ライフサイエンスコーパス: IRBP

1 IRBP is confined to the IPM, being too large to diffuse

2 IRBP is known to bind visual retinoids.

3 IRBP is rapidly removed from the zebrafish eye (half lif

4 IRBP message was PCR amplified from these cells after mi

5 IRBP was expressed in the liver within hours of administ

6 IRBP-induced T cells from IRBP-immunized TCR-delta(-/-)

7 IRBP-knockout (KO) and wild-type (WT) mice on a highly E

8 IRBP-specific CD4(+)CD25(high) T cells could be cultured

9 IRBP-specific T cells activated by polyI:C-treated RACs

10 IRBP-specific T cells preferentially expressed IL-17 whe

11 ration with a slope of 0.012 min(-1) mum(-1) IRBP also removed all-trans-retinal, but with much less

12 T) and miR-155(-/-) mice, while miR-155(-/-) IRBP-specific T cells did not.

13 kines, and increased the numbers of IL-17(+) IRBP T cells in the inflamed eye.

14 or retinoid-binding protein peptide 161-180 (IRBP(161-180)).

15 Subretinal delivery of AAV5-IRBP/GNAT2-DIO3, which directs expression of human DIO3

16 n APC that promotes or selectively activates IRBP-specific FoxP3+ TGF-beta+ CD25+CD4+ Treg cells in t

17 -binding protein/complete Freund's adjuvant (IRBP/CFA) or adoptive transfer of T cells.

18 40-activating antibody on days 0 and 4 after IRBP(161-180) sensitization or on days 10 and 14 of uvei

19 3- to 4-fold greater Th17 responses against IRBP in Daf1(-/-) mice with EAU, and they expressed sign

20 Unexpectedly, not only WT but also IRBP KO mice immunized with a uveitogenic regimen of IRB

21 ated, therefore, that a mechanism such as an IRBP membrane receptor in the apical plasma membrane may

22 that Sp1 interactions are facilitated by an IRBP.

23 aluated the chemotactic activity of S-Ag and IRBP and found that both induced migration of human and

24 solated retinas and eyecups from control and IRBP-deficient mice.

25 y and potently suppressed CRX expression and IRBP promoter activity, as measured by RT-PCR and transi

26 Retinal histology and IRBP-specific T-cell responses were compared after 14 da

27 he chemotactic activity of LTB4 on naive and IRBP-specific autoreactive T cells as well as effector l

28 phenotypes in mice lacking both peropsin and IRBP with those of mice lacking peropsin or IRBP alone a

29 The interaction between local RACs and IRBP-specific T cells was regulated by a distinct patter

30 5 repressed transactivation of rhodopsin and IRBP promoters alone and in combination with the transcr

31 anscriptional repressor of the rhodopsin and IRBP promoters in vitro and, in the retina, is a possibl

32 rences in epitope recognition between WT and IRBP KO mice were observed in C57BL/6 mice, but not in B

33 apical and basal media in the absence of apo IRBP.

34 ected in the apical medium but only when apo IRBP was present there.

35 one specific for the immunizing autoantigen (IRBP-Th17) and a much more abundant type (bystander-Th17

36 A subset of autoreactive IRBP-specific CD8 T cells expressed IL-17.

37 e presence of a specific interaction between IRBP and the rod outer segment, probably mediated by a r

38 uency of activated T cells specific for both IRBP tetramers in Aire(-/-) mice, but not in Aire(+/+) m

39 that AR could be a novel mediator of bovine IRBP-induced uveitis in rats.

40 or a strong T cell response, whereas bovine IRBP is a strong inducer of experimental autoimmune uvei

41 zed with peptide 1-20 or with whole (bovine) IRBP.

42 patients with retinitis pigmentosa caused by IRBP mutation.

