ライフサイエンスコーパス: IM-MS

1 IM MS(2) experiments provided strong evidence that the m

2 IM-MS data acquired for these two conformers were compar

3 IM-MS data on non-native conformers should therefore be

4 IM-MS distributions of the analogue peptides, when compa

5 IM-MS evidenced ADC multiple drug loading, collisional c

6 IM-MS measurements reveal that there are possibly three

7 IM-MS shows the holo protein to exist in four closely re

8 from 12 to 22 carbons yields [2GA + 2Na](2+) IM-MS profiles with reduced conformer microheterogeneity

9 Two-dimensional (2D) IM-MS trend lines are compared with model polymer system

10 er, our results illustrate how native MS and IM-MS can rapidly assess ADC structural heterogeneity an

11 Consequently, combined LC-MS and IM-MS offer a superior approach for the characterization

12 Hence, LC-MS and IM-MS unveiled complementary compositional insight.

13 Native MS and IM-MS will play an increasing role in next generation bi

14 were collected and analyzed by native MS and IM-MS, assessing the interpretation of each HIC peak.

15 ion mobility-mass spectrometry (IM-MS), and IM-MS/MS in conjunction with computational methods.

16 Application of MALDI-MS, ESI-MS, and IM-MS to the polymer-peptide biomaterial confirmed its c

17 ese results highlight the utility of SID and IM-MS in resolving conformational heterogeneity and yiel

18 Atmospheric-pressure (AP) IM-MS offers an advantage in these studies compared to i

19 te the application of the pulsed nano-ESI AP-IM-MS with enhanced ion sampling for detection of solven

20 sensitivity and soft ion introduction in AP-IM-MS.

21 from a approximately 0.9-m drift tube-based IM-MS platform operated at the same pressure (4 Torr).

22 y of a large heteromeric complex analysed by IM-MS, coupled with integrative modelling, highlights th

23 Collision cross sections (CCSs) measured by IM-MS provide a measure of analyte size.

24 isomers were consequently fully resolved by IM-MS, and the relative ratio of the isomers was determi

25 of using gas-phase structural separations by IM-MS for the characterization of AuNPs, revealing signi

26 trostatic fields commonly used in drift cell IM-MS instruments.

27 ng CCS data is well developed for drift cell IM-MS, while strategies for obtaining CCS values from t-

28 lipids measured using t-wave and drift cell IM-MS, while this improves to <0.5% when drift cell phos

29 CCS calibrants measured by MALDI drift cell IM-MS.

30 .7%, respectively, whereas the hybrid MS-CID-IM-MS approach yields amino acid sequence coverages of 8

31 14.3 kDa) demonstrates the ability of MS-CID-IM-MS to rapidly identify the presence and sites of modi

32 The MS-CID-IM-MS top-down approach allows for greater depth of info

33 n proteomics is described, denoted as MS-CID-IM-MS.

34 etter resolved than with existing commercial IM-MS platforms.

35 wever, most of the currently used commercial IM-MS instruments utilize a nonuniform traveling wave fi

36 Here, we describe a method that couples IM-MS and surface-induced dissociation (SID) to dissocia

37 yogenic ion mobility-mass spectrometry (cryo-IM-MS) measurements.

38 yogenic ion mobility-mass spectrometry (cryo-IM-MS) show that dehydration of alkyl diammonium cations

39 er illustrate that a combined data-dependent IM-MS/MS approach for phosphopeptide screening would hav

40 ity mass spectrometry-mass spectrometry (DIA-IM-MS) was used to investigate the allergen composition

41 DT IM-MS, in combination with molecular dynamics (MD), show

42 rift time ion mobility mass spectrometry (DT IM-MS) in addition to circular dichroism spectroscopy.

43 H 6.8 with 20 mM ammonium acetate, in the DT IM-MS instrument, each buffer gas can yield a different

44 m CCS measured in helium via drift tube (DT) IM-MS.

45 DT-IM-MS experiments at 200 K resolve multiple conformers.

46 t time ion mobility mass spectrometry (VT-DT-IM-MS).

