ライフサイエンスコーパス: I-kappa B

1 I kappa B alpha phosphorylation is a required step in th

2 I kappa B alpha preferentially binds to the p50/p65 hete

3 I kappa B alpha retains inactive NF-kappa B dimers in th

4 I kappa B alpha was detected in the frontal cortex of no

5 I kappa B alpha(Delta N)-transgenic (Tg) mice succumbed

6 I kappa B alpha, an NF-kappa B inducible and constitutiv

7 I kappa B kinase (IKK) is the converging point for the a

8 I kappa B protein expression was determined by Western b

9 I kappa B proteins are widely expressed in different cel

10 I kappa B, inhibitor of apoptosis protein (IAP)-1, and c

11 I kappa B-alpha and I kappa B-beta proteins were degrade

12 I-kappa B kinase (IKK) is a serine/threonine kinase that

13 I-kappa-B kinase e (IKBKE; IKKepsilon) has been recently

14 gnaling pathway involving I kappa B kinase 1/I kappa B kinase alpha (IKK1/IKKalpha) and NF-kappaB-ind

15 kappa B, by a kinase complex that contains 2 I kappa B kinases.

16 lation defective I kappa B alpha (S32, 36A) (I kappa B alpha M) that blocks NF-kappa B activity.

17 does indeed abolish its ability to activate I kappa B-kinase (IKK) complexes, but paradoxically it a

18 sactivated, followed by p38 MAPK activation, I-kappa B alpha (IkappaBalpha) degradation, NFkappaB act

19 tion of Akt is, however, detected only after I kappa B-alpha degradation is induced by these agents.

20 ase (PI3K), the serine/threonine kinase Akt, I kappa B kinases, and finally nuclear factor NF-kappa B

21 he phosphatidylinositol 3' kinase (PI3K)/AKT/I kappa B kinase (IKK) pathway positively regulates NF k

22 on site-deficient mutant of I kappa B alpha (I kappa B alpha M, S32/36A) was introduced into EMT6 cel

23 horylation of inhibitor of NF-kappa B alpha (I kappa B alpha) and NF-kappaB nuclear binding activity.

24 d abundance of inhibitor of NF-kappaB alpha (I kappa B-alpha) and I kappa B-beta.

25 An I kappa B phosphorylation inhibitor, Bay 11-7082, inhibi

26 r cells with tumor necrosis factor-alpha, an I kappa B kinase activator, did not alter the suppressiv

27 MLK3 has also been implicated as an I kappa B kinase kinase in the activation of NF-kappa B.

28 and a loss-of-function mutant of Cactus, an I kappa B-like factor that inhibits the Toll signaling,

29 y detecting changes in phosphorylation of an I kappa B alpha-glutathione S-transferase (GST) fusion p

30 059 on p50 homodimer activity, suggesting an I kappa B kinase-independent pathway for p50 homodimer a

31 yrrolidinedithiocarbamate as well as with an I kappa B alpha dominant negative TAT fusion protein.

32 radation of the NF-kappa B cytosolic anchor, I kappa B, as well as the translocation of the p65 subun

33 ing to DNA, NF-kappa B trans-activation, and I kappa B degradation.

34 est that low but persistent IKK activity and I kappa B degradation lead to prolonged NF-kappa B nucle

35 f NF-kappa B-inducing kinase (NIK), Akt, and I kappa B kinase (IKK) were not changed.

36 rotrimeric G proteins, PI3K, PDK-1, Akt, and I kappa B kinase (IKK).

37 G-protein activation through PI3K, Akt, and I kappa B kinase to NF-kappa B activation.

38 B proteins, among which I kappa B alpha and I kappa B beta are the most abundant.

39 alysis of the ability of I kappa B alpha and I kappa B beta to serve as substrates for IKKs and help

40 degradation kinetics of I kappa B alpha and I kappa B beta.

41 ubunits to phosphorylate I kappa B alpha and I kappa B beta.

42 tent with c-Rel, whereas I kappa B-alpha and I kappa B-beta associate with Rel A only.

43 I kappa B-alpha and I kappa B-beta proteins were degraded during this time,

44 tor of NF-kappaB alpha (I kappa B-alpha) and I kappa B-beta.