43 oval from the outer segment is determined by IRBP concentration, whereas the effect of serum albumin

44 Ocular inflammation induced by IRBP peptide immunization and specific T cell transfer w

45 ple pathogenic events of uveitis mediated by IRBP-specific uveitogenic T cells, including the activat

46 Immunization of rats by IRBP peptide resulted in a significant infiltration of l

47 AU-prone B6 mouse activates both CD4 and CD8 IRBP-specific T cells.

48 ported the proliferation and survival of CD8 IRBP-specific T cells, while others had only a growth-pr

49 ent study, we determined the role of the CD8 IRBP-specific T cells in the pathogenesis of experimenta

50 The eyes of congenic IRBP knockout (KO) and C57BL/6J wild-type (WT) mice rang

51 In 6- to 8-month-old Chm(Flox), Tyr-Cre+, IRBP-Cre+ mice, the degeneration of photoreceptors was a

52 In addition to its role in the visual cycle, IRBP is needed for normal eye development.

53 D1080N IRBP was not transported to the Golgi apparatus, but acc

54 icantly increased in cells expressing D1080N IRBP.

55 itutions at positions 304 and 1175 in D1080N IRBP promoted secretion of the mutated IRBP.

56 Moreover, D1080N IRBP induced up-regulation and nuclear translocation of

57 s, significantly rescued secretion of D1080N IRBP, suggesting that misfolding is the molecular basis

58 involved in the disease mechanisms of D1080N IRBP.

59 ant throughout the day, the enhanced daytime IRBP mRNA expression may function to compensate for an i

60 e DAF protein treatments exhibited decreased IRBP-specific Th1/Th17 responses and were protected from

61 e without TAM receptor spontaneously develop IRBP-specific CD4(+) T cells and are more susceptible to

62 overlapping peptides representing the entire IRBP molecule, and lymphocyte proliferative responses we

63 re in C57BL/6 mice by endogenously expressed IRBP.

64 ors was accelerated compared with Chm(Flox), IRBP-Cre+ mice but became leveled with age, such that it

65 In Chm(Flox), IRBP-Cre+ mice the authors observed the progressive dege

66 Chm(Flox), Tyr-Cre+ and Chm(Flox), IRBP-Cre+ mice were mated to produce mice with Chm KO in

67 suggest that endogenous CRX is required for IRBP promoter activity in retinoblastoma cells.

68 he cone matrix sheath may be responsible for IRBP-mediated cone targeting of 11-cis retinoids.

69 th a small number of gammadelta T cells from IRBP-immunized C57BL/6 mice restored the disease-inducin

70 IRBP-induced T cells from IRBP-immunized TCR-delta(-/-) mice on the C57BL/6 geneti

71 CD4(+) lymph node cells from IRBP-immunized WT mice transferred EAU to naive wild-typ

72 Interestingly, APCs or DCs isolated from IRBP-immunized dko mice exhibited a greater ability to d

73 hodopsin and four novel nuclear markers from IRBP, EGR1, EGR2B and EGR3 that we developed through an

74 Furthermore, IRBP secreted by the photoreceptors is taken up from the

75 IL-17+, but not the alphabetaTCR+IFN-gamma+, IRBP-specific T-cell responses was correlated to the per

76 hat adoptive transfer of alpha-MSH-generated IRBP-specific Treg cells promotes retinal allograft surv

77 How IRBP mediates ocular development is unknown.

78 Human IRBP peptide 1-20 contains a major epitope for the H-2b

79 itope contained in residues 1 to 20 of human IRBP that induces EAU in H-2b mice.

80 A mutation in the human IRBP has been linked to retinitis pigmentosa, a progress

81 ted virus-2/5-vectored human RPGR with human IRBP or GRK1 promoters, in vivo imaging showed preserved

82 EAU promoted regulatory T-cell activation in IRBP-specific effector T cells from the spleens of EAU-r

83 tokine production and regulatory activity in IRBP-specific T cells from wild-type or MC5r-/- mice ass

84 pathway(s) leading to photoreceptor death in IRBP-deficient mice remains poorly understood.

85 Recently, a D1080N mutation in IRBP was found in patients with retinitis pigmentosa, a

86 ecific, as their activity was not present in IRBP KO mice immunized with IRBP in incomplete Freund's

87 of retinal HO-1 mRNA and with a reduction in IRBP transcription.

