(-/-) vs. 1.3-fold in Hyp+SU5402 and 2.5-fold in Dmp1(-/-)+SU5402, P<0.05).

285 respondingly, malate was 2.2-fold greater in HYP than SHAM but was lowered with PDH activation: HYP=1

286 lized relapse rate was lower with daclizumab HYP than with interferon beta-1a (0.22 vs. 0.39; 45% low

287 period of 96 weeks was lower with daclizumab HYP than with interferon beta-1a (4.3 vs. 9.4; 54% lower

288 function testing were higher with daclizumab HYP than with interferon beta-1a.

289 O-Methylation of hyp to form (2S,4S)-4-methoxyproline (mop) eliminates th

290 se the multistep conversion of hypoxanthine (Hyp) to dGMP for DNA synthesis.

291 es (GF, GY) are present, and a rigid Gly-Pro-Hyp tripeptide adjacent to the interruption leads to gre

292 ns, identifying common residues such as Pro, Hyp, Tyr, and Ala.

293 o process is used to cleave the customizable Hyp unit under mild, metal-free conditions.

294 In a model tetrapeptide (Ac-TYPN-NH2), 4R-Hyp was stereospecifically converted to 122 different 4-

295 Most Hyp were isolated within repeated motifs such as KAOV, K

296 on group); those who had received daclizumab HYP were randomly assigned (1:1) to continue their prese

297 AMY), frontal cortex (FC), and hypothalamus (HYP) were dissected; relative mRNA abundance for 5-HT(B)

298 perated (SHAM) and aortic banded rat hearts (HYP) were perfused with buffer containing either 13C-pal

299 y-Asp or Gly-Asp-Lys motifs replaced Gly-Pro-Hyp (where Hyp is 4-hydroxy-L-proline) repeats.

300 of the peptide bond preceding hyp, endowing hyp with the unusual combination of a C(gamma)-endo ring