ライフサイエンスコーパス: IgG2

1 dependent on the antibody subclass (IgG1 vs IgG2).

2 dification in a recombinant variant of human IgG2.

3 in HeLa cells interacted with human IgG1 and IgG2.

4 cells, and increased P. gingivalis-specific IgG2.

5 educed IFN-gamma- and P. gingivalis-specific IgG2.

6 promote both immunopathology and protective IgG2.

7 f IgA1 or IgA2m(1) but not in the context of IgG2.

8 and immunoglobulin G1b12 [IgG1b12]) and CD4-IgG2.

9 allenge, vaccinated animals had twofold-more IgG2.

10 y, as, unlike huFcgammaRIIb, it avidly binds IgG2.

11 low anti-alpha-enolase/high anti-annexin AI IgG2.

12 maRIIb shows strongly increased affinity for IgG2.

13 Similarly, the recombinant anti-human CD14 IgG2/4 Ab, r18D11, was generated with greatly reduced Fc

14 antibodies in cell-based assays was: IgG1 > IgG2-A > IgG2 >> IgG2-B.

15 a subset showed activity differences between IgG2-A and IgG2-B.

16 IgG2-A is the known classic structure for the IgG2 subcl

17 ted with an increased hydrodynamic radius of IgG2-A relative to IgG2-B, as shown by biophysical chara

18 ns, the IgG2 disulfide isoforms converted to IgG2-A when 1 m guanidine was used, whereas IgG2-B was e

19 cells initially produce primarily one form (IgG2-A), which is rapidly converted to a second form (Ig

20 an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide conn

21 subclass, and we have named these structures IgG2-A, -B, and -A/B.

22 ain type, with IgG2lambda composed mostly of IgG2-A.

23 Multiple subvariants of the IgG2-A/B and IgG2-B structures are identified; these sub

24 IgG2-A/B represents an intermediate form, defined by an

25 which is rapidly converted to a second form (IgG2-A/B) while circulating in the blood, followed by a

26 significantly higher AFC, GC, and Th1-skewed IgG2 Ab (especially IgG2c) responses against the T cell-

27 As the IgG2 Ab response is dependent on PAF, and MDDC selective

28 Cs led to augmented Ab-forming cell, GC, and IgG2 Ab responses in Mer(-/-) mice, which were sustained

29 gG-producing Ab-forming cell, total IgG, and IgG2 Ab responses were also increased in Mer(-/-) mice.

30 ent study extended this correlation to human IgG2 Ab variants in primates.

31 ag proteins (e.g., p24) and/or production of IgG2 Abs against HIV-1 proteins.

32 onsistent with elevated levels of Th1-biased IgG2 Abs in Mer(-/-) mice.

33 dimers in three different recombinant human IgG2 Abs produced in myeloma cells.

34 of IgG2 from Ig preparations indicated that IgG2 Abs to HIV-1 p24 do not enhance phagocytosis, sugge

35 lymphocytic choriomeningitis virus-specific IgG2 Abs were dramatically decreased, whereas there was

36 In eRA, IgG2 against P. gingivalis was associated with ESR (P =

37 The observed distinction between IgG1 and IgG2 aggregation resulted from differential stability of

38 e immunization typically stimulated IgG1 and IgG2, AIT is often associated with production of IgG4.

39 ency of genotypes containing the low-binding IgG2 allele, FcgammaRIIa-R131, was significantly greater

40 IgG1 comprised 50-80% of the repertoire, and IgG2 alleles comprised < 10% in nearly all tissues.

41 By showing stronger virus-specific IgG2 and IgA responses in patients with Nef-deficient vi

42 was associated with elevated serum levels of IgG2 and IgG3 anti-dsDNA antibodies and accumulation of

43 ity of IgG1 during its normal life span; for IgG2 and IgG3 the inter-heavy chain disulfide bonds are

44 IgG2 and IgG3 were the predominant subclasses in immune

45 both species, fundamental differences in the IgG2 and IgG4 Ab subclasses were found between the two s

46 In humans, IgG2 and IgG4 adapted a silent Fc region with weak bindi

47 IgG2 and IgG4 anti-GXM prolonged the lives of infected B

48 and IgG3, respectively, but no increase with IgG2 and IgG4 anti-RhD Abs.

