ライフサイエンスコーパス: ISH

1 ISH analyses confirmed previous findings; EtOH withdrawa

2 ISH and FISH take 3 d to perform, whereas IF/FISH takes

3 ISH and IF revealed SN1 and SN2 expression in the gangli

4 ISH arises more commonly from normal and high-normal BP

5 ISH for precursor 45S and mature 28S rRNA was accomplish

6 ISH performed on human malignant tumors revealed strong

7 ISH risks were higher than in those with high-normal BP

8 ISH risks were similar to those with high-normal BP and

9 = 2.0, average HER2 copies >/= 4.0; group 2 (ISH-positive): ratio >/= 2.0, copies < 4.0; group 3 (ISH

10 tive): ratio >/= 2.0, copies < 4.0; group 3 (ISH-positive): ratio < 2.0, copies >/= 6.0; group 4 (ISH

11 gh-normal BP (130 to 139/85 to 89 mm Hg); 3) ISH; 4) isolated diastolic hypertension (SBP <140 mm Hg

12 tive): ratio < 2.0, copies >/= 6.0; group 4 (ISH-equivocal): ratio < 2.0, copies >/= 4.0 and < 6.0; a

13 2.0, copies >/= 4.0 and < 6.0; and group 5 (ISH-negative): ratio < 2.0, copies < 4.0.

14 this study, a novel nonisotopic nucleic acid ISH method using catalyzed signal amplification and colo

15 Few systematic studies addressing ISH fixation conditions have been published.

16 An ISH compound mutation, I189T, is predicted to create a n

17 tive HER2 tests, defined as IHC 0/1+ with an ISH ratio >/= 2.0, in eight pathology centers across Can

18 After allergen and ISH challenges, expired NO was elevated relative to leve

19 rtension (> or =140 and > or =90 mm Hg), and ISH (> or =140 and <90 mm Hg).

20 Interpretable IHC and ISH scores were available in 1,212 cases from TMAs, and

21 carcinoma tissue were tested by both IHC and ISH using standardized local methods.

22 imary breast cancer were analyzed by IHC and ISH; HER2 and chromosome 17 centromere (CEP17) counts we

23 ion, we identified and characterized JHF and ISH disease-causing mutations in the capillary morphogen

24 The proportion of p16+ and ISH+ non-OP HNSCCs were similar by sex.

25 those aged > or =60 years or between SDB and ISH in either age category.

26 d the number of patient cases categorized as ISH equivocal.

27 zed reporter deposition-Ng-autometallography ISH) and a conventional indirect alkaline phosphatase me

28 In T. granulosa, AVT ISH-labeled cells were found to be widespread and locali

29 In P. shermani, the distribution of AVT ISH-labeled neurons matched that of T. granulosa, except

30 H-labeled cells was more restricted than AVT ISH labeling and was limited to regions of the preoptic

31 he SQuISH protocol against a riboprobe-based ISH procedure by comparing the mRNA expression levels in

32 e 10-17-fold higher with the riboprobe-based ISH than with the SQuISH procedure and the relative abun

33 ely 1.5-fold higher with the riboprobe-based ISH than with the SQuISH procedure, although the relativ

34 ol for studying in situ hybridization-based (ISH) expression patterns in the Allen Brain Atlas, a gen

35 ole procedure takes only 3 days and, because ISH conditions are the same for each probe tested, allow

36 he quality of each probe is evaluated before ISH using a RNA probe quantification (dot blot) assay.

37 duced mixed expired NO and suggest that both ISH and allergen-induced bronchoconstriction share patho

38 iduals with atopic asthma who underwent both ISH challenge and allergen challenge, and compared these

39 and 23.12 for the normal BP, high-normal BP, ISH, and IDH groups, respectively (all P<0.0001).

40 cluding newly available types of brightfield ISH, is proposed.

41 by IHC, and TGF-beta 1 mRNA was assessed by ISH.

