ライフサイエンスコーパス: IEF

1 IEF was performed in a mixture of four small and chemica

2 IEF/SDS-PAGE examination of irradiated tadpole brain hom

3 IEF/SDS/PAGE can precisely differentiate protein isoform

4 DS PAGE)-LA ICP MS, and, in duplicate, by 2D IEF-PAGE.

5 compared to one-dimensional SPLC-MS, the 2D IEF-SPLC-MS platform resulted in a 5-6x increase in the

6 Depending on the sensor matrix, they allowed IEF within a pH range of roughly 5.5-10.5 with good sens

7 ally useful for evaluating the quality of an IEF or NEPHGE tube gel before using it for the second di

8 Early enteral feeding with an IEF has been associated with improved outcome in trauma

9 Early enteral feeding with an IEF was not beneficial and should not be used in a routi

10 nel passivation, electroosmosis control, and IEF linearity control.

11 Continuum solvation methods (SMD and IEF-PCM) and the MPWB1K functional are used.

12 lumes of interest from the IEF dimension, as IEF was 'parked' during each CE analysis and refocused p

13 inducible phosphoprotein STI1 (also known as IEF SSP 3521 or p60).

14 heterogeneous separation techniques, such as IEF, capillary electrophoresis, and liquid chromatograph

15 included B3LYP, MP2, and CBS-QB3, as well as IEF-PCM to approximate implicit water solvation.

16 focusing (IEF) and in situ antibody blotting IEF was employed to monitor dark (nonfluorescent) and br

17 These findings were confirmed by IEF coupled with size exclusion chromatography on Supero

18 tablish the tunable pH gradients required by IEF and precisely control the transport and handling of

19 Of the 10 isoforms of eIF4E-BP1 resolved by IEF/SDS/PAGE, at least seven are labelled with [32P] and

20 borns from expensive confirmatory testing by IEF, without missing any of the SCD newborns in the stud

21 Analysis of the treatments was undertaken by IEF/SDS-PAGE, comparison of protein spot abundances and

22 precolumn labeling and cannot be used for CA-IEF, at least without more detailed study of the dye-pro

23 d comparable resolution to prominent on-chip IEF techniques.

24 g experimental time compared to conventional IEF IGP gel strip approaches.

25 The first electrophoretic dimension (IEF OFFGEL) showed that dissolved proteins in surface se

26 reversed-phase HPLC in the second dimension (IEF-NP RP HPLC).

27 by 1D isoelectric focusing electrophoresis (IEF)-laser ablation (LA) inductively coupled plasma mass

28 sulfate polyacrylamide gel electrophoresis (IEF/SDS-PAGE) and fluorescence measurements.

29 ct") via an isoelectric focusing experiment (IEF).

30 n example, outlet fractions from cascaded FF-IEF were analyzed by sodium dodecyl sulfate polyacrylami

31 demonstrate the compatibility of cascaded FF-IEF with common bioanalytical tools.

32 abricated free-flow isoelectric focusing (FF-IEF) device.

33 we present a microfluidic free flow IEF (FF-IEF) device for continuous protein separation into 24 fr

34 demonstrate improved separation with the FF-IEF system over traditional 2D gel electrophoresis.

35 nal IEF, we present a microfluidic free flow IEF (FF-IEF) device for continuous protein separation in

36 c proteomic analysis of isoelectric focused (IEF) cerebrospinal fluid (CSF) immunoglobulin G (IgG) wa

37 um techniques such as iso-electric focusing (IEF) have become instrumental within analytical chemistr

38 y couples liquid-phase isoelectric focusing (IEF) and free solution capillary electrophoresis (CE).

39 -resolved microfluidic isoelectric focusing (IEF) and in situ antibody blotting IEF was employed to m

40 of T. pallidum by the isoelectric focusing (IEF) and nonequilibrating pH gel electrophoresis (NEPHGE

41 s based on concomitant isoelectric focusing (IEF) detection of B gamma2- and gamma3-CN fragments afte

42 of ultrafiltration and isoelectric focusing (IEF) electrophoresis steps.

43 m whole sporozoites by isoelectric focusing (IEF) following observations that the approximately 1,300

44 labeled proteins using isoelectric focusing (IEF) have been made.

45 ta (Abeta) peptides by isoelectric focusing (IEF) in 75 nL microchannels combined with their analysis

46 the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic chip.