47 nization ion mobility mass spectrometry (ESI IM-MS) and molecular dynamics (MD) simulations reveal ne

48 Finally, we present an ESI-IM-MS methodology to determine if a given protein is str

49 been unequivocally characterized by NMR, ESI-IM-MS, and TEM techniques.

50 nization ion mobility-mass spectrometry (ESI-IM-MS) and collision-induced unfolding (CIU) analysis of

51 nization-ion mobility-mass spectrometry (ESI-IM-MS), successfully demonstrates the first evidence for

52 rating that distributions observed using ESI-IM-MS unambiguously reflect the ensemble of conformers o

53 VCFD-ESI-IM-MS yields novel biophysical insight into the influenc

54 tion: "If the only technique you had was ESI-IM-MS, what information would it provide on the structur

55 It remains unclear to what extent IM-MS is suitable for exploring structural properties of

56 Advantages of LC/IM-MS and FIA/IM-MS include the ability to develop mobility-mass trend

57 des: flow injection analysis with IM-MS (FIA/IM-MS), LC/MS, and LC/IM-MS.

58 Finally, IM-MS can be used to gain insights into the conformation

59 lishes a new type of inorganic calibrant for IM-MS allowing sizing, structural analysis, and discover

60 Because comparing CCS derived from IM-MS data with 3D-computed CCS is critical for thorough

61 The use of CCSs, measured from IM-MS and molecular modeling information, for the struct

62 t of a wide range of contemporary and future IM-MS experiments.

63 re, using this data set, we demonstrated how IM-MS can be used to conveniently characterize and ident

64 This Review critically describes how IM-MS has been used to enhance various areas of chemical

65 Our results illustrate how IM-MS can rapidly assess bsAb structural heterogeneity a

66 Despite recent advances in IM-MS instrumentation, the resolution of closely related

67 ther tested the feasibility of incorporating IM-MS into conventional LC/MS metabolomics workflows.

68 atography/ion mobility/mass spectrometry (LC-IM-MS) data, providing a route to quantify ion mobility

69 alysis with IM-MS (FIA/IM-MS), LC/MS, and LC/IM-MS.

70 Advantages of LC/IM-MS and FIA/IM-MS include the ability to develop mobil

71 rift tube ion mobility-mass spectrometer (LC/IM-MS) was evaluated for its utility in global metabolom

72 es can be preserved in the gas phase, making IM-MS a powerful approach for a range of bioanalytical a

73 zation ion mobility mass spectrometry (MALDI-IM-MS) allows a pixel-by-pixel classification and identi

74 zation-ion mobility-mass spectrometry (MALDI-IM-MS) was used to analyze low mass gold-thiolate fragme

75 xperiments are similar to those used in many IM-MS instruments, therefore, the outcomes of this resea

76 ation of mass spectrometry and ion mobility (IM-MS), are also instructive in exploring conformational

77 pectrometry (MS) and native ion mobility MS (IM-MS) is compared to hydrophobic interaction chromatogr

78 Native IM-MS was next used for the first time to characterize a

79 ization-ion mobility-mass spectrometry (nESI-IM-MS).

80 The assessment of IM-MS data, however, is currently impeded due to the lac

81 A semiquantitative interpretation of IM-MS data was developed to directly extrapolate average

82 biomolecules, however, the full potential of IM-MS in their study has yet to be realized due to a lac

83 etry (IM-MS) have accelerated the utility of IM-MS in untargeted, discovery-driven studies in biology

84 Altogether, optimized IM-MS settings allowed a 0.4 nm(2) increase (i.e., 2%) o

85 After initial optimization, the SLIM IM-MS module provided about 5-fold higher resolution sep

86 The high resolution achieved in the TW SLIM IM-MS enabled, e.g., isomeric sugars (lacto-N-fucopentao

87 ation source ion mobility-mass spectrometer (IM-MS) instrument platform for investigations that criti

88 An ion mobility-mass spectrometer (IM-MS) interface is described that can be employed to pe

89 acterized by ion mobility mass spectrometry (IM MS) and tandem mass spectrometry (MS(2)).

