ライフサイエンスコーパス: ITR

1 AAV2 Rep78 cleaved only a type 2 ITR DNA sequence, and AAV5 Rep78 cleaved only a type 5 p

2 onsisting of adeno-associated virus types 2 (ITRs and Rep) and 5 (Cap) were evaluated for muscle-dire

3 ector characterized by an AAV-2 and an AAV-5 ITR at opposite ends of the viral genome.

4 ITR sequences contained within hybrid AV2:5 ITR vectors could direct intermolecular recombination in

5 Hybrid AV2:5 ITR viruses had a significantly lower capacity to form c

6 fected cells than homologous AV2:2 and AV5:5 ITR vectors despite their similar capacity to express an

7 = 4 microg/ml for most isolates (POS, 97.6%; ITR, 96.3%; VOR, 95.9%; RAV, 93.5%).

8 ng rep68/78 outside the rep(-) vector 3' AAV ITR sequence.

9 gration into the host genome through the AAV ITR(s).

10 is found only in the hairpin form of the AAV ITR, causes a three- to eightfold reduction in the abili

11 This indicates that rescue of the AAV ITR-flanked transgene cassette from Ad.AAV-LCR-GFP is no

12 es contain only the transgene flanked by AAV ITRs, Ad packaging signals, and Ad ITRs.

13 were present; a second plasmid contained AAV ITRs flanking the reporter gene (integration cassette),

14 The presence of two intact AAV ITRs was crucial for the production of hybrid vectors an

15 e findings indicate that the presence of AAV ITRs and the Rep78 protein enhance the integration of DN

16 16-mer core sequences of the RRSs in the AAV ITRs and AAVS1 separately as query sequences and identif

17 We hypothesized that the AAV ITRs present within the hybrid vector genome could media

18 nd-specific endonuclease cuts within the AAV ITRs, and display nucleoside triphosphate-dependent heli

19 erting a transgene cassette flanked with AAV ITRs into an HSV-1 amplicon backbone, and a rep(+) HSV/A

20 olved in illegitimate recombination with AAV ITRs, deletions of which significantly reduced the exten

21 nt in the packaging of a genome with an AAV2 ITR.

22 Comparison of the TRSs in the AAV2 ITR, the AAV5 ITR, and the AAV chromosome 19 integration

23 imilar affinities for either an AAV2 or AAV5 ITR; however, comparison of terminal resolution site (TR

24 Mapping of the AAV5 ITR TRS identified a distinct cleavage site (AGTG TGGC)

25 arison of the TRSs in the AAV2 ITR, the AAV5 ITR, and the AAV chromosome 19 integration locus identif

26 s, transgene integration occurred via the Ad ITR.

27 ed by AAV ITRs, Ad packaging signals, and Ad ITRs.

28 enome can be created by breaks within the Ad ITRs, whose structure is apparently recognized by cellul

29 We also observed that adenovirus ITRs in the helper plasmid were involved in illegitimate

30 e and helper plasmids lacking the adenovirus ITRs led to complete elimination of replication-competen

31 but sustained elevation of PP and CVPP after ITR stimulation.

32 Using antibodies against ITR-1 we detected a protein of 220 kDa in C. elegans mem

33 Ectopic expression of ZF5 leads to an ITR-dependent repression of the autologous p5 promoter a

34 insect cell lines stably transfected with an ITR-flanked transgene exceeded 60 mg per 5 x 10(9) Sf9 c

35 n two myosin proteins, UNC-54 and MYO-1, and ITR-1 were identified in a yeast two-hybrid screen and s

36 those between VOR and RAV MICs (r = 0.7) and ITR and POS MICs (r = 0.4).

37 s than or equal to the ECV) for AMB, FC, and ITR, respectively, were 2 (99.8)/2 (99.2), 0.5 (94.2)/1

38 irrel monkeys with ablations of ITC; ITI and ITR (group ITRd); or ITI, ITR, and more ventral cortex,

39 ly, for A. flavus, only VOR and RAV MICs and ITR and POS MICs demonstrated statistically significant

40 were strongest between VOR and RAV MICs and ITR and POS MICs.

41 spartate type glutamate receptor subunit and ITR-1 inositol 1,4,5-triphosphate receptor.

42 ve the highest homology with the rep ORF and ITRs of AAV serotype 5 (AAV-5) (89 and 96%, respectively

43 Whereas the Rep proteins and ITRs of other serotypes are interchangeable and can be u

44 strated that, in the presence of Rep78, both ITR substrates targeted to ch-19 with similar frequency.

45 e studies demonstrate that this novel 165-bp ITR substrate is sufficient in cis for the AAV life cycl

46 epithelial cell sheets can be controlled by ITRs and suggest that such regulation may be a widesprea

47 r the human factor IX (hfIX) gene flanked by ITRs from AAV genotypes 1 through 6.

