ライフサイエンスコーパス: IL-12 p40

1 IL-12 p40 expression occurred only in monocytes/macropha

2 IL-12 p40 mRNA and IL-12 p70 protein were also found to

3 IL-12 p40 mRNA was up-regulated in a dose-dependent mann

4 IL-12 p40 subunit mRNA by using the patient's PBMCs afte

5 IL-12 p40, IFN-gamma, TNF, IL-1alpha, and IL-6 were show

6 IL-12 p40-deficient mice (12KO) were resistant to EAU in

7 , interleukin-1beta (IL-1beta), IL-6, IL-10, IL-12 p40, alpha interferon (IFN-alpha), and IFN-gamma,

8 gingivalis LPS-induced production of IL-10, IL-12 p40, and IL-12 p70 by human monocytes was investig

9 ressed intragraft interleukin (IL)-4, IL-10, IL-12 p40, and IL-15 mRNA expression.

10 protein concentrations for TNF-alpha, IL-10, IL-12 p40, IFN-gamma, and IL-4.

11 ntracellular cytokine staining (IL-5, IL-10, IL-12 p40, TNF-alpha, and IFN-gamma) and cytometric bead

12 ioimmunoassays, interleukin-4 (IL-4), IL-10, IL-12 p40, tumor necrosis factor alpha (TNF alpha), inte

13 serum, significant decreases in IL-6, IL-10, IL-12 p40, tumor necrosis factor-alpha, IFN-gamma, and I

14 S-induced production of TNF-alpha, IL-1beta, IL-12 p40, and nitric oxide.

15 cytes that expressed the Th1 cytokines IL-2, IL-12 p40, and IFN-gamma mRNA was markedly diminished in

16 Expression of cytokines IL-2, IL-4, IL-5, IL-12 p40, IL-13, and interferon gamma is not affected b

17 crease expression of IL-1beta, IL-1Ra, IL-8, IL-12 p40, RANTES, macrophage inflammatory protein-1alph

18 bacterial products synergistically activate IL-12 p40 gene expression are less clear.

19 ependent role for EKLF binding in activating IL-12 p40 transcription in resting RAW264.7 cells, where

20 These data suggest that the aggregate IL-12 p40 and p70 response to endotoxemia in vivo is IFN

21 The induction of TNF-alpha, IL-12 p40, and MIP-2 (CXCL2) expression by S. aureus- an

22 by reverse transcription-PCR amplification, IL-12 p40 mRNA was constitutively expressed in LC RNA ex

23 is comprised of an IL-12 p35 subunit and an IL-12 p40-related protein subunit, EBV-induced gene 3 (E

24 minantly involved in production of IL-10 and IL-12 p40 by MPL-stimulated monocytes.

25 owever, reduced expression of both IL-10 and IL-12 p40 mRNA were found in Mac-3+ cells from male mice

26 e eliminated the reduction of both IL-10 and IL-12 p40 mRNA, suggesting that the Th2 inducer phenotyp

27 n, because mice deficient for both IL-10 and IL-12 p40 show no intestinal pathology following H. hepa

28 ated with high growth of Mtb, high IL-10 and IL-12 p40, nearly undetectable IL-12 p70 levels, and the

29 pha, IL-1 beta, IL-4, IL-6, IL-8, IL-10, and IL-12 p40 and histopathology.

30 inducible nitric-oxide synthase, IP-10, and IL-12 p40 mRNA, whereas no increase or relatively small

31 , GM-CSF, IL-1 alpha, IL-2, IL-6, IL-10, and IL-12 p40.

32 arriers had higher serum levels of IL-12 and IL-12 p40 in comparison with controls (P < 0.01), sugges

33 -gamma), IL-4, IL-5, IL-6, IL-10, IL-13, and IL-12 p40 compared to those for wt rRSV.

34 ors, including TNF, CCL5, CXCL10, IL-18, and IL-12 p40, and identified 140 drugs targeting 16 of them

35 induction of I-A, CD80, CD86, IL-1beta, and IL-12 p40 while inhibiting the expression of IL-10, sugg

36 ethyl-4-phenylpyridinium(+)-, IL-1beta-, and IL-12 p40(2)-induced microglial expression of iNOS.

37 f LPS-induced TNF-alpha, IL-1beta, IL-6, and IL-12 p40 mRNA.