42 tin receptor gene expression in rat brain by ISH using a probe complementary to mRNA for all leptin r

43 r/oval cell surface markers was confirmed by ISH and double IF analyses.

44 ytic cycle of infection were not detected by ISH; and (4) subsets of neurons were selectively vulnera

45 L. pneumophila species-specific detection by ISH, 100 and 100%.

46 Stromal THBS-2 expression was examined by ISH on tissue microarrays.

47 testing algorithms of using IHC followed by ISH for equivocal cases.

48 ially interesting genes can be identified by ISH and high-throughput image analysis techniques.

49 spectively analyzed RAR-beta mRNA levels (by ISH using a digoxigenin-labeled antisense riboprobe) in

50 ial 16S rRNA expression was also measured by ISH, and the results revealed that there was localizatio

51 ed, and all 40 patients had IL-1beta mRNA by ISH.

52 We observed by ISH and IHC that ERalpha and ERbeta mRNA and protein wer

53 centages of hepatocytes were HCV-positive by ISH that correlated with hepatocyte ultrastructural chan

54 ion (HER2 copy number or HER2/CEP17 ratio by ISH based on counting at least 20 cells within the area)

55 ened for trial entry, 40.8% were in ASCO-CAP ISH group 1, 0.7% in group 2; 0.5% in group 3, 4.1% in g

56 orter deposition in situ hybridization (CARD-ISH) analyses.

57 The CARD-ISH technique, which increased the sensitivity of the in

58 Combined ISH/LCM/PCR assays revealed that the majority of the lat

59 In conclusion, ISH using the Inform HPV III probe seems comparable to P

60 Under baseline culture conditions, ISH and RT-PCR indicated that both IL-6 and TNF alpha we

61 eased sensitivity compared with conventional ISH and was shown to be specific for HTLV-1 tax DNA.

62 With adjustment for covariates, ISH was not associated with SDB in either age category.

63 This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole

64 The HRs of developing ISH were 3.26 for normal and 4.82 for high-normal BP (bo

65 Both are believed to be allelic disorders; ISH is distinguished from JHF by its more severe phenoty

66 16 positive (p16+), 115 (48%) were HPV16 DNA ISH positive (ISH16+), and 134 (56%) were positive for a

67 distinguishable from NBF fixed tissue in DNA ISH.

68 d Hollande's solutions, and subjected to DNA ISH using biotinylated genomic probes.

69 for human papilloma virus content using DNA ISH.

70 publicly available collection of Drosophila ISH images, and show that our method achieves excellent

71 tives may compromise duplex formation during ISH.

72 ding nucleic acid melting temperature during ISH.

73 quantification with EBV tumor status by EBER-ISH.

74 served when patients were stratified by EBER-ISH.

75 EBV-DNA could serve as a surrogate for EBER-ISH and to explore its prognostic utility in HL.

76 lasma EBV-DNA is highly concordant with EBER-ISH in HL and suggest that it may have prognostic utilit

77 roL plasma yielded 96% concordance with EBER-ISH.

78 available collection of Drosophila embryonic ISH images from the Berkeley Drosophila Genome Project,

79 teraction networks from Drosophila embryonic ISH images.

80 t SPEX(2), an automatic system for embryonic ISH image processing, which can extract, transform, comp

81 munohistochemistry for p16, and fluorescence ISH for EGFR gene copy number were performed on tissue m

82 gene searches, colorimetric and fluorescent ISH image viewers, graphical displays of ISH, microarray

83 digoxigenin-labeled RNA ISH; RNA fluorescent ISH (FISH); and protein immunofluorescence (IF)-RNA FISH

84 3%) and negative predictive value (100%) for ISH positivity.

85 1.31 (P=0.40) for SDH, and 0.61 (P=0.36) for ISH.

86 94%) and negative predictive value (99%) for ISH positivity.

87 Similar results were observed for ISH+ OPSCC (P </= .01 for all).

88 eight predetermined times, and processed for ISH and immunohistochemistry.