45 We report here that NF-kappa B and I kappa B alpha (but not I kappa B beta) are also locali

46 otential role for a subset of NF-kappa B and I kappa B family proteins, particularly NF-kappa B/p52 a

47 ncluded pleckstrin, Jun-B, c-FLIP, CIP4, and I kappa B epsilon.

48 typically terminated by desensitization, and I kappa B-alpha reappearance normally occurs within 30-6

49 the help of the C-terminal death domain and I kappa B kinase alpha-targeting serines, the C-terminal

50 B luciferase activity, IL-6 production, and I kappa B alpha degradation elicited by LPS and IL-1 bet

51 vel signal in postreplicative DNA repair and I kappa B alpha kinase activation.

52 proteins were degraded during this time, and I kappa B-alpha was resynthesized subsequently by NF-kap

53 required for p65-mediated transcription and I kappa B alpha expression in fibroblasts.

54 nase that phosphorylates I-kappa B alpha and I-kappa B beta and targets them for polyubiquitination a

55 Interference with cell cycle progression and I-kappa B kinase (IKK)/nuclear factor kappa B (NF-kappaB

56 ing activity by electromobility shift assay, I kappa B degradation by Western blotting, and trypsinog

57 sphorylation of the inhibitor of NF-kappa B (I kappa B alpha).

58 B blockade by mutant inhibitory (I)-kappa B (I kappa B) affects TNF-alpha-induced cell death.

59 A binding activity and inhibitor of kappa B (I kappa B) phosphorylation was dramatically decreased in

60 tivity is regulated by inhibitor of kappa B (I kappa B), the gene encoding I kappa B-alpha was disrup

61 -homology domains and the inhibitor kappa B (I kappa B)-like domain, which is similar to Drosophila R

62 ransfer of the superrepressor of NF-kappa B, I kappa B-2A, blocks basic fibroblast growth factor-indu

63 In the NF-kappa B-I kappa B autoregulatory loop, activated (nuclear) Rel A

64 subunit is a major contributor to NF-kappa B.I kappa B alpha complex formation.

65 ssed among the embryonic tissues, NF-kappa B/I kappa B members present distinct patterns of gene expr

66 conservation and compatibility of NF-kappa B/I kappa B proteins among vertebrates and the importance

67 e cloning and characterization of NF-kappa B/I kappa B proteins in zebra fish.

68 revious studies have shown an elevated basal I kappa B kinase (IKK) activity in Hs294T melanoma cells

69 Because I kappa B kinase (IKK) is critical in transducing the si

70 furcation of NTHi-induced NIK-IKK alpha/beta-I kappa B alpha and MKK3/6--p38 MAP kinase pathways may

71 via TLR2-TAK1-dependent NIK--IKK alpha/beta-I kappa B alpha and MKK3/6--p38 MAP kinase signaling pat

72 tudies have demonstrated that, although both I kappa B proteins are phosphorylated by the same I kapp

73 Finally, both I kappa B alpha and p65 were comparable substrates for I

74 ak kinase for the N-terminal serines of both I kappa B isoforms, IKK beta is an efficient kinase for

75 he NF-kappa B signaling pathway through both I kappa B degradation and RelA phosphorylation but that

76 The reduction of brain I kappa B alpha levels was paralleled by increased infla

77 rophages and dendritic cells are normal, but I kappa B-alpha homeostatic regulation and NF-kappa B ac

78 The inhibition of NF-kappa B by I kappa B alpha, a natural inhibitor of NF-kappa B, supp

79 tor whose nuclear residence is controlled by I kappa B family members.

80 osphorylated and targeted for degradation by I kappa B kinases (IKK alpha and IKK beta).

81 In vascular endothelial cells, I kappa B-epsilon associates predominantly with the NF-k

82 lated with its ability to stabilize cellular I kappa B protein.

83 imulated after exposure to TSNs, cytoplasmic I kappa B alpha persisted as a result of alterations in

84 ranslocation into the nucleus, and decreased I kappa B protein expression.

85 n blot analysis showed that injury decreased I kappa B protein expression.