38 ntial effects on production of TNF-alpha and IL-12 p40 by human monocytes, whereas MPL-induced activa

39 ses of rgpTNF-alpha suppressed TNF-alpha and IL-12 p40 mRNA.

40 stimulated spleen reduced both TNF-alpha and IL-12 p40 production, but 33D1+ dendritic cell removal o

41 The mRNA levels for TNF-alpha and IL-12 p40 were measured using real-time PCR.

42 intensified the mRNA levels of TNF-alpha and IL-12 p40.

43 on, including the expression of CD8alpha and IL-12 p40 as well as major histocompatability complex cl

44 comparable expansions of dendritic cells and IL-12 p40 productive capacity in both infected and uninf

45 iderably greater expression of IFN-gamma and IL-12 p40 mRNAs in the LN draining a locally controlled

46 oclonal antibody restored LPMC IFN-gamma and IL-12 p40 secretion.

47 s in response to IRBP: reduced IFN-gamma and IL-12 p40, but unchanged levels of TNF-alpha, IL-4, IL-5

48 ponses in anti-CD40-treated IFN-gamma-/- and IL-12 p40-/- C57BL/6 mice were unaffected, although anti

49 ospot assays indicated that IFN-gamma-/- and IL-12 p40-/- gene knockout mice developed CTL responses

50 -gamma receptor knockout (IFN-gammaR-/-) and IL-12 p40 knockout (IL-12-/-) mice.

51 duction of immunoglobulin A (IgA), IgG1, and IL-12 p40 from colon fragment cultures.

52 omoter activity in macrophage cell lines and IL-12 p40 mRNA is reduced in MKK3-deficient mice.

53 Mitigated induction of COX-2, IL-12 p35, and IL-12 p40 gene expression by CD11b/CD18-deficient macrop

54 r optimal induction of COX-2, IL-12 p35, and IL-12 p40 genes by low concentrations of LPS or by all c

55 es were virtually identical in wild-type and IL-12 p40(-/-) mice.

56 l IFN-gamma production in both wild-type and IL-12 p40-/- mice.

57 constitutively express the IL-12 antagonist, IL-12 p40.

58 ndeed, neutralizing endogenous p40 with anti-IL-12 p40 mAb reduced Th1 development in p35-/- allograf

59 of infected colitic IL-10 KO mice with anti-IL-12 p40 resulted in markedly reduced intestinal inflam

60 s were diminished by MAb treatment with anti-IL-12 p40.

61 nal, since inhibition of this pathway blocks IL-12 p40 promoter activity in macrophage cell lines and

62 Both IL-12 p40 and IFN-gamma levels in CGRP-treated cultures

63 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p

64 ng with IFN-gamma, through increases in both IL-12 p40 and p35 mRNA levels, caused a significant incr

65 he mechanism(s) of IL-10 suppression of both IL-12 p40 and IL-12 p35 genes is primarily seen at the t

66 Farnesol inhibited production of both IL-12 p40 and p70 from IFN-gamma/LPS-stimulated macropha

67 udies, we observed increased amounts of both IL-12 p40 monomer and homodimer in the serum of C57BL/6

68 iple apoptotic pathways, dependent upon both IL-12 p40 and FasL, that may play a role in the lethal p

69 nally, injection of the pIL-12o- into BALB/c IL-12 p40-/- mice also resulted in a biphasic pattern of

70 (IFN-gamma 0/0) produced as much circulating IL-12 p40 and IL-12 p70 as did IFN-gamma +/+ mice follow

71 In contrast, total circulating IL-12 p40 was reduced by only 30-50%.

72 Studies comparing IL-12 p40- and p35-deficient RAG(-/-) mice indicated tha

73 In contrast, IL-12 p40 mRNA expression by cells from animals with lat

74 In contrast, IL-12 p40 mRNA was only detectable in the brain of mice

75 scription factor in macrophages, controlling IL-12 p40 production induced by bacterial products and t

76 IL-15, TNF-alpha, IL-12 p70, IL-17A, GM-CSF, IL-12 p40, IL-10, IL-7, IL-1alpha, and IL-5) subject-to-

77 e 1, resulted in production of the cytokines IL-12 p40 and TNF-alpha by a mechanism independent of re

78 the set of p38 MAPK-dependent genes in DCs (IL-12 p40 and cellular inhibitor of apoptosis protein-2)

79 suggest a novel role for epithelial-derived IL-12 p40 in modifying the level of airway inflammation

80 In contrast, no detectable IL-12 p40 protein was secreted in CD40-stimulated B cell

81 minant-negative construct of PU.1 diminishes IL-12 p40 promoter activity and endogenous IL-12 p40 pro

82 e to overt increases in production of either IL-12 p40 or IL-10.