88 Despite the known rhythmicity in IRBP mRNA expression, neither the amount of IRBP nor its

89 DAF significantly influences IRBP-specific Th1 and Th17 responses and disease severit

90 istry showed that the apparent intercellular IRBP in both the RPE and the photoreceptors is resistant

91 gulation of these two processes, because its IRBP mRNA levels are under circadian regulation.

92 in vivo uptake of [(35)S]methionine-labeled IRBP was light independent.

93 Mice lacking IRBP display severe early and progressive photoreceptor

94 m(Flox) was crossed with the transgenic line IRBP-Cre to achieve Chm KO, specifically in the photorec

95 unoelectron microscopy were used to localize IRBP in intact and detached retina-retinal pigment epith

96 es comparable to those induced by 100 microg IRBP.

97 is differential DNA methylation may modulate IRBP gene expression since exogenous methylation of the

98 e (CAT) reporter plasmids containing a mouse IRBP promoter and AODNs directed against CRX.

99 specific cytosines in the bovine and murine IRBP promoters are unmethylated in photoreceptive cells

100 1080N IRBP promoted secretion of the mutated IRBP.

101 induced with the epitope or with the native IRBP protein.

102 These EAU-relevant T reg cells were not IRBP specific, as their activity was not present in IRBP

103 ch were exhibited high sensitivity to ocular IRBP autoantigens.

104 In addition, the ability of IRBP-specific T cells to interact with RACs was dependen

105 nol supplies are disrupted in the absence of IRBP.

106 IRBP mRNA expression, neither the amount of IRBP nor its localization changes significantly during t

107 Because the amount of IRBP remains constant throughout the day, the enhanced d

108 The amounts of IRBP in the RPE at middark and midlight were the same.

109 sults reveal a wash-resistant association of IRBP with a matrix domain immediately surrounding cone o

110 Here, we explore the association of IRBP with the interphotoreceptor matrix (IPM) of cones v

111 pportunity to uncover the molecular basis of IRBP's role in this process.

112 d time of day on the compartmentalization of IRBP were characterized by quantitative Western blot ana

113 The effect of different concentrations of IRBP on the rate of retinol removal shows no cooperativi

114 erative response and apoptotic cell death of IRBP-specific T cells from anti-CD137 mAb-treated mice.

115 While the deletion of IRBP reduced the amplitude and slowed the kinetics of mo

116 found that Aire-dependent thymic deletion of IRBP-specific T cells relies on intercellular transfer o

117 The distribution of IRBP, as well as glycans binding peanut agglutinin (cone

118 Immunization with a low dose of IRBP and adoptive transfer of small numbers of IRBP-spec

119 and CXCR3 mediated the chemotactic effect of IRBP, while only CXCR3 was required for the chemotactic

120 IRBP), and to a major uveitogenic epitope of IRBP, peptide (p)161-180, in the absence of PTX treatmen

121 ecific for two different peptide epitopes of IRBP.

122 Thus, expression of IRBP in the periphery by DNA vaccination results in tole

123 To examine whether endogenous expression of IRBP is necessary to generate these T reg cells, we stud

124 tion relies not only on thymic expression of IRBP, but also on proper antigen processing and presenta

125 retinal HO-1 mRNA and reduced expression of IRBP.

126 d be explored in relation to the function of IRBP.

127 other specificities to inhibit generation of IRBP-specific effector T cells in a bystander fashion, i

128 blot analysis and by immunoprecipitation of IRBP labeled in vivo by intraocular injection of [(35)S]

129 n, defects that correlated with inability of IRBP-specific memory CD4-STAT3KO T cells to traffic into

130 t acts at least in part through induction of IRBP-specific, FoxP3(+)CD4(+)CD25(+) regulatory T cells.

131 We show that lack of IRBP causes delayed transfer of newly synthesized chromo

132 In addition, lack of IRBP expression solely in the thymus, even in the presen

133 Lymphocytes of IRBP-immunized mice also responded to the peptide.

134 administration of as little as 10 microg of IRBP-DNA.