86 nes that express a phosphorylation defective I kappa B alpha (S32, 36A) (I kappa B alpha M) that bloc

87 -1 receptor-associated kinase-1 degradation, I kappa B alpha phosphorylation/degradation, RelA and Ak

88 plex into lipid rafts, activate IKK, degrade I kappa B or up-regulate NF-kappa B-dependent survival s

89 V-tat protein activated NF-kappa B, degraded I kappa B alpha, and induced NF-kappa B-dependent report

90 H(2)O(2) treatment degraded I kappa B(beta), but not I kappa B(alpha), to translocat

91 kappa B nuclear translocation and diminished I kappa B alpha degradation.

92 or of kappa B (I kappa B), the gene encoding I kappa B-alpha was disrupted in mice to observe the in

93 NF-kappa B activity correlated with enhanced I kappa B degradation.

94 nhibiting NF-kappa B signaling by expressing I kappa B alpha M significantly reduced expression of Bc

95 nstitutive NF-kappa B activity by expressing I kappa B alpha M suppressed the tumorigenicity of a non

96 We show that zebra fish I kappa B alphas are degraded in a time-dependent manner

97 the slower rate of degradation observed for I kappa B beta.

98 removes Lys-63-linked ubiquitin chains from I kappa B kinase signaling components and thereby inhibi

99 ing expression of the PPAR alpha target gene I kappa B alpha, although only in the genetic presence o

100 ion, which is, in turn, because of a greater I kappa B degradation in old M phi.

101 tivation in old M phi was because of greater I kappa B degradation in the cytoplasm and p65 transloca

102 ed both TNF-alpha- and IL-1 beta-induced GST-I kappa B and GST-p65 phosphorylation, I kappa B degrada

103 s the capacity to directly phosphorylate GST-I kappa B alpha and that this I kappa B alpha kinase act

104 However, I kappa B isoforms and IKK activity did not return compl

105 familial dysautonomia patients with IKBKAP (I-kappa-B kinase complex-associated protein) mutation co

106 ns are able to rescue NF-kappa B activity in I kappa B alpha(-/-) MEFs.

107 lpha persisted as a result of alterations in I kappa B kinase (IKK) activity.

108 e was a delay but not a complete blockade in I kappa B alpha phosphorylation and degradation; likewis

109 on with TNF-alpha induced a >10x increase in I kappa B kinase (IKK) activity that quickly diminished

110 This increase in I kappa B-alpha phosphorylation and degradation leads to

111 nes, demonstrated a significant reduction in I kappa B alpha tyrosine phosphorylation and NF kappa B

112 pa B DNA binding activity and a reduction in I kappa B kinase (IKK) activity.

113 is an efficient kinase for those residues in I kappa B alpha.

114 Single genes of interest included I-kappa-B kinase-alpha (CHUK), and a phosphorylation dep

115 de of nuclear factor (NF)-kappa B, including I kappa B kinase and nuclear translocation of NF-kappa B

116 NE increased I kappa B alpha protein in both the cytosolic and the nu

117 Indeed, I kappa B kinase, the kinase involved in NF-kappa B acti

118 crosis factor (TNF) and interleukin-1 induce I kappa B-alpha phosphorylation, leading to I kappa B-al

119 CXCL16 induced I kappa B phosphorylation and degradation.

120 kappa B expression blocked cytokine-induced I kappa B degradation and NF-kappa B transcriptional act

121 Celecoxib inhibited TNF-induced I kappa B alpha kinase activation, leading to suppressio

122 Of importance, B. vulgatus induced I kappa B alpha phosphorylation/degradation and IKK alph

123 Interestingly, B. vulgatus-induced I kappa B alpha degradation and NF-kappa B transcription

124 xpression may be a combination of inhibiting I kappa B alpha phosphorylation/degradation, affecting N

125 d blocks degradation of NF-kappa B inhibitor I kappa B alpha by the ubiquitin-proteasome pathway with

126 The NF-kappa B inhibitor I kappa B-epsilon is a new member of the I kappa B prote

127 ed through phosphorylation of its inhibitor, I kappa B, by a kinase complex that contains 2 I kappa B

128 uitin-mediated degradation of its inhibitor, I kappa B.

129 presence of a mutated NF-kappa B inhibitor, I-kappa B alpha.

130 s DNA-binding (kappa B) sites and inhibitory I kappa B alpha proteins from mammals.