83 onfirm that this IFN primes DCs for elevated IL-12 p40 and p70 secretion.

84 responded to RANKL stimulation with elevated IL-12 p40 mRNA expression, Peyer's patch DC instead pref

85 ates LPS- and IFN-gamma-activated endogenous IL-12 p40 mRNA expression.

86 s IL-12 p40 promoter activity and endogenous IL-12 p40 protein secretion.

87 Murine endogenous IL-12 p40 mRNA was consistently induced by overexpressed

88 y transfectable and regulates the endogenous IL-12 p40 gene in response to IFN-gamma or LPS similarly

89 molecular events occurring at the endogenous IL-12 p40 locus in lipopolysaccharide-stimulated periton

90 CBP alone led to induction of the endogenous IL-12 p40 mRNA in the absence of IFN-gamma and LPS.

91 ed LC maturing into dendritic cells, express IL-12 p40 mRNA, as well as p40 and functional p70 protei

92 -specific CD4+ T cells transduced to express IL-12 p40 into mice immunized to develop experimental au

93 Consistent with this idea, fewer IL-12 p40-secreting Mac-3+ cells were found in male mice

94 a 0/0 mice generated two- to threefold fewer IL-12 p40-secreting cells following in vitro or in vivo

95 c protein-immunopositive cells colabeled for IL-12 p40 RNA; indicating that LPS-stimulated astrocytes

96 tory effect at the transcriptional level for IL-12 p40 without down-regulation of the IL-12 p35 gene.

97 In addition, splenic cytokine mRNA for IL-12 p40, tumor necrosis factor-alpha, and IL-1beta wer

98 10 to 100 microg/ml) was a poor stimulus for IL-12 p40 mRNA expression.

99 The transcript for IL-12 p40 was highly expressed in C3H/HeJ but not C3H/He

100 ally resistant, animals lacking a functional IL-12 p40 gene were found to be highly susceptible to M.

101 o be dependent on CD8(+) T cells, IFN-gamma, IL-12 p40, and Fas ligand.

102 n of (pro)inflammatory cytokines (IFN-gamma, IL-12 p40, and TNF-alpha) and NO in response to myelin b

103 Evaluation of hepatic IL-12 p40 mRNA expression by reverse transcription-PCR a

104 Serum levels of IL-12 heterodimer, IL-12 p40 subunit, IL-4, and Th1 cytokines were determin

105 alysis of the regulation of the cloned human IL-12 p40 promoter.

106 A 3.3-kb human IL-12 p40 promoter construct transfected into cell lines

107 w evidence that full activation of the human IL-12 p40 promoter may result primarily from the interpl

108 ll line RPMI-8866, we localized to the human IL-12 p40 promoter two essential cis elements, the NFkap

109 he CACCC element (-224 to -220) on the human IL-12 p40 promoter was observed in resting human primary

110 curred in monocytes, and involved changes in IL-12 p40 and IL-10 mRNA expression.

111 The decrease in IL-12 p40 levels could be reversed by addition of anti-I

112 This selective defect in IL-12 p40 production was observed regardless of whether

113 s after WNV infection, and mice deficient in IL-12 p40 and IL-23 p40 (Il12b(-/-)) or IL-23 p19 (Il23a

114 macrophages and identify the role of EKLF in IL-12 p40 expression.

115 of the IFN regulatory factor (IRF) family in IL-12 p40 transcription.

116 organisms had induced a 27-fold increase in IL-12 p40 mRNA levels while killed organisms had induced

117 d organisms had induced a 9-fold increase in IL-12 p40 mRNA levels.

118 ere accompanied by substantial reductions in IL-12 p40 and IL-12 p70 protein in both IRF-1(-/-) and I

119 containing an internal CpG motif results in IL-12 p40 secretion.

120 ments are found to have an important role in IL-12 p40 promoter activation by lipopolysaccharide.