48 e integration cassette alone (GFP flanked by ITRs) produced stable fluorescent colonies at a frequenc

49 We never detected a complete ITR at a junction.

50 To examine whether the divergent ITR sequences contained within hybrid AV2:5 ITR vectors

51 Each ITR also contains a stretch of 20 nucleotides, designate

52 The terminal 125 nucleotides of each ITR form palindromic hairpin (HP) structures that serve

53 replacement of only one D sequence in either ITR restores each of these functions, but DNA strands of

54 In the absence of either factor, ITR hairpin opening was impaired, resulting in accumulat

55 pergillus isolates (from 88% of isolates for ITR to 98% of isolates for VOR and POS), MICs of each tr

56 l cortex, including perirhinal cortex (group ITR+), with visual learning in unoperated controls.

57 ys with ablations of ITC; ITI and ITR (group ITRd); or ITI, ITR, and more ventral cortex, including p

58 the capsid serotype) compared to homologous ITR vectors.

59 The use of hybrid ITR AAV vector genomes provides new strategies to manipu

60 rom this comparison demonstrated that hybrid ITR dual-vector sets had a significantly enhanced trans-

61 viral genome structures suggest that hybrid ITR vectors provide more efficient directional recombina

62 ail-to-head fashion, we generated two hybrid ITR trans-splicing vectors (AV5:2LacZdonor and AV2:5LacZ

63 To this end, we generated a novel AAV hybrid-ITR vector characterized by an AAV-2 and an AAV-5 ITR at

64 s revealed various rearrangements, including ITR deletions and amplifications of the vector and cellu

65 evaluated the involvement of the individual ITRs as well as their components in the selective viral

66 tions in different regions of the individual ITRs were constructed and examined for their potential t

67 ns of ITC; ITI and ITR (group ITRd); or ITI, ITR, and more ventral cortex, including perirhinal corte

68 thod for amphotericin B (AMB), itraconazole (ITR), voriconazole (VOR), posaconazole (POS), and ravuco

69 B (AMB), flucytosine (FC) and itraconazole (ITR) for eight Candida spp. (30,221 strains) using isola

70 ntimicrobial agents, including itraconazole (ITR), were prescribed during hospitalization and at the

71 8-A broth dilution method with itraconazole (ITR), posaconazole (POS), ravuconazole (RAV), and vorico

72 ed from the plasmid that lacked the AAV left ITR, suggesting the existence of an additional putative

73 hat the hairpin form, rather than the linear ITR, is the more frequent integration substrate.

74 anism by which the virus is able to maintain ITR sequence continuity and persist in the absence of ho

75 Molecular characterization of the mutant ITR integrants confirmed the presence of the trs mutatio

76 rovide direct evidence for the importance of ITR sequences in directing intermolecular and intramolec

77 was that B-AAV generated a greater level of ITR-initiated RNAs than did A-AAV or AAV5.

78 Stimulation of ITR neurons produced respiratory effects, but cardiovasc

79 Ephrin/VAB-1 signaling acts upstream of ITR-1 to inhibit meiotic resumption, while NMR-1 prevent

80 ed both numeric and positional variations of ITRs, which were similar to the variations in tetraploid

81 nd (vii) the AAV genomes containing only one ITR structure can be packaged into biologically active v

82 as +/-2400 copies of a derived 80 bp paired ITR structure and +/-4600 copies of solo ITRs.

83 The InsP3R protein (ITR-1) is approximately 42 % identical with known InsP3R

84 known as intimal thickness-related receptor (ITR).

85 ingle inositol 1,4,5-trisphosphate receptor (ITR) in C. elegans.

86 own that the pontine intertrigeminal region (ITR) plays an important role in respiratory regulation,

87 nvolving the pontine intertrigeminal region (ITR), has a physiological role in mediating or modulatin

88 he role of the AAV inverted terminal repeat (ITR) cis-acting sequences in targeted integration an AAV

89 d Artemis open AAV inverted terminal repeat (ITR) hairpin loops in a tissue-dependent manner.

90 site (RBS) of AAV inverted terminal repeat (ITR) in males compared with females.

91 formed by the AAV inverted terminal repeat (ITR) origins of replication, make site- and strand-speci

92 Rep) in trans, and inverted terminal repeat (ITR) sequences in cis.

93 echanisms by which inverted terminal repeat (ITR) sequences mediate intermolecular recombination of A

94 ts were within the inverted terminal repeat (ITR) sequences, which contain RBSs.

95 ence of a double-D inverted terminal repeat (ITR) structure, which implied a mechanism by which the v

96 copies of a 145-bp inverted terminal repeat (ITR) that flank the AAV genome.

97 te within the AAV5 inverted terminal repeat (ITR), and mapping of the AAV5 intron revealed that it is

98 Within the inverted terminal repeat (ITR), several base changes were identified with respect

99 bution of the interstitial telomere repeats (ITRs).

100 te flanked by AAV inverted terminal repeats (ITRs) (Ad.AAV-LCR-GFP), transduced cells were cloned and

101 sensus has 31 bp imperfect terminal repeats (ITRs) and encodes a 351 amino acid (aa) mariner transpos

102 ically to the AAV inverted terminal repeats (ITRs) and possess ATPase, helicase, and strand-specific/

103 deletions of the inverted terminal repeats (ITRs) and their flanking sequences.