131 ently increased the expression of inhibitory I kappa B alpha protein accompanied by an increase in st

132 d through coordinate induction of inhibitory I kappa B molecules to restore cellular homeostasis.

133 diated via phosphorylation of the inhibitory I kappa B proteins by the I kappa B kinase complex (IKK)

134 lated by its association with the inhibitory I kappa B proteins, among which I kappa B alpha and I ka

135 ppaB (NF-kappaB) signaling pathway involving I kappa B kinase 1/I kappa B kinase alpha (IKK1/IKKalpha

136 NE activated a 1.3-kilobase I kappa B alpha promoter transfected into astrocytes or

137 KK alpha was not required for RANKL-mediated I kappa B alpha degradation or phosphorylation of mitoge

138 eads to the phosphorylation of mitochondrial I kappa B alpha and its subsequent degradation by a nonp

139 The multisubunit I kappa B kinase responsible for this phosphorylation co

140 on of a dominant-negative form of the murine I kappa B alpha (mI kappa B alpha M), which is able to b

141 Mutant I kappa B expression blocked cytokine-induced I kappa B

142 by TNF-alpha or mutant I kappa B, and mutant I kappa B abolished TNF-alpha-induced c-FLIP induction i

143 In contrast, mutant I kappa B alpha was unable to inhibit radioresistance or

144 encoding either beta-galactosidase or mutant I kappa B and then treated with TNF-alpha or interleukin

145 sion was not affected by TNF-alpha or mutant I kappa B, and mutant I kappa B abolished TNF-alpha-indu

146 nhibitors and a degradation-resistant mutant I kappa B-alpha demonstrated that the transcription fact

147 l activity in MCF+FIR cells, by using mutant I kappa B alpha, inhibited radioresistance as well as re

148 f array elements that decreased with Delta N-I kappa B alpha expression decreased with BAY11 treatmen

149 to those of an NF-kappa B inhibitor, Delta N-I kappa B alpha, in effecting decreased JNK1 expression

150 In contrast, dominant-negative-I-kappa B reversed the effects of DENN-SV, but not IG20,

151 that NF-kappa B and I kappa B alpha (but not I kappa B beta) are also localized in the mitochondria.

152 treatment degraded I kappa B(beta), but not I kappa B(alpha), to translocate NF-kappa B to the nucle

153 ible phosphorylation of serines 32 and 36 of I kappa B alpha is critical in regulating the subsequent

154 ibitor corresponding to amino acids 26-42 of I kappa B alpha with Ser-32 and Ser-36 changed to aspart

155 first systematic analysis of the ability of I kappa B alpha and I kappa B beta to serve as substrate

156 up+ cells resulted in a slight activation of I kappa B kinase and in enhanced NF-kappa B DNA binding

157 The elevated basal activity of I kappa B kinase in macrophages from Ucp2-/- mice can be

158 Finally, we examined the kinase activity of I kappa B kinase-alpha and found that this kinase, unlik

159 F-R and represent previously unknown arms of I kappa B kinase (IKK)1-dependent signaling.

160 a-induced phosphorylation and degradation of I kappa B alpha as well as the consequent activation of

161 S induces phosphorylation and degradation of I kappa B alpha, leading to the nuclear translocation of

162 own to be responsible for the degradation of I kappa B and the subsequent activation of NF-kappa B.

163 OS, activation of IKK kinase, degradation of I kappa B beta, and subsequent activation of NF-kappa B.

164 The degradation of I kappa B is controlled initially by phosphorylation ind

165 The degradation of I kappa B proteins allows nuclear translocation of NF-ka

166 ter (BTEE), which inhibit the degradation of I kappa B, or tricyclodecan-9-yl-xanthogenate-potassium

167 or interleukin-1 induces the degradation of I kappa B, the inhibitor of NF-kappa B, allowing nuclear

168 B in NIH 3T3 cells, including degradation of I kappa B-alpha, nuclear translocation of NF-kappa B sub

169 dent upon phosphorylation and degradation of I kappa B-alpha.

170 osphorylation and proteolytic degradation of I kappa B-alpha.

171 roteins, leading to the rapid degradation of I kappa B.