49 ons, whereas, in contrast, cynomolgus monkey IgG2 and IgG4 display strong effector function as well a

50 IgG2 and IgG4 isotypes have significantly lower binding

51 ings, in vitro and in vivo results for human IgG2 and IgG4 obtained in the cynomolgus monkey have to

52 the gamma heavy chain was restricted to the IgG2 and IgG4 subclasses, suggesting the binding of mono

53 dies of many different IgG subclasses (IgG1, IgG2 and IgG4) are used in the treatment of various canc

54 subclass, has a half-life as long as that of IgG2 and IgG4, binds the FcgammaR receptor, and activate

55 nt and these EC antibodies were enriched for IgG2 and IgG4, noncomplement activating subclasses.

56 etermination showed AECAs to be enriched for IgG2 and IgG4, subclasses that do not activate complemen

57 uce heterogeneous disulfide bonding in human IgG2 and maintain in vitro activity.

58 high anti-alpha-enolase/low anti-annexin AI IgG2 and patients with low anti-alpha-enolase/high anti-

59 y and the levels of S. mansoni worm IgG1 and IgG2 and Plasmodium falciparum IgG1 and IgG4.

60 Bi-trait variance component analyses of IgG2 and quantitative measures of AgP indicate that diff

61 IgG antibodies to PEG-uricase, mostly IgG2 and specific for PEG, developed in 9 patients, who

62 evels of serum anti-alpha-enolase (>15 mg/L) IgG2 and/or anti-annexin AI (>2.7 mg/L) IgG2 were detect

63 cations demonstrated that immunoglobulin G2 (IgG2) and IgA antibodies arose from the same somatically

64 Conduits attenuated immunoglobulin G2 (IgG2) and IgA class switching in systemic and intestinal

65 P are recognized by serum immunoglobulin G2 (IgG2) and stimulate memory T-lymphocyte proliferation an

66 immunoglobulin (Ig) G, its subtypes IgG1 and IgG2, and IgA and IgM in vaccinated animals.

67 markable IgG responses that are dominated by IgG2, and IgG2 is IFN-gamma-dependent and is promoted by

68 es had elevated levels of Env-specific IgG1, IgG2, and IgG3 Abs compared with males.

69 ly useful properties compared with the IgG1, IgG2, and IgG3 subclasses.

70 Immunoglobulin G subclasses IgG1, IgG2, and IgG4 samples were first stressed in protonated

71 ) mice had improved humoral responses (IgG1, IgG2, and IgM), a result that further explains the strai

72 e that express human immunoglobulin M [IgM], IgG2, and kappa) which were immunized with a C. neoforma

73 in a solution of human monoclonal antibody, IgG2, and the effects of human serum albumin, a major bl

74 nd Porphyromonas gingivalis are dominated by IgG2, and these IgG2 responses are associated with reduc

75 B cells into PCs, induced class switching to IgG2, and was reproducible in cocultures with neutrophil

76 ranulocytes, the main effector cells against IgG2- and IgG4-opsonized bacteria and parasites, do not

77 Anti-DNA IgG3 was the unique non-IgG2 anti-DNA deposit, and anti-C1q IgG4 was mainly dete

78 es correlated with plasma levels of IgG1 and IgG2 anti-HIV-1 p24 and, notably, correlated inversely w

79 ssue of the JCI that recognition of IgG1 and IgG2 antibodies by FcgammaRIII and FcgammaRIV receptors

80 All IgG2 antibodies displayed the same disulfide conversion,

81 uctural isoforms are present in native human IgG2 antibodies isolated from myeloma plasma and from no

82 We found that IgG2 antibodies predominated in the response to vaccinat

83 However, with the exception of IgG2 antibodies to gp41, HLA status was not associated w

84 d-induced aggregation of monoclonal IgG1 and IgG2 antibodies was studied at pH 3.5 as a function of s

85 Human monoclonal IgG1 and IgG2 antibodies were designed with identical antigen bin

86 A pharmacokinetics study of two mutant IgG2 antibodies with increased FcRn binding affinity ind

87 both human immunoglobulin gamma 1 (IgG1) and IgG2 antibodies.