121 pression of inflammatory cytokines including IL-12 p40, and depletion of IL-12 p40 from p50(-/-)p65(+

122 d proinflammatory gene expression, including IL-12 p40, TNF-alpha, and inducible nitric oxide synthas

123 d in an enhanced DC activation and increased IL-12 p40 production after infection.

124 2-deficient mice also demonstrated increased IL-12 p40 mRNA levels.

125 eficient mice demonstrate markedly increased IL-12 p40 protein and mRNA expression compared with wild

126 tment in these conditions revealed increased IL-12 p40 homodimer (p80) levels were associated with en

127 acrophages from Cbl-/- mice showed increased IL-12 p40 protein production.

128 ination of endogenous IL-10 led to increased IL-12 p40 production in DCs as well as increased prolife

129 0 in a strain-specific manner, and increases IL-12 p40, IFN-gamma-inducible protein-10, and IFN-gamma

130 ysaccharide (LPS) from B. abortus can induce IL-12 p40 mRNA expression and protein secretion by human

131 stablished from ICSBP-/- mice did not induce IL-12 p40 transcripts, nor stimulated IL-12 p40 promoter

132 biting the ability of H. hepaticus to induce IL-12 p40.

133 Furthermore, IRF-8-induced IL-12 p40 synthesis in RAW264.7 cells was enhanced by do

134 SB203580 strongly blocked CD40-induced IL-12 p40 production in DCs at both mRNA and protein lev

135 We found that CXCL10 induced IL-12 p40 production but reduced IL-10 production in uni

136 gest that inhibition of H. hepaticus-induced IL-12 p40 expression by NF-kappaB subunits is critical t

137 on (IFN)-gamma on lipopolysaccharide-induced IL-12 p40 in murine macrophages and alters IFN-gamma sig

138 P) inhibits lipopolysaccharide (LPS)-induced IL-12 p40 mRNA expression, protein production, and promo

139 ability of IFN-gamma to augment LPS-induced IL-12 p40 mRNA further when both stimuli were present si

140 meter polystyrene beads by monocytes induced IL-12 p40 mRNA.

141 of gram-positive bacteria, potently induced IL-12 p40 gene expression.

142 In addition, Taxol-induced IL-12 p40 mRNA was markedly reduced in Mac-1 knockout ma

143 nt increased the synthesis of CD40-inducible IL-12 p40 but not of bioactive p70.

144 IFN-beta-1b significantly inhibits inducible IL-12 p40 up to 80% and biologically active IL-12 p70 up

145 To address this question, we infected IL-12 p40-/- C57BL/6 mice with Leishmania major, an intr

146 TNF-alpha selectively inhibits IL-12 p40 steady-state mRNA, but not those of IL-12 p35,

147 Interestingly, IL-12 p40 mRNA was also detected in tumor-bearing animal

148 ce express lower levels of gamma interferon, IL-12 p40, and inducible nitric oxide synthetase 2 (NOS2

149 a, and a reduction in spontaneous intestinal IL-12 p40, TNF, IFN-gamma, and IL-17 production.

150 response to LPS, IRF.1-/- mice produced less IL-12 p40, IL-12 p35, and IFN-gamma mRNA in the liver th

151 In contrast, D2 mice had more IL-12 p40 in their lungs than did B6 mice.

152 o a marked induction of both human and mouse IL-12 p40 promoter activities in ICSBP+/+ and ICSBP-/- c

153 trast, anti-huIL-12Rbeta1 mAb 2B10 and mouse IL-12 p40 subunit homodimer (mo(p40)2) blocked 125I-huIL

154 Regulation of the murine IL-12 p40 promoter is complex.

155 Mutations from -79 to -74 in the murine IL-12 p40 promoter significantly reduce lipopolysacchari

156 defective adenoviruses containing the murine IL-12 p40 subunit (Ad.mp40) or a bicistronic vector cont

157 0 days before infection increased lymph node IL-12 p40 productive capacity 20-fold compared to that o

158 ee (SPF)-derived mice produce IL-10, but not IL-12 p40, when activated with enteric bacteria.

159 ility was further supported when we observed IL-12 p40 overexpression selectively in airway epithelia

160 osis during infection, but in the absence of IL-12 p40 and functional FasL, activation of this transc

161 us or LPS also inhibited the accumulation of IL-12 p40 mRNA, but did not inhibit IL-12 p35 and TNF-al

162 ively inhibits transcriptional activation of IL-12 p40 and p35 genes while potently activating IL-10

163 ced signaling pathways for the activation of IL-12 p40 gene expression, or an abnormality in the IL-1

164 P. acnes also induced activation of IL-12 p40 promoter activity via TLR2.

165 pG DNA and LPS with respect to activation of IL-12 p40 secretion.