90 n (ESI) with ion mobility-mass spectrometry (IM-MS) allows structural studies on biological macromole

91 mbination of ion-mobility mass spectrometry (IM-MS) and hydrogen/deuterium exchange mass spectrometry

92 ch utilizing ion mobility-mass spectrometry (IM-MS) and tandem mass spectrometry (MS/MS) coupled with

93 on (LDI) and ion mobility mass spectrometry (IM-MS) are applied to study molecular weight distributio

94 Ion mobility-mass spectrometry (IM-MS) can provide orthogonal information, i.e., m/z and

95 Ion mobility-mass spectrometry (IM-MS) can thus act as a tool to separate complex mixtur

96 sulting from ion mobility-mass spectrometry (IM-MS) experiments provide a promising orthogonal dimens

97 ing CID with ion mobility mass spectrometry (IM-MS) for dispersing fragment ions along charge state s

98 Ion mobility-mass spectrometry (IM-MS) has gained considerable attention for detection o

99 ation of ion mobility and mass spectrometry (IM-MS) has greatly enlarged the potentials for biomolecu

100 m ion mobility coupled to mass spectrometry (IM-MS) have accelerated the utility of IM-MS in untarget

101 lision cross section with mass spectrometry (IM-MS) helps, but many isomers are still difficult to se

102 Ion mobility-mass spectrometry (IM-MS) in combination with molecular modeling offers the

103 c drift tube ion mobility-mass spectrometry (IM-MS) instrument.

104 lable hybrid ion mobility-mass spectrometry (IM-MS) instruments in 2006, IMS technology became readil

105 Ion mobility-mass spectrometry (IM-MS) is a powerful technique for structural characteri

106 coupled with ion mobility mass spectrometry (IM-MS) is a powerful tool for determining the stoichiome

107 Ion mobility-mass spectrometry (IM-MS) is a technology of growing importance for structu

108 Unique to ion mobility mass spectrometry (IM-MS) is the ability to provide collision cross section

109 e feature of ion mobility mass spectrometry (IM-MS) lies in its ability to provide experimental colli

110 Ion mobility-mass spectrometry (IM-MS) provides rapid two-dimensional separations based

111 Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides hav

112 r structural ion mobility-mass spectrometry (IM-MS) studies is demonstrated using model peptide ions

113 A recent ion mobility-mass spectrometry (IM-MS) study of the nonapeptide bradykinin (BK, amino ac

114 olding (CIU) ion mobility-mass spectrometry (IM-MS) that ncUbq exhibits structural preferences and in

115 derived from ion mobility mass spectrometry (IM-MS) to build three-dimensional models of one form of

116 n the use of ion mobility mass spectrometry (IM-MS) to investigate conformations of proteins and prot

117 Ion mobility mass spectrometry (IM-MS) was used to probe the structures of several metal

118 Ion-mobility mass spectrometry (IM-MS) was used to separate these structures and, signif

119 nization and ion mobility mass spectrometry (IM-MS) were unsuccessfully used in order to resolve the

120 obtained by ion mobility-mass spectrometry (IM-MS), 2D NMR spectroscopy, and computational methods.

121 Recently, ion mobility-mass spectrometry (IM-MS), a technique in which ions are separated accordin

122 opy, ESI-MS, ion-mobility mass spectrometry (IM-MS), AFM, and TEM.

123 issociation, ion mobility mass spectrometry (IM-MS), and density functional theory (DFT) has been use

124 try (MS/MS), ion mobility-mass spectrometry (IM-MS), and IM-MS/MS in conjunction with computational m

125 y ionization-ion mobility-mass spectrometry (IM-MS), collision-induced dissociation (CID), and hydrog

126 plemented as ion mobility-mass spectrometry (IM-MS), comprises two sequential, gas-phase dispersion t

127 Ion mobility-mass spectrometry (IM-MS), tandem mass spectrometry (MS/MS), and computatio

128 es employing ion-mobility mass spectrometry (IM-MS), the apparent controversy is resolved.