135 BP and adoptive transfer of small numbers of IRBP-specific T cells from immunized tko mice caused the

136 define the temporal and spatial patterns of IRBP expression in the adult zebrafish.

137 Several new pathogenic peptides of IRBP were identified in C57BL/6 and B10.RIII mice.

138 ain 130 +/- 14 pmoles and 34 +/- 4 pmoles of IRBP, respectively.

139 scent antibodies demonstrate the presence of IRBP and IGFBP-3 in isolated retinas.

140 roper antigen processing and presentation of IRBP by thymic antigen-presenting cells.

141 These findings suggest that the processes of IRBP production and removal are coordinately regulated.

142 cells by flow cytometry and proliferation of IRBP-specific CD4(+) T cells by [(3)H]thymidine incorpor

143 dy, the relationship between the quantity of IRBP, the rate of its turnover, and the expression of it

144 ptides elicited EAU only in WT recipients of IRBP KO splenocytes.

145 responses to IRBP, an altered recognition of IRBP epitopes, and their primed T cells induced exacerba

146 mice immunized with a uveitogenic regimen of IRBP in complete Freund's adjuvant (CFA) exhibited CD25+

147 ments were used to examine the regulation of IRBP turnover by both environmental light and the light-

148 s coordinating the production and removal of IRBP are not known.

149 e these T reg cells, we studied responses of IRBP knockout (KO) versus wild-type (WT) mice.

150 portant and previously unappreciated role of IRBP in protecting the photoreceptor cells against the c

151 how that the mutation abolishes secretion of IRBP and results in formation of insoluble high molecula

152 T cells relies on intercellular transfer of IRBP between thymic stroma and bone marrow-derived antig

153 l autoimmune uveitis by adoptive transfer of IRBP-specific T cells from B6 mice.

154 The size and weight of IRBP KO mouse eyes were greater than those of the WT mou

155 However, no inhibitory effect of MSC-Exo on IRBP-specific T cell proliferation was observed.

156 to overlapping peptides derived from S-Ag or IRBP, differed from that of wild-type mice.

157 es of normal and chicken ovalbumin (OVA)- or IRBP peptide-immunized mice at day 5, 6, 7, 8, and 15 pi

158 IRBP with those of mice lacking peropsin or IRBP alone and found that the retinoid phenotype was sim

159 siently transfected with bovine rhodopsin or IRBP promoter-reporter constructs and expression constru

160 adult double-transgenic rho/rtTA-TRE/VEGF or IRBP/rtTA-TRE/VEGF mice showed little VEGF transgene exp

161 at it would be unable to transactivate PDEB, IRBP and arrestin, which were all expressed before 15 we

162 mice immunized with the uveitogenic peptide IRBP(1)(-)(2)(0) generated two types of IL-17+ T cell: o

163 were immunized with the uveitogenic peptide IRBP(1)(-)(2)(0) in either incomplete (IFA) or complete

164 mice immunized with the uveitogenic peptide IRBP(1-20) and alphabeta T cells from immunized TCR-delt

165 interphotoreceptor retinoid-binding peptide (IRBP) to develop EAU.

166 immunized with an uveitogenic IRBP peptide (IRBP(1-20)) under TH17-polarizing conditions is associat

167 The presence of physiological IRBP concentrations in the extracellular medium resulted

168 , mice induced TGF-beta expression by primed IRBP-specific effector T cells.

169 interphotoreceptor retinoid-binding protein (IRBP) 1-20 induces both CD4 and CD8 uveitogenic T cells

170 for the interphotoreceptor-binding protein (IRBP) 1-20 peptide.

171 interphotoreceptor retinoid-binding protein (IRBP) 1-20, generated both CD4+ and CD8+ autoreactive T

172 interphotoreceptor retinoid-binding protein (IRBP) 161-180-specific T cells have a strong pathogenic

173 we termed "Inverted Repeat Binding Protein (IRBP) 18" and its partner Xrp1.

174 interphotoreceptor retinoid-binding protein (IRBP) and arrestin (retinal soluble antigen, S-Ag).

175 interphotoreceptor retinoid binding protein (IRBP) and serum albumin.

