ライフサイエンスコーパス: IGFBP-2

1 nsulin-like growth factor binding protein 2 (IGFBP-2).

2 hybridization to determine the expression of IGFBP-2.

3 yze the structure of the IGF-binding site on IGFBP-2.

4 g domain is located within the C terminus of IGFBP-2.

5 ges were rescued following administration of IGFBP-2.

6 f the CWCV motif (residues 247-250) in human IGFBP-2.

7 tion and dissociation rates than full-length IGFBP-2.

8 hyroglobulin-RGD region of the C terminus of IGFBP-2.

9 1.16; 95% CI: 1.08-1.24; P(trend) < 0.001), IGFBP-2 (1.18; 1.06-1.31; P(trend) < 0.01) and IGFBP-3 (

10 al domain had no further effect on affinity (IGFBP-2(1-190) EC50 = 9.2 nM).

11 In kinetic assays, IGFBP-2(1-248) and IGFBP-2(1-190) exhibited more rapid association and diss

12 FBP-2(1-248) and IGFBP-2(249-289) as well as IGFBP-2(1-190) were expressed as glutathione S-transfera

13 binding affinity (IGFBP-2 EC50 = 0.35 nM and IGFBP-2(1-248) = 7 nM).

14 In kinetic assays, IGFBP-2(1-248) and IGFBP-2(1-190) exhibited more rapid a

15 (BNPS-skatole) and the BNPS-skatole products IGFBP-2(1-248) and IGFBP-2(249-289) as well as IGFBP-2(1

16 the BNPS-skatole products IGFBP-2(1-248) and IGFBP-2(249-289) as well as IGFBP-2(1-190) were expresse

17 studies revealed that normal RPE can produce IGFBP-2, -3, -4, -5, and -6 but not IGFBP-1.

18 ogressive increases in message abundance for IGFBP-2, -3, -4, and -6.

19 Likewise, although IGFBP-2, -3, and -5 mRNA levels fell, membrane-associate

20 IGFBP-2, -3, and -5 were effective inhibitors of both li

21 ncreased message abundance and production of IGFBP-2, -4, -5, and -6, but not IGFBP-3.

22 roliferation with coaddition of 20 or 100 nM IGFBP-2 (50 or 80% inhibition, respectively).

23 and shows high sequence identity with human IGFBP-2 (52%).

24 IGFBP-2, a regulator of insulin-like growth factor actio

25 We also tested the ability of IGFBP-2, a related binding protein which has an arginine

26 An anti-IGFBP-4Ab, but not an anti-IGFBP-2 Ab or normal rabbit serum, shifted the [125I] IG

27 on MCF-7 breast cancer cells, we found that IGFBP-2 also regulates PTEN.

28 Among the other IGFBPs, the N-domains of IGFBP-2 and -3 also had strong transactivation activity,

29 at are critical for transactivation and that IGFBP-2 and -3 also have strong transactivation activity

30 IGFBP-2 and -3, the major binding proteins identified in

31 like growth factor-binding proteins (IGFBP), IGFBP-2 and -4, and that these IGFBPs modulate IGF-I-sti

32 d porcine vitreous contain two major IGFBPs, IGFBP-2 and an approximately 29-kDa fragment of IGFBP-3.

33 We synthesized a HBD peptide specific for IGFBP-2 and demonstrated in vitro that it rescued the mi

34 novel mechanism for coordinate regulation of IGFBP-2 and IGF-I signaling functions that lead to stimu

35 IGFBP-binding domain on IGF-1 in binding to IGFBP-2 and IGFBP-3 and support involvement of the IGF-1

36 In BIAcore analysis, IGFBP-2 and IGFBP-3 bound only to the N(epsilon)(Lys65/6

37 igh affinity ( approximately 0.1-0.2 nM) for IGFBP-2 and IGFBP-3 in solid-phase-binding assays.

38 and cross-sectional insulin, IGF-I, IGF-II, IGFBP-2 and IGFBP-3 in the Boyd Orr cohort (n = 182 men,

39 Lower preoperative IGFBP-2 and IGFBP-3 levels and biopsy Gleason score were

40 ns of IGF-1 involved in the interaction with IGFBP-2 and IGFBP-3, we employed IGF-1 selectively bioti

41 ies were observed in ligand blot analysis of IGFBP-2 and IGFBP-3.

42 y of Gly 1 and Lys 27 when IGF-1 is bound to IGFBP-2 and IGFBP-3.

43 he effects of individual IGFBPs were tested, IGFBP-2 and IGFBP-4 were found to inhibit IGF-I-stimulat

44 SF levels of IGFBP-2 and IL-6 did not differ by treatment group.

45 gene, IIp45, whose protein product binds to IGFBP-2 and inhibits glioma cell invasion.