166 ns play important roles in the activation of IL-12 p40 transcription by bacteria.

167 also plays a role in the ICSBP activation of IL-12 p40.

168 ge of disease express an increased amount of IL-12 p40 mRNA.

169 ther antigen producing equivalent amounts of IL-12 p40 had no effect.

170 f IL-12 and TNF-alpha by splenocytes, and of IL-12 p40 by purified spleen CD8alpha(+) lymphoid dendri

171 The beneficial effect on CIA of IL-12 p40-transduced T cells required TCR specificity ag

172 The bifunctional control of IL-12 p40 by EKLF and its modulation of NFkappaB support

173 results indicate that the local delivery of IL-12 p40 by T cells inhibited CIA by suppressing autoim

174 okines including IL-12 p40, and depletion of IL-12 p40 from p50(-/-)p65(+/-) mice ameliorates H. hepa

175 roduction through selective dysregulation of IL-12 p40 expression.

176 as followed by 75-fold greater expression of IL-12 p40 (as monomer and homodimer).

177 y acidic protein-p40 mice with expression of IL-12 p40 alone remained asymptomatic, with no inflammat

178 eover, MFAg inhibited the mRNA expression of IL-12 p40 and IL-10 as assessed by RNA protection assays

179 lective impairment in the mRNA expression of IL-12 p40 but not IL-1alpha, IL-1beta, IL-1Ra, IL-6, IL-

180 activation of ERK inhibits the expression of IL-12 p40 by inducing c-Fos.

181 his correlates with diminished expression of IL-12 p40 chain RNA.

182 The expression of IL-12 p40 mRNA levels by reverse transcription-PCR corro

183 amma or TNF-alpha, induced the expression of IL-12 p40 mRNA.

184 f IL-10 to control LPS-induced expression of IL-12 p40 was significantly compromised in macrophages l

185 This study underlines the importance of IL-12 p40 monomer (p40) in helping cancer cells to escap

186 L-10 or IL-12p70 but was a potent inducer of IL-12 p40 subunit.

187 Inhibition of the induction of IL-12 p40 by ERK was independent of c-Rel, a known posit

188 tibody blocked both LPS and LTA induction of IL-12 p40 expression.

189 Induction of IL-12 p40 mRNA by LPS was markedly diminished in both IR

190 Induction of IL-12 p40 was even further increased in IL-12 p35-defici

191 MIP-3beta correlated with the inhibition of IL-12 p40 and TNF-alpha production by monocytes and with

192 Inhibition of IL-12 p40 gene expression by c-Maf requires the N-termin

193 un-on assays revealed that the inhibition of IL-12 p40, IL-12 p35, and TNF-alpha was at the gene tran

194 TNF-alpha is a potent inhibitor of IL-12 p40 and p70 secretion from human macrophages induc

195 the induction of c-Fos, a known inhibitor of IL-12 p40 expression.

196 The defect is also observed at the level of IL-12 p40 and p35 mRNA expression.

197 nd concomitant increases in airway levels of IL-12 p40 (as homodimer) and airway macrophages.

198 des expressed up to 10-fold higher levels of IL-12 p40 and inducible (type 2) nitric oxide synthase m

199 charide had markedly reduced serum levels of IL-12 p40 and interferon gamma.

200 olitis is associated with elevated levels of IL-12 p40 expression, and p50/p105-deficient macrophages

201 hepaticus induced markedly higher levels of IL-12 p40 in p50(-/-)p65(+/-) macrophages than in WT mac

202 ficient macrophages express higher levels of IL-12 p40 than wild-type macrophages after challenge wit

203 e of infection had decreased serum levels of IL-12 p40.

204 However, the half-life of IL-12 p40 mRNA was approximately threefold lower in acti

205 ntified as astrocytes, while the majority of IL-12 p40 RNA-expressing cells appeared to be microglia.

206 lts placed epithelial cell overgeneration of IL-12 p40 as a key intermediate for virus-inducible infl

207 ve mice were also examined for production of IL-12 p40 mRNA following exposure to the viable or kille

208 (-/-) mice demonstrated robust production of IL-12 p40.