176 Interphotoreceptor retinoid-binding protein (IRBP) appears to target and protect retinoids during the

177 interphotoreceptor retinoid binding protein (IRBP) can induce autoimmune uveitis in rodent models.

178 interphotoreceptor retinoid-binding protein (IRBP) fails to induce intraocular inflammation or a stro

179 interphotoreceptor retinoid-binding protein (IRBP) for retinoid delivery in the visual cycle.

180 Interphotoreceptor retinoid-binding protein (IRBP) has been considered essential for normal rod and c

181 interphotoreceptor retinoid-binding protein (IRBP) immunization, until day 21.

182 interphotoreceptor retinoid-binding protein (IRBP) in complete Freund's adjuvant.

183 interphotoreceptor retinoid-binding protein (IRBP) in the periphery, thus revoking its immune-privile

184 interphotoreceptor retinoid-binding protein (IRBP) is a proposed retinoid transporter in the visual c

185 Interphotoreceptor retinoid-binding protein (IRBP) is a retinoid-binding protein in the subretinal sp

186 Interphotoreceptor retinoid-binding protein (IRBP) is a specialized lipophilic carrier that binds the

187 interphotoreceptor retinoid-binding protein (IRBP) is expressed before being needed in its presumptiv

188 Interphotoreceptor retinoid-binding protein (IRBP) is present in the extracellular space between phot

189 Interphotoreceptor retinoid binding protein (IRBP) is the major uveitogenic retinal antigen eliciting

190 Interphotoreceptor retinoid-binding protein (IRBP) is the most abundant protein in the IPM, and it pr

191 interphotoreceptor retinoid-binding protein (IRBP) or by adoptive transfer of uveitogenic T cells and

192 interphotoreceptor retinoid-binding protein (IRBP) or cellular retinol-binding protein, suggesting th

193 interphotoreceptor retinoid binding protein (IRBP) peptide 161-180.

194 interphotoreceptor retinoid-binding protein (IRBP) peptide-specific T cells.

195 interphotoreceptor retinoid-binding protein (IRBP) peptides were evaluated for retinal infiltration o

196 interphotoreceptor retinoid-binding protein (IRBP) presumed to facilitate the traffic of chromophore,

197 interphotoreceptor retinoid-binding protein (IRBP) promoter to control the time of onset of VEGF tran

198 interphotoreceptor retinoid binding protein (IRBP) promoter-luciferase reporter construct containing

199 interphotoreceptor retinol-binding protein (IRBP) promoter.

200 Interphotoreceptor retinoid-binding protein (IRBP) secreted by photoreceptors plays a pivotal role in

201 Interphotoreceptor retinoid-binding protein (IRBP) secreted by photoreceptors plays a pivotal role in

202 interphotoreceptor retinoid-binding protein (IRBP) were compared with the effects on fully polarized

203 interphotoreceptor retinol binding protein (IRBP) were determined 1 to 2 days after exposure.

204 Interphotoreceptor retinoid binding protein (IRBP), a putative component of the visual cycle, is expr

205 CRALBP), interphotoreceptor-binding protein (IRBP), and peanut agglutinin lectin (PNA).

206 interphotoreceptor retinoid-binding protein (IRBP), and to a major uveitogenic epitope of IRBP, pepti

207 interphotoreceptor retinoid-binding protein (IRBP), in the polyclonal repertoire.

208 interphotoreceptor retinoid-binding protein (IRBP), is controlled by "natural" CD4+CD25+ regulatory T

209 interphotoreceptor retinoid-binding protein (IRBP), myelin basic protein, and bovine serum albumin fo

210 interphotoreceptor retinoid-binding protein (IRBP), relocated to bone marrow (BM).

211 nizes an insulin-responsive binding protein (IRBP), we tested the hypothesis that Sp1 interactions ar

212 Interphotoreceptor retinoid-binding protein (IRBP), which is secreted by the photoreceptors of most v

213 interphotoreceptor retinoid-binding protein (IRBP)-specific T cells and to determine the role of anti

214 interphotoreceptor retinoid-binding protein (IRBP)-specific T cells from C57BL/6 mice immunized with

215 interphotoreceptor retinoid-binding protein (IRBP).

