ライフサイエンスコーパス: IPTG

1 IPTG treatment caused parallel increases in the levels o

2 IPTG-induced C/EBPalpha caused inhibition of cell prolif

3 IPTG-induced pilin rescued S motility of the DeltapilA s

4 very low inducer concentrations (3-10microM IPTG) and reached levels significantly higher than in st

5 ive in the Lon and OmpT proteases and allows IPTG-inducible expression of recombinant proteins from t

6 An IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible

7 mevalonate pathway genes is regulated by an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible

8 of BosR in B. burgdorferi, we constructed an IPTG (isopropyl-beta-d-thiogalactopyranoside)-regulated

9 In this study we have constructed an IPTG-inducible GFP expression system in M. tuberculosis

10 ichia coli RNA metabolism, we constructed an IPTG-inducible pcnB [poly(A) polymerase I, PAP I] contai

11 corresponds closely to that observed for an IPTG-inducible A14 recombinant whose construction and ch

12 n of the 14 genes in the bfp cluster from an IPTG-inducible promoter, in the absence of pJPN14, is su

13 that conditionally expressed FbpABC from an IPTG-inducible promoter.

14 tants and analyzed their activities using an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible

15 Additionally, assays with an IPTG-regulatable bb0795 mutant revealed that BB0795 is r

16 t was fully reversible by the lactose analog IPTG.

17 Addition of a lactose analog, IPTG, to the swimming water of the axolotl is sufficient

18 lets, we study the diffusion of both AHL and IPTG from inducer-filled reservoirs into bacteria-contai

19 Engineered bacteria that integrate AHL and IPTG signals with a synthetic AND gate gene circuit are

20 ilizes but does not eliminate the loops, and IPTG does not redistribute loops among high-FRET topolog

21 When cells were treated with both Tc and IPTG, full induction of cat was reached in 24 h and main

22 1 phosphatase is regulated experimentally by IPTG (isopropyl-beta-D-thiogalactopyranoside) (35).

23 sense or pUC19 sequences could be induced by IPTG were also constructed.

24 d two-fold when transcription was induced by IPTG, but had no detectable effect on protein yield when

25 sformation competence was also influenced by IPTG levels in the growth medium.

26 were rescued from those plasmids that caused IPTG-dependent cell death.

27 In contrast, in these cells, IPTG-dependent induction of p14ARF, which sequesters MDM

28 Also, the conditional IPTG inducible expression of wild type Sgk, but not of t

29 D149 contacts IPTG directly, and variations at this site provide the o

30 cted from the immunoprecipitate demonstrated IPTG (isopropylthiogalactoside)-sensitive binding of GFP

31 lot analysis, we demonstrated dose-dependent IPTG induction of LLO during growth in broth culture.

32 tested: five time points and three different IPTG and 3-AT concentrations.

33 Fusions exhibiting IPTG-responsive beta-galactosidase activity were cloned

34 Following IPTG (isopropyl-beta-D-thiogalactopyranoside) induction,

35 that endogenous p21 mRNA increased following IPTG removal, which may be responsible for the continued

36 hey contained increased Tat levels following IPTG induction.

37 cell paste of R.XmnI was obtained following IPTG induction in a suitable E. coli host.

38 lative gene expression changes when RNA from IPTG-induced cells was labeled and compared to RNA from

39 lta virB operon mutant expressing virB7 from IPTG-inducible Pklac and virB9 from an AS-inducible Pvir

40 M isopropyl-1-thio-beta-D-galactopyranoside (IPTG) induced the expression of Ha-Ras(Val12), closely f

41 nder isopropylthio-beta-D-galactopyranoside (IPTG)-inducible control.

42 h isopropyl-1-thio-beta-D-galactopyranoside (IPTG).

43 or isopropyl-beta-D-thio-galactopyranoside (IPTG) can be utilized for the implementation of an artif

44 inhibitor isopropyl thio-beta-D-galactoside (IPTG) requires the mutant enzyme to adopt its less favor

45 dition of isopropyl-beta-D-thio-galactoside (IPTG) destabilizes but does not eliminate the loops, and

46 tion of ERK1/2 and the induction of COX-2 in IPTG-induced Rat-1:iRas cells.

47 No free-citrate transport was observed in IPTG (isopropyl-beta-d-thiogalactopyranoside)-induced or

48 lls expressing a non-mutated Fd (UMHC) or in IPTG-treated non-transduced V79 cells.