209 entified to be involved in the regulation of IL-12 p40 gene expression by LPS and IFN-gamma, i.e., c-

210 e was induced by EKLF for down-regulation of IL-12 p40 transcription in activated RAW264.7 cells, but

211 dent of c-Rel, a known positive regulator of IL-12 p40.

212 Radioimmunoassay (RIA) of IL-12 p40 protein on supernatants revealed IL-12 release

213 This study delineates a novel role of IL-12 p40 in inducing the expression of iNOS in microgli

214 ytosis and decreased macrophage secretion of IL-12 p40.

215 play a pivotal role in the superinduction of IL-12 p40 observed after challenge of macrophages lackin

216 ast, iC3b binding resulted in suppression of IL-12 p40 mRNA and significantly inhibited the productio

217 e hypothesize that the impaired synthesis of IL-12 p40 in ICSBP-/- animals is the primary lesion resp

218 s correlated with increased transcription of IL-12 p40.

219 There was dramatic upregulation of IL-12 p40 mRNA following exposure of macrophages to eith

220 ponses in vivo, primarily through the use of IL-12 p40 gene-targeted mice and neutralizing antibodies

221 IFN-gamma serum levels, but had no effect on IL-12 p40, IL-6, or IL-10 serum levels.

222 The inhibitory action of NO on IL-12 p40 is independent of the cytokine IL-10.

223 Nevertheless, in contrast to IFN-gamma-/- or IL-12 p40-/- animals, iNOS-deficient mice survived acute

224 pe I IFN requirement, but not type II IFN or IL-12 p40.

225 e mice deficient in IFN-gamma, IL-12 p35, or IL-12 p40 all succumbed to LVS doses that were sublethal

226 al function for this factor in orchestrating IL-12 p40 production in macrophages.

227 LPS was a more potent inducer of IL-12 p35, IL-12 p40, and IFN-gamma mRNA, as well as of IL-12 prote

228 ased the induction of MPL-induced IL-12 p35, IL-12 p40, and IFN-gamma mRNA, suggesting that the enhan

229 to induce interleukin-10 (IL-10), IL-12 p35, IL-12 p40, gamma interferon (IFN-gamma), glucocorticoid

230 tion of cyclooxygenase 2 (COX-2), IL-12 p35, IL-12 p40, TNF-alpha, IFN-inducible protein (IP)-10, and

231 IL-12 p70 protein paralleled IL-12 p40 protein expression.

232 oluble CD40 ligand agonist failed to produce IL-12 p40 and IL-12 p70 protein; however, the deficient

233 phages for activation of the proinflammatory IL-12 p40 and anti-inflammatory IL-10 promoters, as well

234 e also mitigated in IRF-1-/- mice, pulmonary IL-12 p40 and IL-12 p35 mRNA were not dysregulated.

235 innate and acquired immunity, down-regulate IL-12 p40 and inducible NO synthase expression in LPS/IF

236 ption factor in macrophages able to regulate IL-12 p40 transcription depending on the cellular activa

237 ntly higher mRNA expression of the regulated IL-12 p40 subunit, but not IL-10, in sarcoid compared wi

238 ed with CpG but not control ODN up-regulated IL-12 p40 expression and release, and these effects were

239 gamma R ligation selectively down-regulates IL-12 p40 and p35 gene expression at the level of transc

240 actor which directly or indirectly regulates IL-12 p40 gene activation and, as a consequence, IFN-gam

241 resence or absence of this element repressed IL-12 p40 transcription in interferon gamma/lipopolysacc

242 Dual labeling in situ analysis revealed IL-12 p40 RNA-positive cells scattered throughout the br

243 efficiently phagocytose chlamydiae, secrete IL-12 p40, and present chlamydial antigen(s) to infectio

244 g TNF-alpha by as much as sixfold, but serum IL-12 p40 and IL-12 p70 responses increased by only twof

245 KRV infection increases serum IL-12 p40 in a strain-specific manner, and increases IL-

246 -23) is a heterodimeric cytokine that shares IL-12 p40 but contains a unique p19 subunit similar to I

247 ure restored normal levels of LPS-stimulated IL-12 p40 production.

248 induce IL-12 p40 transcripts, nor stimulated IL-12 p40 promoter activity after IFN-gamma/LPS stimulat

249 ting RAW264.7 cells, whereas EKLF suppressed IL-12 p40 expression in activated RAW264.7 cells.