88 due in the C(H)1 constant domain of IgG1 and IgG2 antibodies.

89 d by excess production of O-antigen-specific IgG2 antibodies.

90 uctural variants of human immunoglobulin G2 (IgG2) antibodies was recently the subject of two copubli

91 E(2) and the induction of immunoglobulin G2 (IgG2) antibodies.

92 tolerability of AMG 145, a human monoclonal IgG2 antibody against PCSK9, in stable patients with hyp

93 o additional monoclonal antibody species: an IgG2 antibody and an IgG1 antibody conjugate.

94 nstrates that the disulfide structure of the IgG2 antibody is dynamic in vivo, on a time scale simila

95 in skin test strongly boosted IgG, IgG1, and IgG2 antibody responses, particularly against MPB83 and

96 Recently, the human IgG2 antibody subclass was found to possess multiple str

97 Denosumab is a fully human monoclonal IgG2 antibody that binds RANKL, inhibiting its activity.

98 used to separate populations of recombinant IgG2 antibody that were created as a result of prolonged

99 Applying our approach to a stressed IgG2 antibody, 10 cross-linked peptides were discovered

100 ully human monoclonal immunoglobulin gamma2 (IgG2) antibody panitumumab against human epidermal growt

101 interferon secretion and immunoglobulin G2 (IgG2) antibody titers.

102 As high levels of circulating IgG2 are correlated with less severe disease, the propen

103 y and determine whether genes that influence IgG2 are the same genes that influence disease susceptib

104 Notably, IgG2 autoantibodies against alpha-enolase and annexin AI

105 how a multiantibody composition in LN, where IgG2 autoantibodies against alpha-enolase and annexin AI

106 IgG2 autoantibodies against DNA, histones (H2A, H3, and

107 Anti-H3, anti-DNA, and anti-C1q IgG2 autoantibodies were also prevalent in LN serum, whi

108 e, the connectivity of a novel subvariant of IgG2-B containing an intrachain disulfide linkage in the

109 IgG2-B is a structure defined by a symmetrical arrangeme

110 Multiple subvariants of the IgG2-A/B and IgG2-B structures are identified; these subvariants of e

111 IgG2-A when 1 m guanidine was used, whereas IgG2-B was enriched in the absence of guanidine.

112 owed by a slower conversion to a third form (IgG2-B).

113 ed hydrodynamic radius of IgG2-A relative to IgG2-B, as shown by biophysical characterization.

114 owed activity differences between IgG2-A and IgG2-B.

115 ll-based assays was: IgG1 > IgG2-A > IgG2 >> IgG2-B.

116 e quality of the immune response (i.e., IgG1/IgG2 balance and mucosal IgA and IL-17 secretion) was de

117 ll, our results highlight KTN0073 as a novel IgG2-based MET mAb that acts through exon 14-independent

118 ensity malaria, whereas individuals with the IgG2-binding Fc gamma RIIa-His/His131 genotype are at in

119 The present study suggests that the IgG2-binding Fc gamma RIIa-His/His131 genotype is associ

120 iodontitis (LAgP) produce elevated levels of IgG2 both in vivo and in vitro.

121 n IgG1 antibody and a Cys-232 --> Ser mutant IgG2, both of which are homogeneous with respect to disu

122 at the lower binding of CD32A(R) not only to IgG2 but also to IgG1 and IgG3 might be responsible for

123 A novel FcgammaR that binds IgG2 but not IgG1 has just been identified, potentially

124 on of IFN-gamma also restored IND-suppressed IgG2 but not IgG1.

125 s as the glomerular deposits (mostly IgG1 or IgG2), but only one patient had myeloma.

126 ignificantly lower binding not only to human IgG2, but also to IgG1 and IgG3 subtypes.