47 tigation of the use of isoelectric focusing (IEF) in microfluidic devices, pH gradients were electroc

48 to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentratio

49 Isoelectric focusing (IEF) is the first step for two-dimensional (2D) gel elec

50 we applied a capillary isoelectric focusing (IEF) method to determine the pattern of FOXO3 posttransl

51 After isoelectric focusing (IEF) of a methylated tryptic digest of a mixture of alph

52 Isoelectric focusing (IEF) of Cx43 in HuH-7 cells indicated that the pIs of th

53 ion polymorphism), and isoelectric focusing (IEF) of water-soluble proteins of fish fillet were appli

54 ter by two-dimensional isoelectric focusing (IEF) OFFGEL-lab-on-chip (LOC) electrophoresis after tang

55 After separation by isoelectric focusing (IEF) or non-equilibrium pH gradient electrophoresis (NEP

56 recognized by altered isoelectric focusing (IEF) patterns of serum transferrin (Tf).

57 were then subjected to isoelectric focusing (IEF) separation by a digital ProteomeChip (dPC), followe

58 eta phosphorylation by isoelectric focusing (IEF) shows that LAP is present in multiple forms, each w

59 , and bla(OXA), and in isoelectric focusing (IEF) tests, all isolates demonstrated at least one beta-

60 platform that includes isoelectric focusing (IEF) through immobilized pH gradient and superficially p

61 Sub-zero isoelectric focusing (IEF) was employed to investigate the extent of hybrid fo

62 The new methods couple isoelectric focusing (IEF) with high ionic strength electrophoretic separation

63 , combining non-native isoelectric focusing (IEF) with sodium dodecyl sulfate (SDS) gel electrophores

64 By combining isoelectric focusing (IEF) with subsequent gel electrophoresis, two-dimensiona

65 By isoelectric focusing (IEF), 7 of the 23 isolates contained a beta-lactamase wi

66 Compared to isoelectric focusing (IEF), the CZE method provides more quantitative results

67 kers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptamer/antibody-based metho

68 eneity was analyzed by isoelectric focusing (IEF).

69 mpared to conventional isoelectric focusing (IEF).

70 methods were used for isoelectric focusing (IEF).

71 l incompatibility with isoelectric focusing (IEF).

72 ses by two-dimensional isoelectric focusing (IEF)/SDS-PAGE not only revealed different alpha and beta

73 a, acid urea PAGEs and isoelectric focusing (IEF)] as the first-dimension (1-DE).

74 f protein labeling with noncovalent dyes for IEF is investigated.

75 Both analytical and computational models for IEF suggest device performance will be improved by utili

76 desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requi

77 al feeding with an immune-enhancing formula (IEF) decreases morbidity, mortality, and length of hospi

78 in depletion, lectin affinity fractionation, IEF separation, and LC-MS analysis has been applied to d

79 tially sampling protein analytes eluted from IEF, focused proteins are electrokinetically transferred

80 as do several tryptic peptides produced from IEF SSP 3521 and chick p60).

81 FS-IEF-derived CRCL-pulsed DCs are a promising anticancer v

82 -solution isoelectric focusing technique (FS-IEF) to obtain chaperone-rich cell lysate (CRCL) fractio

83 genes (ISGs) and immune effector functions (IEFs).

84 ased technology (MGT), which combines in-gel IEF-LC-MS/MS and SDS-PAGE-LC-MS/MS strategies.

85 and quantitatively to conventional slab gel IEF.

86 urification (bulk soil --> crude colloid --> IEF colloid) and coincided with the trend of Pu concentr

87 ine WB milk/dairy products and comigrates in IEF with B gamma2-CN.

88 lectin column (M-LAC) and the pI profiles in IEF separation.

89 73x) of species were achieved in the initial IEF dimension.

90 ilized pH gradient isoelectric focusing (IPG-IEF) for the analysis of these apolipoproteins isolated

91 Single and double isoelectrofocusing (IEF) of porcine sperm extracts generated fractions with

92 entration of the eluate in the microchannel, IEF-micropillar-MALDI-MS is demonstrated to be a suitabl

93 resolution (0.1 pH unit) and rapid (<20 min) IEF.

94 ation formalism polarizable continuum model (IEF-PCM) at the HF/6-31+G(d) level.

95 The development of this novel IEF method for the simultaneous quantification of differ

96 ndard proteins demonstrates compatibility of IEF-SPLC processing and high resolving-power MS analysis

97 s also demonstrate that by the generation of IEF-SPLC protein libraries by fraction collection, the p

98 ognized by 3E2 in 2-D Western immunoblots of IEF-isolated CSL.