46 The link between IGFBP-2 and PTEN was of particular interest because elev

47 We investigated the role of IGFBP-2 and receptor protein tyrosine phosphatase beta (

48 spermidine and spermine elevated endogenous IGFBP-2 and SSAT mRNA abundance, whereas, putrescine sti

49 identity of the approximately 35-kDa band as IGFBP-2 and the approximately 29-kDa band as a fragment

50 rtilage, and ERT increases the production of IGFBP-2 and the synthesis of PGs by chondrocytes from su

51 ally important roles for circulating IGF-II, IGFBP-2, and IGFBP-3 in PSA-detected prostate cancer, in

52 d measurements of blood serum IGF-I, IGF-II, IGFBP-2, and IGFBP-3 obtained at recruitment.

53 Plasma levels of IGF-I, IGFBP-2, and IGFBP-3 were measured preoperatively in 120

54 an and porcine vitreous, plasma, recombinant IGFBP-2, and IGFBP-3 were separated by gel electrophores

55 ions of native IGF-I, native IGFBP-1, native IGFBP-2, and their respective analogues/mutants were app

56 hemistry experiments using a polyclonal anti-IGFBP-2 antibody were performed to confirm IGFBP-2 prote

57 Levels of IGFBP-2 are elevated frequently in human tumors: its abi

58 rther support the development of full-length IGFBP-2 as a cancer therapeutic.

59 found that IGF-II enhanced by over threefold IGFBP-2 binding to extracellular matrix produced by huma

60 BP-2 with a specific antibody, or preventing IGFBP-2 binding to integrins, restored the induction of

61 These cells predominantly produce IGFBP-2: blocking IGFBP-2 with a specific antibody, or p

62 IGFBP-2 bound RPTPbeta, which led to its dimerization an

63 cells stably transfected with the zebrafish IGFBP-2 cDNA secreted a 31-kDa protein, which bound to I

64 cellular matrix-rich environment, the IGF-II/IGFBP-2 complex was as effective as IGF-II alone in stim

65 Immunohistochemistry studies for IGFBP-2 confirmed the expression pattern found by in sit

66 Plasma IGFBP-2 content was significantly reduced in prenatal T-

67 ratio of IGF-I to IGFBP-3 increased, whereas IGFBP-2 decreased throughout pregnancy.

68 20-fold decrease in IGF-1 binding affinity (IGFBP-2 EC50 = 0.35 nM and IGFBP-2(1-248) = 7 nM).

69 IGFBP-2 enhanced IGF-I-stimulated VSMC migration and pro

70 ively, these studies establish that PTEN and IGFBP-2 expression are inversely correlated in human bra

71 his study was conducted to determine whether IGFBP-2 expression in human RPE cells from the macula an

72 is a topographical and age-related change in IGFBP-2 expression in RPE cells from human donor eyes.

73 er investigate this link, we determined that IGFBP-2 expression is negatively regulated by PTEN and p

74 To further test their contributions to IGFBP-2 function, the single tryptophan in human IGFBP-2

75 As that encode transcription factors for the IGFBP-2 gene also were induced in some ST tissues.

76 lated IGFBP-2 mRNA abundance and transfected IGFBP-2 gene promoter activity in human (Hec-1-A) uterin

77 nsulin-like growth factor binding protein-2 (IGFBP-2) gene.

78 is unique to IGFBP-1 as the closely related IGFBP-2 had no effect, a finding consistent with its ina

79 We conclude that the HBD peptide of IGFBP-2 has anabolic activity by activating IGF-I/Akt an

80 t IGF-I and its binding proteins IGFBP-1 and IGFBP-2 have potentially beneficial effects on diabetes

81 nsulin-like growth factor binding protein 2 (IGFBP-2); histone H2B.1; basement membrane heparan sulfa

82 complexes with several IGF-binding proteins (IGFBP-2, IGFBP-3, and IGFBP-5).

83 IGF-I and several forms of IGFBPs, including IGFBP-2, IGFBP-4, and IGFBP-5.

84 In sum, the IGF axis, IGFBP-2 in particular, may be implicated in the pathogen

85 broblasts, implicating a functional role for IGFBP-2 in PTEN signaling.