104 The inverted terminal repeats (ITRs) are also unique compared to those of the other AAV

105 by using rep and inverted terminal repeats (ITRs) from AAV2 and were compared with similarly constru

106 me (ORF) and both inverted terminal repeats (ITRs) have the highest homology with the rep ORF and ITR

107 passing two viral inverted terminal repeats (ITRs) in a head-to-tail orientation, which confers a 10-

108 both ends of the inverted terminal repeats (ITRs) in the adeno-associated virus serotype 2 (AAV2) ge

109 n between (i) AAV inverted terminal repeats (ITRs) in the recombinant plasmid and (ii) AAV sequences

110 ) genome contains inverted terminal repeats (ITRs) of 145 nucleotides.

111 he right and left inverted terminal repeats (ITRs) of the transposon.

112 These inverted terminal repeats (ITRs) play an important role in AAV DNA replication and

113 has 30 bp perfect inverted terminal repeats (ITRs), and encodes a 343 amino acid (aa) mariner transpo

114 (RRSs) in the AAV inverted terminal repeats (ITRs), the AAV p5 promoter, and the preferred AAV integr

115 taining the viral inverted terminal repeats (ITRs), transgene expression cassette (human alpha 1-anti

116 played by the AAV inverted terminal repeats (ITRs), which consist of a palindromic hairpin (HP) struc

117 8/68 proteins and inverted terminal repeats (ITRs).

118 involving the AAV inverted terminal repeats (ITRs).

119 iated virus (AAV) inverted terminal repeats (ITRs).

120 rus (AAV) Rep and inverted terminal repeats (ITRs).

121 promoter, and the inverted terminal repeats (ITRs).

122 kaging sequences (inverted terminal repeats [ITRs]) derived from AAV genotypes other than 2.

123 Residual ITRs at junctions never contained more than one RBS, sug

124 mes that lacked either the left or the right ITR sequence and one of the Rep-binding sites (RBSs).

125 udal (ITC), intermediate (ITI), and rostral (ITR) subdivisions, possibly homologous to TEO, posterior

126 ning AAV inverted terminal repeat sequences (ITRs) flanking a reporter gene cassette inserted into th

127 est with inverted terminal repeat sequences (ITRs) from adeno-associated virus.

128 activity in the liver that cleaved T-shaped ITR hairpin shoulders by making nicks at diametrically o

129 t to the AAV nicking site, flanking a single ITR.

130 red ITR structure and +/-4600 copies of solo ITRs.

131 ogous to H+[or Na+]/myo-inositol symporters (ITRs), not previously studied in plants.

132 On day 67 p.t., ITR was discontinued and liposomal amphotericin B (AMB)

133 n C phenocopy these defects, confirming that ITR function is important for proper epidermal migration

134 d characterization of proteins that bind the ITR, the only viral genetic element retained in AAV2 vec

135 We explored the ITR impact on vagal reflex apnea elicited by intravenuou

136 e conclude that a physiological role for the ITR in respiration is to attenuate vagally-induced refle

137 ume more vulnerable to perturbation from the ITR.

138 Microinjections of glutamate into the ITR produced apneas, while microinjections of kynurenic

139 e receptor antagonist), and red dye into the ITR, unilaterally and bilaterally.

140 njection of glutamate (10 mM, 30 L) into the ITR.

141 Neurons of the ITR have connections with the nucleus tractus solitarius

142 findings indicate a broad involvement of the ITR in autonomic regulation, including at least cardiova

143 to eightfold reduction in the ability of the ITR to be used as a substrate for the Rep78 or MBP-Rep68

144 The 5' end of 3-base hairpin loops of the ITR was the primary target for DNA-PKcs- and Artemis-med

145 Our results suggest that the ITR strategy may be generally applicable to other system

146 CD spectra indicate that the ITR structure is largely B form DNA, although there is a

147 ely by competing with Rep for binding to the ITR.

148 nses to glutamate microinjections within the ITR in ketamine/xylazine anesthetized rats.

149 Within the ITR, the terminal resolution site and Rep binding sites

150 The ITRd monkeys had deficits on some pattern discrimination

151 The ITRd monkeys were significantly impaired on DNMS and som

152 sequences that lie outside the ITRs, and the ITRs also play a crucial role in excision of the provira

153 the contribution of the Rep proteins and the ITRs in the process of integration, we have compared the

154 hese differences in the Rep proteins and the ITRs result in a lack of cross-complementation between A

155 s also had a pair of loxP sites flanking the ITRs.

156 ge site (AGTG TGGC) which is absent from the ITRs of other AAV serotypes.

157 of an AAV5 rep/cap plasmid clone lacking the ITRs into 293 cells.

158 eplication and resolution, since each of the ITRs contains a terminal resolution site (trs) that is t

159 smids and the Rep-mediated resolution of the ITRs during AAV DNA replication, we constructed recombin

160 In all cases with the exception of the ITRs, the vector integrated intact.

161 h the AAV DNA sequences that lie outside the ITRs, and the ITRs also play a crucial role in excision

162 of the vector were mostly located within the ITRs, and cellular DNA sequences were recombined with th

163 icated probable resistance to AMB but not to ITR.

164 No complete viral ITRs were directly observed.

165 sequence of impaired resolution of the viral ITRs.