172 t that certain alterations in this domain of I kappa B alpha may have severe biological repercussions

173 ecifically binding the coiled-coil domain of I kappa B kinase gamma (IKK gamma) to inhibit IKK activi

174 he N-terminal and central ankyrin domains of I kappa B alpha.

175 Thus, noncoordinate expression of I kappa B kinases plays a role in determining the cell t

176 by expression of the superrepressor form of I kappa B alpha inhibited the loss of expression of both

177 ression of a mutated, superrepressor form of I kappa B alpha leads to a loss of beta-selected thymocy

178 or a degradation-resistant (Delta N) form of I kappa B alpha, an inhibitor of NF-kappa B, were challe

179 by the expression of a transdominant form of I kappa B-alpha, and downregulation of NF-kappa B by the

180 ion, a Drosophila gene encoding a homolog of I kappa B kinase (DmIkk beta) and Relish, a Rel-family t

181 nuclear p65 transactivation independently of I kappa B alpha degradation.

182 Inhibition of I kappa B alpha kinase by gossypin led to the suppressio

183 s demonstrated by HSR-mediated inhibition of I kappa B kinase activation and HSR-mediated induction o

184 ptide, an established selective inhibitor of I kappa B-kinase (IKK), blocked heregulin-mediated activ

185 together, our data implicate involvement of I kappa B kinase and MAPK signaling cascades in NIK-indu

186 for the differential degradation kinetics of I kappa B alpha and I kappa B beta.

187 int of control appears to be at the level of I kappa B alpha phosphorylation as demonstrated by HSR-m

188 ied by an increase in steady state levels of I kappa B alpha mRNA.

189 observed in sup- cells is not due to loss of I kappa B alpha.

190 ble phosphorylation site-deficient mutant of I kappa B alpha (I kappa B alpha M, S32/36A) was introdu

191 imulatory factors, whereas overexpression of I kappa B-alpha reduces Akt phosphorylation below basal

192 han that of E-selectin and overexpression of I kappa B-epsilon inhibited ICAM-1 and VCAM-1 promoter a

193 haracterized by decreased phosphorylation of I kappa B alpha and inhibition of NF-kappa B DNA binding

194 eatment leads to tyrosine phosphorylation of I kappa B alpha and NF kappa B transcriptional activatio

195 c-Src-dependent tyrosine phosphorylation of I kappa B alpha and subsequent activation of NF kappa B

196 activation through serine phosphorylation of I kappa B alpha by the IKK complex.

197 suggested not to require phosphorylation of I kappa B alpha for its targeted degradation by the prot

198 Tyrosine 42 phosphorylation of I kappa B alpha has been shown to mediate NF kappa B act

199 inhibited the stimulated phosphorylation of I kappa B alpha in cells (IC(50) = 4 microm) while faili

200 ted cells, EGCG inhibited phosphorylation of I kappa B alpha-GST (IC(50) > 18 microM) consistent with

201 B activation and tyrosine phosphorylation of I kappa B alpha.

202 s process is initiated by phosphorylation of I kappa B by the I kappa B kinase (IKK) complex, predomi

203 The phosphorylation of I kappa B kinase alpha and activation of NF kappa B medi

204 which is responsible for phosphorylation of I kappa B that retains NF-kappa B in the cytoplasm in an

205 unt for this peculiar degradation process of I kappa B alpha.

206 subsequent ubiquitination and proteolysis of I kappa B alpha, which then releases NF-kappa B to promo

207 is, LO expression decreased both the rate of I kappa B alpha turnover and the activities of IKK alpha

208 a cells, which leads to an increased rate of I kappa B phosphorylation and degradation.

209 ng the acidic C-terminal/PEST-like region of I kappa B alpha and the NF-kappa B p65 subunit N-termina

210 These data define a region of I kappa B alpha that may be required for optimal masking

211 resented in rASCs, whereas the regulation of I kappa B/nuclear factor-kappaB cascade were overreprese

212 Rel A.NF-kappa B1 induces the resynthesis of I kappa B alpha recapturing nuclear Rel A back into the

213 eta phosphorylates the N-terminal serines of I kappa B beta far less efficiently, thereby providing a

214 rter activity, the phosphorylation status of I kappa B alpha, and in vitro IKK activity.