89 P16 positivity was a good surrogate for ISH+ tumor status among OPSCC, but not a good surrogate

90 inguish systolic/diastolic hypertension from ISH.

91 descriptors to capture local statistics from ISH images and used the bag-of-words approach to build i

92 ypt sections double labeled using histone H3 ISH and BrdUrd, 92% of 1572 labeled cells exhibited both

93 It is concluded that histone H3 ISH is an accurate measure of the S-phase fraction and p

94 red in single-labeled sections by histone H3 ISH tightly correlated with that found by in vivo BrdUrd

95 Of the 4,964 patients, 1,518 had ISH (systolic blood pressure >160 mm Hg and diastolic bl

96 However, ISH detected significantly fewer HPV-positive cases in c

97 2% (23 of 95), and 19.0% (27 of 142) and HPV ISH was positive in 6.5% (six of 93), 14.6% (15 of 103),

98 sis (JHF) and infantile systemic hyalinosis (ISH) are autosomal recessive syndromes of unknown etiolo

99 refore, using Ventana in situ hybridisation (ISH), quantitative PCR assays and biomarkers of producti

100 stochemical (IHC) and in situ hybridization (ISH) analyses.

101 In situ hybridization (ISH) and immunocytochemistry (ICC) studies demonstrate t

102 d from patients using in situ hybridization (ISH) and immunocytochemistry (ICC).

103 ts distribution using in situ hybridization (ISH) and immunohistochemistry (IHC) combined with light

104 sion were assessed by in situ hybridization (ISH) and immunohistochemistry on tissue microarrays and

105 EtOH withdrawal using in situ hybridization (ISH) and KOP-R autoradiography.

106 llel approaches, dual in situ hybridization (ISH) and neurotoxic lesions of DA neurons by using 6-hyd

107 culture, we performed in situ hybridization (ISH) and polymerase chain reaction (PCR) to detect KSHV

108 nd data type [such as in situ hybridization (ISH) and supporting histology, microarray, RNA sequencin

109 stochemistry (IHC) or in situ hybridization (ISH) as a predictor of benefit from anti-HER2 therapy.

110 uate a commercialized in situ hybridization (ISH) assay for detecting human papillomavirus (HPV) DNA,

111 ethods, by DFA, by an in situ hybridization (ISH) assay, specifically designed to detect L. pneumophi

112 Real-time RT-PCR and in situ hybridization (ISH) assays confirmed these miRNA expression profiles in

113 culture-independent, in situ hybridization (ISH) assays for the detection of MAC in culture, sputum,

114 ody mRNA patterns via in situ hybridization (ISH) calls for development of accurate and automatic ima

115 he mRNA of a gene via in situ hybridization (ISH) during the development of a Drosophila melanogaster

116 sed the RNA probe for in situ hybridization (ISH) experiments.

117 ed image computing of in situ hybridization (ISH) expression patterns.

118 und to be positive by in situ hybridization (ISH) for HSV-1 latency-associated transcripts (LAT), the

119 In situ hybridization (ISH) for human papillomavirus (HPV), immunohistochemistr

120 In situ hybridization (ISH) for IL-1beta was performed using bone marrow aspira

121 DNA sequences before in situ hybridization (ISH) for localization or used ISH with radioactively-lab

122 (IHC) and additional in situ hybridization (ISH) for negative cases (IHC 0/1+).

123 unohistochemistry and in situ hybridization (ISH) for neutrophil and macrophage markers, cell adhesio

124 novel method of mRNA in situ hybridization (ISH) for the detection of transcriptionally active HR-HP

125 s high-resolution 3-D in situ hybridization (ISH) gene expression patterns in multiple developing sta

126 ion and complementary in situ hybridization (ISH) gene expression studies targeting selected genes in

127 Using in situ hybridization (ISH) histochemistry and gene-specific riboprobes, the cu

128 sis and comparison of in situ hybridization (ISH) images at laminar resolution.