49 d retroviral vector, MSCV-In(S), resulted in IPTG-responsive oncogene expression and cell growth.

50 For L148F, inducer IPTG binding affinity is strengthened, whereas O(1) oper

51 to increasing concentrations of the inducer IPTG (isopropyl-beta-D-thiogalactopyranoside) and were a

52 ur when LacI binds either DNA or the inducer IPTG, or both.

53 biomaterials to present the genetic inducer, IPTG, with different modes of activating genetic circuit

54 recombinant virus in which F18 expression is IPTG (isopropyl-beta-d-thiogalactopyranoside) dependent,

55 the control of the Pspac promoter, which is IPTG inducible.

56 her involves isopropylthiogalactopyranoside (IPTG)-induced synthesis of sigma 32 at 30 degrees C from

57 This promoter, LTRo, was shown to mediate IPTG-inducible cat expression in rat cells expressing th

58 on of expression at 16 degrees C with 10 muM IPTG yields 80% monomeric cyclic protein.

59 ollowing infection of mice in the absence of IPTG or d-alanine, the bacterium survived in vivo for le

60 croscopy of cells infected in the absence of IPTG revealed normal-appearing crescents and immature vi

61 nslocate through the block until addition of IPTG induces repressor dissociation.

62 scape into the cytosol following addition of IPTG to the cell culture medium, thus yielding the abili

63 Rec- and Rec+ by the removal or addition of IPTG.

64 MOPS) medium containing different amounts of IPTG.

65 In addition, systemic applications of IPTG were used to induce genetic circuits in cells encap

66 The binding of IPTG to native (tetrameric) LacI-oDNA complexes also app

67 directly correlate with the concentration of IPTG (isopropyl-beta-D-thiogalactopyranoside) in the gro

68 lacY mutant and varying the concentration of IPTG.

69 ystem II and system I, both under control of IPTG, we have begun to study the capabilities and charac

70 led in the murine retina by oral delivery of IPTG over three induction-repression cycles.

71 expression increased smoothly as the dose of IPTG added to the culture was increased.

72 y inducible trc promoter, the elimination of IPTG, the inclusion of the tetracycline resistance gene,

73 nd pilin protein in response to the level of IPTG in the growth medium.

74 An increase in the level of IPTG induced a sphere-to-short rod transition that re-es

75 ac-fnr delta resDE mutant in the presence of IPTG indicated that resDE has an additional role in B. s

76 The particles prepared in the presence of IPTG were more infectious than those prepared in its abs

77 te starvation conditions, in the presence of IPTG, all strains containing mutated phoR genes showed a

78 ict dose-dependent growth in the presence of IPTG.

79 Removal of IPTG reverted the cell to full G6PD activity.

80 In the absence of an inducer (allolactose or IPTG) this strain, DH1 lackan , cannot grow on kanamycin

81 s concentrations of tetracycline (Tc) and/or IPTG.

82 Hence, treatment with either Tc or IPTG led to a tenfold increase in CAT activity.

83 ion could be alleviated by exposure to Tc or IPTG, which inhibited the binding activities of TTA and

84 plasmid vector with each cDNA having its own IPTG-inducible promoter.

85 sed a fusion of rpoD to the LacI-repressible IPTG-inducible promoter, Pspac, to vary the levels of si

86 , even after removal of the inducing signal (IPTG).

87 d induced by the non-metabolizable substrate IPTG.

88 Metabolic labelling of newly synthesized IPTG-induced proteins during/absence of proteasomal inhi

89 oes not reduce FRET further, suggesting that IPTG stabilizes extended or other low-FRET loops.

90 e consistency in the phenotypes seen for the IPTG- and TET-inducible recombinants confirms the effica

91 The protein was overexpressed in the IPTG-inducible vector pET14b-TnsD in E. coli BL21(DE3)-R

92 ription, fnr was placed under control of the IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible

93 spx gene was placed under the control of the IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible

94 ilis strain carrying a fusion of rodA to the IPTG-inducible Pspac promoter is inducer dependent.

95 se isopropyl beta-d-1-thiogalactopyranoside (IPTG) induction.

96 e, Isopropyl beta-D-1-thiogalactopyranoside (IPTG) that has no off-target effects in mammals.

97 of isopropyl beta-D-1-thiogalactopyranoside (IPTG)-inducible mutant T7 promoters of varied strength a

98 r, isopropyl beta-D-1-thiogalactopyranoside (IPTG).