215 kinase by gossypin led to the suppression of I kappa B alpha phosphorylation and degradation, p65 nuc

216 kinase activation, leading to suppression of I kappa B alpha phosphorylation and degradation.

217 light a novel function for the N-terminus of I kappa B alpha in the control of the subcellular locali

218 We demonstrate specific ubiquitination of I kappa B alpha by Ubc3 and Ubc4 in a phosphorylation an

219 phosphorylation dependent ubiquitination of I kappa B alpha.

220 and that this regulation occurs upstream of I kappa B degradation.

221 suggesting an increase in nuclear uptake of I kappa B alpha.

222 Degradation of I-kappa B alpha suggested involvement of NF-kappa B acti

223 Y cells expressing a super-repressor form of I-kappa B were completely resistant to doxorubicin killi

224 ppa B kinase (IKK) phospho-acceptor sites on I kappa B alpha, core components of the IKK signalsome,

225 demonstrate that amino acids 48 - 58 in p40/I kappa B alpha are essential to block the nuclear local

226 The phospho-I kappa B alpha generated by UV is only detectable by ex

227 the IKK catalytic subunits to phosphorylate I kappa B alpha and I kappa B beta.

228 ate trap for serine 32 and 36 phosphorylated I kappa B alpha.

229 over, AKT2 interacts with and phosphorylates I kappa B kinase alpha.

230 IKK phosphorylates I kappa B alpha on serines 32 and 36 signaling the inhib

231 serine/threonine kinase that phosphorylates I-kappa B alpha and I-kappa B beta and targets them for

232 d GST-I kappa B and GST-p65 phosphorylation, I kappa B degradation, and NF-kappa B DNA binding.

233 in a STAT6-dependent manner by 1) preventing I kappa B phosphorylation and thus NF-kappa B activation

234 activation of downstream pathways, primarily I kappa B/NF-kappa B, ERKs, and cJun/AP-1.

235 in the cytoplasm with its inhibitor protein I kappa B, whereas in cancer cells, NF-kappa B is in the

236 nt-negative mutant of the inhibitory protein I kappa B alpha results in down-regulation of constituti

237 gh the degradation of the inhibitory protein I kappa B alpha that sequesters NF-kappa B in the cytopl

238 somal degradation of its inhibitory protein, I kappa B alpha.

239 peroxisome proliferator-activated receptors, I kappa B kinase beta or nuclear factor kappa B do not a

240 Interestingly, we demonstrate Gli-regulated I-kappa-B kinase epsilon (IKBKE) and NF-kappaB activity

241 -Rel NLS, or for the nuclear export of c-Rel/I kappa B alpha complexes.

242 sfer of NF-kappaB inhibitor, super-repressor I kappa B alpha (Adv-SR-IkappaBalpha), blocked TNF-induc

243 FLASH antisense and super-repressor I kappa B alpha inhibited the action of TNF alpha indepe

244 pa B proteins are phosphorylated by the same I kappa B kinase (IKK) complex, and their ubiquitination

245 Thus Rac1 controls a second, I kappa B-independent, pathway to NF-kappa B activation

246 lving transduction of a superrepressive (sr) I kappa B alpha protein indicate that NF-kappaB/Rel prot

247 F-kappa B could be blocked by superrepressor I kappa B alpha without blocking apoptosis.

248 gradation of an associated inhibitor, termed I kappa B alpha.

249 tion contains two catalytic subunits, termed I kappa B kinase (IKK)-1 and IKK-2.

250 These results demonstrate that I kappa B alpha expression is regulated by NE at both tr

251 Our findings indicate that I kappa B alpha degradation mechanisms are dynamic durin

252 In this study, we show that I kappa B alpha M expression inhibits stress-induced NF-

253 The I kappa B kinase (IKK) complex, which is composed of the

254 The I kappa B kinase complex (IKK) mediates activation of th

255 Additionally, the I kappa B homolog Bcl-3, which functions to stimulate tr

256 r of the MAPK pathway and implicate both the I kappa B kinase and MAPK signaling cascades in NIK-indu

257 iated by phosphorylation of I kappa B by the I kappa B kinase (IKK) complex, predominantly by the IKK

258 dependent gene expression is mediated by the I kappa B kinase 2 (IKK2/IKK beta).