129 NA is undetectable by in situ hybridization (ISH) in 50% of non-small-cell lung cancers (NSCLC).

130 us system (CNS) using in situ hybridization (ISH) in combination with a genetic knock-in approach.

131 In situ hybridization (ISH) is a powerful technique for detecting nucleic acids

132 Detection of RNAs by in situ hybridization (ISH) is a well-established technique that permits the st

133 In situ hybridization (ISH) is an essential technique for mapping gene expressi

134 In situ hybridization (ISH) measurements of c-fos and hsp70 expression were mad

135 sing a newly modified in situ hybridization (ISH) method for detecting hepatitis C virus (HCV) RNA.

136 ibe a whole-mount RNA in situ hybridization (ISH) method optimized for detection of the cellular and

137 er, we implemented an in situ hybridization (ISH) method to reveal the spatial distribution of miRNA

138 In situ hybridization (ISH) methods showed that the pattern of mRNA expression

139 nd can be detected by in situ hybridization (ISH) of viral nucleic acid (EBER) in tumor cells.

140 neity by fluorescence in situ hybridization (ISH) provides additional tissue morphology at single-cel

141 ed a highly sensitive in situ hybridization (ISH) system capable of HCV RNA and ISG mRNA detection in

142 The in situ hybridization (ISH) technique allows the sites of expression of particu

143 N were examined using in situ hybridization (ISH) techniques.

144 unohistochemistry and in situ hybridization (ISH) to characterize changes in perinatal PR expression

145 restriction, we used in situ hybridization (ISH) to detect the frequency of RNA expression for nine

146 this effect, we used in situ hybridization (ISH) to determine whether leptin alters expression of hy

147 hput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in a 96-well format.

148 We used in situ hybridization (ISH) to examine the expression of 58 genes in the dorsol

149 s this issue, we used in situ hybridization (ISH) to measure chemokine and cytokine mRNA expression l

150 ion was determined by in situ hybridization (ISH) using 35S-labelled riboprobes and immunohistochemis

151 We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in

152 In situ hybridization (ISH) using digoxigenin (DIG)-labeled RNA probes was used

153 histone H3 mRNA using in situ hybridization (ISH) was assessed as a measure of S-phase cells and comp

154 High-throughput in situ hybridization (ISH) was carried out to validate the expression of 379 g

155 could be detected by in situ hybridization (ISH) with specific antisense oligonucleotide probes in l

156 l analysis, HPV16 DNA in situ hybridization (ISH), and high-risk HPV E6/E7 mRNA ISH.

157 l analysis, HPV16 DNA in situ hybridization (ISH), and high-risk HPV E6/E7 mRNA ISH.

158 ixative of choice for in situ hybridization (ISH), and specific anecdotal cautions interdicting the u

159 immunohistochemistry, in situ hybridization (ISH), and Western and Northern blotting, we found that b

160 nglion cells (GCs) by in situ hybridization (ISH), immunofluorescence (IF), and Western blotting.

161 d by a combination of in situ hybridization (ISH), immunofluorescence, and PCR for positive-strand an

162 of enterovirus using in situ hybridization (ISH), RT-PCR, and immunohistochemistry.

163 Using in situ hybridization (ISH), we assessed the effect of body restraint stress on

164 me staining, IHC, and in situ hybridization (ISH).

165 -2 mRNA detected with in situ hybridization (ISH).

166 hemistry (IHC) and by in situ hybridization (ISH).

167 obes and quantitative in situ hybridization (ISH).

168 tochemistry (IHC) and in situ hybridization (ISH).

169 stochemistry (IHC) or in situ hybridization (ISH).

170 ection of the LATs by in situ hybridization (ISH); (3) transcripts expressed during the lytic cycle o

171 tumor cell: group 1 (in situ hybridization [ISH]-positive): HER2-to-chromosome 17 centromere ratio >

172 abeled nucleic acids (in situ hybridization; ISH) to detect transcript abundance.