127 all the disulfide bridges and show that the IgG2 C H1 and C-terminal C L cysteine residues are eithe

128 Furthermore, nephritogenic monoclonal IgG2 (clone H147) derived from lupus-prone MRL-lpr/lpr m

129 4, both of which are present in the IgG1 and IgG2 constant domain sequences, and Asn-35, which was pr

130 Grafting the mAb variable regions onto the IgG2 constant region dramatically enhanced the tumor inh

131 robably due to the enhanced stability of the IgG2 construct, than previously observed with (131)I-mur

132 The hinge region of human IgG2 contains four cysteine residues involved in disulfi

133 ch were primarily immunoglobulin (Ig) G1 and IgG2, cross-reacted with oligodendrocytes, perivascular

134 asurable levels of D-cysteines were found on IgG2 cysteines in the hinge region, both with monoclonal

135 ss IV nephritis and in patients with intense IgG2 deposition.

136 The IgG2 dimers are in contrast to the noncovalent IgG dimer

137 The potential role of IgG2 dimers in immunity against carbohydrate Ags is disc

138 several normal sera revealed the presence of IgG2 dimers.

139 The complication of IgG2 disulfide connections demands advances in technique

140 crystal structure analysis, we propose that IgG2 disulfide exchange is caused by the close proximity

141 structural and functional properties of the IgG2 disulfide isoforms and compared them to IgG1.

142 Under reduction-oxidation conditions, the IgG2 disulfide isoforms converted to IgG2-A when 1 m gua

143 the fundamental framework of three distinct IgG2 disulfide isoforms recently described.

144 -containing disulfide peptides produced from IgG2 disulfide isoforms.

145 Here we show that these IgG2 disulfide linkages interconvert while circulating i

146 in this paper reveal that the population of IgG2 disulfide structural variants is yet more complex t

147 Thus, changes to the IgG2 disulfide structure provide a marker of the protein

148 Human IgG2 exists as a mixture of disulfide-linked structural

149 No reactivity against IdeS-generated IgG2-F(ab')2s was detected.

150 of human decay accelerating factor linked to IgG2 Fc have been developed.

151 fied Ig binding, with a preference for human IgG2 Fc, and localized the IgG-binding region to a highl

152 tion of TFSS antigens by CD4+ T cells and by IgG2 from cattle immunized with the protective outer mem

153 reatest in HIV controllers, and depletion of IgG2 from Ig preparations indicated that IgG2 Abs to HIV

154 ce) and expressing either immunoglobulin G2 (IgG2) (G2 mice) or IgG4 (G4 mice).

155 t region (G1)) or without effector function (IgG2/G4 fusion constant region (G2G4)) exhibited high an

156 s provides strong evidence that the observed IgG2 gas-phase conformers are related to disulfide bond

157 2 doses of PsA-TT had the greatest IgG1 and IgG2 GMCs of 125.23 microg/mL and 36.12 microg/mL, respe

158 Group A-specific IgG1 and IgG2 GMCs remained greater in the PsA-TT group than in t

159 es in cell-based assays was: IgG1 > IgG2-A > IgG2 >> IgG2-B.

160 unlike other immunoglobulin isotypes, human IgG2 (h2) imparts FcgammaR-independent agonistic activit

161 RIIa-R131, the variant with low affinity for IgG2, has high affinity for CRP.

162 d serum-mediated killing of P. aeruginosa by IgG2 have poorer respiratory function than infected pati

163 e used variance component analyses to assess IgG2 heritability and determine whether genes that influ

164 Production of IgG1, IgG2, IFN-gamma, and PGE2 was monitored by enzyme-linked

165 istamine, antibody activity (IgG, IgE, IgG1, IgG2, IgA), cytokines (IL-4, IFN-gamma, IL-12p70, IL-10,

166 gG1 sequence with those of other subclasses (IgG2, IgG3 and IgG4) showed that these aggregation-prone

167 shows distinct properties compared with the IgG2, IgG3, and IgG4 subclasses and is the most exploite

168 DNA technology, 5C12 isotype variants (IgG1, IgG2, IgG3, and IgG4) and antibody fragments [Fab, F(ab'

169 dopeptidase activity were obtained for IgG1, IgG2, IgG3, and IgG4.