99 the array, thereby increasing the number of IEF fractions further analyzed in the size-based separat

100 nsional separation, including rehydration of IEF strip and fluorescence detection was completed in 2.

101 This method couples several stages of IEF in series by first focusing proteins in a straight c

102 Three stages of IEF were completed in less than 25 min at electric field

103 system described here facilitates the use of IEF markers for internal calibration of pI.

104 On IEF gels, only 4 to 20 bands were observed in the anti-S

105 ies include IgG2 dominance, limited bands on IEF gels, supporting an oligoclonal response, and use of

106 Our IEF results were consistent with theoretical pIs of meth

107 leavage at cysteine 245, focused on PhastGel IEF near pH 3.4, indicating the presence of three phosph

108 This work shows that a preparative IEF method using immobilized pH gradients can be optimiz

109 A variety of preparative IEF methods have been developed over the years but have

110 Therefore, preparative IEF methods are needed to fractionate charge variants fo

111 Our facile dynamic and probed IEF assays should find widespread use in analytical scre

112 o the transformation-sensitive human protein IEF SSP 3521 and mouse extendin.

113 In addition to purification, IEF preconcentration provides at least a 10-fold increas

114 toward Phe-pNA, together with other results (IEF) suggest the presence of more than one aminopeptidas

115 Most showed IEF profiles consistent with production of both a TEM an

116 to be applicable by using focusing of small IEF markers as a demonstration.

117 en fluorescent protein from the second-stage IEF fractionation were further separated in a third stag

118 ere is more convenient than room-temperature IEF techniques, which require Hb mixtures in the deoxy s

119 with these symptoms and similar abnormal Tf IEF patterns were analyzed by metabolic labeling of fibr

120 It is demonstrated that IEF-NP RP HPLC provides a viable alternative to the 2-D

121 The IEF group received significantly more protein, carbohydr

122 The IEF profiles of the remaining 16 isolates showed a varie

123 g-cc-pVTZ and 6-31+G(d,p) basis sets and the IEF-polarizable continuum model (PCM) solvation model.

124 M06/def2-TZVPD levels of theory applying the IEF-PCM water and MeCN solvation models, all of which su

125 To define the IEF axis along a "lane" at the top of the chamber, we us

126 YP/6-31+G(d,p) level of theory employing the IEF-PCM CHCl3 solvation model were also performed.

127 loric intake was 61% and 22% of goal for the IEF and CNTL groups, respectively.

128 used to repeatedly inject effluent from the IEF dimension into an ampholyte-based CE separation.

129 yzing all fluid volumes of interest from the IEF dimension, as IEF was 'parked' during each CE analys

130 ed the presence of HS functionalities in the IEF extract.

131 human erythroleukemia cell line lysate, the IEF-NP RP HPLC produced improved resolution of low mass

132 ry (ESI FTICR-MS) spectral comparison of the IEF extract and a siderophore standard (desferrioxamine;

133 t-stable proteins in the anodic range of the IEF gels.

134 f 20 microm/s during characterization of the IEF step.

135 Mobilization drove focused proteins off the IEF lane and into a region for protein gel electrophores

136 y chemical mobilization perpendicular to the IEF axis.

137 ed proteins increased significantly when the IEF fractionation step was included as part of the platf

138 the solubilisation buffer combined with the IEF approach.

139 d,p), and B97D/6-311+G(d,p) methods with the IEF-PCM solvation model for chloroform and ethanol.

140 Through IEF, each population was observed to exhibit distinct is

141 r underwent resection and were randomized to IEF via jejunostomy tube or control (CNTL).

142 of a fluid volume spanning 15% of the total IEF channel length was completed in less than 5 min.

143 dress some of the limitations of traditional IEF, we present a microfluidic free flow IEF (FF-IEF) de

144 Transverse IEF in pressure-driven flow is demonstrated using bovine

145 te the potential of "microfluidic transverse IEF" for use in continuous concentration and separation

146 g states in the solvent phase (DF/def2-TZVP; IEF-PCM and/or SMD) to investigate how well the experime

147 Using IEF-NP RP HPLC, approximately 700 bands were resolved in

148 y of eIF4E-BP1 isoforms was determined using IEF/SDS/PAGE/immunoblotting of unfractionated cell lysat

149 There was one bowel necrosis associated with IEF requiring reoperation.

150 and band-broadening behavior consistent with IEF and CE, respectively.

151 The sub-zero IEF technique discussed here is more convenient than roo