173 ion, whereas isolated systolic hypertension (ISH) is common among persons over 60 years.

174 atients with isolated systolic hypertension (ISH), but the effect of treatment of ISH on LV mass is n

175 Isolated systolic hypertension (ISH), defined as systolic blood pressure (SBP) >/=140 mm

176 atients with isolated systolic hypertension (ISH).

177 Isocapnic cold air hyperventilation (ISH) is believed to cause airway narrowing through nonin

178 of the spatial pattern of gene expression in ISH images, enabled by our recently developed [Formula:

179 ver intensity levels and shape variations in ISH imagery.

180 l gene functions can be extracted from large ISH image databases such as the Allen Brain Atlas 1 and

181 Although many ISH protocols provide for quantitative analysis of indiv

182 positive as collaterally determined by mRNA ISH negativity.

183 dization (ISH), and high-risk HPV E6/E7 mRNA ISH.

184 dization (ISH), and high-risk HPV E6/E7 mRNA ISH.

185 s tested were negative for HR-HPV using mRNA ISH.

186 MSP-ISH allowed us to dissect the surprising finding that p1

187 MSP-ISH reveals that p16 hypermethylation occurs heterogeneo

188 tion-specific PCR in situ hybridization (MSP-ISH), which allows for the methylation status of specifi

189 These studies demonstrate that MSP-ISH is a powerful approach for studying the dynamics of

190 We use MSP-ISH to monitor the timing and consequences of aberrant h

191 The distribution of MST ISH-labeled cells was more restricted than AVT ISH label

192 Both types of features from multiple ISH image sections of the entire brain were then combine

193 False-negative ISH results appear to be associated with the lower copy

194 ma might be the cause for the false-negative ISH results.

195 (HPV) DNA, we compared the ability of a new ISH probe, Inform HPV III (Ventana Medical Systems, Tucs

196 The use of this sensitive nonisotopic ISH method should allow detection of other KSHV-specific

197 ent ISH image viewers, graphical displays of ISH, microarray and RNA sequencing data, Brain Explorer

198 The majority of ISH positive samples also tested HPV16 positive using se

199 Predictors of ISH included older age, female sex, and increased BMI du

200 ension (ISH), but the effect of treatment of ISH on LV mass is not known.

201 Treatment of ISH with a diuretic-based regimen reduces LV mass.

202 These results document the utility of ISH coupled with quantitative imaging techniques for the

203 e results call into question the validity of ISH results derived by the use of other gene loci, such

204 performed using an alternative assay (IHC or ISH).

205 ibroblasts derived from patients with JHF or ISH suggests that CMG2 mutations abrogate normal cell in

206 oportion of non-OP HNSCCs that were p16+ (or ISH+) increased among whites (P = .04 for trend) but not

207 Explorer also allows viewing of the original ISH images referenced from any point in a 3D data set.

208 Our ISH assay detected HR-HPV in 42% of our study population

209 PCR-ISH of HTLV-1 tax DNA in PBL from patients with HAM/TSP

210 PCR-ISH was sensitive, because a positive signal was consist

211 Also, intracellular amplification by PCR-ISH significantly increased sensitivity compared with co

212 PCR-in situ hybridization (PCR-ISH) was developed and utilized to determine the distrib

213 ted by PCR-driven in situ hybridization (PCR-ISH), and the number of viral genomes per cell was estim

214 When evaluated by reverse transcription-PCR-ISH, only a small proportion of experimentally infected

215 Quantitative ISH was performed using a betaII-tubulin-specific (33)P-

216 e have developed a standardized quantitative ISH (SQuISH) protocol that utilizes multiple radioactive

217 Using quantitative ISH, we found that AT1a mRNA expression was significantl

218 ression information from cellular-resolution ISH images.

219 Results for both DNA and RNA ISH comparing B5 and NBF fixatives were virtually identi

220 lande's solution may be used for DNA and RNA ISH under appropriate conditions.