216 interphotoreceptor retinoid-binding protein (IRBP).

217 interphotoreceptor retinol binding protein (IRBP).

218 interphotoreceptor retinoid-binding protein (IRBP).

219 interphotoreceptor retinoid-binding protein (IRBP).

220 interphotoreceptor retinoid binding protein (IRBP).

221 interphotoreceptor retinoid-binding protein (IRBP)1-20, were stimulated in vitro with various doses o

222 interphotoreceptor retinoid-binding protein (IRBP)1-20-immunized Axl and Mertk double-knockout (dko)

223 interphotoreceptor retinoid-binding protein (IRBP; an ocular antigen) or ovalbumin (OVA)-specific alp

224 interphotoreceptor retinoid binding protein (IRBP; peptide sequence 161-180) or in C57BL/6 (B6) mice

225 Here, we demonstrate that autologous rat IRBP is converted to a strong immunogen in the presence

226 serum generated against purified recombinant IRBP.

227 KO recipients of WT thymi generated reduced IRBP-specific responses, and WT recipients of KO thymi d

228 ith or without TLR stimulation, on responder IRBP-specific T cells was examined by T-cell proliferati

229 In the RPE, IRBP was associated with matrix material within phagosom

230 Disease incidence, serum IRBP antibody levels, vitreous infiltrates, retinal gran

231 ss lymphocyte proliferation, and lower serum IRBP antibody levels.

232 af1(-/-) mice, and their disease severities, IRBP specific Th1/Th17 responses, and cytokine expressio

233 In isolated cones and matrix sheets, IRBP colocalized with the peanut agglutinin binding matr

234 Retina flat mounts showed IRBP diffusely distributed in an interconnecting, lattic

235 ical entrapment within the subretinal space, IRBP is rapidly cleared from the IPM by an unknown mecha

236 munohistochemical staining visualized sparse IRBP-positive cells, undetectable by conventional assays

237 Co-transfection with AODNs suppressed IRBP promoter activity in a concentration-dependent mann

238 yltransferase reporter constructs suppressed IRBP but not SV40 promoter activity in transiently trans

239 rolipram directly to the culture suppressed IRBP-driven proliferation and IFN-gamma production by pr

240 ransfer of RPE cell-induced MDSCs suppressed IRBP-specific T-cell responses that led to EAU.

241 ly within the RPE, which does not synthesize IRBP.

242 lopment of EAU by regulation of Th1 and Th17 IRBP-specific T cells.

243 Cs to promote the activation of Th1 and Th17 IRBP-specific T cells.

244 re activation of bystander-Th17 T cells than IRBP-Th17 cells.

245 We found that IRBP removes all-trans-retinol from individual rod photo

246 We tested the hypothesis that IRBP facilitates the delivery of 11-cis-retinol to cones

247 These data indicate that IRBP is essential to normal cone function and demonstrat

248 ted Nrl(-/-)Rpe65(-/-) retinas, we show that IRBP delivers 11-cis-retinol for oxidation in cones and

249 Finally, we show that IRBP protects the isomeric state of 11-cis-retinol in th

250 These studies suggest that IRBP and S-Ag can initiate innate and, in sensitive indi

251 Our studies suggest that IRBP is not strictly confined to the subretinal space bu

252 Recent studies suggest that IRBP is short lived in the IPM (half-life, approximately

253 Together, these findings suggest that IRBP plays an important role in the delivery of 11-cis-r

254 ous studies on irbp(-/-) mice suggested that IRBP plays an insignificant role in opsin-pigment regene

255 nts and insulin responsiveness suggests that IRBP may interact directly with Sp1.

256 The IRBP-specific memory T cells (IL-7Ralpha(High)Ly6C(High)

257 formed to determine whether mutations in the IRBP gene (RBP3) are associated with photoreceptor degen

258 ealed that specific CpG dinucleotides in the IRBP gene promoter are hypomethylated in DNA from retina

259 rchitecture of the retinal allografts in the IRBP Treg cell-injected group had intact rosettes and ne

260 toreceptor cells under the regulation of the IRBP promoter (TgIRBPE2F1).