170 tigen (SWA)-specific immunoglobulin (Ig) G1, IgG2, IgG3, IgG4, interleukin (IL)-4, and IL-5 increased

171 uals were rarely positive for anti-chlamydia IgG2, IgG4 or IgA2.

172 Therefore, an inert human IgG2/IgG4 hybrid C region was chosen for an rMil2.

173 ody-related products is increasing including IgG2/IgG4 subclasses and engineered Fc regions to enhanc

174 f the feline heavy chains of IgG1a (IGHG1a), IgG2 (IGHG2), and IgA (IGHA), and the light chains (lamb

175 on of the inflammatory factors such as IgG1, IgG2, IgM, IL-6 and PMPhi phagocytosis, stimulation of s

176 vity similar to that of the negative control IgG2 in a CD20(+) human Raji lymphoma cell line.

177 creased activity of the IgG1 relative to the IgG2 in blocking interleukin-1beta ligand from binding t

178 olony-stimulating factor selectively induced IgG2 in cultures of pokeweed mitogen-stimulated NP leuko

179 lization of alpha-enolase or annexin AI with IgG2 in glomeruli.

180 stricted in isotype to IgM and predominantly IgG2 in humans and IgM, and IgG3 in mice.

181 is, and proteins recognized by total IgG and IgG2 in immune sera of outer membrane-vaccinated cattle

182 subjects, likely because of the presence of IgG2 in the complexes.

183 etric mean concentrations (GMCs) of IgG1 and IgG2 in the PsA-TT group were 21.73 microg/mL and 6.27 m

184 F) from LagP patients and the high levels of IgG2 in their serum and GCF that is reactive with A. act

185 IgG2 is elevated in localized but not in generalized agg

186 gG responses that are dominated by IgG2, and IgG2 is IFN-gamma-dependent and is promoted by dendritic

187 to the receptor, suggesting that some of the IgG2 isoforms had lower activity.

188 Furthermore, the IgG2 isoforms were shown to interconvert in whole blood

189 Recent evidence suggests that the IgG2 isotype is not completely devoid of effector functi

190 the HI activity of Cb/E-specific MAbs of the IgG2 isotype isolated from the primary response was enha

191 noclonal antibody characterized by a mutated IgG2 isotype, lack of binding to Fcgamma-receptors, and

192 mation by interacting with IgG1, even though IgG2 isotypes tend to be more pathogenic.

193 cyte responses and specific Abs dominated by IgG2 isotypes.

194 Both IgG2,kappa and IgG2,lambda can form dimers.

195 Both IgG2,kappa and IgG2,lambda can form dimers.

196 icate that different genes appear to control IgG2 levels and disease susceptibility.

197 Anti-H3 and anti-alpha-enolase IgG2 levels had the most remarkable increase in LN serum

198 cytokine levels in CD4 T cells and increased IgG2 levels in sera.

199 feration and IL-2 production, serum IgG1 and IgG2 levels of both auto- and heteroantibodies, and solu

200 By contrast, heritability of IgG2 levels was estimated to be 38% and highly significa

201 l phenotype (generating 2.5-fold higher IgG1/IgG2 levels).

202 mplishes the reduced tryptic digestion of an IgG2 mAb in a mildly acidic condition (pH 6.0) with half

203 d in the separation of disulfide isoforms of IgG2 mAbs by CZE.

204 her than the respective IgG1 MAbs, while the IgG2 MAbs had the least activity.

205 gy to produce fully human immunoglobulin G2 (IgG2) MAbs from B cells of an individual post-Staphyloco

206 gG1 decreased activity, whereas switching to IgG2 markedly increased activity.

207 uggesting that the in vivo activity of human IgG2 may be dependent on the distribution of isoforms.