221 varies: whole-mount, digoxigenin-labeled RNA ISH; RNA fluorescent ISH (FISH); and protein immunofluor

222 this protocol, which is similar to other RNA ISH protocols, but optimized for whole-mount Drosophila

223 Epstein-Barr encoded RNA detection using RNA ISH with fluorescein isothiocyanate-labeled oligonucleot

224 ght-field dual-colour chromogenic and silver ISH assays with an image-based computational workflow (I

225 ith the corresponding fluorescence or silver ISH result.

226 established a highly sensitive and specific ISH assay for the detection of HBV RNA, DNA, and cccDNA.

227 LC-PCR, and L. pneumophila species-specific ISH were useful as rapid tests with lung tissue.

228 The strongest ISH signal was in the hippocampal pyramidal and granule

229 olic-diastolic (SDH), and isolated systolic (ISH) hypertension are poorly understood.

230 The ISH (in-situ-hybridization)-based gene expression micro-

231 The ISH protocol uses alkaline phosphatase-conjugated digoxi

232 Currently, the ISH images are annotated with anatomical terms manually.

233 of integrated HPV16 (P = 0.008) than did the ISH+ cases.

234 natural images to extract features from the ISH images of developing mouse brain.

235 subgroup analysis of outcome results in the ISH patients.

236 Of the ISH patients, 754 were randomized to the candesartan gro

237 Our findings showed that the ISH and PCR had fair to good agreements in detecting HPV

238 Using the ISH technique IL-1beta mRNA was detectable in the plasma

239 howed a pattern that was consistent with the ISH.

240 This ISH approach provides a more direct and informative asse

241 Here we describe three ISH procedures that are optimized for Drosophila ovaries

242 We apply this pipeline to ISH data for over 16,000 genes in the Allen Brain Atlas

243 TSA-ISH proved more sensitive than DNA PCR in detecting earl

244 vaginal mucosa as early as 1 day p.i. by TSA-ISH and in retropharyngeal, tracheobronchial, or externa

245 signal-amplified in situ hybridization (TSA-ISH), and immunohistochemistry between days 1 and 12 pos

246 Two ISH family-specific truncating mutations, E220X and the

247 %) were positive for any oncogenic HPV type (ISH+).

248 hybridization (ISH) for localization or used ISH with radioactively-labeled probes to obtain adequate

249 Using ISH and RT-PCR, we verified that AT1a and AT1b receptors

250 Using ISH to open reading frame-72 (ORF 72), we localized KSHV

251 Using ISH, qPCR, and immunohistochemistry, we conclude that GR

252 llow-up, younger and middle-aged adults with ISH had higher relative risk for CVD and CHD mortality t

253 men aged 60 years and older at baseline with ISH (systolic blood pressure [BP], > or = 160 mm Hg; dia

254 Eleven cases with ISH- PCR+ results had HPV types that can be detected by

255 Five carcinoma cases with ISH- PCR+ results showed a significantly higher level of

256 PRV immunohistochemistry combined with ISH for both GlyT2 and GAD-67 mRNAs showed that at least

257 a higher proportion were p16+ compared with ISH positive (62 [10%] vs 30 [5%]; P = .001).

258 IHC signal distribution was consistent with ISH results, with transport of the protein to processes,

259 e risk for cardiovascular disease (CVD) with ISH in younger and middle-aged adults.

260 the elderly by categorizing individuals with ISH as simply hypertensive.

261 In elderly patients with ISH, antihypertensive treatment based on the ARB candesa

262 diabetic and nondiabetic older patients with ISH.

263 We prove with ISH that HRVs can enter B cells, form their viral replic

264 ost MMIHS tissue gave negative staining with ISH and variable results with ICC.

265 or CVD and CHD mortality risk for those with ISH were 1.23 (95% confidence interval [CI]: 1.03 to 1.4

266 In women with ISH, hazard ratios for CVD and CHD mortality risk were 1