261 Asp1080Asn may alter the conformation of the IRBP protein by disrupting a conserved salt bridge.

262 served in all four homologous modules of the IRBP protein of vertebrate species and in C-terminal-pro

263 s neutralized the pathogenic activity of the IRBP-Th17 cells.

264 s the transactivation activity of CRX on the IRBP promoter.

265 ne pathway is disrupted and suggest that the IRBP-cyclin D1 mouse model may recapitulate an early ste

266 ls isolated from EAU rats in response to the IRBP antigen.

267 red the disease-inducing capability of their IRBP-specific T cells and greatly enhanced the generatio

268 Thus, IRBP does not accelerate cone pigment regeneration and i

269 Thus, IRBP is necessary for photoreceptor survival but is not

270 In addition to IRBP, the IPM also contains insulin-like growth factor-1

271 dies revealed specific hyporesponsiveness to IRBP without immune deviation, no evidence for apoptosis

272 The Th17 effector response to IRBP immunization was similar in dko mice to that in wil

273 f IFN-gamma, IL-17, and IL-10 in response to IRBP stimulation were determined.

274 ited a stronger interferon-gamma response to IRBP(161-180) restimulation in vitro.

275 distinct pattern of cytokines in response to IRBP: reduced IFN-gamma and IL-12 p40, but unchanged lev

276 he KO mice had greatly elevated responses to IRBP, an altered recognition of IRBP epitopes, and their

277 lls and decreased immunological responses to IRBP.

278 , -DQ6, and -DQ8 TG mice were susceptible to IRBP-induced EAU.

279 tina contained 75%, 18%, and 7% of the total IRBP in the eye, respectively.

280 m C57BL/6 mice immunized with an uveitogenic IRBP peptide (IRBP(1-20)) under TH17-polarizing conditio

281 s constructed containing a major uveitogenic IRBP epitope in frame with mouse IgG1 heavy chain.

282 pare epitope recognition in wild-type versus IRBP-deficient mice on both backgrounds.

283 cones in Irbp(-/-) mice to determine whether IRBP has a cone-specific visual cycle function.

284 Transfection of retinoblastoma cells with IRBP promoter CAT constructs alone produced high activit

285 -susceptible background were challenged with IRBP.

286 Furthermore, induction of EAU with IRBP-pulsed mature dendritic cells required generation o

287 tected from EAU induced by immunization with IRBP for at least 10 wk after vaccination.

288 s not present in IRBP KO mice immunized with IRBP in incomplete Freund's adjuvant (IFA), lacking myco

289 e mice (B6.Cg-Foxn1(nu)) were immunized with IRBP mixed with complete Freund's adjuvant; eyes were en

290 f Th17 cells was seen in mice immunized with IRBP(1)(-)(2)(0)/CFA, but not with IRBP(1)(-)(2)(0)/IFA.

291 lta T cells from C57BL/6 mice immunized with IRBP(1-20) produced only small amounts of IL-17 after ex

292 retinal allografts of the mice injected with IRBP Treg cells, but not in the retinal allografts of th

293 7 and 12 in the B10-RIII mice injected with IRBP-specific Treg cells; however, mice that received OV

294 for retinol removal increased linearly with IRBP concentration with a slope of 0.012 min(-1) mum(-1)

295 ized with IRBP(1)(-)(2)(0)/CFA, but not with IRBP(1)(-)(2)(0)/IFA.

296 +)CD44(+) lymphocytes were rechallenged with IRBP(161-180) in vitro to assess their antigen recall re

297 mice, adoptive transfer of WT B10.RIII with IRBP KO splenocytes did not reveal additional uveitogeni

298 ironment within the second module of Xenopus IRBP (X2IRBP) is defined.

299 iously reported crystal structure of Xenopus IRBP, the authors predict that the Asp1080-mediated cons

300 Full-length zebrafish IRBP was expressed in Escherichia coli and an antiserum