259 of the inhibitory I kappa B proteins by the I kappa B kinase complex (IKK).

260 initially by phosphorylation induced by the I kappa B kinase, which leads to ubiquitination and subs

261 One domain of Protac-1 contains the I kappa B alpha phosphopeptide that is recognized by the

262 h the adenovirus Ad5I kappa B expressing the I kappa B superrepressor to inhibit nuclear factor-kappa

263 uence and Rel-homology domains; however, the I kappa B-like domain is intact.

264 major transcript but it completely lacks the I kappa B-like domain-coding region, which has been repl

265 finding comparable to the expression of the I kappa B alpha dominant-negative mutant, which could be

266 pendently increase de novo expression of the I kappa B alpha gene, thereby providing another potentia

267 s expression of I kappa BNS, a member of the I kappa B family of NF-kappaB inhibitors whose expressio

268 Activation of the I kappa B kinase (IKK) complex by LPS induces phosphoryl

269 Here, we have explored an involvement of the I kappa B kinase (IKK) pathway, associated with nuclear

270 O is the essential regulatory subunit of the I kappa B kinase (IKK), encoded by an X-linked gene in m

271 Whereas Rac stimulates the activity of the I kappa B kinase IKK beta, Cdc42 and Rho activate NF kap

272 tor I kappa B-epsilon is a new member of the I kappa B protein family, but its functional role in reg

273 ng a cell-permeable peptide inhibitor of the I kappa B-kinase complex, a crucial component of signal

274 processing of p100 critically depends on the I kappa B kinase (IKK)alpha/IKK1 subunit of the IKK comp

275 C-beta)-deficient mice failed to recruit the I kappa B kinase (IKK) complex into lipid rafts, activat

276 ent with this finding, we also find that the I kappa B kinase (IKK) phospho-acceptor sites on I kappa

277 tly stimulating a key signal transducer, the I kappa B kinase (IKK).

278 sive bacteria have been shown to trigger the I kappa B/NF-kappa B transcriptional system and proinfla

279 These results correlated with the I kappa B alpha degradation needed for NF-kappa B activa

280 demonstrated that Hsp27 associates with the I kappa B kinase (IKK) complex and that this interaction

281 activates NF-kappaB via interaction with the I kappa B kinase (IKK) signalosome.

282 ssociation of SRC-3, but not SRC-1, with the I kappa B kinase (IKK).

283 We found that both TNF and LPS activated the I-kappa B kinase complex (IKK) in DPSCs to induce the ph

284 on of TAK1, which subsequently activates the I-kappa B kinase complex (IKK) and mitogen-activated pro

285 We recently identified a mutation in the I-kappa B kinase associated protein (IKBKAP) gene as the

286 sphorylate GST-I kappa B alpha and that this I kappa B alpha kinase activity is significantly reduced

287 broblasts maintained in the presence of TNF, I kappa B-alpha protein failed to return to base-line le

288 SUMO4 conjugates to I kappa B alpha and negatively regulates NF kappa B tran

289 ase (NIK)--IKK alpha/beta complex leading to I kappa B alpha phosphorylation and degradation, whereas

290 I kappa B-alpha phosphorylation, leading to I kappa B-alpha degradation and translocation of NF-kapp

291 lease from its cytosolic inhibitory sub-unit I kappa B alpha.

292 A and to a lesser extent with c-Rel, whereas I kappa B-alpha and I kappa B-beta associate with Rel A

293 e inhibitory I kappa B proteins, among which I kappa B alpha and I kappa B beta are the most abundant

294 Moreover, in melanoma cells in which I kappa B kinase activity was inhibited by sulindac to a

295 ited the TNF-induced interaction of Akt with I kappa B alpha kinase (IKK).

296 Blocking NF-kappa B with I kappa B super-repressor or AP-1 with dominant-negative

297 sequestered in the cytoplasm, complexed with I kappa B inhibitory proteins.

298 ic inhibition of the NF-kappa B pathway with I kappa B overexpression as well as with small interferi

299 diminished greatly in cells transfected with I kappa B alpha, the NF-kappa B inhibitor.

300 anscriptional activation in association with I-kappa B alpha degradation prior to loss of cell viabil