99 er isopropyl beta-D-1-thiogalactopyranoside (IPTG, which inhibits the interaction between the lac rep

100 t on isopropyl-beta-D-thiogalactopyranoside (IPTG) for expression has been inserted into the genome.

101 sing isopropyl beta-D-thiogalactopyranoside (IPTG) in a bacterial strain that harbors the lacIq gene,

102 rief isopropyl-beta-D-thiogalactopyranoside (IPTG) treatment elevated lacZ, lacY, and lacA transcript

103 with isopropyl-beta-D-thiogalactopyranoside (IPTG) were constructed.

104 Isopropyl-beta-D-thiogalactopyranoside (IPTG), an inhibitor of the lac repressor, can prevent de

105 with isopropyl beta-d-thiogalactopyranoside (IPTG), we were able to detect 10 CFU.mL(-1) in drinking

106 f an isopropyl-beta-D-thiogalactopyranoside (IPTG)-dependent promoter.

107 d an isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible M. mycoides subsp. mycoides SC biotype p

108 from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Plac and other virB genes from acetosyri

109 ated isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible promoter present on a multicopy plasmid.

110 An isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoter was constructed in Myxococcus x

111 f an isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible promoter.

112 from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoters for the purpose of continuousl

113 from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoters.

114 m an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible yirB construct.

115 g an isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoter upstream of the ribosomal bindi

116 t on isopropyl-beta-D-thiogalactopyranoside (IPTG).

117 with isopropyl-beta-D-thiogalactopyranoside (IPTG).

118 log, isopropyl-beta-D-thiogalactopyranoside (IPTG).

119 d by isopropyl-beta-D-thiogalactopyranoside (IPTG).

120 ucer isopropyl-beta-D-thiogalactopyranoside (IPTG).

121 with isopropyl-beta-D-thiogalactopyranoside (IPTG).

122 isopropyl-beta-delta-thiogalactopyranoside (IPTG) inducible promoter, and transfected and then expre

123 inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pai

124 the inducer isopropyl-beta-thiogalactoside (IPTG), repression was relieved.

125 he inducer isopropyl-beta-D-thiogalactoside (IPTG), iA6 replicated normally, and membrane proteins of

126 ddition to isopropyl-beta-d-thiogalactoside (IPTG), to the growth medium of the E. coli expression ho

127 with the data for isopropyl thiogalactoside (IPTG), but somewhat discrepant with respect to the data

128 ression by 0.5 mM isopropyl thiogalactoside (IPTG).

129 erepression of the transposase gene prior to IPTG induction.

130 macrophages, M. tuberculosis that respond to IPTG with expression of GFP fluorescence, and hence are

131 osis that fail to express GFP in response to IPTG, and hence are metabolically inactive, reside withi

132 otype pyruvate dehydrogenase region, and two IPTG-independent polypeptides, of 29 kDa and 57 kDa, wer

133 nges occur in the R3 dimer-oDNA complex upon IPTG binding, indicative of small adjustments in the ori

134 e cell line J774 was strictly dependent upon IPTG.

135 nt of view, since it is not necessary to use IPTG.

136 ted genes independently of the commonly used IPTG and arabinose strategies.

137 usion protein was expressed in E. coli using IPTG induction and purified using FMSN-Ni-NTA.

138 sform up to 5 mM of phenol and tyrosol using IPTG (isopropyl-beta-D-thiogalactopyranoside) as inducer

139 cell spread of iLLO bacteria in L2 cells was IPTG dose dependent.

140 l induction was reached after 192 h, whereas IPTG addition led to full induction within 24 h.

141 O(1) binding is increased for S151P, whereas IPTG binding is decreased.

142 iskly induced by treatment of the cells with IPTG (EC50 13.7 microM).

143 te rhamnose-induced expression compared with IPTG-induced expression from lacp.

144 ne circuits were activated in materials with IPTG embedded within the scaffold walls or chemically li

145 ither native promoter or Pspac promoter with IPTG induction, resulted in a similar level of alkaline

146 Unless supplemented with IPTG (which induces expression of dapD) or DAP, these ce

147 Combined treatment with IPTG and TGF-beta1 resulted in a 20-50-fold increase in

148 Combined treatment with IPTG and TGF-beta1 synergistically increased the lucifer

149 Treatment with IPTG resulted in high level induction of the lacZYA and

150 biotransformation rate was attained without IPTG, confirming that this fragment encodes for a phenol