250 n addition, chloroquine treatment suppressed IL-12 p40 production in response to Legionella treatment

251 the p50/p105 subunit of NF-kappaB suppresses IL-12 p40 expression have not yet been elucidated.

252 pha protein expression occurred earlier than IL-12 p40 protein but was not required for IL-12 inducti

253 In vitro analysis demonstrated that IL-12 p40 suppressed IFN-gamma production in developing

254 Finally, we showed that IL-12 p40-/- mice displayed aberrant negative selection

255 s with multiple sclerosis (MS) suggests that IL-12 p40 may have a role in the pathogenesis of the dis

256 The IL-12 p40 gene product, expressed specifically in macrop

257 The IL-12 p40(-/-) mice were unable to control bacterial gro

258 The IL-12 p40, IFN-gamma, TNF, and IL-6 responses were drama

259 Jun and a mutant c-Jun molecule activate the IL-12 p40 promoter and synergistically activate the prom

260 tic cell lines synergistically activated the IL-12 p40 promoter, apparently overcoming the requiremen

261 We have analyzed the IL-12 p40 promoter, which is induced in macrophages by b

262 ulation of nuclear binding activities at the IL-12 p40 NFkappaB half-site was induced by EKLF for dow

263 redominant Rel dimers capable of binding the IL-12 p40 Rel site were the p50/p65 and p50/c-Rel hetero

264 es of 40, 43, 75, and 120 kDa containing the IL-12 p40 protein.

265 Polymorphisms within the genes encoding the IL-12 p40 subunit, IL12B, and one of the IL-23 receptor

266 The gene for the IL-12 p40 subunit is expressed in macrophages following

267 ding to the regulatory elements found in the IL-12 p40 and inducible NO synthase promoters.

268 40 gene expression, or an abnormality in the IL-12 p40 gene itself.

269 tibody to IL-12 and in mice deficient in the IL-12 p40 gene, ischemia/reperfusion-induced increases i

270 ding to the unique p40 NF-kappaB site in the IL-12 p40 promoter, whereas binding patterns to Ets and

271 in vitro to the unique NF-kappaB site in the IL-12 p40 promoter.

272 infection were both markedly reduced in the IL-12 p40(-/-) mice.

273 roinflammatory cytokine genes, including the IL-12 p40 gene.

274 ptional level, and that the induction of the IL-12 p40 and p35 genes have different requirements for

275 accharide (LPS)-induced transcription of the IL-12 p40 gene, which encodes the heavy chain of the IL-

276 We previously reported an analysis of the IL-12 p40 promoter in RAW264.7 macrophages.

277 Its possible role in the activation of the IL-12 p40 promoter is discussed.

278 l transduction involved in activation of the IL-12 p40 promoter; the phosphorylation defects may be i

279 This cytokine is composed of the IL-12 p40 subunit and a p19 subunit.

280 reus activation caused superinduction of the IL-12 p40, IL-12 p35, and TNF-alpha genes.

281 ad, an additional mechanism dependent on the IL-12 p40 protein, either alone or in another complex su

282 ith IL-12Rbeta1 primarily via regions on the IL-12 p40 subunit and with IL-12Rbeta2 via 20C2-reactive

283 the IL-12 subunits (p35 and p40) or only the IL-12 p40 subunit genes targeted to astrocytes in the mo

284 LPS-induced binding of NF-kappaB to the IL-12 p40 promoter NF-kappaB-binding site was not affect

285 subunit to the nucleus to transactivate the IL-12 p40 gene, ultimately resulting in decreased IL-12

286 l protein binding site (Rel site) within the IL-12 p40 promoter.

287 ant cytokine was transiently administered to IL-12 p40-deficient mice during the first 2 wk of infect

288 Similar to IL-12 p40-deficient mice, IL-12Rbeta1(-/-) mice were imp

289 e role of IL-12 in immunity to tuberculosis, IL-12 p40(-/-) mice were infected with M. tuberculosis a

290 Using IL-12 p40 gene expression as a reporter for CD40 signali

291 rophil influx by 4 h, accompanied by ex vivo IL-12 p40 and p70 release.

292 Similar observations were made when IL-12 p40 knockout mice were used as allograft donors an

293 To determine whether IL-12 p40 homodimer was capable of antagonizing IL-12-de