208 DEK autoantibodies (IgG2) may activate the complement cascade, primarily rec

209 The IgG1:IgG2 mean ratio decreased following successive vaccinati

210 demonstrated a significant increase in IgG1:IgG2 mean ratio, indicative of the T-cell-dependent resp

211 ized AgP-affected members (274 subjects with IgG2 measurements).

212 tween different Gram-negative bacteria, this IgG2-mediated impairment of killing may operate in other

213 ently reported to take place in serum for an IgG2 molecule and resulted in predictable mature isoform

214 V(L) domain sequence of a recombinant human IgG2 molecule.

215 IgG2 molecules are organized differently from that model

216 t studies of the covalent structure of human IgG2 molecules.

217 present the detailed disulfide structure of IgG2 molecules.

218 reproducible separation of multiple IgG1 and IgG2 monoclonal antibodies (mAbs) was obtained with a lo

219 activity studies were extended to additional IgG2 monoclonal antibodies with various antigen targets.

220 acterization of disulfide variants in intact IgG2 monoclonal antibodies.

221 ailed analysis showed that recombinant human IgG2 monoclonal antibody could be partially resolved int

222 Figitumumab is a fully human IgG2 monoclonal antibody targeting the insulin-like grow

223 Nonenzymatic glycation of an IgG2 monoclonal antibody was studied using affinity chro

224 /MS/MS analysis of a tryptic digestion of an IgG2 monoclonal antibody, 1712 peptide ions were identif

225 h FcgammaRIIa 131H is known to bind IgG1 and IgG2 more avidly, no such differences in affinity are kn

226 rom animal experiments have shown that human IgG2/mouse chimeric antitenascin 81C6 (ch81C6) monoclona

227 Following mutagenesis, several IgG2 mutants with increased binding affinity to human Fc

228 Here we investigated to what extent human IgG2 N-terminal glutamate converts to pE in vivo.

229 compared with a wild-type IgG1 (IgG1/wt) or IgG2 of identical antigen specificity.

230 ino acid substitutions from the Fc region of IgG2 or IgG4 antibodies, reduced but did not eliminate D

231 er monocyte infiltration into the graft than IgG2 or IgG4 due to enhancement by FcgammaR interactions

232 ng domains were each paired with human IgG1, IgG2, or IgG3.

233 cacy superior to that observed with IgG1/wt, IgG2, or IgG4 of identical antigen specificity.

234 ing of HIV-1 by neutralizing mAb b12 and CD4-IgG2 (PRO-542) blocked both localized infection and vira

235 eriodontitis, a disease associated with high IgG2 production and a propensity of monocytes to differe

236 Indomethacin treatment inhibited IgG1 and IgG2 production, and PGE2 restored both immunoglobulins

237 ependent on PAF, and MDDC selectively induce IgG2 production, we predicted that PAF levels would be h

238 ocyte-derived dendritic cells (mDCs) promote IgG2 production.

239 n (IFN)-gamma (a Th-1 mediator) both promote IgG2 production.

240 ved greater persistence and higher titers of IgG2 protective antibodies.

241 The IgG2 proved to have similar modifications to Infliximab

242 Immune responses are dominated by IgG2 reactive with bacterial surface carbohydrates.

243 fluid and high titers of IFN-gamma-dependent IgG2 reactive with P. gingivalis in gingival crevicular

244 Anti-alpha-enolase IgG2 recognized specific epitopes of alpha-enolase and d

245 e characterized a series of Cys-->Ser mutant IgG2 recombinant monoclonal antibodies, focused on the f

246 ed mice had less histamine and IgG1 and more IgG2-related antibodies indicating a bias toward the typ

247 saline and isotype controls (human and mouse IgG2) remained unchanged.

248 in half the MCB patients mainly exhibited an IgG2 response, while IgG1 dominated in the others.

249 ts develop a substantial anti-gp120-specific IgG2 response.

250 gingivalis are dominated by IgG2, and these IgG2 responses are associated with reduced extent and se

251 Anti-P. gingivalis IgG2 responses were enhanced by dendritic cells, and rem

252 N-gamma production and help explain elevated IgG2 responses.

253 and that this IFN-gamma selectively promotes IgG2 responses.