86 Increased IGFBP-2 in these cultures was associated with a 1.41-fol

87 h increased levels of IGF-binding protein-2 (IGFBP-2) in the uterus and ovary, and a reduction in IGF

88 In the current study, we postulated that IGFBP-2 increased bone mass partly through the activity

89 These results indicate that zebrafish IGFBP-2 is a negative growth regulator acting downstream

90 For example, IGFBP-2 is expressed in the inner circular layer shortly

91 data support a growing body of evidence that IGFBP-2 is not just a transport protein but rather that

92 nsulin-like growth factor-binding protein 2 (IGFBP-2) is a member of a family of six highly conserved

93 nsulin-like growth factor binding protein 2 (IGFBP-2) is frequently overexpressed in the highly invas

94 IGFBP-2 knockdown led to decreased PTEN tyrosine phospho

95 calized prostate cancer, preoperative plasma IGFBP-2 levels are inversely associated with biologicall

96 ain and prostate cancers and implicate serum IGFBP-2 levels as a potential serum biomarker of PTEN st

97 f particular interest because elevated serum IGFBP-2 levels have been reported in patients with prost

98 Elevation of plasma IGFBP-2 levels in prostate cancer patients apparently is

99 was fivefold lower among women with baseline IGFBP-2 levels in the top versus bottom quintile (odds r

100 IGFBP-2 levels rise during rapid neonatal growth and at

101 IGFBP-2 levels were elevated in prostate cancer patients

102 prostatectomy patients, preoperative plasma IGFBP-2 levels were lower in patients with advanced dise

103 Serum IGF-I, IGFBP-1, and IGFBP-2 levels were measured by radioimmunoassay, and IG

104 relative to L/L-TRAMP mice (P < 0.001), but IGFBP-2 levels were not different.

105 In contrast, higher IGFBP-2 levels were related to a substantially lower ris

106 Thus, IGFBP-2 may facilitate the targeting of IGFs, and in par

107 Analysis of aortas obtained from IGFBP-2(-/-) mice showed that RPTPbeta was activated, an

108 NA abundance, whereas, putrescine stimulated IGFBP-2 mRNA abundance and transfected IGFBP-2 gene prom

109 IGFBP-2 mRNA expression was detected in the ganglion cel

110 In 16 of 17 eyes, IGFBP-2 mRNA expression was detected in the RPE.

111 The total numbers of RPE cells and IGFBP-2 mRNA expression-positive RPE cells were counted

112 In adult zebrafish, IGFBP-2 mRNA levels were greatly reduced by growth hormo

113 in insulin growth factor-binding protein 2 (IGFBP-2) mRNA.

114 insulinlike growth factor-binding protein 2 (IGFBP-2), nerve growth factor (b-NGF), platelet-derived

115 xia did not affect protein or mRNA levels of IGFBP-2 or -4.

116 r, these results suggest that, once bound to IGFBP-2 or IGFBP-3, the biotin moieties of N(alpha)(Gly1

117 pression of PAPP-A led to degradation of the IGFBP-2 produced by C2C12 myoblasts and increased free I

118 unt of free IGFs via specific degradation of IGFBP-2 produced by myoblasts.

119 Compared with untreated controls, IGFBP-2 production was significantly increased (P < 0.00

120 chromatin, they abolish menin binding to the IGFBP-2 promoter in vivo.

121 i-IGFBP-2 antibody were performed to confirm IGFBP-2 protein expression.

122 domain on IGF-1 contacts the distal third of IGFBP-2, providing evidence that the IGF-1-binding domai

123 nsulin-like growth factor-binding protein-2 (IGFBP-2) resulted in an increase in synthesis in both th

124 ypsin-digested abG(1)IGF-1.recombinant human IGFBP-2 (rhIGFBP-2) complex indicated photoincorporation

125 Recombinant human IGFBP-2 significantly attenuated IGF-1-stimulated MCF-7

126 zebrafish and mammalian cells, the zebrafish IGFBP-2 significantly inhibited IGF-I-stimulated cell pr

127 roximately 50-kD complex in association with IGFBP-2; this complex can cross the capillary barrier an

128 n 45 (IIp45), whose protein product bound to IGFBP-2 through the thyroglobulin-RGD region of the C te

129 rn blot analyses revealed that the zebrafish IGFBP-2 transcript is a 1.8-kb band expressed in many em

130 P-2 function, the single tryptophan in human IGFBP-2, Trp-248, was selectively cleaved with 2-(2'nitr

131 In Boyd Orr, a one SD increase in IGFBP-2 was associated with 2.6% slower get-up and go ti

132 Up-regulation of IGFBP-2 was confirmed at the protein level by Western bl

133 IGFBP-2 was present in all samples, IGFBP-4 in sporadic

134 Of the IGFBPs, an increase in IGFBP-2 was unique to these patients and was not found i

135 ontrast, serum levels and mRNA expression of IGFBP-2 were increased 1.6-fold (P = .003) and twofold (

136 GD-IGFBP-1, des(1-3)IGF-I/IGFBP-1, and IGF-I/IGFBP-2 were ineffective.

137 oses of IGF-II due to induction of PTEN, but IGFBP-2, when free from IGF-II can suppress PTEN.

138 of insulin growth factor-binding protein-2 (IGFBP-2), which is secreted thereby promoting angiogenes

139 ells predominantly produce IGFBP-2: blocking IGFBP-2 with a specific antibody, or preventing IGFBP-2