129 utility with ion-mobility mass spectrometry (IM-MS), the use of RP-MS data to help model protein comp

130 antly, using ion mobility-mass spectrometry (IM-MS), we find that all four macromolecular complexes r

131 coli, using ion mobility mass spectrometry (IM-MS), which reports gas-phase collision cross-sections

132 The use of ion mobility-mass spectrometry (IM-MS), which separates ions in the gas phase based on t

133 (MS(2)), and ion mobility mass spectrometry (IM-MS).

134 ins based on ion mobility-mass spectrometry (IM-MS).

135 using native ion mobility-mass spectrometry (IM-MS).

136 ion of MS(2) with ion mobility spectrometry (IM-MS(2)) and lead to a strategy to distinguish alpha- a

137 The multipass SUPER IM-MS provided resolution approximately proportional to

138 Variable-temperature IM-MS is here shown to be an exciting approach to discer

139 The results present that IM-MS and molecular modeling can inform on the identity

140 We show that IM-MS/MS analysis can rapidly and accurately differentia

141 The IM MS/MS(2) methods provide a means to analyze, based on

142 The IM-MS instrument operation is independent of which ioniz

143 The IM-MS interface consists of a stacked-ring ion guide des

144 turn, qualifies as a means in evaluating the IM-MS data.

145 From the IM-MS data coupled with theoretical calculations, it was

146 and collisional activation processes in the IM-MS interface are described as a function of the ion-n

147 Integrating the IM-MS and MD data provides a global view that shows step

148 DFT structures, in good agreement with the IM-MS cross sections, indicate two "bent" conformations

149 tion (10-30 mus) ion pulses for use with the IM-MS.

150 d capillary reverse phase columns coupled to IM-MS.

151 The results also suggest that the total IM-MS distribution for a protein is the complex result o

152 In this work, both DT and TW IM-MS instruments are used to investigate the effects of

153 mmon to use nitrogen as the buffer gas in TW IM-MS instruments and to calibrate by extrapolating from

154 oglobin, frequently used as a standard in TW IM-MS studies.

155 ling wave ion mobility mass spectrometry (TW IM-MS) instrumentation rely on the use of calibrants to

156 3 and 4 which demonstrate their use as a TW-IM-MS calibrant set to facilitate characterization of ve

157 Traveling wave IM-MS (TW-IM-MS) has recently become commercially available to non

158 ling-wave ion mobility-mass spectrometry (TW-IM-MS).

159 inally, as proof of concept, we used UPLC-TW-IM-MS to compare the cellular metabolome of epithelial a

160 liquid chromatography (UPLC) coupled with TW-IM-MS.

161 ristearates were, however, not observed upon IM-MS.

162 By using IM-MS, we could detect the native mass of MscL from Esch

163 ucture-function relationships of drugs using IM-MS.

164 structural isomers of drug metabolites using IM-MS is demonstrated and, in addition, a molecular mode

165 y monitoring the conformer preferences using IM-MS.

166 Utilizing IM-MS, statistical analysis, and targeted ultraperforman

167 UVPD-IM-MS analysis serves both as a method for peptide seque

168 VT-IM-MS is used here to investigate the change in the conf

169 tures of this instrument and results from VT-IM-MS experiments on a range of model systems-IMS CCS st

170 mperature ion mobility mass spectrometry (VT-IM-MS) to study the effect of temperature on the stabili

171 ategies for obtaining CCS values from t-wave IM-MS data remains an active area of research.

172 ly from thin tissue sections by MALDI t-wave IM-MS using CCS calibrants measured by MALDI drift cell

173 Traveling wave IM-MS (TW-IM-MS) has recently become commercially availa

174 rogen on the widely available traveling wave IM-MS (TWIM-MS) platform.

175 ially available drift tube or traveling wave IM-MS platforms.

176 Recently, traveling-wave (t-wave) IM-MS was developed which uses electrodynamic rather tha

177 l, we explored in the present report whether IM-MS can be used to differentiate close conformers and

178 With IM-MS, the total surfactant ions were separated accordin

179 peration modes: flow injection analysis with IM-MS (FIA/IM-MS), LC/MS, and LC/IM-MS.

180 c conformational tightening, consistent with IM-MS data.

181 Combining RAPTOR with IM-MS and collision-induced dissociation (CID) enables u