254 rIL-1beta, rIL-6, or rIL-12 did not restore IgG2 responses.

255 Human immunoglobulin G2 (IgG2) responses are gamma interferon (IFN-gamma) depende

256 han the individual fragments, similar to the IgG2 results.

257 However, IgG2 samples displayed a wide range of affinities, indic

258 ls of immunoglobulin M (IgM), IgG, IgG1, and IgG2 serum antibodies against ruminant C. abortus in a c

259 These results show that IgG2 structure is significantly different from the conve

260 Humans with IgG2 subclass deficiency are susceptible to sinus and pu

261 gG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab do

262 ng report, we have identified that the human IgG2 subclass exists as an ensemble of distinct isoforms

263 mmunoglobulin G (IgG) antibodies, of IgG3 or IgG2 subclass, against SPAN-Xb were detectable in the se

264 Little is known about regulation of the IgG2 subclass, although prostaglandin E2 (PGE2) (a media

265 ee main types of structures within the human IgG2 subclass, and we have named these structures IgG2-A

266 e presently accepted structure for the human IgG2 subclass, we also found major structures that diffe

267 ident in a recombinant human antibody of the IgG2 subclass.

268 of a therapeutic monoclonal antibody of the IgG2 subclass.

269 pper hinge cysteine residues specific to the IgG2 subclass.

270 feature of antibodies belonging to the human IgG2 subclass.

271 ffect of endogenous IgG, especially from the IgG2 subclass.

272 sity to aggregate compared with those of the IgG2 subclass.

273 eriments that utilized molecules of IgG1 and IgG2 subclasses with varying levels of C(H)2 glycosylati

274 as unchanged, with bias towards the IgG1 and IgG2 subclasses.

275 We have engineered both human IgG1 and IgG2 subtypes, with minimal point mutations, to form ful

276 ation with PsACWY, indicating a shift toward IgG2, suggestive of the T-cell-independent immune respon

277 tiation may be related to the high levels of IgG2 that are observed in the sera of LJP subjects.

278 and II clinical trials with AMG-162, a human IgG2 that binds receptor activator of nuclear factor kap

279 ents, and these patients have high levels of IgG2 that is reactive with Actinobacillus actinomycetemc

280 NAb response correlated with virus-specific IgG2 titers and that the in vivo neutralization potency

281 erity of asthma with anti-G protein IgG1 and IgG2 titers was observed.

282 Depletion of IgG2 to O-antigen restores the ability of sera to kill s

283 We suggest that excessive binding of IgG2 to O-antigen shields the bacterium from other antib

284 For IgG2, two pepsin cleavage sites were identified; anti-hi

285 d AgP, the model still explained only 19% of IgG2 variance.

286 for proteins and its possible origins in the IgG2 variant are discussed.

287 In pre-RA, P. gingivalis-specific IgG2 was associated with ACPAs (P = 0.049) and disease s

288 Only 16% of the variance in IgG2 was attributable to age, race, and smoking.

289 In in vitro killing assays, anti-ChoP IgG2 was effective against some clinical isolates of non

290 IgG2 was the predominant anti-PEG IgG subclass.

291 P-specific human antibodies (mainly IgG1 and IgG2) was detected in all immunized mice.

292 Isotype diversification (toward IgG2) was greatest in HIV controllers, and depletion of

293 P. gingivalis IgG1 and IgG2 were analyzed.

294 contrast, AHA levels against pepsin-cleaved IgG2 were comparable.

295 g/L) IgG2 and/or anti-annexin AI (>2.7 mg/L) IgG2 were detected in most patients with LN but not pati

296 strong increase in IgG4 and some increase in IgG2 were observed throughout the study, while productio

297 occal capsular polysaccharide (total IgG and IgG2) were assessed at baseline and 16 wk.

298 HH platelets were also sensitive to IgG2, which in contrast, failed to inhibit the response

299 reduce disulfide bond heterogeneity in human IgG2 while preserving the activity of this therapeutical

300 re, the isoforms are present in native human IgG2 with either kappa or lambda light chains, although